LOCUS       RC218429                6702 bp    DNA     circular  SYN 
DEFINITION  NTN1 (Myc-DDK-tagged)-Human netrin 1 (NTN1)
ACCESSION   NM_004822
VERSION     NM_004822.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2857..2948 
                     /label="Myc-DDK"  
     misc_feature    2978..3563 
                     /label="PolyA signal"  
     misc_feature    3035..3054 
                     /label="Restriction Enzyme"  
     misc_feature    3839..4511 
                     /label="ColE1"  
     misc_feature    4941..5735 
                     /label="Kan/Neo"  
     misc_feature    5770..6072 
                     /label="SV40 ori"  
     misc_feature    6158..6635 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2832..2839 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2832 
                     /label="NTN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGCGCGCA GTGTGGGAGG CGCTGGCGGC GCTGGCGGCG GTGGCGTGCC 
     1081 TGGTGGGCGC GGTGCGCGGC GGGCCCGGGC TCAGCATGTT CGCGGGCCAG GCGGCGCAGC 
     1141 CCGATCCCTG CTCGGACGAG AACGGCCACC CGCGCCGCTG CATCCCGGAC TTTGTCAATG 
     1201 CGGCCTTCGG CAAGGACGTG CGCGTGTCCA GCACCTGCGC CCGGCCCCCG GCGCGCTACT 
     1261 GCGTGGTGAG CGAGCGCGGC GAGGAGCGGC TGCGCTCGTG CCACCTCTGC AACGCGTCCG 
     1321 ACCCCAAGAA GGCGCACCCG CCCGCCTTCC TCACCGACCT CAACAACCCG CACAACCTGA 
     1381 CGTGCTGGCA GTCCGAGAAC TACCTGCAGT TCCCGCACAA CGTCACGCTC ACACTGTCCC 
     1441 TCGGCAAGAA GTTCGAAGTG ACCTACGTGA GCCTGCAGTT CTGCTCGCCG CGGCCCGAGT 
     1501 CCATGGCCAT CTACAAGTCC ATGGACTACG GGCGCACGTG GGTGCCCTTC CAGTTCTACT 
     1561 CCACGCAGTG CCGCAAGATG TACAACCGGC CGCACCGCGC GCCCATCACC AAGCAGAACG 
     1621 AGCAGGAGGC CGTGTGCACC GACTCGCACA CCGACATGCG CCCGCTCTCG GGCGGCCTCA 
     1681 TCGCCTTCAG CACGCTGGAC GGGCGGCCCT CGGCGCACGA CTTCGACAAC TCGCCCGTGC 
     1741 TGCAGGACTG GGTCACGGCC ACAGACATCC GCGTGGCCTT CAGCCGCCTG CACACGTTCG 
     1801 GCGACGAGAA CGAGGACGAC TCGGAGCTGG CGCGCGACTC GTACTTCTAC GCGGTGTCCG 
     1861 ACCTGCAGGT GGGCGGCCGG TGCAAGTGCA ACGGCCACGC GGCCCGCTGC GTGCGCGACC 
     1921 GCACCGACAG CCTGGTGTGC GACTGCAGGC ACAACACGGC CGGCCCGGAG TGCGACCGCT 
     1981 GCAAGCCCTT CCACTACGAC CGGCCCTGGC AGCGCGCCAC AGCCCGCGAA GCCAACGAGT 
     2041 GCGTGGCCTG TAACTGCAAC CTGCATGCCC GGCGCTGCCG CTTCAACATG GAGCTCTACA 
     2101 AGCTTTCGGG GCGCAAGAGC GGAGGTGTCT GCCTCAACTG TCGCCACAAC ACCGCCGGCC 
     2161 GCCACTGCCA TTACTGCAAG GAGGGCTACT ACCGCGACAT GGGCAAGCCC ATCACCCACC 
     2221 GGAAGGCCTG CAAAGCCTGT GATTGCCACC CTGTGGGTGC TGCTGGCAAA ACCTGCAACC 
     2281 AAACCACCGG CCAGTGTCCC TGCAAGGACG GCGTGACGGG TATCACCTGC AACCGCTGCG 
     2341 CCAAAGGCTA CCAGCAGAGC CGCTCTCCCA TCGCCCCCTG CATAAAGATC CCTGTAGCGC 
     2401 CGCCGACGAC TGCAGCCAGC AGCGTGGAGG AGCCTGAAGA CTGCGATTCC TACTGCAAGG 
     2461 CCTCCAAGGG GAAGCTGAAG ATTAACATGA AAAAGTACTG CAAGAAGGAC TATGCCGTCC 
     2521 AGATCCACAT CCTGAAGGCG GACAAGGCGG GGGACTGGTG GAAGTTCACG GTGAACATCA 
     2581 TCTCCGTGTA TAAGCAGGGC ACGAGCCGCA TCCGCCGCGG TGACCAGAGC CTGTGGATCC 
     2641 GCTCGCGGGA CATCGCCTGC AAGTGTCCCA AAATCAAGCC CCTCAAGAAG TACCTGCTGC 
     2701 TGGGCAACGC GGAGGACTCT CCGGACCAGA GCGGCATCGT GGCCGATAAA AGCAGCCTGG 
     2761 TGATCCAGTG GCGGGACACG TGGGCGCGGC GGCTGCGCAA GTTCCAGCAG CGTGAGAAGA 
     2821 AGGGCAAGTG CAAGAAGGCC AGCGGACCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     2881 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     2941 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3001 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3061 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3121 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3181 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3241 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3301 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3361 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3421 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3481 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3541 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3601 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3661 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     3721 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     3781 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     3841 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     3901 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     3961 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4021 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4081 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4141 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4201 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4261 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4321 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4381 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4441 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4501 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4561 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4621 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4681 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     4741 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     4801 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     4861 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     4921 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     4981 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5041 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5101 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5161 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5221 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5281 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5341 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5401 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5461 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5521 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5581 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5641 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     5701 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     5761 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     5821 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     5881 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     5941 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6001 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6061 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6121 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6181 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6241 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6301 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6361 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6421 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6481 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6541 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6601 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6661 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//