LOCUS       RC218855                9189 bp    DNA     circular  SYN 
DEFINITION  NUP160 (Myc-DDK-tagged)-Human nucleoporin 160kDa (NUP160)
ACCESSION   NM_015231
VERSION     NM_015231.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5346..5437 
                     /label="Myc-DDK"  
     misc_feature    5467..6052 
                     /label="PolyA signal"  
     misc_feature    5524..5543 
                     /label="Restriction Enzyme"  
     misc_feature    6328..7000 
                     /label="ColE1"  
     misc_feature    7430..8224 
                     /label="Kan/Neo"  
     misc_feature    8259..8561 
                     /label="SV40 ori"  
     misc_feature    8647..9124 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5328..5334 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5328 
                     /label="NUP160"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTTCACCTG TCCGCAGCTC CGCCCGCCCC ACCCCCGGAA GTGACGGCGA 
     1081 CCGCGCGGCC CTGCCTTTGT TCCGTTGGGC GTCGCGGCGA CGGCGGGAAG ATGGCGGCGG 
     1141 CGGGAGCCCT GGAACGGAGC TTCGTGGAGC TAAGCGGAGC TGAGCGCGAA AGGCCGAGGC 
     1201 ACTTTCGGGA ATTCACAGTC TGCAGCATTG GGACTGCAAA TGCCGTGGCT GGCGCCGTAA 
     1261 AATACAGTGA AAGCGCGGGA GGCTTTTACT ACGTGGAGAG TGGCAAGTTG TTCTCCGTAA 
     1321 CCAGAAACAG GTTCATTCAT TGGAAGACCT CTGGAGATAC ATTGGAGCTG ATGGAGGAGT 
     1381 CACTGGACAT AAATCTGTTG AATAATGCCA TTCGCCTAAA ATTCCAAAAT TGCAGTGTTT 
     1441 TACCTGGAGG GGTTTATGTC TCTGAGACTC AGAATCGTGT GATAATCTTG ATGTTAACCA 
     1501 ATCAAACAGT GCACAGGTTA CTTTTACCAC ACCCCTCCCG GATGTATAGG AGTGAGTTGG 
     1561 TAGTTGACAG TCAGATGCAG TCAATATTCA CTGACATTGG AAAAGTTGAT TTCACAGATC 
     1621 CTTGCAACTA TCAGTTAATT CCAGCAGTAC CTGGAATATC TCCTAATTCC ACCGCCTCTA 
     1681 CAGCCTGGCT CAGCAGTGAT GGGGAGGCCC TGTTTGCCTT ACCATGTGCT TCTGGGGGAA 
     1741 TCTTTGTTCT TAAGCTACCT CCTTATGACA TACCTGGTAT GGTGTCAGTC GTGGAACTGA 
     1801 AACAGAGTTC AGTAATGCAA CGATTGCTTA CAGGCTGGAT GCCAACAGCT ATCAGGGGTG 
     1861 ACCAGTCGCC TTCAGATCGT CCCCTCAGTC TTGCTGTTCA TTGTGTGGAG CATGATGCCT 
     1921 TCATCTTTGC TTTGTGTCAG GATCATAAAC TACGAATGTG GTCTTACAAG GAGCAAATGT 
     1981 GCCTAATGGT AGCTGACATG CTGGAGTATG TCCCTGTGAA GAAAGACCTT CGGCTTACTG 
     2041 CTGGAACTGG ACACAAATTA CGGCTTGCTT ATTCCCCCGC CATGGGACTC TACCTGGGGA 
     2101 TATACATGCA TGCACCAAAA CGAGGACAGT TCTGCATTTT CCAGTTGGTG AGCACTGAGA 
     2161 GTAATCGCTA TAGTCTCGAT CATATTTCTT CACTGTTCAC TTCTCAGGAG ACACTGATTG 
     2221 ACTTTGCCTT AACTTCCACG GATATCTGGG CCCTGTGGCA TGATGCTGAG AACCAAACAG 
     2281 TAGTGAAATA CATCAACTTT GAACATAATG TTGCAGGTCA GTGGAATCCA GTTTTTATGC 
     2341 AGCCTCTGCC AGAGGAAGAG ATTGTCATCA GAGATGATCA AGACCCCAGA GAGATGTATC 
     2401 TGCAAAGTCT TTTTACACCA GGACAATTCA CAAATGAAGC TTTATGTAAG GCTTTACAGA 
     2461 TTTTCTGCCG AGGAACTGAG AGGAATTTGG ATCTTTCCTG GAGTGAACTG AAGAAAGAAG 
     2521 TTACTTTAGC TGTTGAAAAT GAGCTTCAAG GAAGTGTAAC AGAGTATGAA TTCTCCCAGG 
     2581 AGGAGTTTCG AAATTTACAA CAAGAATTCT GGTGCAAGTT CTATGCCTGT TGTCTTCAGT 
     2641 ATCAAGAAGC CCTCTCTCAC CCTCTTGCCC TACATTTGAA TCCACACACA AACATGGTGT 
     2701 GCCTGCTGAA AAAAGGGTAC CTGTCTTTCC TTATTCCCTC ATCCTTAGTG GATCATTTGT 
     2761 ATCTCCTGCC TTATGAGAAC CTTTTGACAG AAGATGAGAC AACCATATCT GATGATGTGG 
     2821 ATATCGCTCG GGATGTCATA TGTCTTATAA AATGCCTCCG GCTGATTGAA GAGTCAGTAA 
     2881 CTGTGGATAT GTCAGTTATA ATGGAAATGA GTTGTTATAA CCTACAGTCT CCGGAAAAGG 
     2941 CTGCAGAGCA GATTCTGGAA GATATGATCA CTATTGATGT AGAAAATGTG ATGGAGGATA 
     3001 TTTGTAGTAA ACTGCAAGAG ATTAGGAACC CAATCCATGC AATTGGACTA CTTATACGGG 
     3061 AAATGGATTA TGAAACAGAA GTGGAAATGG AAAAGGGATT CAATCCAGCT CAGCCTTTGA 
     3121 ATATTCGAAT GAATCTTACC CAGCTCTATG GTAGTAACAC AGCAGGGTAT ATTGTGTGCA 
     3181 GAGGGGTGCA TAAAATCGCC AGTACTCGTT TCCTGATCTG CAGAGATCTT TTGATCTTAC 
     3241 AGCAGCTGTT AATGAGGCTT GGAGATGCTG TGATTTGGGG AACTGGTCAG CTCTTTCAAG 
     3301 CTCAGCAAGA CCTACTACAT CGAACAGCTC CCCTACTCTT ATCTTATTAC CTCATTAAAT 
     3361 GGGGAAGTGA GTGCTTGGCA ACTGATGTTC CACTTGACAC ACTGGAGTCT AATCTCCAAC 
     3421 ACTTATCAGT ACTGGAATTA ACAGACTCTG GTGCTTTAAT GGCAAATAGG TTTGTATCTA 
     3481 GTCCTCAGAC TATTGTGGAG TTATTCTTCC AAGAAGTTGC AAGAAAACAC ATTATATCTC 
     3541 ACCTCTTCTC TCAGCCAAAG GCACCTCTGA GCCAAACTGG ATTGAATTGG CCTGAAATGA 
     3601 TTACTGCAAT TACCAGTTAT TTATTGCAGC TTTTATGGCC TAGCAATCCT GGTTGTCTCT 
     3661 TTCTAGAATG TTTGATGGGA AATTGCCAAT ATGTACAATT GCAGGATTAT ATTCAACTGC 
     3721 TACATCCCTG GTGTCAAGTC AATGTTGGTT CCTGTCGATT TATGCTGGGA AGGTGTTACC 
     3781 TAGTTACAGG AGAAGGACAG AAGGCTCTGG AATGTTTTTG TCAGGCAGCA TCTGAAGTAG 
     3841 GCAAAGAGGA ATTCTTGGAT CGCTTGATTC GCTCAGAGGA TGGGGAGATC GTGTCTACCC 
     3901 CCAGGCTGCA GTATTATGAC AAGGTTTTAC GACTACTAGA TGTCATTGGT TTGCCTGAAC 
     3961 TGGTTATTCA GTTGGCTACA TCAGCCATAA CTGAAGCAGG TGATGACTGG AAAAGTCAGG 
     4021 CTACTCTAAG GACATGTATT TTCAAACATC ATTTGGATTT GGGTCACAAT AGCCAAGCAT 
     4081 ATGAAGCCTT AACCCAAATT CCTGATTCCA GCAGGCAATT AGATTGTTTA CGGCAGTTGG 
     4141 TGGTAGTTCT TTGTGAACGC TCACAGCTAC AGGATCTTGT AGAGTTTCCC TATGTGAATC 
     4201 TGCATAATGA GGTTGTGGGA ATAATTGAGT CACGTGCTAG AGCTGTGGAC CTTATGACTC 
     4261 ACAATTACTA TGAACTTCTG TATGCCTTTC ACATCTATCG CCACAATTAC CGCAAGGCTG 
     4321 GCACAGTGAT GTTTGAGTAT GGAATGCGGC TTGGCAGAGA AGTTCGAACT CTCCGGGGAC 
     4381 TTGAGAAACA AGGCAACTGT TATCTGGCTG CTCTCAATTG TTTACGACTT ATTCGTCCAG 
     4441 AATATGCGTG GATTGTGCAG CCAGTGTCTG GTGCAGTGTA TGATCGCCCT GGAGCATCCC 
     4501 CTAAGAGGAA TCATGATGGA GAATGCACAG CTGCCCCCAC AAATCGACAA ATTGAAATCC 
     4561 TGGAACTGGA AGATCTGGAG AAAGAGTGTT CCTTGGCTCG CATCCGCCTC ACTTTGGCTC 
     4621 AGCATGATCC ATCAGCGGTT GCAGTTGCTG GAAGTTCATC AGCAGAGGAA ATGGTCACTC 
     4681 TCTTGGTTCA GGCGGGCCTC TTTGACACTG CCATATCACT CTGTCAGACT TTTAAGCTTC 
     4741 CCTTAACGCC AGTCTTTGAA GGGCTTGCCT TCAAATGCAT CAAATTGCAA TTTGGAGGAG 
     4801 AGGCAGCACA AGCAGAAGCC TGGGCCTGGC TAGCAGCCAA TCAGCTCTCA TCTGTCATCA 
     4861 CTACTAAGGA GTCTAGTGCT ACAGATGAAG CATGGCGACT ATTATCCACT TACCTGGAGA 
     4921 GGTACAAAGT CCAGAATAAC TTGTATCACC ACTGTGTAAT CAACAAGCTC TTGTCTCATG 
     4981 GAGTGCCTCT GCCTAATTGG CTTATAAACA GTTACAAGAA GGTTGATGCT GCTGAATTGC 
     5041 TTCGTTTATA CTTAAACTAT GACCTTTTAG AAGAAGCTGT GGATTTGGTG TCAGAATATG 
     5101 TGGATGCTGT ATTGGGAAAA GGACATCAAT ACTTCGGAAT TGAGTTTCCA CTGTCCGCAA 
     5161 CAGCCCCAAT GGTGTGGCTT CCATACTCCT CTATTGATCA GCTTCTCCAA GCTCTGGGAG 
     5221 AGAACAGTGC CAACAGTCAC AACATCGCAC TGTCCCAGAA AATACTTGAC AAATTGGAGG 
     5281 ACTACCAGCA AAAAGTTGAT AAGGCAACAC GGGATTTATT ATATCGTCGG ACCTTGACGC 
     5341 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5401 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5461 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5521 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5581 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5641 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5701 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5761 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5821 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5881 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5941 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     6001 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     6061 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6121 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6181 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6241 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6301 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6361 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6421 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6481 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6541 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6601 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6661 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6721 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6781 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6841 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6901 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6961 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     7021 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     7081 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7141 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7201 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7261 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7321 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7381 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7441 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7501 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7561 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7621 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7681 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7741 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7801 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7861 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7921 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7981 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     8041 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     8101 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8161 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8221 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8281 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8341 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8401 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8461 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8521 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8581 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8641 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8701 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8761 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8821 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8881 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8941 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     9001 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     9061 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9121 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9181 GCGAATTTT
//