LOCUS       RC218899                7713 bp    DNA     circular  SYN 
DEFINITION  GANAB (Myc-DDK-tagged)-Human glucosidase, alpha, neutral AB (GANAB), transcript variant 2
ACCESSION   NM_198334
VERSION     NM_198334.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3870..3961 
                     /label="Myc-DDK"  
     misc_feature    3991..4576 
                     /label="PolyA signal"  
     misc_feature    4048..4067 
                     /label="Restriction Enzyme"  
     misc_feature    4852..5524 
                     /label="ColE1"  
     misc_feature    5954..6748 
                     /label="Kan/Neo"  
     misc_feature    6783..7085 
                     /label="SV40 ori"  
     misc_feature    7171..7648 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3852..3858 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3852 
                     /label="GANAB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGTA GCGGCAGTGG CGGCGCGTAG GAGGCGGTCT TGGGCGTCTT 
     1081 TGGTACTGGC TTTTTTAGGG GTCTGCCTGG GGATTACCCT TGCTGTGGAT AGAAGCAACT 
     1141 TTAAGACCTG TGAAGAGAGT TCTTTCTGCA AGCGACAGAG AAGCATACGG CCAGGCCTCT 
     1201 CTCCATACCG AGCCTTGCTG GACTCTCTAC AGCTTGGTCC TGATTCCCTC ACGGTCCATC 
     1261 TGATCCATGA GGTCACCAAG GTGTTGCTGG TGCTAGAGCT TCAGGGGCTT CAAAAGAACA 
     1321 TGACTCGGTT CAGGATTGAT GAGCTGGAGC CTCGGCGACC CCGATACCGT GTACCAGATG 
     1381 TTTTGGTGGC TGATCCACCA ATAGCCCGGC TTTCTGTCTC TGGTCGTGAT GAGAACAGTG 
     1441 TGGAGTTAAC CATGGCTGAG GGACCCTACA AGATCATCTT GACAGCACGG CCATTCCGCC 
     1501 TTGACCTACT AGAGGACCGA AGTCTTTTGC TTAGTGTCAA TGCCCGAGGA CTCTTGGAGT 
     1561 TTGAGCATCA GAGGGCCCCT AGGGTCTCGC AAGGATCAAA AGACCCAGCT GAGGGCGATG 
     1621 GGGCCCAGCC TGAGGAAACA CCCAGGGATG GCGACAAGCC AGAGGAGACT CAGGGGAAGG 
     1681 CAGAGAAAGA TGAGCCAGGA GCCTGGGAGG AGACATTCAA AACTCACTCT GACAGCAAGC 
     1741 CGTATGGCCC CATGTCTGTG GGTTTGGACT TCTCTCTGCC AGGCATGGAG CATGTCTATG 
     1801 GGATCCCTGA GCATGCAGAC AACCTGAGGC TGAAGGTCAC TGAGGGTGGG GAGCCATATC 
     1861 GCCTCTACAA TTTGGATGTG TTCCAGTATG AGCTGTACAA CCCAATGGCC TTGTATGGGT 
     1921 CTGTGCCTGT GCTCCTGGCA CACAACCCTC ATCGCGACTT GGGCATCTTC TGGCTCAATG 
     1981 CTGCAGAGAC CTGGGTTGAT ATATCTTCCA ACACTGCCGG GAAGACCCTG TTTGGGAAGA 
     2041 TGATGGACTA CCTGCAGGGC TCTGGGGAGA CCCCACAGAC AGATGTTCGC TGGATGTCAG 
     2101 AGACTGGCAT CATTGACGTC TTCCTGCTGC TGGGGCCCTC CATCTCTGAT GTTTTCCGGC 
     2161 AATATGCTAG TCTCACAGGA ACCCAGGCGT TGCCCCCACT CTTCTCCCTC GGCTACCACC 
     2221 AGAGCCGTTG GAACTACCGG GACGAGGCTG ATGTGCTGGA AGTGGATCAG GGCTTTGATG 
     2281 ATCACAACCT GCCCTGTGAT GTCATCTGGC TAGACATTGA ACATGCTGAT GGCAAGCGGT 
     2341 ATTTCACCTG GGACCCCAGT CGCTTCCCTC AGCCCCGCAC CATGCTTGAG CGCTTGGCTT 
     2401 CTAAGAGGCG GAAGCTGGTG GCCATCGTAG ACCCCCACAT CAAGGTGGAC TCCGGCTACC 
     2461 GAGTTCACGA GGAGCTGCGG AACCTGGGGC TGTATGTTAA AACCCGGGAT GGCTCTGACT 
     2521 ATGAGGGCTG GTGCTGGCCA GGCTCAGCTG GTTACCCTGA CTTCACTAAT CCCACGATGA 
     2581 GGGCCTGGTG GGCTAACATG TTCAGCTATG ACAATTATGA GGGCTCAGCT CCCAACCTCT 
     2641 TTGTCTGGAA TGACATGAAC GAACCATCTG TGTTCAATGG TCCTGAGGTC ACCATGCTCA 
     2701 AGGATGCCCA GCATTATGGG GGCTGGGAGC ACCGGGATGT GCATAACATC TATGGCCTTT 
     2761 ATGTGCACAT GGCGACTGCT GATGGGCTGA GACAGCGCTC TGGGGGCATG GAACGCCCCT 
     2821 TTGTCCTGGC CAGGGCCTTC TTCGCTGGCT CCCAGCGCTT TGGAGCCGTG TGGACAGGGG 
     2881 ACAACACTGC CGAGTGGGAC CATTTGAAGA TCTCTATTCC TATGTGTCTC AGCTTGGGGC 
     2941 TGGTGGGACT TTCCTTCTGT GGGGCGGATG TGGGTGGCTT CTTCAAAAAC CCAGAGCCAG 
     3001 AGCTGCTTGT GCGCTGGTAC CAGATGGGTG CTTACCAGCC ATTCTTCCGG GCACATGCCC 
     3061 ACTTGGACAC TGGGCGACGA GAGCCATGGC TGTTACCATC TCAGCACAAT GATATAATCC 
     3121 GAGATGCCTT GGGCCAGCGA TATTCTTTGC TGCCCTTCTG GTACACCCTC TTATATCAGG 
     3181 CCCATCGGGA AGGCATTCCT GTCATGAGGC CCCTGTGGGT GCAGTACCCT CAGGATGTGA 
     3241 CTACCTTCAA TATAGATGAT CAGTACTTGC TTGGGGATGC GTTGCTGGTT CACCCTGTAT 
     3301 CAGACTCTGG AGCCCATGGT GTCCAGGTCT ATCTGCCTGG CCAAGGGGAG GTGTGGTATG 
     3361 ACATTCAAAG CTACCAGAAG CATCATGGTC CCCAGACCCT GTACCTGCCT GTAACTCTAA 
     3421 GCAGTATCCC TGTGTTCCAG CGTGGAGGGA CAATCGTGCC TCGATGGATG CGAGTGCGGC 
     3481 GGTCTTCAGA ATGTATGAAG GATGACCCCA TCACTCTCTT TGTTGCACTT AGCCCTCAGG 
     3541 GTACAGCTCA AGGAGAGCTC TTTCTGGATG ATGGGCACAC GTTCAACTAT CAGACTCGCC 
     3601 AAGAGTTCCT GCTGCGTCGA TTCTCATTCT CTGGCAACAC CCTTGTCTCC AGCTCAGCAG 
     3661 ACCCTGAAGG ACACTTTGAG ACACCAATCT GGATTGAGCG GGTGGTGATA ATAGGGGCTG 
     3721 GAAAGCCAGC AGCTGTGGTA CTCCAGACAA AAGGATCTCC AGAAAGCCGC CTGTCCTTCC 
     3781 AGCATGACCC TGAGACCTCT GTGTTGGTCC TGCGCAAGCC TGGCATCAAT GTGGCATCTG 
     3841 ATTGGAGTAT TCACCTGCGA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3901 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3961 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4021 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4081 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4141 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4201 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4261 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4321 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4381 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4441 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4501 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4561 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4621 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4681 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4741 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4801 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4861 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4921 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4981 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5041 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5101 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5161 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5221 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5281 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5341 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5401 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5461 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5521 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5581 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5641 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5701 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5761 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5821 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5881 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5941 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6001 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6061 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6121 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6181 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6241 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6301 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6361 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6421 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6481 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6541 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6601 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6661 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6721 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6781 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6841 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6901 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6961 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7021 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7081 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7141 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7201 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7261 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7321 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7381 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7441 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7501 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7561 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7621 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7681 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//