LOCUS       RC218796L1                9246 bp    DNA     circular  SYN 
DEFINITION  KIF5C (Myc-DDK-tagged)-Human kinesin family member 5C (KIF5C)
ACCESSION   NM_004522
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7537..7717 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8445..9104 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7876..8444 
                     /label="ColE1"  
     misc_feature    6412..6432 
                     /label="LR50 primer"  
     misc_feature    6209..6280 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6421..7012 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6191..6197 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6191 
                     /label="KIF5C"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGATCCA GCCGAATGCA GCATCAAAGT 
     3361 GATGTGCCGG TTCCGGCCCC TCAACGAAGC GGAGATCCTC CGCGGGGACA AATTCATCCC 
     3421 CAAATTTAAA GGCGATGAGA CCGTGGTGAT CGGGCAAGGG AAGCCATATG TCTTCGACAG 
     3481 AGTGCTACCT CCCAACACGA CCCAAGAGCA GGTTTACAAT GCATGTGCGA AGCAAATTGT 
     3541 CAAAGATGTC CTTGAAGGTT ATAACGGGAC GATTTTTGCG TATGGGCAGA CTTCATCAGG 
     3601 AAAAACCCAC ACCATGGAGG GGAAGCTGCA TGACCCCCAG CTCATGGGGA TCATCCCACG 
     3661 AATTGCCCAT GATATCTTTG ACCATATCTA CTCCATGGAT GAGAACCTGG AGTTTCACAT 
     3721 AAAGGTTTCC TATTTTGAGA TCTACTTGGA CAAAATAAGG GACTTACTTG ATGTATCCAA 
     3781 GACCAACTTG GCTGTTCATG AAGATAAAAA CAGAGTCCCG TATGTAAAGG GGTGCACTGA 
     3841 GCGGTTTGTG TCGAGCCCTG AGGAAGTCAT GGATGTAATA GATGAAGGCA AAGCAAACCG 
     3901 ACACGTGGCT GTGACAAACA TGAATGAACA CAGCTCTAGA AGTCACAGTA TCTTCCTGAT 
     3961 AAATATTAAA CAAGAGAATG TAGAGACTGA AAAAAAACTC AGTGGGAAAC TTTATTTGGT 
     4021 TGATTTGGCT GGGAGCGAAA AGGTCAGCAA AACTGGTGCC GAGGGAGCTG TTCTTGACGA 
     4081 AGCTAAAAAT ATCAATAAGT CTTTGTCTGC TCTTGGAAAT GTGATCTCTG CTTTGGCAGA 
     4141 AGGGACAAAA ACACATGTGC CATACCGGGA CAGCAAGATG ACTCGGATTC TTCAGGACTC 
     4201 TTTGGGTGGG AACTGCAGAA CCACCATCGT CATTTGCTGT TCTCCTTCTG TCTTCAATGA 
     4261 GGCTGAGACC AAGTCCACAC TGATGTTCGG ACAGAGAGCT AAGACCATCA AGAATACAGT 
     4321 CTCTGTGAAC CTAGAACTGA CAGCAGAAGA ATGGAAGAAG AAATATGAAA AAGAGAAAGA 
     4381 GAAAAACAAG ACTTTGAAGA ATGTTATCCA GCATCTGGAG ATGGAGCTAA ACAGGTGGAG 
     4441 GAATGGAGAA GCTGTGCCTG AGGATGAACA GATCAGTGCC AAGGACCAGA AGAACCTGGA 
     4501 GCCTTGTGAT AACACCCCCA TCATAGACAA TATTGCTCCT GTTGTTGCTG GCATCTCTAC 
     4561 AGAGGAGAAA GAGAAGTACG ATGAGGAGAT CTCCAGTCTC TACAGACAAC TGGATGACAA 
     4621 GGATGATGAA ATTAACCAGC AGAGCCAGCT GGCTGAAAAG CTGAAGCAAC AGATGTTGGA 
     4681 TCAGGATGAG CTTTTAGCTT CCACAAGAAG AGACTATGAG AAGATACAGG AGGAGCTGAC 
     4741 ACGTCTCCAG ATTGAAAATG AGGCAGCCAA GGATGAGGTG AAAGAAGTTC TCCAGGCCCT 
     4801 GGAGGAGCTG GCTGTCAATT ATGACCAGAA ATCACAGGAA GTGGAGGATA AGACCCGGGC 
     4861 CAATGAGCAG CTGACAGACG AGCTGGCCCA GAAAACGACT ACATTGACAA CCACACAGAG 
     4921 AGAGCTGAGC CAGCTACAAG AGCTTAGCAA CCACCAGAAG AAAAGGGCAA CTGAGATCCT 
     4981 GAATTTGCTG TTGAAAGATC TGGGGGAGAT AGGTGGAATT ATTGGCACCA ATGATGTGAA 
     5041 AACTTTGGCA GATGTGAATG GAGTCATTGA GGAGGAGTTT ACCATGGCCC GCCTGTACAT 
     5101 CAGCAAGATG AAGTCAGAGG TCAAGTCCCT GGTGAACCGC AGCAAACAGC TCGAGAGCGC 
     5161 CCAGATGGAC TCCAACAGGA AGATGAATGC CAGCGAGCGG GAGCTGGCAG CCTGCCAGCT 
     5221 GCTCATCTCC CAGCACGAAG CCAAGATCAA GTCTCTGACA GACTACATGC AGAACATGGA 
     5281 ACAGAAGAGG AGGCAGCTAG AAGAGTCCCA GGACTCGCTC AGCGAAGAGC TGGCAAAGCT 
     5341 CCGAGCCCAG GAAAAAATGC ACGAAGTCAG CTTCCAGGAT AAGGAGAAGG AACATCTGAC 
     5401 GCGGTTGCAG GATGCTGAAG AAATGAAGAA GGCGCTGGAG CAGCAGATGG AGAGCCACCG 
     5461 GGAAGCTCAC CAGAAGCAGC TGTCCAGACT CCGAGACGAA ATTGAGGAGA AGCAGAAAAT 
     5521 CATTGATGAG ATTCGGGATT TGAATCAGAA ACTGCAACTG GAACAGGAGA AGCTTAGTTC 
     5581 TGATTATAAC AAGCTGAAAA TAGAGGACCA AGAGAGAGAA ATGAAGCTGG AAAAGCTCTT 
     5641 ATTGCTCAAC GATAAAAGGG AACAAGCCAG AGAAGACCTC AAAGGGCTGG AGGAGACAGT 
     5701 GTCTAGAGAA TTGCAGACAC TGCACAACCT TCGGAAACTC TTTGTCCAGG ATCTGACCAC 
     5761 CCGAGTTAAA AAAAGTGTGG AGTTGGACAA CGATGATGGA GGGGGCAGTG CTGCCCAGAA 
     5821 GCAGAAAATT TCCTTCTTGG AGAATAACCT GGAGCAGCTC ACCAAAGTTC ACAAGCAGCT 
     5881 GGTCCGGGAC AACGCAGACC TGCGCTGTGA ACTGCCCAAG CTGGAGAAGC GGCTGCGTGC 
     5941 CACGGCGGAG CGCGTCAAGG CTCTGGAGAG CGCGCTGAAG GAGGCCAAGG AGAACGCCAT 
     6001 GCGGGACCGT AAGCGCTACC AGCAGGAGGT GGATCGTATC AAGGAGGCCG TGCGGGCCAA 
     6061 GAACATGGCC AGAAGGGCCC ATTCAGCCCA GATCGCCAAG CCCATCCGCC CCGGACACTA 
     6121 CCCGGCCTCA TCTCCAACGG CCGTCCATGC CATTCGAGGG GGAGGAGGCA GCTCTTCAAA 
     6181 TTCCACTCAC TACCAGAAAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA 
     6241 GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC 
     6301 CGGCCGCGGT CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT ATAGCATACA 
     6361 TTATACGAAG TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT CGATATCAAG 
     6421 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC 
     6481 TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT 
     6541 GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT 
     6601 GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA 
     6661 ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC 
     6721 CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG 
     6781 GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT 
     6841 TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT 
     6901 TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT 
     6961 CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT 
     7021 CGATACCGTC GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC 
     7081 AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT 
     7141 TCCAGTCACA CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG 
     7201 CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA 
     7261 TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC AGAACTACAC 
     7321 ACCAGGGCCA GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT 
     7381 TGAGCAAGAG AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT TACACCCTGT 
     7441 GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT TTGACAGCCG 
     7501 CCTAGCATTT CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT CTCTCTGGTT 
     7561 AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA 
     7621 ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA 
     7681 CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA TGTGAGCAAA 
     7741 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     7801 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     7861 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     7921 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     7981 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     8041 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     8101 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     8161 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     8221 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     8281 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     8341 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     8401 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGATTACGCC 
     8461 CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG 
     8521 CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC 
     8581 GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT 
     8641 AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA 
     8701 AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG 
     8761 TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT 
     8821 TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT 
     8881 TTCATTGCCA TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG 
     8941 GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC 
     9001 TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA 
     9061 CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATACTCTTC 
     9121 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     9181 GAATGTATTT AGAAAAATAA ACAAATAGGG GTCCCGCGCA CATTTCCCCG AAAAGTGCCA 
     9241 CCTGAC
//