LOCUS       RC218506                9222 bp    DNA     circular  SYN 
DEFINITION  DCC (Myc-DDK-tagged)-Human deleted in colorectal carcinoma (DCC)
ACCESSION   NM_005215
VERSION     NM_005215.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5379..5470 
                     /label="Myc-DDK"  
     misc_feature    5500..6085 
                     /label="PolyA signal"  
     misc_feature    5557..5576 
                     /label="Restriction Enzyme"  
     misc_feature    6361..7033 
                     /label="ColE1"  
     misc_feature    7463..8257 
                     /label="Kan/Neo"  
     misc_feature    8292..8594 
                     /label="SV40 ori"  
     misc_feature    8680..9157 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5361..5367 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5361 
                     /label="DCC"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAATAGT CTTAGATGTG TTTGGGTACC CAAGCTGGCT TTTGTACTCT 
     1081 TCGGAGCTTC CTTGCTCAGC GCGCATCTTC AAGTAACCGG TTTTCAAATT AAAGCTTTCA 
     1141 CAGCACTGCG CTTCCTCTCA GAACCTTCTG ATGCCGTCAC AATGCGGGGA GGAAATGTCC 
     1201 TCCTCGACTG CTCCGCGGAG TCCGACCGAG GAGTTCCAGT GATCAAGTGG AAGAAAGATG 
     1261 GCATTCATCT GGCCTTGGGA ATGGATGAAA GGAAGCAGCA ACTTTCAAAT GGGTCTCTGC 
     1321 TGATACAAAA CATACTTCAT TCCAGACACC ACAAGCCAGA TGAGGGACTT TACCAATGTG 
     1381 AGGCATCTTT AGGAGATTCT GGCTCAATTA TTAGTCGGAC AGCAAAAGTT GCAGTAGCAG 
     1441 GACCACTGAG GTTCCTTTCA CAGACAGAAT CTGTCACAGC CTTCATGGGA GACACAGTGC 
     1501 TACTCAAGTG TGAAGTCATT GGGGAGCCCA TGCCAACAAT CCACTGGCAG AAGAACCAAC 
     1561 AAGACCTGAC TCCAATCCCA GGTGACTCCC GAGTGGTGGT CTTGCCCTCT GGAGCATTGC 
     1621 AGATCAGCCG ACTCCAACCG GGGGACATTG GAATTTACCG ATGCTCAGCT CGAAATCCAG 
     1681 CCAGCTCAAG AACAGGAAAT GAAGCAGAAG TCAGAATTTT ATCAGATCCA GGACTGCATA 
     1741 GACAGCTGTA TTTTCTGCAA AGACCATCCA ATGTAGTAGC CATTGAAGGA AAAGATGCTG 
     1801 TCCTGGAATG TTGTGTTTCT GGCTATCCTC CACCAAGTTT TACCTGGTTA CGAGGCGAGG 
     1861 AAGTCATCCA ACTCAGGTCT AAAAAGTATT CTTTATTGGG TGGAAGCAAC TTGCTTATCT 
     1921 CCAATGTGAC AGATGATGAC AGTGGAATGT ATACCTGTGT TGTCACATAT AAAAATGAGA 
     1981 ATATTAGTGC CTCTGCAGAG CTCACAGTCT TGGTTCCGCC ATGGTTTTTA AATCATCCTT 
     2041 CCAACCTGTA TGCCTATGAA AGCATGGATA TTGAGTTTGA ATGTACAGTC TCTGGAAAGC 
     2101 CTGTGCCCAC TGTGAATTGG ATGAAGAATG GAGATGTGGT CATTCCTAGT GATTATTTTC 
     2161 AGATAGTGGG AGGAAGCAAC TTACGGATAC TTGGGGTGGT GAAGTCAGAT GAAGGCTTTT 
     2221 ATCAATGTGT GGCTGAAAAT GAGGCTGGAA ATGCCCAGAC CAGTGCACAG CTCATTGTCC 
     2281 CTAAGCCTGC TATCCCAAGC TCCAGTGTCC TCCCTTCGGC TCCCAGAGAT GTGGTCCCTG 
     2341 TCTTGGTTTC CAGCCGATTT GTCCGTCTCA GCTGGCGCCC ACCTGCAGAA GCGAAAGGGA 
     2401 ACATTCAAAC TTTCACGGTC TTTTTCTCCA GAGAAGGTGA CAACAGGGAA CGAGCATTGA 
     2461 ATACAACACA GCCTGGGTCC CTTCAGCTCA CTGTGGGAAA CCTGAAGCCA GAAGCCATGT 
     2521 ACACCTTTCG AGTTGTGGCT TACAATGAAT GGGGACCGGG AGAGAGTTCT CAACCCATCA 
     2581 AGGTGGCCAC ACAGCCTGAG TTGCAAGTTC CAGGGCCAGT AGAAAACCTG CAAGCTGTAT 
     2641 CTACCTCACC TACCTCAATT CTTATTACCT GGGAACCCCC TGCCTATGCA AACGGTCCAG 
     2701 TCCAAGGTTA CAGATTGTTC TGCACTGAGG TGTCCACAGG AAAAGAACAG AATATAGAGG 
     2761 TTGATGGACT ATCTTATAAA CTGGAAGGCC TGAAAAAATT CACCGAATAT AGTCTTCGAT 
     2821 TCTTAGCTTA TAATCGCTAT GGTCCGGGCG TCTCTACTGA TGATATAACA GTGGTTACAC 
     2881 TTTCTGACGT GCCAAGTGCC CCGCCTCAGA ACGTCTCCCT GGAAGTGGTC AATTCAAGAA 
     2941 GTATCAAAGT TAGCTGGCTG CCTCCTCCAT CAGGAACACA AAATGGATTT ATTACCGGCT 
     3001 ATAAAATTCG ACACAGAAAG ACGACCCGCA GGGGTGAGAT GGAAACACTG GAGCCAAACA 
     3061 ACCTCTGGTA CCTATTCACA GGACTGGAGA AAGGAAGTCA GTACAGTTTC CAGGTGTCAG 
     3121 CCATGACAGT CAATGGTACT GGACCACCTT CCAACTGGTA TACTGCAGAG ACTCCAGAGA 
     3181 ATGATCTAGA TGAATCTCAA GTTCCTGATC AACCAAGCTC TCTTCATGTG AGGCCCCAGA 
     3241 CTAACTGCAT CATCATGAGT TGGACTCCTC CCTTGAACCC AAACATCGTG GTGCGAGGTT 
     3301 ATATTATCGG TTATGGCGTT GGGAGCCCTT ACGCTGAGAC AGTGCGTGTG GACAGCAAGC 
     3361 AGCGATATTA TTCCATTGAG AGGTTAGAGT CAAGTTCCCA TTATGTAATC TCCCTAAAAG 
     3421 CTTTTAACAA TGCCGGAGAA GGAGTTCCTC TTTATGAAAG TGCCACCACC AGGTCTATAA 
     3481 CCGATCCCAC TGACCCAGTT GATTATTATC CTTTGCTTGA TGATTTCCCC ACCTCGGTCC 
     3541 CAGATCTCTC CACCCCCATG CTCCCACCAG TAGGTGTACA GGCTGTGGCT CTTACCCATG 
     3601 ATGCTGTGAG GGTCAGCTGG GCAGACAACT CTGTCCCTAA GAACCAAAAG ACGTCTGAGG 
     3661 TGCGACTTTA CACCGTCCGG TGGAGAACCA GCTTTTCTGC AAGTGCAAAA TACAAGTCAG 
     3721 AAGACACAAC ATCTCTAAGT TACACAGCAA CAGGCCTCAA ACCAAACACA ATGTATGAAT 
     3781 TCTCGGTCAT GGTAACAAAA AACAGAAGGT CCAGTACTTG GAGCATGACT GCACATGCCA 
     3841 CCACGTATGA AGCAGCCCCC ACCTCTGCTC CCAAGGACTT GACAGTCATT ACTAGGGAAG 
     3901 GGAAGCCTCG TGCCGTCATT GTGAGTTGGC AGCCTCCCTT GGAAGCCAAT GGGAAAATTA 
     3961 CTGCTTACAT CTTATTTTAT ACCTTGGACA AGAACATCCC AATTGATGAC TGGATTATGG 
     4021 AAACAATCAG TGGTGATAGG CTTACTCATC AAATCATGGA TCTCAACCTT GATACTATGT 
     4081 ATTACTTTCG AATTCAAGCA CGAAATTCAA AAGGAGTGGG GCCACTCTCT GATCCTATCC 
     4141 TCTTCAGGAC TCTGAAAGTG GAACACCCTG ACAAAATGGC TAATGACCAA GGTCGTCATG 
     4201 GAGATGGAGG TTATTGGCCA GTTGATACTA ATTTGATTGA TAGAAGCACC CTAAATGAGC 
     4261 CGCCAATTGG ACAAATGCAC CCCCCGCATG GCAGTGTCAC TCCTCAGAAG AACAGCAACC 
     4321 TGCTTGTGAT CATTGTGGTC ACCGTTGGTG TCATCACAGT GCTGGTAGTG GTCATCGTGG 
     4381 CTGTGATTTG CACCCGACGC TCTTCAGCCC AGCAGAGAAA GAAACGGGCC ACCCACAGTG 
     4441 CTGGCAAAAG GAAGGGCAGC CAGAAGGACC TCCGACCCCC TGATCTTTGG ATCCATCATG 
     4501 AAGAAATGGA GATGAAAAAT ATTGAAAAGC CATCTGGCAC TGACCCTGCA GGAAGGGACT 
     4561 CTCCCATCCA AAGTTGCCAA GACCTCACAC CAGTCAGCCA CAGCCAGTCA GAAACCCAAC 
     4621 TGGGAAGCAA AAGCACCTCT CATTCAGGTC AAGACACTGA GGAAGCAGGG AGCTCTATGT 
     4681 CCACTCTGGA GAGGTCGCTG GCTGCACGCC GAGCCCCCCG GGCCAAGCTC ATGATTCCCA 
     4741 TGGATGCCCA GTCCAACAAT CCTGCTGTCG TGAGCGCCAT CCCGGTGCCA ACGCTAGAAA 
     4801 GTGCCCAGTA CCCAGGAATC CTCCCGTCTC CCACCTGTGG ATATCCCCAC CCGCAGTTCA 
     4861 CTCTCCGGCC TGTGCCATTC CCAACACTCT CAGTGGACCG AGGTTTCGGA GCAGGAAGAA 
     4921 GTCAGTCAGT GAGTGAAGGA CCAACTACCC AACAACCACC TATGCTGCCC CCATCTCAGC 
     4981 CTGAGCATTC TAGCAGCGAG GAGGCACCAA GCAGAACCAT CCCCACAGCT TGTGTTCGAC 
     5041 CAACTCACCC ACTCCGCAGC TTTGCTAATC CTTTGCTACC TCCACCAATG AGTGCAATAG 
     5101 AACCGAAAGT CCCTTACACA CCACTTTTGT CTCAGCCAGG GCCCACTCTT CCTAAGACCC 
     5161 ATGTGAAAAC AGCCTCCCTT GGGTTGGCTG GAAAAGCAAG ATCCCCTTTG CTTCCTGTGT 
     5221 CTGTGCCAAC AGCCCCTGAA GTGTCTGAGG AGAGCCACAA ACCAACAGAG GATTCAGCCA 
     5281 ATGTGTATGA ACAGGATGAT CTGAGTGAAC AAATGGCAAG TTTGGAAGGA CTCATGAAGC 
     5341 AGCTTAATGC CATCACAGGC TCAGCCTTTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5401 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5461 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5521 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5581 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5641 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5701 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5761 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5821 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5881 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5941 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     6001 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     6061 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6121 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6181 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6241 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6301 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6361 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6421 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6481 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6541 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6601 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6661 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6721 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6781 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6841 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6901 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6961 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     7021 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     7081 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7141 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7201 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7261 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7321 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7381 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7441 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7501 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7561 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7621 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7681 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7741 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7801 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7861 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7921 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7981 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     8041 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     8101 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8161 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8221 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8281 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8341 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8401 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8461 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8521 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8581 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8641 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8701 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8761 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8821 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8881 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8941 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     9001 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     9061 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9121 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9181 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//