LOCUS       RC218583                7623 bp    DNA     circular  SYN 
DEFINITION  CLCA1 (Myc-DDK-tagged)-Human chloride channel accessory 1 (CLCA1)
ACCESSION   NM_001285
VERSION     NM_001285.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3780..3871 
                     /label="Myc-DDK"  
     misc_feature    3901..4486 
                     /label="PolyA signal"  
     misc_feature    3958..3977 
                     /label="Restriction Enzyme"  
     misc_feature    4762..5434 
                     /label="ColE1"  
     misc_feature    5864..6658 
                     /label="Kan/Neo"  
     misc_feature    6693..6995 
                     /label="SV40 ori"  
     misc_feature    7081..7558 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3762..3768 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3762 
                     /label="CLCA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCCATTT AAGAGTTCTG TGTTCATCTT GATTCTTCAC CTTCTAGAAG 
     1081 GGGCCCTGAG TAATTCACTC ATTCAGCTGA ACAACAATGG CTATGAAGGC ATTGTCGTTG 
     1141 CAATCGACCC CAATGTGCCA GAAGATGAAA CACTCATTCA ACAAATAAAG GACATGGTGA 
     1201 CCCAGGCATC TCTGTATCTG TTTGAAGCTA CAGGAAAGCG ATTTTATTTC AAAAATGTTG 
     1261 CCATTTTGAT TCCTGAAACA TGGAAGACAA AGGCTGACTA TGTGAGACCA AAACTTGAGA 
     1321 CCTACAAAAA TGCTGATGTT CTGGTTGCTG AGTCTACTCC TCCAGGTAAT GATGAACCCT 
     1381 ACACTGAGCA GATGGGCAAC TGTGGAGAGA AGGGTGAAAG GATCCACCTC ACTCCTGATT 
     1441 TCATTGCAGG AAAAAAGTTA GCTGAATATG GACCACAAGG TAGGGCATTT GTCCATGAGT 
     1501 GGGCTCATCT ACGATGGGGA GTATTTGACG AGTACAATAA TGATGAGAAA TTCTACTTAT 
     1561 CCAATGGAAG AATACAAGCA GTAAGATGTT CAGCAGGTAT TACTGGTACA AATGTAGTAA 
     1621 AGAAGTGTCA GGGAGGCAGC TGTTACACCA AAAGATGCAC ATTCAATAAA GTAACAGGAC 
     1681 TCTATGAAAA AGGATGTGAG TTTGTTCTCC AATCCCGCCA GACGGAGAAG GCTTCTATAA 
     1741 TGTTTGCACA ACATGTTGAT TCTATAGTTG AATTCTGTAC AGAACAAAAC CACAACAAAG 
     1801 AAGCTCCAAA CAAGCAAAAT CAAAAATGCA ATCTCCGAAG CACATGGGAA GTGATCCGTG 
     1861 ATTCTGAGGA CTTTAAGAAA ACCACTCCTA TGACAACACA GCCACCAAAT CCCACCTTCT 
     1921 CATTGCTGCA GATTGGACAA AGAATTGTGT GTTTAGTCCT TGACAAATCT GGAAGCATGG 
     1981 CGACTGGTAA CCGCCTCAAT CGACTGAATC AAGCAGGCCA GCTTTTCCTG CTGCAGACAG 
     2041 TTGAGCTGGG GTCCTGGGTT GGGATGGTGA CATTTGACAG TGCTGCCCAT GTACAAAATG 
     2101 AACTCATACA GATAAACAGT GGCAGTGACA GGGACACACT CGCCAAAAGA TTACCTGCAG 
     2161 CAGCTTCAGG AGGGACGTCC ATCTGCAGCG GGCTTCGATC GGCATTTACT GTGATTAGGA 
     2221 AGAAATATCC AACTGATGGA TCTGAAATTG TGCTGCTGAC GGATGGGGAA GACAACACTA 
     2281 TAAGTGGGTG CTTTAACGAG GTCAAACAAA GTGGTGCCAT CATCCACACA GTCGCTTTGG 
     2341 GGCCCTCTGC AGCTCAAGAA CTAGAGGAGC TGTCCAAAAT GACAGGAGGT TTACAGACAT 
     2401 ATGCTTCAGA TCAAGTTCAG AACAATGGCC TCATTGATGC TTTTGGGGCC CTTTCATCAG 
     2461 GAAATGGAGC TGTCTCTCAG CGCTCCATCC AGCTTGAGAG TAAGGGATTA ACCCTCCAGA 
     2521 ACAGCCAGTG GATGAATGGC ACAGTGATCG TGGACAGCAC CGTGGGAAAG GACACTTTGT 
     2581 TTCTTATCAC CTGGACAATG CAGCCTCCCC AAATCCTTCT CTGGGATCCC AGTGGACAGA 
     2641 AGCAAGGTGG CTTTGTAGTG GACAAAAACA CCAAAATGGC CTACCTCCAA ATCCCAGGCA 
     2701 TTGCTAAGGT TGGCACTTGG AAATACAGTC TGCAAGCAAG CTCACAAACC TTGACCCTGA 
     2761 CTGTCACGTC CCGTGCGTCC AATGCTACCC TGCCTCCAAT TACAGTGACT TCCAAAACGA 
     2821 ACAAGGACAC CAGCAAATTC CCCAGCCCTC TGGTAGTTTA TGCAAATATT CGCCAAGGAG 
     2881 CCTCCCCAAT TCTCAGGGCC AGTGTCACAG CCCTGATTGA ATCAGTGAAT GGAAAAACAG 
     2941 TTACCTTGGA ACTACTGGAT AATGGAGCAG GTGCTGATGC TACTAAGGAT GACGGTGTCT 
     3001 ACTCAAGGTA TTTCACAACT TATGACACGA ATGGTAGATA CAGTGTAAAA GTGCGGGCTC 
     3061 TGGGAGGAGT TAACGCAGCC AGACGGAGAG TGATACCCCA GCAGAGTGGA GCACTGTACA 
     3121 TACCTGGCTG GATTGAGAAT GATGAAATAC AATGGAATCC ACCAAGACCT GAAATTAATA 
     3181 AGGATGATGT TCAACACAAG CAAGTGTGTT TCAGCAGAAC ATCCTCGGGA GGCTCATTTG 
     3241 TGGCTTCTGA TGTCCCAAAT GCTCCCATAC CTGATCTCTT CCCACCTGGC CAAATCACCG 
     3301 ACCTGAAGGC GGAAATTCAC GGGGGCAGTC TCATTAATCT GACTTGGACA GCTCCTGGGG 
     3361 ATGATTATGA CCATGGAACA GCTCACAAGT ATATCATTCG AATAAGTACA AGTATTCTTG 
     3421 ATCTCAGAGA CAAGTTCAAT GAATCTCTTC AAGTGAATAC TACTGCTCTC ATCCCAAAGG 
     3481 AAGCCAACTC TGAGGAAGTC TTTTTGTTTA AACCAGAAAA CATTACTTTT GAAAATGGCA 
     3541 CAGATCTTTT CATTGCTATT CAGGCTGTTG ATAAGGTCGA TCTGAAATCA GAAATATCCA 
     3601 ACATTGCACG AGTATCTTTG TTTATTCCTC CACAGACTCC GCCAGAGACA CCTAGTCCTG 
     3661 ATGAAACGTC TGCTCCTTGT CCTAATATTC ATATCAACAG CACCATTCCT GGCATTCACA 
     3721 TTTTAAAAAT TATGTGGAAG TGGATAGGAG AACTGCAGCT GTCAATAGCC ACGCGTACGC 
     3781 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3841 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3901 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3961 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4021 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4081 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4141 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4201 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4261 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4321 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4381 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4441 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4501 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4561 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4621 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4681 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4741 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4801 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4861 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4921 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4981 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5041 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5101 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5161 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5221 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5281 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5341 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5401 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5461 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5521 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5581 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5641 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5701 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5761 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5821 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5881 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5941 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6001 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6061 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6121 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6181 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6241 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6301 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6361 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6421 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6481 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6541 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6601 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6661 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6721 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6781 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6841 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6901 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6961 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7021 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7081 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7141 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7201 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7261 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7321 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7381 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7441 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7501 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7561 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7621 TTT
//