LOCUS       RC218842                8049 bp    DNA     circular  SYN 
DEFINITION  KIF11 (Myc-DDK-tagged)-Human kinesin family member 11 (KIF11)
ACCESSION   NM_004523
VERSION     NM_004523.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4206..4297 
                     /label="Myc-DDK"  
     misc_feature    4327..4912 
                     /label="PolyA signal"  
     misc_feature    4384..4403 
                     /label="Restriction Enzyme"  
     misc_feature    5188..5860 
                     /label="ColE1"  
     misc_feature    6290..7084 
                     /label="Kan/Neo"  
     misc_feature    7119..7421 
                     /label="SV40 ori"  
     misc_feature    7507..7984 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4188..4194 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4188 
                     /label="KIF11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTCGCAG CCAAATTCGT CTGCGAAGAA GAAAGAGGAG AAGGGGAAGA 
     1081 ACATCCAGGT GGTGGTGAGA TGCAGACCAT TTAATTTGGC AGAGCGGAAA GCTAGCGCCC 
     1141 ATTCAATAGT AGAATGTGAT CCTGTACGAA AAGAAGTTAG TGTACGAACT GGAGGATTGG 
     1201 CTGACAAGAG CTCAAGGAAA ACATACACTT TTGATATGGT GTTTGGAGCA TCTACTAAAC 
     1261 AGATTGATGT TTACCGAAGT GTTGTTTGTC CAATTCTGGA TGAAGTTATT ATGGGCTATA 
     1321 ATTGCACTAT CTTTGCGTAT GGCCAAACTG GCACTGGAAA AACTTTTACA ATGGAAGGTG 
     1381 AAAGGTCACC TAATGAAGAG TATACCTGGG AAGAGGATCC CTTGGCTGGT ATAATTCCAC 
     1441 GTACCCTTCA TCAAATTTTT GAGAAACTTA CTGATAATGG TACTGAATTT TCAGTCAAAG 
     1501 TGTCTCTGTT GGAGATCTAT AATGAAGAGC TTTTTGATCT TCTTAATCCA TCATCTGATG 
     1561 TTTCTGAGAG ACTACAGATG TTTGATGATC CCCGTAACAA GAGAGGAGTG ATAATTAAAG 
     1621 GTTTAGAAGA AATTACAGTA CACAACAAGG ATGAAGTCTA TCAAATTTTA GAAAAGGGGG 
     1681 CAGCAAAAAG GACAACTGCA GCTACTCTGA TGAATGCATA CTCTAGTCGT TCCCACTCAG 
     1741 TTTTCTCTGT TACAATACAT ATGAAAGAAA CTACGATTGA TGGAGAAGAG CTTGTTAAAA 
     1801 TCGGAAAGTT GAACTTGGTT GATCTTGCAG GAAGTGAAAA CATTGGCCGT TCTGGAGCTG 
     1861 TTGATAAGAG AGCTCGGGAA GCTGGAAATA TAAATCAATC CCTGTTGACT TTGGGAAGGG 
     1921 TCATTACTGC CCTTGTAGAA AGAACACCTC ATGTTCCTTA TCGAGAATCT AAACTAACTA 
     1981 GAATCCTCCA GGATTCTCTT GGAGGGCGTA CAAGAACATC TATAATTGCA ACAATTTCTC 
     2041 CTGCATCTCT CAATCTTGAG GAAACTCTGA GTACATTGGA ATATGCTCAT AGAGCAAAGA 
     2101 ACATATTGAA TAAGCCTGAA GTGAATCAGA AACTCACCAA AAAAGCTCTT ATTAAGGAGT 
     2161 ATACGGAGGA GATAGAACGT TTAAAACGAG ATCTTGCTGC AGCCCGTGAG AAAAATGGAG 
     2221 TGTATATTTC TGAAGAAAAT TTTAGAGTCA TGAGTGGAAA ATTAACTGTT CAAGAAGAGC 
     2281 AGATTGTAGA ATTGATTGAA AAAATTGGTG CTGTTGAGGA GGAGCTGAAT AGGGTTACAG 
     2341 AGTTGTTTAT GGATAATAAA AATGAACTTG ACCAGTGTAA ATCTGACCTG CAAAATAAAA 
     2401 CACAAGAACT TGAAACCACT CAAAAACATT TGCAAGAAAC TAAATTACAA CTTGTTAAAG 
     2461 AAGAATATAT CACATCAGCT TTGGAAAGTA CTGAGGAGAA ACTTCATGAT GCTGCCAGCA 
     2521 AGCTGCTTAA CACAGTTGAA GAAACTACAA AAGATGTATC TGGTCTCCAT TCCAAACTGG 
     2581 ATCGTAAGAA GGCAGTTGAC CAACACAATG CAGAAGCTCA GGATATTTTT GGCAAAAACC 
     2641 TGAATAGTCT GTTTAATAAT ATGGAAGAAT TAATTAAGGA TGGCAGCTCA AAGCAAAAGG 
     2701 CCATGCTAGA AGTACATAAG ACCTTATTTG GTAATCTGCT GTCTTCCAGT GTCTCTGCAT 
     2761 TAGATACCAT TACTACAGTA GCACTTGGAT CTCTCACATC TATTCCAGAA AATGTGTCTA 
     2821 CTCATGTTTC TCAGATTTTT AATATGATAC TAAAAGAACA ATCATTAGCA GCAGAAAGTA 
     2881 AAACTGTACT ACAGGAATTG ATTAATGTAC TCAAGACTGA TCTTCTAAGT TCACTGGAAA 
     2941 TGATTTTATC CCCAACTGTG GTGTCTATAC TGAAAATCAA TAGTCAACTA AAGCATATTT 
     3001 TCAAGACTTC ATTGACAGTG GCCGATAAGA TAGAAGATCA AAAAAAGGAA CTAGATGGCT 
     3061 TTCTCAGTAT ACTGTGTAAC AATCTACATG AACTACAAGA AAATACCATT TGTTCCTTGG 
     3121 TTGAGTCACA AAAGCAATGT GGAAACCTAA CTGAAGACCT GAAGACAATA AAGCAGACCC 
     3181 ATTCCCAGGA ACTTTGCAAG TTAATGAATC TTTGGACAGA GAGATTCTGT GCTTTGGAGG 
     3241 AAAAGTGTGA AAATATACAG AAACCACTTA GTAGTGTCCA GGAAAATATA CAGCAGAAAT 
     3301 CTAAGGATAT AGTCAACAAA ATGACTTTTC ACAGTCAAAA ATTTTGTGCT GATTCTGATG 
     3361 GCTTCTCACA GGAACTCAGA AATTTTAACC AAGAAGGTAC AAAATTGGTT GAAGAATCTG 
     3421 TGAAACACTC TGATAAACTC AATGGCAACC TGGAAAAAAT ATCTCAAGAG ACTGAACAGA 
     3481 GATGTGAATC TCTGAACACA AGAACAGTTT ATTTTTCTGA ACAGTGGGTA TCTTCCTTAA 
     3541 ATGAAAGGGA ACAGGAACTT CACAACTTAT TGGAGGTTGT AAGCCAATGT TGTGAGGCTT 
     3601 CAAGTTCAGA CATCACTGAG AAATCAGATG GACGTAAGGC AGCTCATGAG AAACAGCATA 
     3661 ACATTTTTCT TGATCAGATG ACTATTGATG AAGATAAATT GATAGCACAA AATCTAGAAC 
     3721 TTAATGAAAC CATAAAAATT GGTTTGACTA AGCTTAATTG CTTTCTGGAA CAGGATCTGA 
     3781 AACTGGATAT CCCAACAGGT ACGACACCAC AGAGGAAAAG TTATTTATAC CCATCAACAC 
     3841 TGGTAAGAAC TGAACCACGT GAACATCTCC TTGATCAGCT GAAAAGGAAA CAGCCTGAGC 
     3901 TGTTAATGAT GCTAAACTGT TCAGAAAACA ACAAAGAAGA GACAATTCCG GATGTGGATG 
     3961 TAGAAGAGGC AGTTCTGGGG CAGTATACTG AAGAACCTCT AAGTCAAGAG CCATCTGTAG 
     4021 ATGCTGGTGT GGATTGTTCA TCAATTGGCG GGGTTCCATT TTTCCAGCAT AAAAAATCAC 
     4081 ATGGAAAAGA CAAAGAAAAC AGAGGCATTA ACACACTGGA GAGGTCTAAA GTGGAAGAAA 
     4141 CTACAGAGCA CTTGGTTACA AAGAGCAGAT TACCTCTGCG AGCCCAGATC AACCTTACGC 
     4201 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4261 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4321 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4381 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4441 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4501 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4561 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4621 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4681 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4741 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4801 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4861 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4921 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4981 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5041 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5101 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5161 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5221 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5281 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5341 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5401 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5461 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5521 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5581 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5641 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5701 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5761 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5821 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5881 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5941 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6001 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6061 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6121 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6181 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6241 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6301 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6361 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6421 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6481 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6541 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6601 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6661 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6721 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6781 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6841 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6901 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6961 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7021 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7081 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7141 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7201 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7261 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7321 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7381 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7441 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7501 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7561 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7621 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7681 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7741 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7801 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7861 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7921 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7981 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8041 GCGAATTTT
//