LOCUS       RC218888                7740 bp    DNA     circular  SYN 
DEFINITION  PCDHA8 (Myc-DDK-tagged)-Human protocadherin alpha 8 (PCDHA8), transcript variant 1
ACCESSION   NM_018911
VERSION     NM_018911.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3895..3986 
                     /label="Myc-DDK"  
     misc_feature    4016..4601 
                     /label="PolyA signal"  
     misc_feature    4073..4092 
                     /label="Restriction Enzyme"  
     misc_feature    4877..5549 
                     /label="ColE1"  
     misc_feature    5979..6773 
                     /label="Kan/Neo"  
     misc_feature    6808..7110 
                     /label="SV40 ori"  
     misc_feature    7196..7673 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3870..3877 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3870 
                     /label="PCDHA8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTATCAC TGGCGAGGAG AGCTGGGATC CTGGCGACTA CTACTCTTGC 
     1081 TTCTGCTCCT CGCAGCCTGG AAGGTGGGGA GCGGCCAGCT CCACTACTCC GTCCCCGAGG 
     1141 AGGCCAAACA CGGCACCTTC GTGGGCCGGA TCGCGCAGGA CCTGGGGCTG GAGCTGGCGG 
     1201 AGCTGGTGCC GCGCCTGTTC CGGGTGGCGT CCAAAAGACA CCGGGACCTT CTGGAGGTAA 
     1261 GTCTGCAGAA TGGCATTTTG TTTGTGAATT CTCGGATCGA CCGCGAGGAG CTGTGCGGGC 
     1321 GGAGCGCGGA GTGCAGCATC CACCTGGAGG TGATCGTGGA CAGGCCGCTG CAGGTTTTCC 
     1381 ATGTGGACGT GGAGGTGAAG GATGTTAATG ACAACCCGCC AGTGTTCCGG GTAAAAGACC 
     1441 AAAAGCTGTT TGTTTCAGAA TCCAGAATGC CAGACTCTCG GTTTCCGCTA GAGGGCGCGT 
     1501 CCGATGCAGA TGTTGGAGCT AACTCCGTGT TAACCTACAG GCTTAGCTCT CATGATTACT 
     1561 TCATGCTAGA TGTGAATTCA AAGAACGATG AGAATAAACT GGTTGAGCTC GTATTAAGAA 
     1621 AATCCTTGGA CAGAGAGGAC GCTCCTGCGC ACCACTTATT CCTGACAGCC ACAGATGGGG 
     1681 GCAAACCTGA GCTCACAGGC ACTGTTCAGC TGCTGGTCAC AGTGCTGGAT GTGAATGATA 
     1741 ATGCTCCCAC TTTCGAACAG TCTGAATACG AAGTAAGAAT ATTCGAAAAC GCAGACAACG 
     1801 GAACAACAGT TATCAAACTG AATGCTTCTG ATCCGGATGA AGGAGCCAAT GGGGCAATTT 
     1861 CATATTCTTT TAATAGCCTT GTTGAAACTA TGGTTATTGA CCACTTTAGC ATAGATCGAA 
     1921 ATACGGGAGA AATAGTGATT CGGGGTAATT TGGATTTTGA ACAAGAAAAC TTATACAAAA 
     1981 TCCTCATTGA CGCCACGGAC AAAGGCCATC CTCCCATGGC GGGTCATTGC ACCGTTTTAG 
     2041 TGAGAATTTT GGATAAAAAT GATAACGTCC CTGAGATAGC ACTGACTTCC TTATCCTTGC 
     2101 CTGTACGTGA AGACGCTCAA TTTGGTACTG TCATCGCCCT AATTAGCGTG AACGACCTCG 
     2161 ATTCAGGTGC CAACGGGCAG GTGACCTGCT CCCTGATGCC CCATGTCCCC TTCAAGCTGG 
     2221 TGTCCACCTT CAAGAATTAC TACTCGTTGG TGCTGGACAG CGCCCTGGAC CGCGAGAGAG 
     2281 TGTCGGCCTA TGAGTTGGTG GTAACCGCGC GGGACGGGGG CTCGCCTTCG CTGTGGGCCA 
     2341 CCGCCAGCTT GTCTGTGGAG GTGGCCGACG TGAACGACAA TGCTCCGGCG TTCGCGCAGC 
     2401 CCGAGTACAC GGTGTTCGTG AAGGAGAACA ACCCGCCGGG CTGCCACATC TTCACGGTGT 
     2461 CTGCGCGAGA CGCGGACGCG CAGGAGAACG CGCTGGTGTC CTACTCGCTT GTGGAGCGGC 
     2521 GGGTGGGCGA GCGCTCGCTG TCGAGCTACA TTTCGGTGCA CACGGAGAGC GGCAAGGTGT 
     2581 ACGCGCTGCA GCCGCTGGAC CACGAGGAGC TAGAGCTGCT GCAGTTCCAG GTGAGCGCGC 
     2641 GCGACGCGGG CGTGCCGCCT CTGGGCAGCA ACGTGACGCT GCAGGTGTTC GTGCTGGACG 
     2701 AGAATGACAA CGCGCCGGCA CTGCTGGAGC CTCGGGTGGG TGGCACTGGT GGCGCAGCGA 
     2761 GCAAGCTGGT GCCGCGGTCT GTGGGCGCGG GCCACGTGGT AGCGAAGGTG CGCGCAGTGG 
     2821 ACGCCGACTC GGGCTACAAC GCGTGGCTTT CGTATGAGCT GCAGCCAGCT GCAAGCAGCC 
     2881 CTCGCATCCC GTTCCGCGTG GGGCTGTACA CGGGCGAGAT CAGCACCACT CGTGTCCTGG 
     2941 ACGAAGCGGA CTCTCCGCGC CACCGTCTGC TGGTCCTGGT GAAGGATCAT GGTGAACCTG 
     3001 CGCTGACCGC CACGGCCACG GTTCTGGTGT CGCTGGTGGA GAGCGGCCAG GCTCCAAAAG 
     3061 CGTCATCGAG GCAGTCGGCT GGCGTTTTGG GTCCGGAAGC GGCGCTGGTG GATGTCAACG 
     3121 TGTACCTGAT CATCGCCATC TGCGCGGTAT CCAGCCTGCT GGTGCTCACG CTGCTGCTGT 
     3181 ACACTGCGCT GCGGTGCTCA GCACTGCCCA CTGAGGGCGG GTGCCGGGCG GGCAAGCCCA 
     3241 CTCTGGTGTG CTCCAGTGCG GTGGGGAGCT GGTCATACTC GCAACAACAG CCGCAGAGGG 
     3301 TGTGCTCTGG TGAGGGGCCA CCGAAGACGG ACCTCATGGC CTTCAGCCCC TGCCTTCCTC 
     3361 CTGATCTGGG ATCAGTTGAT GTAGGCGAAG AGCAAGATTT AAATGTTGAT CATGGCCTCA 
     3421 AACCACGACA GCCCAACCCT GACTGGCGTT ACTCTGCCTC CCTGAGAGCA GGCATGCACA 
     3481 GCTCTGTGCA CCTAGAGGAG GCTGGCATTC TACGGGCTGG TCCAGGAGGG CCTGATCAGC 
     3541 AGTGGCCAAC AGTATCCAGT GCAACACCAG AACCAGAGGC AGGAGAAGTG TCCCCTCCAG 
     3601 TCGGTGCGGG TGTCAACAGC AACAGCTGGA CCTTTAAATA CGGACCAGGC AACCCCAAAC 
     3661 AATCCGGTCC CGGTGAGTTG CCCGACAAAT TCATTATCCC AGGATCTCCT GCAATCATCT 
     3721 CCATCCGGCA GGAGCCTACT AACAGCCAAA TTGACAAAAG TGACTTCATA ACCTTCGGCA 
     3781 AAAAGGAGGA GACCAAGAAA AAGAAGAAAA AGAAGAAGGG TAACAAGACC CAGGAGAAAA 
     3841 AAGAGAAAGG GAACAGCACG ACTGACAACA GTGACCAGAG CGGACCGACG CGTACGCGGC 
     3901 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3961 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4021 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4081 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4141 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4201 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4261 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4321 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4381 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4441 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4501 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4561 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4621 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4681 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4741 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4801 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4861 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4921 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4981 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5041 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5101 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5161 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5221 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5281 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5341 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5401 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5461 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5521 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5581 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5641 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5701 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5761 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5821 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5881 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5941 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6001 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6061 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6121 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6181 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6241 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6301 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6361 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6421 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6481 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6541 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6601 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6661 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6721 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6781 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6841 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6901 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6961 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7021 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7081 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7141 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7201 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7261 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7321 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7381 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7441 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7501 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7561 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7621 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7681 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//