LOCUS       RC218925                8256 bp    DNA     circular  SYN 
DEFINITION  MAGI3 (Myc-DDK-tagged)-Human membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 2
ACCESSION   NM_152900
VERSION     NM_152900.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4413..4504 
                     /label="Myc-DDK"  
     misc_feature    4534..5119 
                     /label="PolyA signal"  
     misc_feature    4591..4610 
                     /label="Restriction Enzyme"  
     misc_feature    5395..6067 
                     /label="ColE1"  
     misc_feature    6497..7291 
                     /label="Kan/Neo"  
     misc_feature    7326..7628 
                     /label="SV40 ori"  
     misc_feature    7714..8191 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4395..4401 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4395 
                     /label="MAGI3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGAAGACG CTGAAGAAGA AGAAGCACTG GCTCAGCAAG GTGCAGGAGT 
     1081 GCGCCGTGTC CTGGGCCGGG CCCCCGGGCG ACTTCGGCGC GGAGATCCGC GGTGGCGCGG 
     1141 AGCGTGGCGA GTTCCCCTAC CTGGGGCGGC TCCGCGAGGA GCCCGGCGGG GGCACCTGCT 
     1201 GCGTCGTCTC GGGCAAGGCG CCCAGCCCAG GCGATGTGCT GCTGGAGGTA AACGGGACGC 
     1261 CTGTCAGCGG GCTCACCAAC CGGGACACCC TGGCTGTCAT CCGCCACTTC CGCGAGCCCA 
     1321 TCCGTCTCAA GACTGTGAAA CCAGGCAAAG TCATTAATAA AGATTTGCGG CATTACCTAA 
     1381 GTCTTCAGTT TCAAAAAGGA TCAATTGACC ACAAACTGCA GCAAGTGATC AGAGATAATC 
     1441 TCTACTTGAG AACCATTCCA TGCACTACAA GGGCCCCCAG GGATGGAGAA GTACCAGGAG 
     1501 TGGATTATAA TTTCATTTCC GTTGAACAGT TCAAAGCACT GGAAGAGAGT GGAGCATTGT 
     1561 TAGAAAGTGG GACATATGAT GGAAACTTCT ATGGAACTCC CAAGCCTCCA GCAGAACCCA 
     1621 GCCCTTTTCA GCCAGATCCA GTTGATCAAG TCCTCTTTGA TAATGAGTTT GATGCAGAAT 
     1681 CTCAAAGAAA ACGAACGACA TCTGTCAGCA AGATGGAAAG AATGGATAGC TCTCTTCCTG 
     1741 AAGAGGAAGA AGATGAGGAC AAGGAAGCTA TTAATGGCAG TGGAAACGCA GAAAACAGAG 
     1801 AGAGGCATTC TGAGTCATCT GACTGGATGA AGACTGTTCC AAGTTACAAC CAAACAAATA 
     1861 GCTCCATGGA CTTTAGAAAT TATATGATGA GAGATGAGAC TCTGGAACCA CTGCCCAAAA 
     1921 ACTGGGAAAT GGCCTACACT GACACAGGGA TGATCTACTT CATTGACCAC AATACCAAGA 
     1981 CAACCACCTG GTTGGATCCT CGTCTTTGTA AGAAAGCCAA AGCCCCTGAA GACTGTGAAG 
     2041 ATGGAGAGCT TCCTTATGGC TGGGAGAAAA TAGAGGACCC TCAGTATGGG ACATACTATG 
     2101 TTGATCACCT TAACCAGAAA ACCCAGTTTG AAAATCCAGT GGAGGAAGCC AAAAGGAAAA 
     2161 AGCAGTTAGG ACAGGTTGAA ATTGGGTCTT CAAAACCAGA TATGGAAAAA TCACACTTCA 
     2221 CAAGAGATCC ATCCCAGCTT AAAGGTGTCC TTGTTCGAGC ATCACTGAAA AAAAGCACAA 
     2281 TGGGATTTGG TTTTACTATT ATTGGTGGAG ATAGACCTGA TGAGTTCCTA CAAGTGAAAA 
     2341 ATGTGCTGAA AGATGGTCCC GCAGCTCAGG ATGGGAAAAT TGCACCAGGC GATGTTATTG 
     2401 TAGACATCAA TGGCAACTGT GTCCTCGGTC ACACTCATGC AGATGTTGTC CAGATGTTTC 
     2461 AATTGGTACC TGTCAATCAG TATGTAAACC TCACTTTATG TCGTGGTTAT CCACTTCCTG 
     2521 ATGACAGTGA AGATCCTGTT GTGGACATTG TTGCTGCTAC CCCTGTCATC AATGGACAGT 
     2581 CATTAACCAA GGGAGAGACT TGCATGAATC CTCAGGATTT TAAGCCAGGA GCAATGGTTC 
     2641 TGGAGCAGAA TGGAAAATCG GGACACACTT TGACTGGTGA TGGTCTCAAT GGACCATCAG 
     2701 ATGCAAGTGA GCAGAGAGTA TCCATGGCAT CGTCAGGCAG CTCCCAGCCT GAACTAGTGA 
     2761 CTATCCCTTT GATTAAGGGC CCTAAAGGGT TTGGGTTTGC AATTGCTGAC AGCCCTACTG 
     2821 GACAGAAGGT GAAAATGATA CTGGATAGTC AGTGGTGTCA AGGCCTTCAG AAAGGAGATA 
     2881 TAATTAAGGA AATATACCAT CAAAATGTGC AGAATTTAAC ACATCTCCAA GTGGTAGAGG 
     2941 TGCTAAAGCA GTTTCCAGTA GGTGCTGATG TACCATTGCT TATCTTAAGA GGAGGTCCTC 
     3001 CTTCACCAAC CAAAACTGCC AAAATGAAAA CAGATAAAAA GGAAAATGCA GGAAGTTTGG 
     3061 AGGCCATAAA TGAGCCTATT CCTCAGCCTA TGCCTTTTCC ACCGAGCATT ATCAGGTCAG 
     3121 GATCCCCAAA ATTGGATCCT TCTGAGGTCT ACCTGAAATC TAAGACTTTA TATGAAGATA 
     3181 AACCACCAAA CACCAAAGAT TTGGATGTTT TTCTTCGAAA ACAAGAGTCA GGGTTTGGCT 
     3241 TCAGGGTGCT AGGAGGAGAT GGACCTGACC AGTCTATATA TATTGGGGCT ATTATTCCCC 
     3301 TGGGAGCAGC TGAGAAAGAT GGTCGGCTCC GCGCAGCTGA TGAACTAATG TGCATTGATG 
     3361 GAATTCCTGT TAAAGGGAAA TCACACAAAC AAGTCTTGGA CCTCATGACA ACTGCTGCTC 
     3421 GAAATGGCCA TGTGTTACTA ACTGTCAGAC GGAAGATCTT CTATGGAGAA AAACAACCCG 
     3481 AGGACGACAG CTCTCAGGCC TTCATTTCAA CACAGAATGG ATCTCCCCGC CTGAACCGGG 
     3541 CAGAGGTCCC AGCCAGGCCT GCACCCCAGG AGCCCTATGA TGTTGTCTTG CAACGAAAAG 
     3601 AAAATGAAGG ATTTGGCTTT GTCATCCTCA CCTCCAAAAA CAAACCACCT CCAGGAGTTA 
     3661 TTCCTCATAA AATTGGCCGA GTCATAGAAG GAAGTCCGGC TGACCGCTGT GGAAAACTGA 
     3721 AAGTTGGAGA TCATATCTCT GCAGTGAATG GGCAGTCCAT TGTTGAACTG TCTCATGATA 
     3781 ACATTGTTCA GCTGATCAAA GATGCTGGTG TCACCGTCAC ACTAACGGTC ATTGCTGAAG 
     3841 AAGAGCATCA TGGTCCACCA TCAGGAACAA ACTCAGCCAG GCAAAGCCCA GCCCTGCAGC 
     3901 ACAGGCCCAT GGGACAGTCA CAGGCCAACC ACATACCTGG GGACAGAAGT GCCCTAGAAG 
     3961 GTGAAATTGG AAAAGATGTC TCCACTTCTT ACAGACATTC TTGGTCAGAC CACAAGCACC 
     4021 TTGCACAGCC TGACACCGCA GTAATTTCAG TTGTAGGCAG TCGGCACAAT CAGAACCTTG 
     4081 GTTGTTATCC AGTAGAGCTG GAGAGAGGCC CCCGGGGCTT TGGATTCAGC CTCCGAGGGG 
     4141 GGAAGGAGTA CAACATGGGG CTGTTCATCC TTCGTCTTGC TGAAGATGGT CCTGCCATCA 
     4201 AAGATGGCAG AATTCATGTT GGTGACCAGA TTGTTGAAAT CAATGGGGAA CCTACACAAG 
     4261 GAATCACACA TACTCGAGCA ATTGAGCTCA TTCAGGCTGG TGGAAATAAA GTTCTTCTTC 
     4321 TTTTGAGGCC AGGAACTGGC TTGATACCTG ACCATGGTTT GGCTCCTTCC GGTCTGTGCT 
     4381 CCTACGTGAA ACCCGAGCAA CATACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4441 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4501 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4561 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4621 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4681 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4741 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4801 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4861 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4921 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4981 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5041 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5101 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5161 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5221 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5281 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5341 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5401 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5461 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5521 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5581 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5641 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5701 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5761 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5821 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5881 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5941 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6001 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6061 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6121 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6181 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6241 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6301 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6361 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6421 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6481 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6541 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6601 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6661 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6721 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6781 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6841 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6901 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6961 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7021 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7081 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7141 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7201 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7261 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7321 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7381 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7441 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7501 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7561 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7621 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7681 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7741 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7801 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7861 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7921 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7981 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8041 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8101 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8161 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8221 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//