LOCUS       RC218907                7947 bp    DNA     circular  SYN 
DEFINITION  HERC6 (Myc-DDK-tagged)-Human hect domain and RLD 6 (HERC6), transcript variant 1
ACCESSION   NM_017912
VERSION     NM_017912.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4104..4195 
                     /label="Myc-DDK"  
     misc_feature    4225..4810 
                     /label="PolyA signal"  
     misc_feature    4282..4301 
                     /label="Restriction Enzyme"  
     misc_feature    5086..5758 
                     /label="ColE1"  
     misc_feature    6188..6982 
                     /label="Kan/Neo"  
     misc_feature    7017..7319 
                     /label="SV40 ori"  
     misc_feature    7405..7882 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4086..4092 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4086 
                     /label="HERC6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACTTCTGT TGGGGCGCCG ACTCCAGGGA GCTGCAGCGC CGGAGGACGG 
     1081 CGGGCAGCCC CGGGGCTGAG CTACTGCAGG CGGCCAGCGG GGAGCGCCAC TCTCTGCTGC 
     1141 TGCTGACCAA CCACAGGGTC CTCTCGTGCG GAGACAACAG CAGGGGTCAG CTGGGCCGCA 
     1201 GGGGCGCGCA GCGCGGGGAG CTGCCAGAAC CAATTCAGGC ATTGGAAACC CTAATTGTTG 
     1261 ATCTCGTGAG CTGCGGGAAG GAGCACTCCC TGGCTGTGTG CCACAAAGGA AGGGTCTTCG 
     1321 CATGGGGAGC TGGTTCTGAA GGGCAGCTGG GGATTGGAGA ATTCAAGGAA ATAAGTTTCA 
     1381 CACCTAAGAA AATAATGACT CTGAATGATA TAAAAATAAT ACAAGTTTCC TGTGGACACT 
     1441 ACCACTCCCT GGCATTATCA AAAGATAGCC AAGTGTTTTC GTGGGGAAAG AACAGCCATG 
     1501 GGCAGCTGGG CTTGGGGAAG GAGTTCCCCT CCCAAGCCAG CCCGCAGAGG GTGAGGTCCC 
     1561 TGGAGGGGAT CCCACTGGCT CAGGTGGCTG CCGGAGGGGC TCACAGCTTT GCCCTGTCTC 
     1621 TCTGTGGGAC TTCGTTTGGC TGGGGAAGTA ACAGTGCCGG GCAGCTGGCC CTCAGTGGGC 
     1681 GTAATGTCCC AGTGCAAAGC AACAAGCCTC TCTCAGTCGG TGCACTGAAG AATCTAGGTG 
     1741 TGGTTTATAT CAGCTGTGGT GATGCACACA CTGCGGTGCT TACCCAGGAC GGGAAAGTGT 
     1801 TCACATTTGG AGACAATCGC TCTGGACAGC TGGGATACAG CCCCACTCCT GAGAAGAGAG 
     1861 GTCCACAACT TGTGGAAAGA ATTGATGGCC TAGTTTCGCA GATAGATTGT GGAAGTTATC 
     1921 ACACCCTGGC ATATGTGCAC ACCACTGGTC AGGTGGTATC TTTTGGTCAT GGACCAAGTG 
     1981 ACACAAGCAA GCCAACTCAT CCGGAGGCCC TGACAGAGAA CTTTGACATT AGCTGCCTGA 
     2041 TTTCTGCTGA AGACTTCGTG GATGTTCAAG TCAAACACAT TTTTGCTGGA ACATATGCCA 
     2101 ACTTTGTGAC AACTCATCAG GATACTAGTT CCACACGTGC TCCCGGGAAA ACCCTGCCAG 
     2161 AAATAAGCCG AATTAGCCAG TCCATGGCAG AAAAATGGAT AGCAGTGAAA AGAAGAAGTA 
     2221 CTGAACATGA AATGGCTAAA AGTGAAATTA GAATGATATT TTCATCTCCT GCTTGTCTGA 
     2281 CTGCAAGTTT TTTAAAGAAA AGAGGAACTG GAGAAACGAC TTCCATTGAT GTGGACTTAG 
     2341 AAATGGCAAG AGATACCTTC AAGAAGTTAA CAAAAAAGGA ATGGATTTCT TCCATGATAA 
     2401 CTACGTGTCT CGAGGATGAT CTGCTCAGAG CTCTTCCATG CCATTCTCCA CACCAAGAAG 
     2461 CTTTATCAGT TTTCCTCCTG CTCCCAGAAT GTCCTGTGAT GCATGATTCT AAGAACTGGA 
     2521 AGAACCTGGT GGTTCCATTT GCAAAGGCTG TGTGTGAAAT GAGTAAACAA TCTTTGCAAG 
     2581 TCCTAAAGAA GTGTTGGGCA TTTTTGCAAG AATCTTCTCT GAATCCGCTG ATCCAGATGC 
     2641 TTAAAGCAGC CATCATCTCT CAGCTGCTTC ATCAGACTAA AACCGAACAG GATCACTGTA 
     2701 ATGTTAAAGC TCTTTTAGGA ATGATGAAAG AACTGCATAA GGTAAACAAA GCTAACTGTC 
     2761 GACTACCAGA AAATACTTTC AACATAAATG AACTCTCCAA CTTATTAAAC TTTTATATAG 
     2821 ATAGAGGAAG ACAGCTCTTT CGGGATAACC ACCTGATACC TGCAGAAACC CCCAGTCCTG 
     2881 TTATTTTCAG TGATTTTCCA TTTATCTTTA ATTCGCTATC CAAAATTAAA TTATTGCAAG 
     2941 CTGATTCACA TATAAAGATG CAGATGTCAG AAAAGAAAGC ATACATGCTT ATGCATGAAA 
     3001 CAATTCTGCA AAAAAAGGAT GAATTTCCTC CATCACCCAG ATTTATACTT AGAGTCAGAC 
     3061 GAAGTCGCCT GGTTAAAGAT GCTCTGCGTC AATTAAGTCA AGCTGAAGCT ACTGACTTCT 
     3121 GCAAAGTATT AGTGGTTGAA TTTATTAATG AAATTTGTCC TGAGTCTGGA GGGGTTAGTT 
     3181 CAGAGTTCTT CCACTGTATG TTTGAAGAGA TGACCAAGCC AGAATATGGA ATGTTCATGT 
     3241 ATCCTGAAAT GGGTTCCTGC ATGTGGTTTC CTGCCAAGCC TAAACCTGAG AAGAAAAGAT 
     3301 ATTTCCTCTT TGGAATGCTG TGTGGACTCT CCTTATTCAA TTTAAATGTT GCTAACCTTC 
     3361 CTTTCCCACT GGCTCTGTAT AAAAAACTTC TGGACCAAAA GCCATCATTG GAAGATTTAA 
     3421 AAGAACTCAG TCCTCGGTTG GGGAAGAGTT TGCAAGAAGT TCTAGATGAT GCTGCTGATG 
     3481 ACATTGGAGA TGCGCTCTGC ATACGCTTTT CTATACACTG GGACCAAAAT GATGTTGACT 
     3541 TAATTCCAAA TGGGATCTCC ATACCTGTGG ACCAAACCAA CAAGAGAGAC TATGTTTCTA 
     3601 AGTATATTGA TTACATTTTC AACGTCTCTG TAAAAGCAGT TTATGAGGAA TTTCAGAGAG 
     3661 GATTTTATAG AGTCTGTGAG AAGGAGATAC TTAGACATTT CTACCCTGAA GAACTAATGA 
     3721 CAGCAATCAT TGGAAATACT GATTATGACT GGAAACAGTT TGAACAGAAT TCAAAGTATG 
     3781 AGCAAGGATA CCAAAAATCA CATCCTACTA TACAGTTGTT TTGGAAGGCT TTCCACAAAC 
     3841 TAACCTTGGA TGAAAAGAAA AAATTCCTCT TTTTCCTTAC AGGACGTGAT AGGCTGCATG 
     3901 CAAGAGGCAT ACAGAAAATG GAAATAGTAT TTCGCTGTCC TGAAACTTTC AGTGAAAGAG 
     3961 ATCACCCAAC ATCAATAACT TGTCATAATA TTCTCTCCCT CCCTAAGTAT TCTACAATGG 
     4021 AAAGAATGGA GGAAGCACTT CAAGTAGCCA TCAACAACAA CAGAGGATTT GTCTCACCCA 
     4081 TGCTCACACA GTCAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4141 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4201 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4261 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4321 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4381 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4441 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4501 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4561 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4621 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4681 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4741 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4801 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4861 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4921 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4981 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5041 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5101 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5161 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5221 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5281 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5341 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5401 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5461 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5521 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5581 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5641 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5701 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5761 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5821 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5881 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5941 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6001 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6061 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6121 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6181 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6241 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6301 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6361 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6421 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6481 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6541 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6601 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6661 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6721 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6781 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6841 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6901 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6961 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7021 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7081 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7141 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7201 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7261 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7321 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7381 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7441 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7501 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7561 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7621 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7681 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7741 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7801 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7861 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7921 ATTTAACAAA AATTTAACGC GAATTTT
//