LOCUS       RC218730                9255 bp    DNA     circular  SYN 
DEFINITION  PLB1 (Myc-DDK-tagged)-Human phospholipase B1 (PLB1), transcript variant 1
ACCESSION   NM_153021
VERSION     NM_153021.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5412..5503 
                     /label="Myc-DDK"  
     misc_feature    5533..6118 
                     /label="PolyA signal"  
     misc_feature    5590..5609 
                     /label="Restriction Enzyme"  
     misc_feature    6394..7066 
                     /label="ColE1"  
     misc_feature    7496..8290 
                     /label="Kan/Neo"  
     misc_feature    8325..8627 
                     /label="SV40 ori"  
     misc_feature    8713..9190 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5394..5400 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5394 
                     /label="PLB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCTGCGG CCAGGCATTT TCCTCCTGGA GCTGCTGCTG CTTCTGGGGC 
     1081 AAGGGACCCC TCAGATCCAT ACCTCTCCTA GAAAGAGTAC ATTGGAAGGG CAGCTATGGC 
     1141 CAGAGACCCT GAAGAATTCT CCATTCCCAT GCAACCCAAA TAAATTAGGA GTGAATATGC 
     1201 CTTCTAAATC AGTTCACTCT CTGAAGCCTT CTGATATTAA ATTTGTGGCA GCCATTGGCA 
     1261 ATCTGGAAAT TCCTCCAGAC CCAGGGACGG GCGATCTGGA GAAGCAAGAC TGGACTGAAA 
     1321 GGCCACAGCA GGTGTGCATG GGAGTGATGA CAGTCCTTTC AGACATCATC AGATATTTCA 
     1381 GTCCTTCTGT TCCAATGCCT GTGTGCCACA CTGGAAAGAG AGTCATACCC CACGATGGTG 
     1441 CTGAAGACTT GTGGATTCAG GCTCAAGAAC TGGTGAGAAA CATGAAAGAG AACCTGCAAC 
     1501 TTGACTTTCA ATTTGACTGG AAGCTCATCA ATGTGTTCTT CAGTAATGCA AGCCAGTGTT 
     1561 ACCTGTGCCC CTCTGCTCAA CAGAATGGGC TTGCGGCGGG CGGCGTGGAT GAGCTGATGG 
     1621 GGGTGCTGGA CTACCTGCAG CAGGAGGTCC CCAGAGCATT TGTAAACCTG GTGGACCTCT 
     1681 CTGAGGTTGC AGAGGTCTCT CGTCAGTATC ACGGCACTTG GCTCAGCCCT GCACCAGAGC 
     1741 CCTGTAATTG CTCAGAGGAG ACCACCCGGC TGGCCAAGGT GGTGATGCAG TGGTCTTATC 
     1801 AGGAAGCCTG GAACAGCCTC CTGGCCTCCA GCAGGTACAG TGAGCAGGAG TCCTTCACCG 
     1861 TGGTTTTCCA GCCTTTCTTC TATGAGACCA CCCCATCTCT ACACTCGGAG GACCCCCGAC 
     1921 TCCAGGATTC TACCACGCTG GCCTGGCATC TCTGGAATAG GATGATGGAG CCAGCAGGAG 
     1981 AGAAAGATGA GCCATTGAGT GTAAAACACG GGAGGCCAAT GAAGTGTCCC TCTCAGGAGA 
     2041 GCCCCTATCT GTTCAGCTAC AGAAACAGCA ACTACCTGAC CAGACTGCAG AAACCCCAAG 
     2101 ACAAGCTTGA GGTAAGAGAA GGAGCGGAAA TCAGATGTCC TGACAAAGAC CCCTCCGATA 
     2161 CGGTTCCCAC CTCAGTTCAT AGGCTGAAGC CGGCTGACAT CAACGTAATT GGAGCCCTGG 
     2221 GTGACTCTCT CACGGCAGGC AATGGGGCCG GGTCCACACC TGGGAACGTC TTGGACGTCT 
     2281 TGACTCAGTA CCGAGGCCTG TCCTGGAGCG TCGGCGGAGA TGAGAACATC GGCACCGTTA 
     2341 CCACCCTGGC GAACATCCTC CGGGAATTCA ACCCTTCCCT GAAGGGCTTC TCTGTTGGCA 
     2401 CTGGGAAAGA AACCAGTCCT AATGCCTTCT TAAACCAGGC TGTGGCAGGA GGCCGAGCTG 
     2461 AGGATCTACC TGTCCAGGCC AGGAGGCTGG TGGACCTGAT GAAGAATGAC ACGAGGATAC 
     2521 ACTTTCAGGA AGACTGGAAG ATAATAACCC TGTTTATAGG CGGCAATGAC CTCTGTGATT 
     2581 TCTGCAATGA TCTGGTCCAC TATTCTCCCC AGAACTTCAC AGACAACATT GGAAAGGCCC 
     2641 TGGACATCCT CCATGCTGAG GTTCCTCGGG CATTTGTGAA CCTGGTGACG GTGCTTGAGA 
     2701 TCGTCAACCT GAGGGAGCTG TACCAGGAGA AAAAAGTCTA CTGCCCAAGG ATGATCCTCA 
     2761 GGTCTCTGTG TCCCTGTGTC CTGAAGTTTG ATGATAACTC AACAGAACTT GCTACCCTCA 
     2821 TCGAATTCAA CAAGAAGTTT CAGGAGAAGA CCCACCAACT GATTGAGAGT GGGCGATATG 
     2881 ACACAAGGGA AGATTTTACT GTGGTTGTGC AGCCGTTCTT TGAAAACGTG GACATGCCAA 
     2941 AGACCTCGGA AGGATTGCCT GACAACTCTT TCTTCGCTCC TGACTGTTTC CACTTCAGCA 
     3001 GCAAGTCTCA CTCCCGAGCA GCCAGTGCTC TCTGGAACAA TATGCTGGAG CCTGTTGGCC 
     3061 AGAAGACGAC TCGTCATAAG TTTGAAAACA AGATCAATAT CACATGTCCG AACCAGGTCC 
     3121 AGCCGTTTCT GAGGACCTAC AAGAACAGCA TGCAGGGTCA TGGGACCTGG CTGCCATGCA 
     3181 GGGACAGAGC CCCTTCTGCC TTGCACCCTA CCTCAGTGCA TGCCCTGAGA CCTGCAGACA 
     3241 TCCAAGTTGT GGCTGCTCTG GGGGATTCTC TGACCGCTGG CAATGGAATT GGCTCCAAAC 
     3301 CAGACGACCT CCCCGATGTC ACCACACAGT ATCGGGGACT GTCATACAGT GCAGGAGGGG 
     3361 ACGGCTCCCT GGAGAATGTG ACCACCTTAC CTAATATCCT TCGGGAGTTT AACAGAAACC 
     3421 TCACAGGCTA CGCCGTGGGC ACGGGTGATG CCAATGACAC GAATGCATTC CTCAATCAAG 
     3481 CTGTTCCCGG AGCAAAGGCT GAGGATCTTA TGAGCCAAGT CCAAACTCTG ATGCAGAAGA 
     3541 TGAAAGATGA TCATAGAGTA AATTTCCATG AAGACTGGAA GGTCATCACA GTGCTGATCG 
     3601 GAGGCAGCGA TTTATGTGAC TACTGCACAG ATTCGAATCT GTATTCTGCA GCCAACTTTG 
     3661 TTGACCATCT CCGCAATGCC TTGGACGTCC TGCATAGAGA GGTGCCCAGA GTCCTGGTCA 
     3721 ACCTCGTGGA CTTCCTGAAC CCCACTATCA TGCGGCAGGT GTTCCTGGGA AACCCAGACA 
     3781 AGTGCCCAGT GCAGCAGGCC AGCGTTTTGT GTAACTGCGT TCTGACCCTG CGGGAGAACT 
     3841 CCCAAGAGCT AGCCAGGCTG GAGGCCTTCA GCCGAGCCTA CCGGAGCAGC ATGCGCGAGC 
     3901 TGGTGGGGTC AGGCCGCTAT GACACGCAGG AGGACTTCTC TGTGGTGCTG CAGCCCTTCT 
     3961 TCCAGAACAT CCAGCTCCCT GTCCTGGCGG ATGGGCTCCC AGATACGTCC TTCTTTGCCC 
     4021 CAGACTGCAT CCACCCAAAT CAGAAATTCC ACTCCCAGCT GGCCAGAGCC CTTTGGACCA 
     4081 ATATGCTTGA ACCACTTGGA AGCAAAACAG AGACCCTGGA CCTGAGAGCA GAGATGCCCA 
     4141 TCACCTGTCC CACTCAGAAT GAGCCCTTCC TGAGAACCCC TCGGAATAGT AACTACACGT 
     4201 ACCCCATCAA GCCAGCCATT GAGAACTGGG GCAGTGACTT CCTGTGTACA GAGTGGAAGG 
     4261 CTTCCAATAG TGTTCCAACC TCTGTCCACC AGCTCCGACC AGCAGACATC AAAGTGGTGG 
     4321 CCGCCCTGGG TGACTCTCTG ACTACAGCAG TGGGAGCTCG ACCAAACAAC TCCAGTGACC 
     4381 TACCCACATC TTGGAGGGGA CTCTCTTGGA GCATTGGAGG GGATGGGAAC TTGGAGACTC 
     4441 ACACCACACT GCCCAACATT CTGAAGAAGT TCAACCCTTA CCTCCTTGGC TTCTCTACCA 
     4501 GCACCTGGGA GGGGACAGCA GGACTAAATG TGGCAGCGGA AGGGGCCAGA GCTAGGGACA 
     4561 TGCCAGCCCA GGCCTGGGAC CTGGTAGAGC GAATGAAAAA CAGCCCCGAC ATCAACCTGG 
     4621 AGAAAGACTG GAAGCTGGTC ACACTCTTCA TTGGGGTCAA CGACTTGTGT CATTACTGTG 
     4681 AGAATCCGGA GGCCCACTTG GCCACGGAAT ATGTTCAGCA CATCCAACAG GCCCTGGACA 
     4741 TCCTCTCTGA GGAGCTCCCA AGGGCTTTCG TCAACGTGGT GGAGGTCATG GAGCTGGCTA 
     4801 GCCTGTACCA GGGCCAAGGC GGGAAATGTG CCATGCTGGC AGCTCAGAAC AACTGCACTT 
     4861 GCCTCAGACA CTCGCAAAGC TCCCTGGAGA AGCAAGAACT GAAGAAAGTG AACTGGAACC 
     4921 TCCAGCATGG CATCTCCAGT TTCTCCTACT GGCACCAATA CACACAGCGT GAGGACTTTG 
     4981 CGGTTGTGGT GCAGCCTTTC TTCCAAAACA CACTCACCCC ACTGAACGAG AGAGGGGACA 
     5041 CTGACCTCAC CTTCTTCTCC GAGGACTGTT TTCACTTCTC AGACCGCGGG CATGCCGAGA 
     5101 TGGCCATCGC ACTCTGGAAC AACATGCTGG AACCAGTGGG CCGCAAGACT ACCTCCAACA 
     5161 ACTTCACCCA CAGCCGAGCC AAACTCAAGT GCCCCTCTCC TGAGAGCCCT TACCTCTACA 
     5221 CCCTGCGGAA CAGCCGATTG CTCCCAGACC AGGCTGAAGA AGCCCCCGAG GTGCTCTACT 
     5281 GGGCTGTCCC AGTGGCAGCG GGAGTCGGCC TTGTGGTGGG CATCATCGGG ACAGTGGTCT 
     5341 GGAGGTGCAG GAGAGGTGGC CGGAGGGAAG ATCCTCCAAT GAGCCTGCGC ACTGTGGCCC 
     5401 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5461 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5521 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5581 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5641 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5701 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5761 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5821 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5881 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5941 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     6001 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     6061 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     6121 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     6181 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6241 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6301 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6361 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6421 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6481 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6541 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6601 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6661 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6721 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6781 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6841 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6901 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6961 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     7021 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     7081 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     7141 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     7201 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7261 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7321 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7381 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7441 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7501 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7561 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7621 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7681 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7741 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7801 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7861 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7921 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7981 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     8041 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     8101 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     8161 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     8221 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8281 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8341 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8401 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8461 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8521 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8581 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8641 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8701 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8761 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8821 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8881 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8941 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     9001 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     9061 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     9121 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     9181 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9241 TTTAACGCGA ATTTT
//