LOCUS       RC218368                8190 bp    DNA     circular  SYN 
DEFINITION  GUCY2D (Myc-DDK-tagged)-Human guanylate cyclase 2D, membrane (retina-specific) (GUCY2D)
ACCESSION   NM_000180
VERSION     NM_000180.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4347..4438 
                     /label="Myc-DDK"  
     misc_feature    4468..5053 
                     /label="PolyA signal"  
     misc_feature    4525..4544 
                     /label="Restriction Enzyme"  
     misc_feature    5329..6001 
                     /label="ColE1"  
     misc_feature    6431..7225 
                     /label="Kan/Neo"  
     misc_feature    7260..7562 
                     /label="SV40 ori"  
     misc_feature    7648..8125 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4329..4335 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4329 
                     /label="GUCY2D"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCGCCTGC GCCCGCCGAG CGGGTGGGCT TCCGGACCCC GGGCTCTGCG 
     1081 GTCCCGCGTG GTGGGCTCCG TCCCTGCCCC GCCTCCCCCG GGCCCTGCCC CGGCTCCCGC 
     1141 TCCTGCTGCT CCTGCTTCTG CTGCAGCCCC CCGCCCTCTC CGCCGTGTTC ACGGTGGGGG 
     1201 TCCTGGGCCC CTGGGCTTGC GACCCCATCT TCTCTCGGGC TCGCCCGGAC CTGGCCGCCC 
     1261 GCCTGGCCGC CGCCCGCCTG AACCGCGACC CCGGCCTGGC AGGCGGTCCC CGCTTCGAGG 
     1321 TAGCGCTGCT GCCCGAGCCT TGCCGGACGC CGGGCTCGCT GGGGGCCGTG TCCTCCGCGC 
     1381 TGGCCCGCGT GTCGGGCCTC GTGGGTCCGG TGAACCCTGC GGCCTGCCGG CCAGCCGAGC 
     1441 TGCTCGCCGA AGAAGCCGGG ATCGCGCTGG TGCCCTGGGG CTGCCCCTGG ACGCAGGCGG 
     1501 AGGGCACCAC GGCCCCTGCC GTGACCCCCG CCGCGGATGC CCTCTACGCC CTGCTTCGCG 
     1561 CATTCGGCTG GGCGCGCGTG GCCCTGGTCA CCGCCCCCCA GGACCTGTGG GTGGAGGCGG 
     1621 GACGCTCACT GTCCACGGCA CTCAGGGCCC GGGGGCTGCC TGTCGCCTCC GTGACTTCCA 
     1681 TGGAGCCCTT GGACCTGTCT GGAGCCCGGG AGGCCCTGAG GAAGGTTCGG GACGGGCCCA 
     1741 GGGTCACAGC AGTGATCATG GTGATGCACT CGGTGCTGCT GGGTGGCGAG GAGCAGCGCT 
     1801 ACCTCCTGGA GGCCGCAGAG GAGCTGGGCC TGACCGATGG CTCCCTGGTC TTCCTGCCCT 
     1861 TCGACACGAT CCACTACGCC TTGTCCCCAG GCCCGGAGGC CTTGGCCGCA CTCGCCAACA 
     1921 GCTCCCAGCT TCGCAGGGCC CACGATGCCG TGCTCACCCT CACGCGCCAC TGTCCCTCTG 
     1981 AAGGCAGCGT GCTGGACAGC CTGCGCAGGG CTCAAGAGCG CCGCGAGCTG CCCTCTGACC 
     2041 TCAATCTGCA GCAGGTCTCC CCACTCTTTG GCACCATCTA TGACGCGGTC TTCTTGCTGG 
     2101 CAAGGGGCGT GGCAGAAGCG CGGGCTGCCG CAGGTGGCAG ATGGGTGTCC GGAGCAGCTG 
     2161 TGGCCCGCCA CATCCGGGAT GCGCAGGTCC CTGGCTTCTG CGGGGACCTA GGAGGAGACG 
     2221 AGGAGCCCCC ATTCGTGCTG CTAGACACGG ACGCGGCGGG AGACCGGCTT TTTGCCACAT 
     2281 ACATGCTGGA TCCTGCCCGG GGCTCCTTCC TCTCCGCCGG TACCCGGATG CACTTCCCGC 
     2341 GTGGGGGATC AGCACCCGGA CCTGACCCCT CGTGCTGGTT CGATCCAAAC AACATCTGCG 
     2401 GTGGAGGACT GGAGCCGGGC CTCGTCTTTC TTGGCTTCCT CCTGGTGGTT GGGATGGGGC 
     2461 TGGCTGGGGC CTTCCTGGCC CATTATGTGA GGCACCGGCT ACTTCACATG CAAATGGTCT 
     2521 CCGGCCCCAA CAAGATCATC CTGACCGTGG ACGACATCAC CTTTCTCCAC CCACATGGGG 
     2581 GCACCTCTCG AAAGGTGGCC CAGGGGAGTC GATCAAGTCT GGGTGCCCGC AGCATGTCAG 
     2641 ACATTCGCAG CGGCCCCAGC CAACACTTGG ACAGCCCCAA CATTGGTGTC TATGAGGGAG 
     2701 ACAGGGTTTG GCTGAAGAAA TTCCCAGGGG ATCAGCACAT AGCTATCCGC CCAGCAACCA 
     2761 AGACGGCCTT CTCCAAGCTC CAGGAGCTCC GGCATGAGAA CGTGGCCCTC TACCTGGGGC 
     2821 TTTTCCTGGC TCGGGGAGCA GAAGGCCCTG CGGCCCTCTG GGAGGGCAAC CTGGCTGTGG 
     2881 TCTCAGAGCA CTGCACGCGG GGCTCTCTTC AGGACCTCCT CGCTCAGAGA GAAATAAAGC 
     2941 TGGACTGGAT GTTCAAGTCC TCCCTCCTGC TGGACCTTAT CAAGGGAATA AGGTATCTGC 
     3001 ACCATCGAGG CGTGGCTCAT GGGCGGCTGA AGTCACGGAA CTGCATAGTG GATGGCAGAT 
     3061 TCGTACTCAA GATCACTGAC CACGGCCACG GGAGACTGCT GGAAGCACAG AAGGTGCTAC 
     3121 CGGAGCCTCC CAGAGCGGAG GACCAGCTGT GGACAGCCCC GGAGCTGCTT AGGGACCCAG 
     3181 CCCTGGAGCG CCGGGGAACG CTGGCCGGCG ACGTCTTTAG CTTGGCCATC ATCATGCAAG 
     3241 AAGTAGTGTG CCGCAGTGCC CCTTATGCCA TGCTGGAGCT CACTCCCGAG GAAGTGGTGC 
     3301 AGAGGGTGCG GAGCCCCCCT CCACTGTGTC GGCCCTTGGT GTCCATGGAC CAGGCACCTG 
     3361 TCGAGTGTAT CCTCCTGATG AAGCAGTGCT GGGCAGAGCA GCCGGAACTT CGGCCCTCCA 
     3421 TGGACCACAC CTTCGACCTG TTCAAGAACA TCAACAAGGG CCGGAAGACG AACATCATTG 
     3481 ACTCGATGCT TCGGATGCTG GAGCAGTACT CTAGTAACCT GGAGGATCTG ATCCGGGAGC 
     3541 GCACGGAGGA GCTGGAGCTG GAAAAGCAGA AGACAGACCG GCTGCTTACA CAGATGCTGC 
     3601 CTCCGTCTGT GGCTGAGGCC TTGAAGACGG GGACACCAGT GGAGCCCGAG TACTTTGAGC 
     3661 AAGTGACACT GTACTTTAGT GACATTGTGG GCTTCACCAC CATCTCTGCC ATGAGTGAGC 
     3721 CCATTGAGGT TGTGGACCTG CTCAACGATC TCTACACACT CTTTGATGCC ATCATTGGTT 
     3781 CCCACGATGT CTACAAGGTG GAGACAATAG GGGACGCCTA TATGGTGGCC TCGGGGCTGC 
     3841 CCCAGCGGAA TGGGCAGCGA CACGCGGCAG AGATCGCCAA CATGTCACTG GACATCCTCA 
     3901 GTGCCGTGGG CACTTTCCGC ATGCGCCATA TGCCTGAGGT TCCCGTGCGC ATCCGCATAG 
     3961 GCCTGCACTC GGGTCCATGC GTGGCAGGCG TGGTGGGCCT CACCATGCCG CGGTACTGCC 
     4021 TGTTTGGGGA CACGGTCAAC ACCGCCTCGC GCATGGAGTC CACCGGGCTG CCTTACCGCA 
     4081 TCCACGTGAA CTTGAGCACT GTGGGGATTC TCCGTGCTCT GGACTCGGGC TACCAGGTGG 
     4141 AGCTGCGAGG CCGCACGGAG CTGAAGGGCA AGGGCGCCGA GGACACTTTC TGGCTAGTGG 
     4201 GCAGACGCGG CTTCAACAAG CCCATCCCCA AACCGCCTGA CCTGCAACCG GGGTCCAGCA 
     4261 ACCACGGCAT CAGCCTGCAG GAGATCCCAC CCGAGCGGCG ACGGAAGCTG GAGAAGGCGC 
     4321 GGCCGGGCCA GTTCTCTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4381 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4441 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4501 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4561 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4621 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4681 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4741 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4801 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4861 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4921 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4981 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5041 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5101 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5161 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5221 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5281 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5341 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5401 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5461 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5521 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5581 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5641 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5701 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5761 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5821 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5881 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5941 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6001 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6061 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6121 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6181 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6241 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6301 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6361 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6421 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6481 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6541 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6601 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6661 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6721 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6781 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6841 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6901 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6961 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7021 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7081 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7141 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7201 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7261 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7321 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7381 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7441 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7501 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7561 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7621 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7681 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7741 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7801 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7861 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7921 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7981 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8041 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8101 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8161 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//