LOCUS       RC218273                7146 bp    DNA     circular  SYN 
DEFINITION  MTSS1 (Myc-DDK-tagged)-Human metastasis suppressor 1 (MTSS1)
ACCESSION   NM_014751
VERSION     NM_014751.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3303..3394 
                     /label="Myc-DDK"  
     misc_feature    3424..4009 
                     /label="PolyA signal"  
     misc_feature    3481..3500 
                     /label="Restriction Enzyme"  
     misc_feature    4285..4957 
                     /label="ColE1"  
     misc_feature    5387..6181 
                     /label="Kan/Neo"  
     misc_feature    6216..6518 
                     /label="SV40 ori"  
     misc_feature    6604..7081 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3285..3291 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3285 
                     /label="MTSS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCTGTG ATTGAGAAGG AATGCAGCGC GCTCGGAGGC CTCTTCCAGA 
     1081 CCATCATCAG CGACATGAAG GGGAGCTATC CAGTTTGGGA AGATTTCATA AACAAAGCAG 
     1141 GAAAGCTGCA GTCCCAGCTT CGGACAACAG TAGTAGCAGC AGCTGCCTTC TTGGACGCCT 
     1201 TTCAGAAAGT GGCTGACATG GCCACCAACA CACGTGGTGG GACCAGGGAG ATTGGATCTG 
     1261 CTCTCACCAG GATGTGCATG AGGCACAGAA GCATTGAAGC CAAGCTGAGG CAGTTTTCGA 
     1321 GCGCTTTAAT TGATTGTCTG ATAAACCCAC TTCAAGAACA GATGGAAGAA TGGAAGAAAG 
     1381 TGGCCAACCA GCTGGATAAA GACCACGCAA AAGAATATAA GAAAGCCCGC CAAGAGATAA 
     1441 AAAAGAAGTC CTCGGATACG CTGAAACTGC AGAAGAAAGC AAAAAAAGGG AGAGGTGATA 
     1501 TCCAGCCTCA GTTGGACAGT GCTCTCCAAG ATGTCAATGA TAAGTATCTC TTATTGGAAG 
     1561 AAACAGAAAA GCAGGCTGTC CGGAAGGCTT TGATTGAAGA ACGTGGCCGA TTCTGTACCT 
     1621 TCATCTCTAT GCTGCGGCCA GTGATTGAAG AAGAAATCTC AATGCTAGGG GAAATAACCC 
     1681 ACCTTCAGAC CATCTCGGAA GATCTAAAAA GCCTGACCAT GGACCCTCAC AAACTGCCCT 
     1741 CCTCAAGTGA ACAGGTGATT CTGGACTTGA AAGGTTCTGA TTACAGCTGG TCGTATCAGA 
     1801 CGCCACCCTC TTCCCCCAGC ACCACCATGT CCAGAAAGTC CAGTGTCTGC AGCAGCCTGA 
     1861 ACAGTGTCAA CAGCAGTGAC TCCCGGTCCA GCGGCTCCCA CTCGCATTCC CCCAGCTCAC 
     1921 ATTACCGCTA CCGCAGCTCC AACCTGGCCC AGCAGGCTCC TGTGAGGCTG TCCAGCGTGT 
     1981 CCTCCCATGA CTCAGGATTC ATATCCCAGG ATGCCTTCCA GTCCAAGTCA CCATCCCCCA 
     2041 TGCCGCCAGA GGCCCCCAAC CAGTTGTCTA ACGGGTTTTC TCACTATAGT TTATCAAGTG 
     2101 AGTCCCACGT GGGGCCCACG GGTGCAGGCC TTTTCCCTCA TTGCCTGCCT GCCTCCCGCC 
     2161 TGCTCCCTCG GGTCACCTCT GTCCACCTTC CAGACTACGC TCATTATTAC ACCATTGGGC 
     2221 CCGGCATGTT CCCGTCATCT CAGATCCCTA GCTGGAAGGA CTGGGCTAAG CCTGGGCCCT 
     2281 ATGACCAGCC TCTGGTGAAC ACCCTGCAGC GCCGCAAAGA GAAGCGAGAA CCGGACCCCA 
     2341 ACGGGGGAGG ACCCACTACC GCCAGCGGCC CACCTGCAGC AGCTGAGGAG GCTCAGAGAC 
     2401 CACGGAGCAT GACTGTATCG GCTGCCACCA GGCCTGGTGA GGAGATGGAG GCTTGTGAGG 
     2461 AGCTGGCCCT GGCCCTGTCT CGGGGCCTGC AGCTGGACAC CCAGAGGAGC AGCCGGGACT 
     2521 CGCTTCAGTG CTCCAGCGGC TACAGCACCC AGACAACCAC CCCCTGCTGC TCTGAGGACA 
     2581 CCATCCCTTC CCAAGTTTCA GATTATGATT ATTTCTCTGT AAGTGGTGAC CAGGAGGCAG 
     2641 ATCAGCAGGA GTTCGACAAG TCCTCCACCA TTCCAAGAAA CAGCGACATC AGCCAGTCCT 
     2701 ACCGACGGAT GTTCCAAGCC AAGCGTCCAG CCTCAACTGC TGGCCTCCCC ACCACCCTGG 
     2761 GACCTGCTAT GGTCACTCCA GGGGTTGCAA CTATCCGACG GACCCCTTCC ACCAAGCCTT 
     2821 CTGTCCGCCG GGGAACCATT GGAGCTGGTC CCATCCCCAT CAAGACACCC GTGATCCCTG 
     2881 TCAAGACCCC AACCGTCCCA GACCTCCCAG GGGTGTTGCC AGCCCCTCCA GATGGGCCAG 
     2941 AAGAGCGGGG GGAGCACAGC CCTGAGTCGC CATCTGTGGG TGAGGGCCCC CAAGGTGTCA 
     3001 CCAGCATGCC CTCCTCAATG TGGAGCGGCC AAGCTTCCGT TAACCCTCCA CTTCCAGGCC 
     3061 CGAAGCCCAG TATCCCTGAG GAGCACAGAC AGGCAATTCC AGAAAGTGAA GCTGAAGACC 
     3121 AGGAACGGGA ACCCCCAAGT GCCACTGTCT CCCCAGGCCA GATTCCAGAG AGTGACCCTG 
     3181 CAGACCTGAG CCCAAGGGAT ACTCCACAAG GAGAAGACAT GCTGAACGCC ATCCGAAGGG 
     3241 GCGTGAAACT GAAGAAGACC ACGACAAACG ATCGCTCAGC CCCTCGCTTT TCTACGCGTA 
     3301 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3361 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3421 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3481 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3541 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3601 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3661 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3721 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3781 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3841 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3901 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3961 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4021 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4081 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4141 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4201 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4261 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4321 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4381 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4441 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4501 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4561 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4621 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4681 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4741 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4801 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4861 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4921 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     4981 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5041 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5101 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5161 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5221 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5281 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5341 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5401 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5461 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5521 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5581 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5641 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5701 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5761 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5821 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5881 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     5941 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6001 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6061 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6121 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6181 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6241 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6301 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6361 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6421 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6481 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6541 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6601 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6661 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6721 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6781 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6841 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6901 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     6961 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7021 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7081 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7141 AATTTT
//