LOCUS RC218833 9360 bp DNA circular SYN DEFINITION PLEKHH2 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family H (with MyTH4 domain) member 2 (PLEKHH2) ACCESSION NM_172069 VERSION NM_172069.1 SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 5517..5608 /label="Myc-DDK" misc_feature 5638..6223 /label="PolyA signal" misc_feature 5695..5714 /label="Restriction Enzyme" misc_feature 6499..7171 /label="ColE1" misc_feature 7601..8395 /label="Kan/Neo" misc_feature 8430..8732 /label="SV40 ori" misc_feature 8818..9295 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 5499..5505 /label="Restriction Enzyme" misc_feature 1020..5499 /label="PLEKHH2" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCAGAGCTT TCTGAGCCAG AGGGACCAGT AGATTGGAAG GAACGATGTG 1081 TAGCTCTGGA GTCCCAACTC ATGAAATTTA GAGTTCAAGC AAGCAAGATA CGAGAGCTTT 1141 TAGCAGAGAA GATGCAACAG CTTGAGAGAC AAGTTATTGA TGCTGAACGT CAAGCAGAAA 1201 AAGCTTTTCA ACAGGTACAA GTTATGGAAG ATAAATTAAA AGCAGCTAAT ATTCAAACCA 1261 GTGAATCAGA GACAAGATTA TATAATAAGT GTCAAGATCT GGAGTCGCTA ATACAGGAAA 1321 AAGATGACGT CATTCAAAAC TTGGAATTGC AACTTGAAGA GCAGAAACAA ATAAGAATAC 1381 AAGAAGCTAA AATAATAGAA GAGAAAGCAG CTAAGATAAA AGAATGGGTA ACAGTTAAGT 1441 TAAATGAGCT GGAATTGGAG AATCAGAATC TTCGTTTGAT CAACCAAAAC CAAACTGAAG 1501 AGATAAGAAC AATGCAGTCA AAACTACAAG AAGTTCAAGG AAAGAAGTCA TCCACTGTCT 1561 CTACACTAAA GCTTTCGGAA GGCCAGCGCC TGAGCAGTTT GACCTTTGGG TGCTTTTTAT 1621 CTCGAGCAAG GAGTCCTCCT CAAGTAGTAA AATCTGAGGA AATGAGCAAG ATATCATCGA 1681 AAGAACCTGA GTTCACTGAA GGAAAAGACA TGGAAGAAAT GGAAATTCCA GAAAAGTCTG 1741 TTGATAACCA AGTTCTAGAA AACAACAGAG GCCAGAGAAC ATTGCATCAA ACCCCTTGTG 1801 GCTCAGAACA GAATCGGAAA ACAAGAACAA GCTTTGCCAC AGATGGTGGC ATCTCCCAGA 1861 ATTCTGGGGC TCCTGTGAGT GACTGGAGCT CTGATGAGGA AGACGGGAGC AAAGGAAGAT 1921 CCAAGTCCAG ATGCACATCC ACCCTCTCCA GTCACACATC TGAGGAAGGG GTCCAGTGTA 1981 GCAGGATGGG AAGTGAAATG TATCTGACAG CATCTGATGA CAGCAGCTCT ATATTTGAGG 2041 AAGAGACTTT TGGCATAAAG AGACCAGAAC ACAAGAAGCT ATATTCTTGG CAGCAGGAGG 2101 CACAGTGGAA AGCTCTAAAT AGTCCTCTTG GAAAGGGAAA TTCTGAATTA AGTAAAAAGG 2161 AACAAGATAG TTCCTCGGAT GAACTGAATA AAAAATTTCA ATCCCAGAGA CTCGATTATT 2221 CATCTTCATC GAGTGAAGCC AACACCCCAA GCCCTATTTT GACCCCAGCT TTAATGCCAA 2281 AGCATCCTAA CTCACTCTCT GGAAAAGGAA CACAATTAGT GCCTTCATCA CACCTGCCAC 2341 CCCCAAAGTT AAGGATTCCT AATGTTTTCA GTATAAGTGC AGCACTAGCC AAAAGGCACT 2401 TAAGCCAGCC ACAGTTAAGC TCTGACAGGA TGTTTGGTAC AAATAGAAAC GCTATAAGCA 2461 TGATACGACC ACTGAGACCT CAGGAAACTG ATCTTGATCT AGTTGATGGA GACAGTACAG 2521 AAGTTTTAGA GAATATGGAC ACGAGTTGTG ATGATGGATT ATTTTCCTAT GACTCCTTGG 2581 ACTCTCCAAA TTCAGATGAC CAGGAACACT GTGACTCAGC AAAGAAGGTG GCATACAGCA 2641 AACCTCCAAC TCCTCCCCTG CACCGTTTTC CTTCTTGGGA AAGCAGAATT TATGCTGTAG 2701 CCAAATCAGG TATTCGAATG TCTGAGGCCT TCAATATGGA GAGTGTTAAT AAAAATTCTG 2761 CTGCAACCCT TTCCTATACT ACATCAGGAC TTTATACATC TCTGATATAC AAGAACATGA 2821 CCACCCCAGT GTATACAACT TTGAAGGGGA AGGCGACCCA AATAAGTAGC AGCCCTTTCC 2881 TGGATGACTC ATCTGGGTCA GAGGAAGAAG ACAGCTCCAG ATCCAGCTCC CGGACGTCAG 2941 AGTCAGACTC ACGCAGTAGG AGTGGGCCAG GCAGCCCCAG AGCCATGAAA CGAGGTGTGT 3001 CTCTCTCCTC TGTGGCTTCT GAAAGTGATT ATGCTATTCC TCCTGATGCT TACTCCACAG 3061 ACACGGAGTA CTCACAGCCA GAGCAGAAGC TCCCAAAAAC TTGCTCATCT TCCAGTGATA 3121 ATGGGAAAAA TGAACCACTG GAAAAATCTG GTTATTTATT AAAAATGAGT GGTAAAGTCA 3181 AGTCTTGGAA GCGGCGGTGG TTTGTTCTTA AAGGTTGTGA ATTACTTTAC TACAAATCTC 3241 CGAGTGATGT AATTAGAAAA CCCCAGGGCC ATATTGAACT TAGTGCATCC TGTAGTATTT 3301 TAAGAGGAGA TAACAAACAA ACAGTTCAGT TGACCACTGA AAAACACACA TACTATCTGA 3361 CTGCAGATTC TCCCAATATA TTGGAAGAGT GGATTAAAGT GTTACAGAAT GTTCTTCGAG 3421 TACAAGCTGC CAACCCACTT TCCCTGCAGC CTGAGGGCAA ACCCACCATG AAGGGATTGC 3481 TCACTAAGGT AAAACATGGA TATTCCAAGA GAGTCTGGTG TACACTAATA GGAAAGACAT 3541 TATATTATTT TCGGAGTCAA GAAGATAAGT TTCCTTTAGG TCAGATCAAA CTCTGGGAGG 3601 TTAAAGTGGA AGAGGTTGAC AGATCTTGTG ATTCAGATGA AGATTATGAA GCCAGTGGAC 3661 GAAGTCTGTT ATCCACACAT TATACTATCG TTATCCATCC CAAAGACCAA GGTCCAACTT 3721 ACCTCCTAAT TGGATCCAAG CATGAAAAGG ACACTTGGCT TTATCATCTG ACTGTTGCAG 3781 CTGGAAGCAA CAATGTAAAC GTTGGATCTG AATTTGAACA ACTGGTTTGC AAATTGCTAA 3841 ATATAGACGG GGAGCCTTCC TCTCAGATAT GGAGACACCC CACTTTGTGT CACAGTAAAG 3901 AAGGAATCAT TTCCCCTCTG ACAACTCTAC CTTCCGAAGC CCTGCAGACA GAAGCTATTA 3961 AATTATTTAA GACCTGCCAG CTTTTTATAA ATGCTGCAGT TGACTCTCCT GCAATTGATT 4021 ACCACATATC TTTAGCCCAG AGTGCTTTGC AAATCTGCCT GACACATCCT GAGCTGCAGA 4081 ATGAAATTTG CTGTCAGCTT ATTAAACAGA CAAGACGAAG ACAGCCACAG AATCAACCAG 4141 GACCATTGCA GGGCTGGCAG CTCTTGGCAC TCTGCGTTGG GCTCTTCCTT CCCCATCATC 4201 CTTTCCTGTG GCTCCTCAGG CTTCACCTAA AGAGGAATGC AGATTCCAGG ACAGAATTTG 4261 GAAAATATGC CATTTACTGC CAGCGTTGTG TAGAAAGAAC GCAACAAAAT GGTGACAGAG 4321 AAGCAAGACC CTCAAGGATG GAAATTCTTT CAACTCTTCT CCGAAACCCT TATCACCATT 4381 CTTTGCCCTT TAGTATACCT GTGCACTTCA TGAATGGGAT ATACCAGGTA GTTGGTTTTG 4441 ACGCATCTAC CACAGTGGAA GAATTTTTGA ATACTTTGAA CCAGGACACA GGAATGAGGA 4501 AACCAGCGCA GTCTGGATTT GCGTTGTTCA CTGACGATCC TTCTGGCAGA GATTTAGAGC 4561 ATTGTCTTCA AGGAAACATC AAGATTTGTG ACATTATTTC CAAATGGGAA CAGGCTTCCA 4621 AAGAACAGCA GCCTGGAAAA TGTGAAGGTA CAAGGACTGT TCGTCTGACA TACAAAAACA 4681 GACTATATTT CTCAGTGCAA GCTCGTGGAG AGACTGATAG AGAAAAGTTG CTGTTAATGT 4741 ATCAGACAAA TGATCAAATC ATAAATGGAC TTTTTCCTCT GAACAAAGAT CTGGCATTAG 4801 AAATGGCAGC TCTTTTATCT CAGGTAGAGA TTGGAGATTT TGAAAGACCT TTCTCAACTC 4861 CAGCAGGGCA TGTTACCAAT CAGTGCAAAG TGAATCAAAC TCTAAAGCAA GTCATAGAGA 4921 AATTTTATCC TAAAAGGTAT AGAGATGGCT GTTCTGAAGA GCAGTTAAGG CAGCTTTGCC 4981 AGCGACTTTC AACCAGATGG ATGGCCCTCC GGGGACACAG TGCTGCTGAC TGTGTGCGCA 5041 TTTATTTGAC AGTAGCCAGG AAGTGGCCAT TCTTTGGTGC CAAGTTGTTT CTTGCAAAAC 5101 CCATAACTCC ATCATCACTT GGAAGTACTT TCTTGTGGCT GGCTGTACAT GAGGATGGTT 5161 TAAGCCTCTT AGAATACAAC TCCATGAGGT TAATAGTCAG CTATGTGTAC AAGAGTCTAA 5221 TGACCTTTGG AGGCTATCAA GATGATTTTA TGGTAGTCAT TAACAATACA CATTCAAAGG 5281 ACAAACCAAC AGAGAAATTA CTTTTTGCCA TGGCAAAACC CAAGATTCTT GAAATCACTC 5341 TTTTGATCGC CAGTTACATA AACAACTTCC ATCAGCAAAA GGCAGCATTT CACCACCTCT 5401 CTGCTCCAGC ACTGCTCTCA GCCCAGACCC GGGGACCCCA AGCCAGAATG ATGGGAAGCC 5461 AGCCTCTTCT GTCAAGCAGC AGACCGACCA AAGGCCCCAC CTTACTCACG CGTACGCGGC 5521 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 5581 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 5641 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 5701 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 5761 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 5821 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 5881 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 5941 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 6001 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 6061 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 6121 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 6181 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 6241 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 6301 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 6361 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 6421 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 6481 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 6541 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 6601 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 6661 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 6721 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 6781 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 6841 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 6901 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 6961 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 7021 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 7081 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 7141 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 7201 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 7261 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 7321 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 7381 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 7441 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 7501 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 7561 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 7621 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 7681 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 7741 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 7801 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 7861 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 7921 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 7981 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 8041 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 8101 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 8161 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 8221 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 8281 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 8341 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 8401 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 8461 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 8521 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 8581 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 8641 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 8701 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 8761 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 8821 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 8881 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 8941 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 9001 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 9061 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 9121 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 9181 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 9241 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 9301 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT //