LOCUS       RC218796                7752 bp    DNA     circular  SYN 
DEFINITION  KIF5C (Myc-DDK-tagged)-Human kinesin family member 5C (KIF5C)
ACCESSION   NM_004522
VERSION     NM_004522.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3909..4000 
                     /label="Myc-DDK"  
     misc_feature    4030..4615 
                     /label="PolyA signal"  
     misc_feature    4087..4106 
                     /label="Restriction Enzyme"  
     misc_feature    4891..5563 
                     /label="ColE1"  
     misc_feature    5993..6787 
                     /label="Kan/Neo"  
     misc_feature    6822..7124 
                     /label="SV40 ori"  
     misc_feature    7210..7687 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3891..3897 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3891 
                     /label="KIF5C"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGATCCA GCCGAATGCA GCATCAAAGT GATGTGCCGG TTCCGGCCCC 
     1081 TCAACGAAGC GGAGATCCTC CGCGGGGACA AATTCATCCC CAAATTTAAA GGCGATGAGA 
     1141 CCGTGGTGAT CGGGCAAGGG AAGCCATATG TCTTCGACAG AGTGCTACCT CCCAACACGA 
     1201 CCCAAGAGCA GGTTTACAAT GCATGTGCGA AGCAAATTGT CAAAGATGTC CTTGAAGGTT 
     1261 ATAACGGGAC GATTTTTGCG TATGGGCAGA CTTCATCAGG AAAAACCCAC ACCATGGAGG 
     1321 GGAAGCTGCA TGACCCCCAG CTCATGGGGA TCATCCCACG AATTGCCCAT GATATCTTTG 
     1381 ACCATATCTA CTCCATGGAT GAGAACCTGG AGTTTCACAT AAAGGTTTCC TATTTTGAGA 
     1441 TCTACTTGGA CAAAATAAGG GACTTACTTG ATGTATCCAA GACCAACTTG GCTGTTCATG 
     1501 AAGATAAAAA CAGAGTCCCG TATGTAAAGG GGTGCACTGA GCGGTTTGTG TCGAGCCCTG 
     1561 AGGAAGTCAT GGATGTAATA GATGAAGGCA AAGCAAACCG ACACGTGGCT GTGACAAACA 
     1621 TGAATGAACA CAGCTCTAGA AGTCACAGTA TCTTCCTGAT AAATATTAAA CAAGAGAATG 
     1681 TAGAGACTGA AAAAAAACTC AGTGGGAAAC TTTATTTGGT TGATTTGGCT GGGAGCGAAA 
     1741 AGGTCAGCAA AACTGGTGCC GAGGGAGCTG TTCTTGACGA AGCTAAAAAT ATCAATAAGT 
     1801 CTTTGTCTGC TCTTGGAAAT GTGATCTCTG CTTTGGCAGA AGGGACAAAA ACACATGTGC 
     1861 CATACCGGGA CAGCAAGATG ACTCGGATTC TTCAGGACTC TTTGGGTGGG AACTGCAGAA 
     1921 CCACCATCGT CATTTGCTGT TCTCCTTCTG TCTTCAATGA GGCTGAGACC AAGTCCACAC 
     1981 TGATGTTCGG ACAGAGAGCT AAGACCATCA AGAATACAGT CTCTGTGAAC CTAGAACTGA 
     2041 CAGCAGAAGA ATGGAAGAAG AAATATGAAA AAGAGAAAGA GAAAAACAAG ACTTTGAAGA 
     2101 ATGTTATCCA GCATCTGGAG ATGGAGCTAA ACAGGTGGAG GAATGGAGAA GCTGTGCCTG 
     2161 AGGATGAACA GATCAGTGCC AAGGACCAGA AGAACCTGGA GCCTTGTGAT AACACCCCCA 
     2221 TCATAGACAA TATTGCTCCT GTTGTTGCTG GCATCTCTAC AGAGGAGAAA GAGAAGTACG 
     2281 ATGAGGAGAT CTCCAGTCTC TACAGACAAC TGGATGACAA GGATGATGAA ATTAACCAGC 
     2341 AGAGCCAGCT GGCTGAAAAG CTGAAGCAAC AGATGTTGGA TCAGGATGAG CTTTTAGCTT 
     2401 CCACAAGAAG AGACTATGAG AAGATACAGG AGGAGCTGAC ACGTCTCCAG ATTGAAAATG 
     2461 AGGCAGCCAA GGATGAGGTG AAAGAAGTTC TCCAGGCCCT GGAGGAGCTG GCTGTCAATT 
     2521 ATGACCAGAA ATCACAGGAA GTGGAGGATA AGACCCGGGC CAATGAGCAG CTGACAGACG 
     2581 AGCTGGCCCA GAAAACGACT ACATTGACAA CCACACAGAG AGAGCTGAGC CAGCTACAAG 
     2641 AGCTTAGCAA CCACCAGAAG AAAAGGGCAA CTGAGATCCT GAATTTGCTG TTGAAAGATC 
     2701 TGGGGGAGAT AGGTGGAATT ATTGGCACCA ATGATGTGAA AACTTTGGCA GATGTGAATG 
     2761 GAGTCATTGA GGAGGAGTTT ACCATGGCCC GCCTGTACAT CAGCAAGATG AAGTCAGAGG 
     2821 TCAAGTCCCT GGTGAACCGC AGCAAACAGC TCGAGAGCGC CCAGATGGAC TCCAACAGGA 
     2881 AGATGAATGC CAGCGAGCGG GAGCTGGCAG CCTGCCAGCT GCTCATCTCC CAGCACGAAG 
     2941 CCAAGATCAA GTCTCTGACA GACTACATGC AGAACATGGA ACAGAAGAGG AGGCAGCTAG 
     3001 AAGAGTCCCA GGACTCGCTC AGCGAAGAGC TGGCAAAGCT CCGAGCCCAG GAAAAAATGC 
     3061 ACGAAGTCAG CTTCCAGGAT AAGGAGAAGG AACATCTGAC GCGGTTGCAG GATGCTGAAG 
     3121 AAATGAAGAA GGCGCTGGAG CAGCAGATGG AGAGCCACCG GGAAGCTCAC CAGAAGCAGC 
     3181 TGTCCAGACT CCGAGACGAA ATTGAGGAGA AGCAGAAAAT CATTGATGAG ATTCGGGATT 
     3241 TGAATCAGAA ACTGCAACTG GAACAGGAGA AGCTTAGTTC TGATTATAAC AAGCTGAAAA 
     3301 TAGAGGACCA AGAGAGAGAA ATGAAGCTGG AAAAGCTCTT ATTGCTCAAC GATAAAAGGG 
     3361 AACAAGCCAG AGAAGACCTC AAAGGGCTGG AGGAGACAGT GTCTAGAGAA TTGCAGACAC 
     3421 TGCACAACCT TCGGAAACTC TTTGTCCAGG ATCTGACCAC CCGAGTTAAA AAAAGTGTGG 
     3481 AGTTGGACAA CGATGATGGA GGGGGCAGTG CTGCCCAGAA GCAGAAAATT TCCTTCTTGG 
     3541 AGAATAACCT GGAGCAGCTC ACCAAAGTTC ACAAGCAGCT GGTCCGGGAC AACGCAGACC 
     3601 TGCGCTGTGA ACTGCCCAAG CTGGAGAAGC GGCTGCGTGC CACGGCGGAG CGCGTCAAGG 
     3661 CTCTGGAGAG CGCGCTGAAG GAGGCCAAGG AGAACGCCAT GCGGGACCGT AAGCGCTACC 
     3721 AGCAGGAGGT GGATCGTATC AAGGAGGCCG TGCGGGCCAA GAACATGGCC AGAAGGGCCC 
     3781 ATTCAGCCCA GATCGCCAAG CCCATCCGCC CCGGACACTA CCCGGCCTCA TCTCCAACGG 
     3841 CCGTCCATGC CATTCGAGGG GGAGGAGGCA GCTCTTCAAA TTCCACTCAC TACCAGAAAA 
     3901 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3961 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4021 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4081 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4141 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4201 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4261 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4321 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4381 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4441 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4501 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4561 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4621 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4681 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4741 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4801 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4861 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4921 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4981 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5041 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5101 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5161 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5221 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5281 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5341 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5401 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5461 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5521 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5581 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5641 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5701 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5761 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5821 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5881 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5941 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6001 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6061 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6121 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6181 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6241 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6301 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6361 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6421 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6481 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6541 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6601 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6661 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6721 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6781 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6841 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6901 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6961 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7021 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7081 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7141 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7201 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7261 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7321 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7381 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7441 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7501 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7561 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7621 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7681 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7741 AACGCGAATT TT
//