LOCUS       RC218393                6642 bp    DNA     circular  SYN 
DEFINITION  KLHL3 (Myc-DDK-tagged)-Human kelch-like 3 (Drosophila) (KLHL3)
ACCESSION   NM_017415
VERSION     NM_017415.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2799..2890 
                     /label="Myc-DDK"  
     misc_feature    2920..3505 
                     /label="PolyA signal"  
     misc_feature    2977..2996 
                     /label="Restriction Enzyme"  
     misc_feature    3781..4453 
                     /label="ColE1"  
     misc_feature    4883..5677 
                     /label="Kan/Neo"  
     misc_feature    5712..6014 
                     /label="SV40 ori"  
     misc_feature    6100..6577 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2781..2787 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2781 
                     /label="KLHL3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGGTGAA AGTGTCAAGC TGAGCTCCCA GACTCTGATA CAGGCTGGGG 
     1081 ATGATGAGAA GAACCAGAGG ACGATCACTG TCAACCCTGC CCACATGGGG AAAGCATTCA 
     1141 AGGTTATGAA TGAACTGCGG AGTAAACAGC TGTTGTGTGA CGTGATGATT GTGGCAGAAG 
     1201 ATGTCGAGAT AGAAGCCCAC CGTGTGGTCC TGGCAGCCTG CAGCCCCTAC TTCTGTGCGA 
     1261 TGTTCACAGG TGACATGTCT GAGAGTAAAG CCAAAAAGAT AGAAATCAAG GACGTGGATG 
     1321 GGCAGACGCT GAGTAAGCTG ATTGACTACA TCTATACTGC TGAAATCGAG GTGACTGAAG 
     1381 AGAATGTCCA GGTGCTGCTC CCGGCAGCCA GCTTGCTGCA GCTCATGGAT GTTCGGCAGA 
     1441 ACTGCTGTGA CTTCCTGCAG TCTCAGTTGC ATCCCACCAA TTGCCTGGGC ATCCGTGCAT 
     1501 TTGCAGATGT ACACACCTGC ACTGACCTTC TGCAGCAGGC CAATGCCTAC GCAGAGCAGC 
     1561 ACTTTCCAGA GGTGATGCTA GGAGAAGAAT TTCTTAGCCT GAGTCTGGAC CAGGTGTGCA 
     1621 GCTTGATATC CAGCGACAAG CTGACCGTTT CTTCAGAAGA GAAGGTGTTT GAAGCTGTGA 
     1681 TCTCATGGAT CAATTATGAG AAAGAAACCC GTTTAGAGCA CATGGCAAAG CTGATGGAAC 
     1741 ATGTCCGACT TCCTCTCTTA CCTAGGGACT ACCTAGTCCA AACGGTTGAA GAAGAAGCTT 
     1801 TGATAAAGAA TAACAACACC TGTAAAGACT TCCTCATTGA GGCCATGAAA TACCATCTCC 
     1861 TCCCTCTGGA TCAGAGACTA TTGATTAAGA ACCCAAGGAC CAAGCCCAGG ACTCCAGTCA 
     1921 GCCTTCCCAA GGTCATGATT GTGGTTGGCG GCCAGGCACC CAAGGCAATC CGCAGTGTGG 
     1981 AGTGCTATGA TTTCGAGGAG GACCGGTGGG ATCAGATTGC TGAGCTTCCT TCCAGAAGAT 
     2041 GCAGAGCAGG TGTGGTGTTC ATGGCTGGCC ACGTGTATGC CGTGGGAGGG TTTAATGGCT 
     2101 CACTGCGGGT GCGGACAGTG GATGTGTATG ACGGCGTGAA GGACCAGTGG ACGTCCATTG 
     2161 CCAGCATGCA GGAGCGCCGG AGCACACTGG GCGCAGCGGT GCTCAATGAC TTGCTCTACG 
     2221 CAGTGGGAGG CTTTGATGGC AGTACTGGCC TAGCATCGGT GGAAGCCTAC AGCTACAAGA 
     2281 CCAACGAGTG GTTCTTTGTG GCCCCGATGA ACACGCGGCG GAGCAGTGTG GGTGTGGGCG 
     2341 TTGTGGAGGG GAAGCTATAT GCTGTTGGGG GTTATGATGG AGCTTCCCGC CAGTGTCTGA 
     2401 GCACTGTGGA GCAGTACAAC CCAGCGACCA ATGAATGGAT ATACGTGGCG GACATGAGCA 
     2461 CCCGCCGCAG TGGCGCAGGG GTTGGAGTGC TTAGCGGACA GCTGTACGCC ACAGGTGGGC 
     2521 ATGATGGGCC TTTGGTGAGG AAGAGCGTTG AGGTTTACGA TCCTGGAACA AATACCTGGA 
     2581 AGCAAGTGGC AGACATGAAC ATGTGCCGGC GCAACGCAGG GGTCTGTGCA GTAAATGGGC 
     2641 TCCTGTATGT GGTTGGAGGG GATGATGGAT CCTGCAACTT GGCTTCGGTG GAGTACTACA 
     2701 ATCCTGTCAC TGACAAATGG ACGCTGCTTC CAACGAACAT GAGCACGGGG CGGAGCTATG 
     2761 CAGGTGTTGC CGTGATTCAC AAGTCCTTGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     2821 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     2881 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     2941 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3001 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3061 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3121 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3181 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3241 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3301 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3361 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3421 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3481 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3541 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3601 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     3661 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     3721 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     3781 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     3841 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     3901 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     3961 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4021 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4081 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4141 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4201 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4261 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4321 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4381 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4441 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4501 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4561 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4621 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     4681 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     4741 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     4801 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     4861 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     4921 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     4981 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5041 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5101 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5161 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5221 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5281 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5341 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5401 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5461 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5521 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5581 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     5641 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     5701 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     5761 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     5821 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     5881 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     5941 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6001 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6061 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6121 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6181 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6241 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6301 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6361 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6421 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6481 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6541 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6601 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//