LOCUS       RC218866                8622 bp    DNA     circular  SYN 
DEFINITION  SASH1 (Myc-DDK-tagged)-Human SAM and SH3 domain containing 1 (SASH1)
ACCESSION   NM_015278
VERSION     NM_015278.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4779..4870 
                     /label="Myc-DDK"  
     misc_feature    4900..5485 
                     /label="PolyA signal"  
     misc_feature    4957..4976 
                     /label="Restriction Enzyme"  
     misc_feature    5761..6433 
                     /label="ColE1"  
     misc_feature    6863..7657 
                     /label="Kan/Neo"  
     misc_feature    7692..7994 
                     /label="SV40 ori"  
     misc_feature    8080..8557 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4761..4767 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4761 
                     /label="SASH1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGACGCG GGAGCAGCTG GCCCGGGGCC GGAGCCTGAG CCCGAGCCCG 
     1081 AGCCGGAGCC CGAGCCCGCG CCGGAGCCGG AACCGGAGCC CAAGCCGGGT GCTGGCACAT 
     1141 CCGAGGCGTT CTCCCGACTC TGGACCGACG TGATGGGTAT CCTGGACGGT TCACTGGGAA 
     1201 ACATCGATGA CCTGGCGCAG CAGTATGCAG ATTATTACAA CACCTGTTTC TCCGACGTGT 
     1261 GCGAGAGGAT GGAGGAGCTG CGGAAACGGC GGGTTTCCCA GGACCTGGAA GTGGAGAAAC 
     1321 CCGATGCTAG CCCCACGTCA CTTCAGCTGC GGTCCCAGAT CGAAGAGTCG CTTGGCTTCT 
     1381 GTAGCGCCGT GTCAACCCCA GAAGTGGAAA GAAAGAACCC TCTTCATAAA TCAAACTCAG 
     1441 AAGACAGCTC TGTAGGAAAA GGAGACTGGA AGAAGAAAAA TAAGTATTTC TGGCAGAACT 
     1501 TCCGAAAGAA CCAGAAAGGA ATAATGAGAC AGACTTCAAA AGGAGAAGAC GTTGGTTATG 
     1561 TTGCCAGTGA AATAACGATG AGCGATGAGG AGCGGATTCA GCTAATGATG ATGGTCAAAG 
     1621 AAAAGATGAT CACAATTGAG GAAGCACTTG CTAGGCTCAA GGAATACGAG GCCCAGCACC 
     1681 GGCAGTCGGC TGCCCTGGAC CCTGCTGACT GGCCAGATGG TTCTTACCCA ACGTTTGATG 
     1741 GCTCATCAAA CTGCAATTCA AGAGAACAAT CGGATGATGA GACTGAGGAG TCGGTGAAGT 
     1801 TTAAGAGGTT ACACAAGCTG GTAAACTCCA CTCGCAGAGT CAGAAAGAAA CTAATTAGGG 
     1861 TGGAAGAAAT GAAAAAACCC AGCACTGAAG GTGGGGAGGA GCACGTGTTT GAGAATTCGC 
     1921 CGGTCCTGGA TGAACGGTCC GCCCTCTACT CTGGCGTGCA CAAGAAGCCC CTTTTCTTTG 
     1981 ATGGCTCTCC TGAGAAACCT CCCGAAGATG ACTCAGACTC TCTCACCACG TCTCCATCCT 
     2041 CCAGCAGCCT GGACACCTGG GGGGCTGGCC GGAAGTTGGT CAAAACCTTC AGCAAAGGAG 
     2101 AGAGCCGGGG CCTGATTAAG CCCCCCAAGA AGATGGGGAC ATTCTTCTCC TACCCAGAAG 
     2161 AAGAAAAGGC CCAGAAAGTG TCCCGCTCCC TCACCGAGGG GGAGATGAAG AAGGGTCTCG 
     2221 GGTCCCTAAG CCACGGGAGA ACCTGCAGTT TTGGAGGATT TGACTTGACG AATCGCTCTC 
     2281 TGCACGTTGG CAGTAATAAT TCTGACCCAA TGGGTAAAGA AGGAGACTTT GTGTACAAAG 
     2341 AAGTCATCAA ATCACCTACT GCCTCTCGCA TCTCTCTTGG GAAAAAGGTG AAATCAGTGA 
     2401 AAGAGACGAT GAGAAAGAGA ATGTCTAAAA AATACAGCAG CTCTGTCTCT GAGCAGGACT 
     2461 CGGGCCTTGA TGGAATGCCT GGCTCCCCTC CGCCTTCACA GCCCGACCCC GAACACTTGG 
     2521 ACAAGCCCAA GCTCAAGGCC GGGGGTTCTG TAGAAAGTCT TCGCAGTTCT CTCAGTGGGC 
     2581 AGAGCTCCAT GAGCGGTCAA ACAGTGAGCA CCACTGATTC CTCAACCAGC AACCGGGAAA 
     2641 GCGTCAAGTC GGAAGATGGG GATGACGAAG AGCCGCCTTA CCGAGGCCCG TTCTGCGGGC 
     2701 GTGCCAGGGT GCACACCGAC TTCACCCCCA GTCCCTATGA CACAGACTCA CTCAAGCTCA 
     2761 AGAAAGGAGA TATCATCGAT ATAATCAGCA AGCCACCCAT GGGGACCTGG ATGGGCCTGC 
     2821 TGAACAACAA AGTCGGCACG TTCAAGTTCA TCTACGTGGA CGTGCTCAGT GAAGACGAGG 
     2881 AGAAACCCAA ACGCCCCACC AGGAGGCGTC GGAAAGGACG ACCACCCCAG CCCAAGTCTG 
     2941 TGGAGGATCT CCTGGATCGG ATTAACCTAA AAGAGCACAT GCCCACTTTC CTGTTCAATG 
     3001 GATATGAAGA TTTGGACACC TTTAAGCTGC TGGAGGAGGA AGACTTGGAT GAGTTAAATA 
     3061 TCAGGGACCC GGAACACAGA GCTGTTCTCT TGACAGCAGT GGAGCTGTTA CAAGAGTATG 
     3121 ACAGTAACAG CGACCAGTCA GGATCCCAGG AGAAGCTGCT CGTTGACAGC CAGGCCCTGA 
     3181 GTGGATGCTC ACCCCGAGAC TCAGGATGCT ACGAAAGCAG TGAGAACCTG GAAAACGGCA 
     3241 AGACTCGGAA AGCTAGCCTC CTATCTGCCA AGTCATCCAC CGAGCCCAGC TTGAAGTCTT 
     3301 TTAGCAGAAA CCAGTTGGGC AATTACCCAA CATTGCCTTT AATGAAATCA GGGGATGCAC 
     3361 TGAAGCAGGG ACAGGAGGAG GGCAGGCTGG GTGGTGGCCT TGCCCCAGAC ACGTCCAAGA 
     3421 GCTGTGACCC ACCTGGTGTG ACTGGTTTGA ATAAAAACCG AAGAAGCCTC CCAGTTTCCA 
     3481 TCTGCCGGAG CTGTGAGACC CTGGAGGGCC CCCAGACTGT GGACACTTGG CCCCGATCCC 
     3541 ATTCCCTGGA TGACCTTCAA GTGGAGCCTG GTGCTGAGCA AGACGTGCCT ACCGAGGTGA 
     3601 CAGAACCGCC CCCTCAGATT GTACCTGAAG TGCCACAGAA GACGACCGCC TCTTCCACGA 
     3661 AGGCCCAGCC CCTGGAGCAA GACTCTGCTG TCGACAATGC ATTGCTACTG ACCCAAAGCA 
     3721 AGAGATTTTC TGAACCTCAG AAATTGACAA CTAAGAAACT GGAGGGCTCA ATCGCAGCCT 
     3781 CTGGTCGCGG CCTGTCACCC CCTCAGTGTT TGCCCAGAAA CTATGATGCT CAGCCTCCTG 
     3841 GAGCTAAACA CGGTTTAGCA AGGACGCCTC TGGAGGGCCA CAGAAAAGGA CACGAGTTTG 
     3901 AAGGAACACA CCATCCCCTG GGCACCAAAG AAGGGGTAGA TGCTGAGCAG AGAATGCAGC 
     3961 CCAAAATTCC ATCACAGCCT CCACCTGTTC CTGCCAAAAA GAGCAGAGAA CGCCTTGCTA 
     4021 ACGGACTCCA CCCTGTTCCC ATGGGCCCCA GTGGGGCCCT CCCCAGTCCC GATGCGCCAT 
     4081 GCCTGCCAGT GAAAAGGGGC AGCCCCGCCA GCCCCACCAG CCCTAGCGAC TGTCCCCCAG 
     4141 CACTGGCTCC CAGGCCTCTC TCAGGGCAGG CGCCTGGCAG CCCACCAAGC ACAAGGCCGC 
     4201 CCCCCTGGCT CTCAGAGCTC CCCGAGAACA CAAGCCTCCA GGAGCACGGT GTGAAGCTGG 
     4261 GCCCGGCTTT GACCAGGAAG GTCTCCTGTG CCCGGGGAGT GGATCTAGAA ACGCTCACTG 
     4321 AAAACAAGCT GCACGCTGAA GGCATCGATC TCACGGAGGA GCCGTATTCT GATAAGCATG 
     4381 GCCGCTGTGG GATTCCTGAA GCCCTGGTGC AGAGATACGC AGAGGACTTG GATCAGCCCG 
     4441 AGCGGGACGT CGCCGCCAAC ATGGACCAGA TCCGGGTGAA GCAGCTTCGG AAGCAGCACC 
     4501 GCATGGCGAT TCCAAGTGGT GGACTCACGG AAATCTGCCG AAAGCCCGTC TCTCCTGGGT 
     4561 GCATTTCGTC TGTGTCAGAT TGGCTCATTT CCATCGGTCT GCCCATGTAC GCCGGCACCC 
     4621 TCTCCACCGC GGGCTTCAGC ACACTGAGCC AAGTGCCTTC TCTGTCTCAC ACTTGCCTTC 
     4681 AGGAGGCCGG CATCACAGAG GAGAGACACA TAAGAAAGCT CCTATCTGCA GCCAGACTCT 
     4741 TCAAACTGCC GCCAGGCCCT GAGGCCATGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4801 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4861 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4921 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4981 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5041 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5101 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5161 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5221 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5281 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5341 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5401 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5461 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5521 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5581 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5641 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5701 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5761 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5821 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5881 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5941 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6001 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6061 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6121 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6181 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6241 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6301 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6361 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6421 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6481 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6541 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6601 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6661 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6721 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6781 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6841 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6901 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6961 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7021 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7081 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7141 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7201 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7261 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7321 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7381 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7441 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7501 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7561 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7621 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7681 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7741 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7801 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7861 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7921 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8041 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8101 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8161 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8221 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8281 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8341 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8401 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8461 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8521 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8581 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//