LOCUS       RC218704                7644 bp    DNA     circular  SYN 
DEFINITION  COL6A2 (Myc-DDK-tagged)-Human collagen, type VI, alpha 2 (COL6A2), transcript variant 2C2a
ACCESSION   NM_058174
VERSION     NM_058174.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3799..3890 
                     /label="Myc-DDK"  
     misc_feature    3920..4505 
                     /label="PolyA signal"  
     misc_feature    3977..3996 
                     /label="Restriction Enzyme"  
     misc_feature    4781..5453 
                     /label="ColE1"  
     misc_feature    5883..6677 
                     /label="Kan/Neo"  
     misc_feature    6712..7014 
                     /label="SV40 ori"  
     misc_feature    7100..7577 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3774..3781 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3774 
                     /label="COL6A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCCAGGGC ACCTGCTCCG TGCTCCTGCT CTGGGGAATC CTGGGGGCCA 
     1081 TCCAGGCCCA GCAGCAGGAG GTCATCTCGC CGGACACTAC CGAGAGAAAC AACAACTGCC 
     1141 CAGAGAAGAC CGACTGCCCC ATCCACGTGT ACTTCGTGCT GGACACCTCG GAGAGCGTCA 
     1201 CCATGCAGTC CCCCACGGAC ATCCTGCTCT TCCACATGAA GCAGTTCGTG CCGCAGTTCA 
     1261 TCAGCCAGCT GCAGAACGAG TTCTACCTGG ACCAGGTGGC GCTGAGCTGG CGCTACGGCG 
     1321 GCCTGCACTT CTCTGACCAG GTGGAGGTGT TCAGCCCACC GGGCAGCGAC CGGGCCTCCT 
     1381 TCATCAAGAA CCTGCAGGGC ATCAGCTCCT TCCGCCGCGG CACCTTCACC GACTGCGCGC 
     1441 TGGCCAACAT GACGGAGCAG ATCCGGCAGG ACCGCAGCAA GGGCACCGTC CACTTCGCCG 
     1501 TGGTCATCAC CGACGGCCAC GTCACCGGCA GCCCCTGCGG GGGCATCAAG CTGCAGGCCG 
     1561 AGCGGGCCCG CGAGGAGGGC ATCCGGCTCT TCGCCGTGGC CCCCAACCAG AACCTGAAGG 
     1621 AGCAGGGCCT GCGGGACATC GCCAGCACGC CGCACGAGCT CTACCGCAAC GACTACGCCA 
     1681 CCATGCTGCC CGACTCCACC GAGATCAACC AGGACACCAT CAACCGCATC ATCAAGGTCA 
     1741 TGAAACACGA AGCCTACGGA GAGTGCTACA AGGTGAGCTG CCTGGAAATC CCTGGGCCCT 
     1801 CTGGGCCCAA GGGCTACCGT GGACAGAAGG GTGCCAAGGG CAACATGGGT GAGCCGGGAG 
     1861 AGCCTGGCCA GAAGGGAAGA CAGGGAGACC CGGGCATCGA AGGCCCCATT GGATTCCCAG 
     1921 GACCCAAGGG CGTTCCTGGC TTCAAAGGAG AGAAGGGTGA ATTTGGAGCC GACGGTCGCA 
     1981 AGGGGGCCCC TGGCCTGGCT GGCAAGAACG GGACCGATGG ACAGAAGGGC AAGCTGGGGC 
     2041 GCATCGGACC TCCTGGCTGC AAGGGAGACC CTGGAAACCG GGGCCCCGAC GGTTACCCGG 
     2101 GGGAAGCAGG GAGTCCAGGG GAGCGAGGAG ACCAAGGCGG CAAGGGGGAC CCTGGCCGCC 
     2161 CAGGACGCAG AGGGCCCCCG GGAGAAATCG GGGCCAAGGG AAGCAAGGGG TATCAAGGCA 
     2221 ACAATGGAGC CCCAGGAAGT CCTGGTGTGA AAGGAGCCAA GGGCGGGCCT GGGCCCCGCG 
     2281 GACCCAAAGG CGAGCCGGGG CGCAGGGGAG ACCCCGGCAC CAAGGGCAGC CCAGGCAGCG 
     2341 ATGGCCCCAA GGGGGAGAAG GGGGACCCTG GCCCTGAGGG CCCCCGCGGC CTGGCTGGAG 
     2401 AGGTTGGCAA CAAAGGAGCC AAGGGAGACC GAGGCTTGCC TGGACCCAGA GGCCCCCAGG 
     2461 GAGCTCTTGG GGAGCCCGGA AAGCAGGGAT CTCGGGGAGA CCCCGGTGAT GCAGGACCCC 
     2521 GTGGAGACTC AGGACAGCCA GGCCCCAAGG GAGACCCCGG CAGGCCTGGA TTCAGCTACC 
     2581 CAGGACCCCG AGGAGCACCC GGAGAAAAAG GCGAGCCCGG CCCACGCGGC CCCGAGGGAG 
     2641 GCCGAGGCGA CTTTGGCTTG AAAGGAGAAC CTGGGAGGAA AGGAGAGAAA GGAGAGCCTG 
     2701 CGGATCCTGG TCCCCCTGGT GAGCCAGGCC CTCGGGGGCC AAGAGGAGTC CCAGGACCCG 
     2761 AGGGTGAGCC CGGCCCCCCT GGAGACCCCG GTCTCACGGA GTGTGACGTC ATGACCTACG 
     2821 TGAGGGAGAC CTGCGGGTGC TGCGACTGTG AGAAGCGCTG TGGCGCCCTG GACGTGGTCT 
     2881 TCGTCATCGA CAGCTCCGAG AGCATTGGGT ACACCAACTT CACACTGGAG AAGAACTTCG 
     2941 TCATCAACGT GGTCAACAGG CTGGGTGCCA TCGCTAAGGA CCCCAAGTCC GAGACAGGGA 
     3001 CGCGTGTGGG CGTGGTGCAG TACAGCCACG AGGGCACCTT TGAGGCCATC CAGCTGGACG 
     3061 ACGAACATAT CGACTCCCTG TCGAGCTTCA AGGAGGCTGT CAAGAACCTC GAGTGGATTG 
     3121 CGGGCGGCAC CTGGACACCC TCAGCCCTCA AGTTTGCCTA CGACCGCCTC ATCAAGGAGA 
     3181 GCCGGCGCCA GAAGACACGT GTGTTTGCGG TGGTCATCAC GGACGGGCGC CACGACCCTC 
     3241 GGGACGATGA CCTCAACTTG CGGGCGCTGT GCGACCGCGA CGTCACAGTG ACGGCCATCG 
     3301 GCATCGGGGA CATGTTCCAC GAGAAGCACG AGAGTGAAAA CCTCTACTCC ATCGCCTGCG 
     3361 ACAAGCCACA GCAGGTGCGC AACATGACGC TGTTCTCCGA CCTGGTCGCT GAGAAGTTCA 
     3421 TCGATGACAT GGAGGACGTC CTCTGCCCGG ACCCTCAGAT CGTGTGCCCA GACCTTCCCT 
     3481 GCCAAACAGA TGCACCGTGG CCTGGCGGCG AGCCCCCGGT CACCTTCCTC CGCACGGAAG 
     3541 AGGGGCCGGA CGCCACCTTC CCCAGGACCA TTCCCCTGAT CCAACAGTTG CTAAACGCCA 
     3601 CGGAGCTCAC GCAGGACCCG GCCGCCTACT CCCAGCTGGT GGCCGTGCTG GTCTACACCG 
     3661 CCGAGCGGGC CAAGTTCGCC ACCGGGGTAG AGCGGCAGGA CTGGATGGAG CTGTTCATTG 
     3721 ACACCTTTAA GCTGGTGCAC AGGGACATCG TGGGGGACCC CGAGACCGCG CTGGCCCTCT 
     3781 GCAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3841 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3901 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3961 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4021 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4081 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4141 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4201 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4261 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4321 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4381 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4441 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4501 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4561 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4621 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4681 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4741 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4801 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4861 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4921 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4981 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5041 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5101 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5161 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5221 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5281 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5341 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5401 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5461 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5521 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5581 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5641 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5701 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5761 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5821 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5881 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5941 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6001 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6061 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6121 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6181 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6241 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6301 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6361 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6421 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6481 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6541 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6601 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6661 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6721 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6781 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6841 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6901 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6961 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7021 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7081 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7141 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7201 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7261 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7321 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7381 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7441 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7501 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7561 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7621 TAACAAAAAT TTAACGCGAA TTTT
//