LOCUS       RC218622                8574 bp    DNA     circular  SYN 
DEFINITION  FBLN2 (Myc-DDK-tagged)-Human fibulin 2 (FBLN2), transcript variant 1
ACCESSION   NM_001004019
VERSION     NM_001004019.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4731..4822 
                     /label="Myc-DDK"  
     misc_feature    4852..5437 
                     /label="PolyA signal"  
     misc_feature    4909..4928 
                     /label="Restriction Enzyme"  
     misc_feature    5713..6385 
                     /label="ColE1"  
     misc_feature    6815..7609 
                     /label="Kan/Neo"  
     misc_feature    7644..7946 
                     /label="SV40 ori"  
     misc_feature    8032..8509 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4713..4719 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4713 
                     /label="FBLN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCTGCTC TGGGAGCCTG CAGGAGCCTG GCTTGCTCTG GGCCTGGCCC 
     1081 TGGCCCTGGG CCCCAGCGTG GCCGCAGCTG CCCCTCGGCA GGACTGCACG GGCGTGGAGT 
     1141 GCCCGCCGCT GGAGAACTGC ATTGAGGAGG CGCTGGAGCC GGGTGCCTGC TGTGCCACGT 
     1201 GTGTGCAGCA GGGCTGCGCC TGCGAGGGCT ACCAGTACTA TGACTGCCTA CAGGGTGGCT 
     1261 TCGTGCGCGG CCGCGTGCCC GCCGGTCAGT CCTATTTTGT GGACTTCGGG AGCACTGAGT 
     1321 GCTCCTGCCC ACCAGGCGGC GGCAAGATCA GCTGCCAGTT CATGCTGTGC CCGGAGCTGC 
     1381 CGCCCAACTG CATCGAGGCT GTAGTGGTGG CTGACAGCTG CCCACAGTGC GGCCAGGTGG 
     1441 GCTGCGTCCA CGCGGGCCAC AAGTACGCCG CTGGCCACAC TGTTCACCTG CCGCCCTGCC 
     1501 GGGCCTGCCA CTGCCCTGAC GCCGGTGGAG AGCTCATCTG CTACCAGCTC CCCGGTTGCC 
     1561 ACGGGAACTT CTCAGATGCC GAGGAGGGTG ACCCCGAGCG ACACTACGAA GACCCCTACA 
     1621 GCTATGACCA GGAGGTGGCC GAGGTGGAAG CAGCAACAGC CCTGGGGGGT GAGGTCCAGG 
     1681 CGGGTGCAGT CCAGGCAGGC GCAGGGGGCC CCCCAGCTGC TCTGGGAGGT GGGAGTCAGC 
     1741 CACTGTCCAC CATCCAGGCA CCCCCCTGGC CAGCTGTCCT CCCCAGGCCC ACAGCGGCTG 
     1801 CTGCCCTGGG TCCCCCAGCC CCAGTGCAGG CCAAAGCTAG GAGAGTGACC GAGGACAGTG 
     1861 AGGAGGAAGA AGAGGAGGAG GAGGAGAGAG AGGAAATGGC TGTCACTGAG CAGCTGGCAG 
     1921 CAGGTGGCCA CAGGGGGCTG GATGGGCTGC CCACTACAGC CCCAGCTGGA CCCAGTCTTC 
     1981 CTATCCAGGA GGAGAGGGCA GAAGCTGGGG CAAGGGCAGA AGCTGGGGCA AGGCCTGAAG 
     2041 AGAACCTCAT CCTGGATGCC CAAGCCACGT CCCGCAGCAC TGGGCCGGAG GGCGTGACGC 
     2101 ATGCACCGAG CCTGGGCAAG GCTGCTCTCG TCCCAACTCA GGCCGTGCCT GGCTCTCCCA 
     2161 GGGACCCAGT CAAGCCCAGC CCCCACAACA TCCTGTCCAC ATCACTGCCT GATGCAGCCT 
     2221 GGATCCCACC CACCCGAGAA GTGCCCAGGA AGCCGCAAGT TCTGCCCCAT TCCCACGTGG 
     2281 AGGAGGACAC AGACCCCAAC TCTGTCCATT CTATCCCCAG AAGTAGCCCT GAAGGCTCCA 
     2341 CCAAGGACCT GATCGAGACT TGCTGCGCAG CCGGACAGCA GTGGGCCATT GACAATGACG 
     2401 AGTGCCTGGA GATCCCTGAG AGTGGCACTG AGGACAACGT CTGCAGGACA GCCCAGAGGC 
     2461 ACTGCTGTGT CTCCTACTTG CAGGAGAAGA GCTGCATGGC CGGCGTCCTG GGAGCCAAGG 
     2521 AGGGTGAGAC CTGTGGGGCT GAGGACAACG ACAGCTGCGG CATCTCCCTG TACAAGCAAT 
     2581 GCTGTGACTG CTGTGGCCTG GGCCTCCGCG TGCGGGCCGA GGGCCAGTCG TGTGAGTCCA 
     2641 ATCCTAACCT GGGCTATCCC TGCAATCATG TCATGCTCTC CTGCTGTGAG GGTGAAGAGC 
     2701 CTCTCATAGT ACCTGAGGTT CGCCGACCTC CAGAGCCCGC AGCTGCACCA CGGAGAGTTT 
     2761 CAGAGGCAGA GATGGCGGGC CGAGAGGCCC TGTCACTGGG CACAGAGGCC GAGCTGCCGA 
     2821 ACAGCCTGCC GGGCGATGAC CAGGATGAGT GCCTTCTCCT CCCGGGAGAG CTGTGCCAGC 
     2881 ACCTTTGCAT CAATACTGTG GGTTCTTACC ACTGTGCCTG CTTTCCTGGC TTCTCACTGC 
     2941 AGGACGATGG CCGCACTTGC CGCCCAGAGG GTCACCCTCC ACAGCCGGAA GCCCCACAGG 
     3001 AGCCTGCACT GAAGTCAGAA TTTTCCCAGG TGGCCTCTAA CACCATCCCG CTGCCACTGC 
     3061 CGCAGCCCAA TACCTGCAAA GACAATGGAC CCTGCAAGCA GGTGTGCAGC ACTGTTGGGG 
     3121 GCTCAGCCAT ATGCTCCTGT TTTCCCGGCT ATGCCATCAT GGCGGATGGC GTGTCCTGTG 
     3181 AAGACCAAGA CGAGTGCCTG ATGGGTGCTC ACGATTGTAG CCGGCGACAG TTCTGTGTGA 
     3241 ACACCCTGGG ATCCTTCTAC TGTGTCAACC ACACAGTGCT CTGTGCCGAT GGCTATATCC 
     3301 TCAATGCGCA CAGGAAGTGC GTGGACATCA ACGAGTGTGT GACGGACCTG CACACGTGCA 
     3361 GCCGGGGCGA GCACTGTGTG AACACACTGG GCTCCTTCCA CTGCTACAAG GCACTCACCT 
     3421 GTGAGCCAGG CTATGCCCTC AAGGATGGCG AGTGCGAAGA CGTGGATGAG TGTGCGATGG 
     3481 GCACGCACAC CTGCCAGCCG GGCTTCTTGT GCCAGAACAC CAAGGGCTCC TTCTACTGCC 
     3541 AGGCCAGGCA GCGCTGCATG GATGGCTTCC TGCAGGATCC TGAAGGCAAC TGTGTGGACA 
     3601 TCAACGAGTG CACGTCACTG TCCGAGCCAT GTCGGCCAGG CTTCAGCTGC ATCAACACGG 
     3661 TGGGCTCCTA CACATGCCAG AGGAACCCGC TGATCTGCGC GCGCGGCTAC CACGCCAGCG 
     3721 ATGATGGGAC CAAGTGTGTG GACGTGAATG AGTGTGAGAC AGGTGTGCAC CGCTGCGGTG 
     3781 AGGGCCAAGT GTGCCACAAC CTCCCTGGCT CCTACCGCTG TGACTGCAAA GCCGGCTTTC 
     3841 AGCGGGATGC CTTTGGCCGG GGCTGCATCG ACGTGAATGA GTGCTGGGCC TCGCCAGGCC 
     3901 GCCTGTGCCA GCACACGTGT GAGAACACAC TCGGCTCCTA CCGCTGTTCC TGCGCCTCCG 
     3961 GGTTCCTGCT AGCAGCGGAC GGCAAGCGCT GTGAAGACGT GAATGAGTGT GAGGCCCAGC 
     4021 GCTGCAGCCA GGAGTGTGCC AACATCTATG GCTCCTACCA GTGCTACTGC CGCCAGGGCT 
     4081 ACCAGCTGGC TGAGGATGGG CACACCTGCA CAGACATCGA CGAGTGTGCT CAAGGCGCCG 
     4141 GCATCCTCTG CACCTTCCGC TGTCTCAACG TGCCAGGGAG CTACCAGTGT GCATGCCCTG 
     4201 AGCAGGGCTA CACCATGACG GCCAACGGGA GGTCCTGCAA GGACGTGGAT GAGTGTGCAC 
     4261 TGGGTACCCA CAACTGTTCC GAGGCTGAGA CCTGCCACAA CATCCAGGGT AGCTTCCGCT 
     4321 GCCTGCGCTT CGAGTGTCCT CCCAACTATG TCCAAGTCTC CAAAACGAAG TGCGAGCGCA 
     4381 CCACGTGCCA TGACTTCCTG GAGTGCCAGA ACTCGCCAGC GCGCATCACG CACTACCAGC 
     4441 TCAACTTCCA GACGGGCCTC CTGGTGCCTG CGCATATCTT CCGCATTGGC CCCGCGCCAG 
     4501 CCTTCACGGG GGACACCATC GCCCTGAACA TCATCAAGGG CAATGAGGAG GGCTACTTTG 
     4561 GCACGCGCAG GCTCAATGCC TACACGGGTG TGGTCTACCT GCAGCGGGCC GTGCTGGAGC 
     4621 CCCGGGACTT TGCCCTGGAC GTGGAGATGA AGCTCTGGAG GCAGGGCTCC GTCACCACCT 
     4681 TCCTGGCCAA GATGCACATC TTCTTCACCA CCTTTGCCCT GACGCGTACG CGGCCGCTCG 
     4741 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4801 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4861 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4921 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4981 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5041 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5101 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5161 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5221 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5281 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5341 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5401 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5461 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5521 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5581 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5641 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5701 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5761 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5821 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5881 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5941 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6001 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6061 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6121 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6181 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6241 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6301 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6361 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6421 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6481 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6541 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6601 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6661 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6721 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6781 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6841 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6901 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6961 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7021 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7081 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7141 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7201 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7261 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7321 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7381 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7441 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7501 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7561 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7621 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7681 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7741 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7801 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7861 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7921 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7981 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8041 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8101 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8161 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8221 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8281 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8341 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8401 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8461 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8521 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//