LOCUS       RC218592                7524 bp    DNA     circular  SYN 
DEFINITION  PKP2 (Myc-DDK-tagged)-Human plakophilin 2 (PKP2), transcript variant 2b
ACCESSION   NM_004572
VERSION     NM_004572.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3681..3772 
                     /label="Myc-DDK"  
     misc_feature    3802..4387 
                     /label="PolyA signal"  
     misc_feature    3859..3878 
                     /label="Restriction Enzyme"  
     misc_feature    4663..5335 
                     /label="ColE1"  
     misc_feature    5765..6559 
                     /label="Kan/Neo"  
     misc_feature    6594..6896 
                     /label="SV40 ori"  
     misc_feature    6982..7459 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3663..3669 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3663 
                     /label="PKP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCCCCC GGCGCCCCAG CTGAGTACGG CTACATCCGG ACCGTCCTGG 
     1081 GCCAGCAGAT CCTGGGACAA CTGGACAGCT CCAGCCTGGC GCTGCCCTCC GAGGCCAAGC 
     1141 TGAAGCTGGC GGGGAGCAGC GGCCGCGGCG GCCAGACAGT CAAGAGCCTG CGGATCCAGG 
     1201 AGCAGGTGCA GCAGACCCTC GCCCGGAAGG GCCGCAGCTC CGTGGGCAAC GGAAATCTTC 
     1261 ACCGAACCAG CAGTGTTCCT GAGTATGTCT ACAACCTACA CTTGGTTGAA AATGATTTTG 
     1321 TTGGAGGCCG TTCCCCTGTT CCTAAAACCT ATGACATGCT AAAGGCTGGC ACAACTGCCA 
     1381 CTTATGAAGG TCGCTGGGGA AGAGGAACAG CACAGTACAG CTCCCAGAAG TCCGTGGAAG 
     1441 AAAGGTCCTT GAGGCATCCT CTGAGGAGAC TGGAGATTTC TCCTGACAGC AGCCCGGAGA 
     1501 GGGCTCACTA CACGCACAGC GATTACCAGT ACAGCCAGAG AAGCCAGGCT GGGCACACCC 
     1561 TGCACCACCA AGAAAGCAGG CGGGCCGCCC TCCTAGTGCC ACCGAGATAT GCTCGTTCCG 
     1621 AGATCGTGGG GGTCAGCCGT GCTGGCACCA CAAGCAGGCA GCGCCACTTT GACACATACC 
     1681 ACAGACAGTA CCAGCATGGC TCTGTTAGCG ACACCGTTTT TGACAGCATC CCTGCCAACC 
     1741 CGGCCCTGCT CACGTACCCC AGGCCAGGGA CCAGCCGCAG CATGGGCAAC CTCTTGGAGA 
     1801 AGGAGAACTA CCTGACGGCA GGGCTCACTG TCGGGCAGGT CAGGCCGCTG GTGCCCCTGC 
     1861 AGCCCGTCAC TCAGAACAGG GCTTCCAGGT CCTCCTGGCA TCAGAGCTCC TTCCACAGCA 
     1921 CCCGCACGCT GAGGGAAGCT GGGCCCAGTG TCGCCGTGGA TTCCAGCGGG AGGAGAGCGC 
     1981 ACTTGACTGT CGGCCAGGCG GCCGCAGGGG GAAGTGGGAA TCTGCTCACT GAGAGAAGCA 
     2041 CTTTCACTGA CTCCCAGCTG GGGAATGCAG ACATGGAGAT GACTCTGGAG CGAGCAGTGA 
     2101 GTATGCTCGA GGCAGACCAC ATGCTGCCAT CCAGGATTTC TGCTGCAGCT ACTTTCATAC 
     2161 AGCACGAGTG CTTCCAGAAA TCTGAAGCTC GGAAGAGGGT TAACCAGCTT CGTGGCATCC 
     2221 TCAAGCTTCT GCAGCTCCTA AAAGTTCAGA ATGAAGACGT TCAGCGAGCT GTGTGTGGGG 
     2281 CCTTGAGAAA CTTAGTATTT GAAGACAATG ACAACAAATT GGAGGTGGCT GAACTAAATG 
     2341 GGGTACCTCG GCTGCTCCAG GTGCTGAAGC AAACCAGAGA CTTGGAGACT AAAAAACAAA 
     2401 TAACAGACCA TACAGTCAAT TTAAGAAGTA GGAATGGCTG GCCGGGCGCG GTGGCTCACG 
     2461 CCTGTAATCC CAGCACTTTG GGAGGCCAAG GCGGGCGGAT CACGAGGTCA GGAGTTCGAG 
     2521 ACCAGCCTGA CCAACATGGT TTGCTGTGGA ATTTGTCATC TAATGACAAA CTCAAGAATC 
     2581 TCATGATAAC AGAAGCATTG CTTACGCTGA CGGAGAATAT CATCATCCCC TTTTCTGGGT 
     2641 GGCCTGAAGG AGACTACCCA AAAGCAAATG GTTTGCTCGA TTTTGACATA TTCTACAACG 
     2701 TCACTGGATG CCTAAGAAAC ATGAGTTCTG CTGGCGCTGA TGGGAGAAAA GCGATGAGAA 
     2761 GATGTGACGG ACTCATTGAC TCACTGGTCC ATTATGTCAG AGGAACCATT GCAGATTACC 
     2821 AGCCAGATGA CAAGGCCACG GAGAATTGTG TGTGCATTCT TCATAACCTC TCCTACCAGC 
     2881 TGGAGGCAGA GCTCCCAGAG AAATATTCCC AGAATATCTA TATTCAAAAC CGGAATATCC 
     2941 AGACTGACAA CAACAAAAGT ATTGGATGTT TTGGCAGTCG AAGCAGGAAA GTAAAAGAGC 
     3001 AATACCAGGA CGTGCCGATG CCGGAGGAAA AGAGCAACCC CAAGGGCGTG GAGTGGCTGT 
     3061 GGCATTCCAT TGTTATAAGG ATGTATCTGT CCTTGATCGC CAAAAGTGTC CGCAACTACA 
     3121 CACAAGAAGC ATCCTTAGGA GCTCTGCAGA ACCTCACGGC CGGAAGTGGA CCAATGCCGA 
     3181 CATCAGTGGC TCAGACAGTT GTCCAGAAGG AAAGTGGCCT GCAGCACACC CGAAAGATGC 
     3241 TGCATGTTGG TGACCCAAGT GTGAAAAAGA CAGCCATCTC GCTGCTGAGG AATCTGTCCC 
     3301 GGAATCTTTC TCTGCAGAAT GAAATTGCCA AAGAAACTCT CCCTGATTTG GTTTCCATCA 
     3361 TTCCTGACAC AGTCCCGAGT ACTGACCTTC TCATTGAAAC TACAGCCTCT GCCTGTTACA 
     3421 CATTGAACAA CATAATCCAA AACAGTTACC AGAATGCACG CGACCTTCTA AACACCGGGG 
     3481 GCATCCAGAA AATTATGGCC ATTAGTGCAG GCGATGCCTA TGCCTCCAAC AAAGCAAGTA 
     3541 AAGCTGCTTC CGTCCTTCTG TATTCTCTGT GGGCACACAC GGAACTGCAT CATGCCTACA 
     3601 AGAAGGCTCA GTTTAAGAAG ACAGATTTTG TCAACAGCCG GACTGCCAAA GCCTACCACT 
     3661 CCCTTAAAGA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3721 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3781 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3841 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3901 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3961 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4021 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4081 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4141 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4201 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4261 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4321 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4381 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4441 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4501 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4561 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4621 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4681 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4741 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4801 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4861 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4921 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4981 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5041 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5101 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5161 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5221 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5281 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5341 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5401 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5461 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5521 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5581 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5641 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5701 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5761 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5821 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5881 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5941 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6001 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6061 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6121 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6181 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6241 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6301 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6361 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6421 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6481 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6541 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6601 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6661 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6721 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6781 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6841 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6901 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6961 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7021 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7081 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7141 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7201 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7261 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7321 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7381 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7441 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7501 TAACAAAAAT TTAACGCGAA TTTT
//