LOCUS       RC218833                9360 bp    DNA     circular  SYN 
DEFINITION  PLEKHH2 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family H (with MyTH4 domain) member 2 (PLEKHH2)
ACCESSION   NM_172069
VERSION     NM_172069.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5517..5608 
                     /label="Myc-DDK"  
     misc_feature    5638..6223 
                     /label="PolyA signal"  
     misc_feature    5695..5714 
                     /label="Restriction Enzyme"  
     misc_feature    6499..7171 
                     /label="ColE1"  
     misc_feature    7601..8395 
                     /label="Kan/Neo"  
     misc_feature    8430..8732 
                     /label="SV40 ori"  
     misc_feature    8818..9295 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5499..5505 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5499 
                     /label="PLEKHH2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGAGCTT TCTGAGCCAG AGGGACCAGT AGATTGGAAG GAACGATGTG 
     1081 TAGCTCTGGA GTCCCAACTC ATGAAATTTA GAGTTCAAGC AAGCAAGATA CGAGAGCTTT 
     1141 TAGCAGAGAA GATGCAACAG CTTGAGAGAC AAGTTATTGA TGCTGAACGT CAAGCAGAAA 
     1201 AAGCTTTTCA ACAGGTACAA GTTATGGAAG ATAAATTAAA AGCAGCTAAT ATTCAAACCA 
     1261 GTGAATCAGA GACAAGATTA TATAATAAGT GTCAAGATCT GGAGTCGCTA ATACAGGAAA 
     1321 AAGATGACGT CATTCAAAAC TTGGAATTGC AACTTGAAGA GCAGAAACAA ATAAGAATAC 
     1381 AAGAAGCTAA AATAATAGAA GAGAAAGCAG CTAAGATAAA AGAATGGGTA ACAGTTAAGT 
     1441 TAAATGAGCT GGAATTGGAG AATCAGAATC TTCGTTTGAT CAACCAAAAC CAAACTGAAG 
     1501 AGATAAGAAC AATGCAGTCA AAACTACAAG AAGTTCAAGG AAAGAAGTCA TCCACTGTCT 
     1561 CTACACTAAA GCTTTCGGAA GGCCAGCGCC TGAGCAGTTT GACCTTTGGG TGCTTTTTAT 
     1621 CTCGAGCAAG GAGTCCTCCT CAAGTAGTAA AATCTGAGGA AATGAGCAAG ATATCATCGA 
     1681 AAGAACCTGA GTTCACTGAA GGAAAAGACA TGGAAGAAAT GGAAATTCCA GAAAAGTCTG 
     1741 TTGATAACCA AGTTCTAGAA AACAACAGAG GCCAGAGAAC ATTGCATCAA ACCCCTTGTG 
     1801 GCTCAGAACA GAATCGGAAA ACAAGAACAA GCTTTGCCAC AGATGGTGGC ATCTCCCAGA 
     1861 ATTCTGGGGC TCCTGTGAGT GACTGGAGCT CTGATGAGGA AGACGGGAGC AAAGGAAGAT 
     1921 CCAAGTCCAG ATGCACATCC ACCCTCTCCA GTCACACATC TGAGGAAGGG GTCCAGTGTA 
     1981 GCAGGATGGG AAGTGAAATG TATCTGACAG CATCTGATGA CAGCAGCTCT ATATTTGAGG 
     2041 AAGAGACTTT TGGCATAAAG AGACCAGAAC ACAAGAAGCT ATATTCTTGG CAGCAGGAGG 
     2101 CACAGTGGAA AGCTCTAAAT AGTCCTCTTG GAAAGGGAAA TTCTGAATTA AGTAAAAAGG 
     2161 AACAAGATAG TTCCTCGGAT GAACTGAATA AAAAATTTCA ATCCCAGAGA CTCGATTATT 
     2221 CATCTTCATC GAGTGAAGCC AACACCCCAA GCCCTATTTT GACCCCAGCT TTAATGCCAA 
     2281 AGCATCCTAA CTCACTCTCT GGAAAAGGAA CACAATTAGT GCCTTCATCA CACCTGCCAC 
     2341 CCCCAAAGTT AAGGATTCCT AATGTTTTCA GTATAAGTGC AGCACTAGCC AAAAGGCACT 
     2401 TAAGCCAGCC ACAGTTAAGC TCTGACAGGA TGTTTGGTAC AAATAGAAAC GCTATAAGCA 
     2461 TGATACGACC ACTGAGACCT CAGGAAACTG ATCTTGATCT AGTTGATGGA GACAGTACAG 
     2521 AAGTTTTAGA GAATATGGAC ACGAGTTGTG ATGATGGATT ATTTTCCTAT GACTCCTTGG 
     2581 ACTCTCCAAA TTCAGATGAC CAGGAACACT GTGACTCAGC AAAGAAGGTG GCATACAGCA 
     2641 AACCTCCAAC TCCTCCCCTG CACCGTTTTC CTTCTTGGGA AAGCAGAATT TATGCTGTAG 
     2701 CCAAATCAGG TATTCGAATG TCTGAGGCCT TCAATATGGA GAGTGTTAAT AAAAATTCTG 
     2761 CTGCAACCCT TTCCTATACT ACATCAGGAC TTTATACATC TCTGATATAC AAGAACATGA 
     2821 CCACCCCAGT GTATACAACT TTGAAGGGGA AGGCGACCCA AATAAGTAGC AGCCCTTTCC 
     2881 TGGATGACTC ATCTGGGTCA GAGGAAGAAG ACAGCTCCAG ATCCAGCTCC CGGACGTCAG 
     2941 AGTCAGACTC ACGCAGTAGG AGTGGGCCAG GCAGCCCCAG AGCCATGAAA CGAGGTGTGT 
     3001 CTCTCTCCTC TGTGGCTTCT GAAAGTGATT ATGCTATTCC TCCTGATGCT TACTCCACAG 
     3061 ACACGGAGTA CTCACAGCCA GAGCAGAAGC TCCCAAAAAC TTGCTCATCT TCCAGTGATA 
     3121 ATGGGAAAAA TGAACCACTG GAAAAATCTG GTTATTTATT AAAAATGAGT GGTAAAGTCA 
     3181 AGTCTTGGAA GCGGCGGTGG TTTGTTCTTA AAGGTTGTGA ATTACTTTAC TACAAATCTC 
     3241 CGAGTGATGT AATTAGAAAA CCCCAGGGCC ATATTGAACT TAGTGCATCC TGTAGTATTT 
     3301 TAAGAGGAGA TAACAAACAA ACAGTTCAGT TGACCACTGA AAAACACACA TACTATCTGA 
     3361 CTGCAGATTC TCCCAATATA TTGGAAGAGT GGATTAAAGT GTTACAGAAT GTTCTTCGAG 
     3421 TACAAGCTGC CAACCCACTT TCCCTGCAGC CTGAGGGCAA ACCCACCATG AAGGGATTGC 
     3481 TCACTAAGGT AAAACATGGA TATTCCAAGA GAGTCTGGTG TACACTAATA GGAAAGACAT 
     3541 TATATTATTT TCGGAGTCAA GAAGATAAGT TTCCTTTAGG TCAGATCAAA CTCTGGGAGG 
     3601 TTAAAGTGGA AGAGGTTGAC AGATCTTGTG ATTCAGATGA AGATTATGAA GCCAGTGGAC 
     3661 GAAGTCTGTT ATCCACACAT TATACTATCG TTATCCATCC CAAAGACCAA GGTCCAACTT 
     3721 ACCTCCTAAT TGGATCCAAG CATGAAAAGG ACACTTGGCT TTATCATCTG ACTGTTGCAG 
     3781 CTGGAAGCAA CAATGTAAAC GTTGGATCTG AATTTGAACA ACTGGTTTGC AAATTGCTAA 
     3841 ATATAGACGG GGAGCCTTCC TCTCAGATAT GGAGACACCC CACTTTGTGT CACAGTAAAG 
     3901 AAGGAATCAT TTCCCCTCTG ACAACTCTAC CTTCCGAAGC CCTGCAGACA GAAGCTATTA 
     3961 AATTATTTAA GACCTGCCAG CTTTTTATAA ATGCTGCAGT TGACTCTCCT GCAATTGATT 
     4021 ACCACATATC TTTAGCCCAG AGTGCTTTGC AAATCTGCCT GACACATCCT GAGCTGCAGA 
     4081 ATGAAATTTG CTGTCAGCTT ATTAAACAGA CAAGACGAAG ACAGCCACAG AATCAACCAG 
     4141 GACCATTGCA GGGCTGGCAG CTCTTGGCAC TCTGCGTTGG GCTCTTCCTT CCCCATCATC 
     4201 CTTTCCTGTG GCTCCTCAGG CTTCACCTAA AGAGGAATGC AGATTCCAGG ACAGAATTTG 
     4261 GAAAATATGC CATTTACTGC CAGCGTTGTG TAGAAAGAAC GCAACAAAAT GGTGACAGAG 
     4321 AAGCAAGACC CTCAAGGATG GAAATTCTTT CAACTCTTCT CCGAAACCCT TATCACCATT 
     4381 CTTTGCCCTT TAGTATACCT GTGCACTTCA TGAATGGGAT ATACCAGGTA GTTGGTTTTG 
     4441 ACGCATCTAC CACAGTGGAA GAATTTTTGA ATACTTTGAA CCAGGACACA GGAATGAGGA 
     4501 AACCAGCGCA GTCTGGATTT GCGTTGTTCA CTGACGATCC TTCTGGCAGA GATTTAGAGC 
     4561 ATTGTCTTCA AGGAAACATC AAGATTTGTG ACATTATTTC CAAATGGGAA CAGGCTTCCA 
     4621 AAGAACAGCA GCCTGGAAAA TGTGAAGGTA CAAGGACTGT TCGTCTGACA TACAAAAACA 
     4681 GACTATATTT CTCAGTGCAA GCTCGTGGAG AGACTGATAG AGAAAAGTTG CTGTTAATGT 
     4741 ATCAGACAAA TGATCAAATC ATAAATGGAC TTTTTCCTCT GAACAAAGAT CTGGCATTAG 
     4801 AAATGGCAGC TCTTTTATCT CAGGTAGAGA TTGGAGATTT TGAAAGACCT TTCTCAACTC 
     4861 CAGCAGGGCA TGTTACCAAT CAGTGCAAAG TGAATCAAAC TCTAAAGCAA GTCATAGAGA 
     4921 AATTTTATCC TAAAAGGTAT AGAGATGGCT GTTCTGAAGA GCAGTTAAGG CAGCTTTGCC 
     4981 AGCGACTTTC AACCAGATGG ATGGCCCTCC GGGGACACAG TGCTGCTGAC TGTGTGCGCA 
     5041 TTTATTTGAC AGTAGCCAGG AAGTGGCCAT TCTTTGGTGC CAAGTTGTTT CTTGCAAAAC 
     5101 CCATAACTCC ATCATCACTT GGAAGTACTT TCTTGTGGCT GGCTGTACAT GAGGATGGTT 
     5161 TAAGCCTCTT AGAATACAAC TCCATGAGGT TAATAGTCAG CTATGTGTAC AAGAGTCTAA 
     5221 TGACCTTTGG AGGCTATCAA GATGATTTTA TGGTAGTCAT TAACAATACA CATTCAAAGG 
     5281 ACAAACCAAC AGAGAAATTA CTTTTTGCCA TGGCAAAACC CAAGATTCTT GAAATCACTC 
     5341 TTTTGATCGC CAGTTACATA AACAACTTCC ATCAGCAAAA GGCAGCATTT CACCACCTCT 
     5401 CTGCTCCAGC ACTGCTCTCA GCCCAGACCC GGGGACCCCA AGCCAGAATG ATGGGAAGCC 
     5461 AGCCTCTTCT GTCAAGCAGC AGACCGACCA AAGGCCCCAC CTTACTCACG CGTACGCGGC 
     5521 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5581 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5641 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5701 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5761 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5821 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5881 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5941 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     6001 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     6061 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     6121 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     6181 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     6241 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     6301 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     6361 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6421 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6481 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6541 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6601 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6661 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6721 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6781 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6841 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6901 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6961 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     7021 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     7081 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     7141 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     7201 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     7261 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     7321 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     7381 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7441 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7501 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7561 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7621 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7681 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7741 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7801 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7861 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7921 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7981 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     8041 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     8101 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     8161 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     8221 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     8281 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     8341 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     8401 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8461 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8521 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8581 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8641 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8701 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8761 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8821 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8881 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8941 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     9001 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     9061 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     9121 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     9181 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     9241 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     9301 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//