LOCUS       RC218458                8106 bp    DNA     circular  SYN 
DEFINITION  POTEE (Myc-DDK-tagged)-Human POTE ankyrin domain family, member E (POTEE)
ACCESSION   NM_001083538
VERSION     NM_001083538.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4263..4354 
                     /label="Myc-DDK"  
     misc_feature    4384..4969 
                     /label="PolyA signal"  
     misc_feature    4441..4460 
                     /label="Restriction Enzyme"  
     misc_feature    5245..5917 
                     /label="ColE1"  
     misc_feature    6347..7141 
                     /label="Kan/Neo"  
     misc_feature    7176..7478 
                     /label="SV40 ori"  
     misc_feature    7564..8041 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4245..4251 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4245 
                     /label="POTEE"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGTTGAG GTTGATTCCA TGCCGGCTGC CTCTTCTGTG AAGAAGCCAT 
     1081 TTGGTCTCAG GAGCAAGATG GGCAAGTGGT GCTGCCGTTG CTTCCCCTGC TACAGGGAGA 
     1141 GCGGCAAGAG CAACGTGGGC ACTTCTGGAG ACCACGACGA CTCTGCTATG AAGACACTCA 
     1201 GGAGCAAGAT GGGCAAGTGG TGCCACCACT GCTTCCCCTG CTGCAGGGGG AGTGGCAAGA 
     1261 GCAACGTGGG CGCTTCTGGA GACCACGACG ACTCTGCTAT GAAGACACTC AGGAACAAGA 
     1321 TGGGGAAGTG GTGCTGCCAC TGCTTCCCCT GCTGCAGGGG GAGCGGCAAG AGCAAGGTGG 
     1381 GCGCTTGGGG AGACTACGAT GACAGCGCCT TCATGGAGCC CAGGTACCAC GTCCGTGGAG 
     1441 AAGATCTGGA CAAGCTCCAC AGAGCTGCCT GGTGGGGTAA AGTCCCCAGA AAGGATCTCA 
     1501 TCGTCATGCT CAGGGACACT GACGTGAACA AGAAGGACAA GCAAAAGAGG ACTGCTCTAC 
     1561 ATCTGGCCTC TGCCAATGGG AATTCAGAAG TAGTAAAACT CCTGCTGGAC AGACGATGTC 
     1621 AACTTAATGT CCTTGACAAC AAAAAGAGGA CAGCTCTGAT AAAGGCCGTA CAATGCCAGG 
     1681 AAGATGAATG TGCGTTAATG TTGCTGGAAC ATGGCACTGA TCCAAATATT CCAGATGAGT 
     1741 ATGGAAATAC CACTCTGCAC TACGCTATCT ATAATGAAGA TAAATTAATG GCCAAAGCAC 
     1801 TGCTCTTATA TGGTGCTGAT ATCGAATCAA AAAACAAGCA TGGCCTCACA CCACTGTTAC 
     1861 TTGGTGTACA TGAGCAAAAA CAGCAAGTCG TGAAATTTTT AATCAAGAAA AAAGCGAATT 
     1921 TAAATGCACT GGATAGATAT GGAAGGACTG CTCTCATACT TGCTGTATGT TGTGGATCAG 
     1981 CAAGTATAGT CAGCCTTCTA CTTGAGCAAA ATATTGATGT ATCTTCTCAA GATCTATCTG 
     2041 GACAGACGGC CAGAGAGTAT GCTGTTTCTA GTCATCATCA TGTAATTTGC CAGTTACTTT 
     2101 CTGACTACAA AGAAAAACAG ATGCTAAAAA TCTCTTCTGA AAACAGCAAT CCAGAACAAG 
     2161 AGTTAAAGCT GACATCAGAG GAAGAGTCAC AAAGGTTCAA AGGCAGTGAA AATAGCCAGC 
     2221 CAGAGAAAAT GTCTCAAGAA CTAGAAATAA ATAAGGATGG TGATAGAGAG GTTGAAGAAG 
     2281 AAATGAAGAA GCATGAAAGT AATAATGTGG GATTACTAGA AAACCTGACT AATGGTGTCA 
     2341 CTGCTGGCAA TGGTGATAAT GGATTAATTC CTCAAAGGAA GAGCAGAACA CCTGAAAATC 
     2401 AGCAATTTCC TGACAACGAA AGTGAAGAGT ATCACAGAAT TTGTGAATTA CTTTCTGACT 
     2461 ACAAAGAAAA GCAGATGCCA AAATACTCTT CTGAAAACAG CAACCCAGAA CAAGACTTAA 
     2521 AGCTGACATC AGAGGAAGAG TCACAAAGGC TTAAAGGCAG TGAAAATGGC CAGCCAGAGA 
     2581 AAAGATCTCA AGAACCAGAA ATAAATAAGG ATGGTGATAG AGAGCTAGAA AATTTTATGG 
     2641 CTATCGAAGA AATGAAGAAG CACGGAAGTA CTCATGTCGG ATTCCCAGAA AACCTGACTA 
     2701 ATGGTGCCAC TGCTGGCAAT GGTGATGATG GATTAATTCC TCCAAGGAAG AGCAGAACAC 
     2761 CTGAAAGCCA GCAATTTCCT GACACTGAGA ATGAAGAGTA TCACAGTGAC GAACAAAATG 
     2821 ATACTCAGAA GCAATTTTGT GAAGAACAGA ACACTGGAAT ATTACACGAT GAGATTCTGA 
     2881 TTCATGAAGA AAAGCAGATA GAAGTGGTTG AAAAAATGAA TTCTGAGCTT TCTCTTAGTT 
     2941 GTAAGAAAGA AAAAGACGTC TTGCATGAAA ATAGTACGTT GCGGGAAGAA ATTGCCATGC 
     3001 TAAGACTGGA GCTAGACACA ATGAAACATC AGAGCCAGCT AAGAGAAAAG AAATATTTGG 
     3061 AGGATATTGA AAGTGTGAAA AAAAAGAATG ATAATCTTTT AAAGGCTCTA CAATTGAATG 
     3121 AGCTCACCAT GGATGATGAT ACCGCCGTGC TCGTCATTGA CAACGGCTCT GGCATGTGCA 
     3181 AGGCCGGCTT TGCGGGCGAC GATGCCCCCC GGGCTGTCTT CCCTTCCATC GTGGGGCGCC 
     3241 CCAGGCAGCA GGGCATGATG GGGGGCATGC ATCAGAAAGA GTCCTATGTG GGCAAGGAGG 
     3301 CCCAGAGCAA GAGAGGCATC CTGACCCTGA AGTACCCCAT GGAACACGGC ATCATCACCA 
     3361 ACTGGGATGA CATGGAGAAG ATCTGGCACC ACACCTTCTA CAACGAGCTG CGTGTGGCTC 
     3421 CCGAGGAGCA CCCCATCCTG CTGACCGAGG CCCCCCTGAA CCCCAAGGCC AACCGCGAGA 
     3481 AGATGACCCA GATCATGTTT GAGACCTTCA ACACCCCAGC CATGTACGTG GCCATCCAGG 
     3541 CCGTGCCGTC CCTGTACACC TCTGGCCGTA CTACTGGCAT CGTGATGGAC TCTGGTGACG 
     3601 GGGTCACCCA CACTGTGCCC ATCTATGAGG GGAATGCCCT CCCCCATGCC ACCCTGCGCC 
     3661 TAGACCTGGC TGGGCGGGAA CTGCCTGACT ACCTCATGAA GATCCTCACC GAGCGTGGCT 
     3721 ATAGGTTCAC CACCATGGCC GAGCGGGAAA TCGTGCGTGA CATCAAAGAG AAGCTGTGCT 
     3781 ATGTTGCCCT GGACTTCGAG CAGGAGATGG CCACGGCGGC CTCCAGCTCC TCCCTAGAGA 
     3841 AGAGCTACGA GCTGCCCGAT GGCCAGGTCA TCACCATCGG CAACGAGCGG TTCCGCTGCC 
     3901 CCGAGGCGCT CTTCCAGCCT TGCTTCCTGG GCATGGAATC CTGTGGCATC CATGAAACTA 
     3961 CCTTCAACTC CATCATGAAG TCTGATGTGG ACATCCGCAA AGACCTGTAC ACCAACACAG 
     4021 TGCTGTCTGG CGGCACCACC ATGTACCCTG GCATGGCCCA CAGAATGCAG AAGGAGATCG 
     4081 CTGCCCTGGC GCCTAGCATG ATGAAGATCA GGATCATTGC TCCTCCCAAG CGCAAGTACT 
     4141 CCGTGTGGGT CGGTGGCTCC ATCCTGGCCT CGCTGTCCAC CTTCCAGCAG ATGTGGATCA 
     4201 GCAAGCAGGA GTATGATGAG TCAGGCCCCT CCATTGTCCA CCGCAAATGC TTCACGCGTA 
     4261 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4321 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4381 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4441 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4501 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4561 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4621 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4681 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4741 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4801 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4861 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4921 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4981 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5041 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5101 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5161 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5221 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5281 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5341 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5401 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5461 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5521 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5581 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5641 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5701 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5761 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5821 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5881 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5941 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6001 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6061 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6121 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6181 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6241 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6301 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6361 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6421 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6481 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6541 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6601 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6661 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6721 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6781 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6841 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6901 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6961 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7021 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7081 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7141 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7201 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7261 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7321 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7381 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7441 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7501 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7561 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7621 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7681 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7741 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7801 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7861 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7921 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7981 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8041 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8101 AATTTT
//