LOCUS       RC218584                9447 bp    DNA     circular  SYN 
DEFINITION  TOPBP1 (Myc-DDK-tagged)-Human topoisomerase (DNA) II binding protein 1 (TOPBP1)
ACCESSION   NM_007027
VERSION     NM_007027.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5604..5695 
                     /label="Myc-DDK"  
     misc_feature    5725..6310 
                     /label="PolyA signal"  
     misc_feature    5782..5801 
                     /label="Restriction Enzyme"  
     misc_feature    6586..7258 
                     /label="ColE1"  
     misc_feature    7688..8482 
                     /label="Kan/Neo"  
     misc_feature    8517..8819 
                     /label="SV40 ori"  
     misc_feature    8905..9382 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5586..5592 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5586 
                     /label="TOPBP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCAGAAAT GACAAAGAAC CGTTTTTTGT GAAGTTTTTA AAGTCTTCAG 
     1081 ACAATTCCAA ATGTTTTTTT AAAGCTCTCG AGTCCATAAA AGAATTCCAA TCAGAAGAAT 
     1141 ATCTTCAGAT TATTACAGAA GAAGAGGCAT TGAAGATAAA GGAGAATGAT AGATCACTTT 
     1201 ATATCTGTGA CCCTTTTAGT GGCGTTGTCT TTGATCACCT CAAAAAGCTT GGCTGCAGAA 
     1261 TTGTTGGTCC TCAAGTAGTC ATATTTTGTA TGCACCACCA GCGATGTGTC CCAAGAGCCG 
     1321 AACATCCAGT TTATAATATG GTTATGTCTG ATGTAACCAT ATCTTGTACA AGTCTGGAAA 
     1381 AAGAAAAAAG GGAAGAAGTT CATAAATATG TACAAATGAT GGGCGGACGA GTATACAGAG 
     1441 ACCTTAATGT ATCAGTAACT CACCTTATTG CAGGAGAAGT TGGTAGCAAA AAATATTTAG 
     1501 TTGCTGCAAA CCTGAAGAAA CCTATTTTGC TTCCCTCTTG GATAAAAACA CTTTGGGAGA 
     1561 AGTCACAAGA GAAAAAAATA ACTAGATATA CTGATATAAA CATGGAAGAT TTCAAGTGTC 
     1621 CTATTTTTCT TGGTTGCATA ATCTGTGTGA CTGGCTTATG TGGCTTAGAC AGGAAAGAAG 
     1681 TTCAGCAACT CACAGTTAAG CATGGAGGTC AATACATGGG ACAATTGAAA ATGAATGAAT 
     1741 GTACACACCT CATTGTGCAA GAACCAAAAG GTCAGAAGTA TGAGTGTGCC AAGAGATGGA 
     1801 ATGTACACTG TGTGACCACA CAGTGGTTTT TTGACAGTAT TGAGAAAGGT TTTTGTCAGG 
     1861 ATGAATCCAT ATACAAGACA GAACCTAGAC CAGAAGCAAA GACTATGCCC AATTCTTCAA 
     1921 CTCCTACCAG CCAGATCAAC ACAATTGATA GTCGTACTCT TTCAGATGTC AGCAATATTT 
     1981 CCAACATAAA TGCAAGTTGC GTAAGTGAAT CAATATGTAA TTCACTTAAC AGCAAACTGG 
     2041 AGCCTACACT TGAAAATCTA GAAAATCTGG ATGTCAGTGC ATTTCAAGCA CCTGAAGATT 
     2101 TATTAGATGG TTGTCGGATA TATCTTTGCG GTTTTAGTGG CAGAAAGCTA GATAAACTGA 
     2161 GAAGACTTAT TAACAGTGGA GGTGGAGTTC GTTTTAACCA GCTAAATGAA GATGTAACTC 
     2221 ATGTTATTGT GGGAGATTAT GATGATGAAT TGAAGCAGTT TTGGAATAAA TCAGCCCACA 
     2281 GGCCTCATGT AGTGGGAGCA AAGTGGTTGC TAGAGTGTTT CAGTAAAGGT TATATGCTTT 
     2341 CTGAAGAACC ATATATCCAT GCTAATTACC AGCCAGTGGA AATTCCAGTT TCACATAAGC 
     2401 CTGAAAGTAA AGCAGCTCTT TTAAAAAAGA AGAACAGCAG CTTCTCTAAG AAAGACTTTG 
     2461 CTCCTAGTGA AAAGCATGAG CAAGCTGATG AAGATCTGCT CTCTCAATAT GAAAATGGTA 
     2521 GCTCCACAGT AGTTGAGGCT AAGACGTCTG AAGCCAGGCC CTTTAATGAT TCTACTCATG 
     2581 CTGAGCCCTT GAATGATTCT ACTCACATTT CTTTGCAAGA AGAAAACCAG TCTTCTGTCA 
     2641 GTCATTGTGT CCCTGATGTT TCTACAATTA CTGAAGAAGG CTTATTTAGC CAAAAGAGTT 
     2701 TCCTTGTTTT GGGTTTTAGT AATGAAAATG AATCTAACAT CGCAAACATC ATAAAAGAAA 
     2761 ATGCTGGGAA AATCATGTCC CTTCTGAGCA GAACTGTTGC GGATTATGCT GTGGTTCCTC 
     2821 TGCTGGGGTG TGAAGTGGAA GCCACTGTGG GAGAAGTTGT TACAAATACA TGGCTGGTTA 
     2881 CTTGCATAGA CTATCAGACT TTGTTTGATC CAAAGTCGAA TCCTCTCTTC ACACCAGTTC 
     2941 CAGTAATGAC AGGAATGACT CCTTTAGAGG ATTGTGTTAT TTCATTTAGC CAGTGTGCTG 
     3001 GAGCAGAAAA AGAGTCTTTA ACATTCCTAG CAAACCTCCT TGGAGCAAGT GTTCAAGAAT 
     3061 ACTTTGTTCG CAAATCCAAT GCAAAGAAAG GCATGTTTGC CAGTACTCAT CTTATACTGA 
     3121 AAGAACGTGG TGGCTCTAAA TATGAAGCTG CAAAGAAGTG GAATTTACCT GCCGTTACTA 
     3181 TAGCTTGGCT GTTGGAGACT GCTAGAACGG GAAAGAGAGC AGACGAAAGC CATTTTCTGA 
     3241 TTGAAAATTC AACTAAAGAA GAACGAAGTT TGGAAACAGA AATAACAAAT GGAATCAATC 
     3301 TAAATTCAGA TACTGCAGAG CATCCTGGCA CACGCCTGCA AACTCACAGA AAAACCGTCG 
     3361 TTACACCTTT AGATATGAAC CGCTTTCAGA GTAAAGCTTT CCGTGCTGTG GTCTCACAAC 
     3421 ATGCCAGACA GGTCGCAGCC TCCCCAGCAG TAGGACAACC ACTTCAGAAG GAGCCCTCGT 
     3481 TACACCTGGA TACACCATCA AAATTCCTGT CCAAGGACAA ACTCTTCAAG CCTTCCTTTG 
     3541 ATGTGAAGGA TGCACTTGCA GCCTTGGAAA CTCCAGGACG TCCCAGCCAA CAGAAAAGGA 
     3601 AACCGAGTAC GCCACTCTCA GAAGTTATTG TCAAAAACTT GCAACTTGCT TTGGCAAATA 
     3661 GCTCTCGAAA TGCTGTCGCT CTTTCTGCCA GCCCTCAACT GAAAGAGGCC CAGTCAGAGA 
     3721 AGGAAGAAGC CCCAAAGCCA CTTCACAAAG TAGTGGTATG TGTTAGTAAA AAACTCAGTA 
     3781 AGAAGCAGAG TGAACTAAAT GGGATCGCAG CCTCTCTAGG AGCAGATTAC AGGTGGAGTT 
     3841 TTGATGAAAC AGTGACTCAT TTCATCTATC AAGGGCGGCC AAATGACACT AATCGGGAGT 
     3901 ATAAATCTGT AAAAGAAAGA GGAGTACACA TTGTTTCCGA GCACTGGCTT TTAGATTGTG 
     3961 CCCAAGAGTG TAAACATCTT CCTGAATCTC TTTATCCACA TACTTATAAT CCCAAAATGA 
     4021 GCTTGGATAT CAGCGCAGTG CAAGATGGCC GGCTCTGTAA TAGTCGACTA CTCTCAGCTG 
     4081 TGTCTTCAAC AAAGGATGAT GAGCCAGATC CTTTGATTTT AGAAGAAAAT GATGTAGACA 
     4141 ATATGGCCAC CAATAATAAA GAGTCAGCAC CATCAAATGG AAGTGGAAAG AATGACTCTA 
     4201 AAGGAGTTCT GACACAGACC TTAGAGATGA GAGAGAACTT TCAGAAGCAG TTACAGGAGA 
     4261 TAATGTCTGC AACATCAATA GTGAAACCCC AAGGGCAGAG GACTTCCCTT TCAAGAAGTG 
     4321 GTTGTAACAG CGCATCTTCA ACCCCTGACA GCACTCGCTC TGCTCGCAGT GGACGAAGTA 
     4381 GAGTCCTAGA GGCACTGAGG CAGTCTCGTC AGACAGTACC TGATGTCAAC ACAGAGCCTT 
     4441 CCCAAAATGA ACAGATCATT TGGGATGACC CTACAGCAAG GGAGGAGAGA GCAAGGCTTG 
     4501 CCAGCAATTT GCAGTGGCCT AGTTGTCCCA CACAATACTC TGAGCTTCAG GTTGACATTC 
     4561 AAAACTTGGA GGATTCTCCT TTTCAAAAGC CTTTACATGA TTCAGAAATT GCTAAACAGG 
     4621 CTGTCTGTGA TCCTGGAAAC ATACGTGTGA CTGAAGCTCC CAAACACCCA ATCTCTGAAG 
     4681 AACTGGAAAC TCCCATAAAA GACAGCCACC TGATCCCTAC GCCTCAAGCC CCCAGTATTG 
     4741 CCTTTCCACT CGCCAACCCC CCTGTGGCTC CGCACCCTAG AGAAAAGATT ATAACGATAG 
     4801 AGGAGACTCA TGAAGAATTA AAAAAACAGT ACATATTTCA GTTATCATCT CTGAATCCTC 
     4861 AAGAACGTAT TGACTATTGT CATCTGATTG AGAAACTAGG TGGATTGGTG ATAGAAAAGC 
     4921 AGTGCTTTGA TCCCACCTGT ACACACATTG TTGTGGGACA TCCACTTCGA AACGAGAAGT 
     4981 ATTTAGCCTC AGTGGCAGCT GGGAAGTGGG TGCTTCATCG CTCCTACCTT GAAGCCTGCA 
     5041 GGACTGCTGG ACACTTCGTG CAGGAAGAAG ACTATGAATG GGGAAGTAGT TCCATACTTG 
     5101 ATGTTCTGAC TGGAATCAAT GTACAGCAAC GAAGACTAGC ACTTGCAGCA ATGAGATGGA 
     5161 GAAAAAAAAT CCAGCAAAGA CAAGAATCTG GCATTGTTGA GGGAGCATTT AGTGGGTGGA 
     5221 AGGTTATTTT ACATGTGGAT CAGTCTCGAG AAGCAGGCTT CAAACGCCTT CTTCAGTCAG 
     5281 GAGGAGCAAA GGTGCTACCT GGTCATTCTG TACCTTTATT TAAAGAGGCC ACACATCTTT 
     5341 TTTCTGACTT GAATAAACTG AAACCAGATG ACTCAGGAGT TAATATAGCA GAAGCTGCTG 
     5401 CCCAGAACGT GTACTGCTTG AGAACAGAAT ACATTGCTGA TTATCTCATG CAGGAATCAC 
     5461 CTCCTCATGT AGAAAATTAC TGTCTACCAG AAGCTATTTC ATTTATTCAG AATAATAAGG 
     5521 AACTTGGGAC TGGATTATCA CAAAAGAGGA AAGCTCCTAC AGAAAAAAAT AAAATCAAAC 
     5581 GACCTAGAGT ACACACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5641 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5701 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5761 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5821 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5881 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5941 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     6001 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     6061 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     6121 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6181 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6241 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6301 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6361 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6421 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6481 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6541 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6601 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6661 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6721 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6781 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6841 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6901 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6961 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     7021 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     7081 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     7141 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7201 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7261 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7321 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7381 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7441 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7501 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7561 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7621 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7681 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7741 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7801 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7861 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7921 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7981 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     8041 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     8101 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     8161 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8221 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8281 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8341 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8401 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8461 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8521 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8581 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8641 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8701 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8761 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8821 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8881 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8941 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     9001 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     9061 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     9121 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9181 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9241 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9301 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9361 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9421 ATTTAACAAA AATTTAACGC GAATTTT
//