LOCUS       RC218157                8028 bp    DNA     circular  SYN 
DEFINITION  DSG1 (Myc-DDK-tagged)-Human desmoglein 1 (DSG1)
ACCESSION   NM_001942
VERSION     NM_001942.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4185..4276 
                     /label="Myc-DDK"  
     misc_feature    4306..4891 
                     /label="PolyA signal"  
     misc_feature    4363..4382 
                     /label="Restriction Enzyme"  
     misc_feature    5167..5839 
                     /label="ColE1"  
     misc_feature    6269..7063 
                     /label="Kan/Neo"  
     misc_feature    7098..7400 
                     /label="SV40 ori"  
     misc_feature    7486..7963 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4167..4173 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4167 
                     /label="DSG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTGGAGT TTCTTCAGAG TAGTTGCAAT GCTGTTCATT TTTCTGGTGG 
     1081 TGGTAGAAGT TAACAGTGAA TTCCGAATCC AGGTAAGAGA TTATAACACT AAAAATGGCA 
     1141 CCATCAAATG GCATTCAATC CGAAGGCAGA AACGTGAATG GATCAAGTTC GCAGCAGCCT 
     1201 GTCGTGAAGG TGAAGACAAC TCAAAGAGGA ACCCAATCGC CAAAATTCAC TCAGATTGTG 
     1261 CTGCAAACCA GCAAGTTACA TACCGCATCT CTGGAGTAGG AATTGATCAG CCACCATATG 
     1321 GGATCTTTGT CATTAATCAG AAAACTGGTG AAATTAATAT AACATCCATA GTTGATCGAG 
     1381 AGGTCACTCC TTTCTTCATT ATCTACTGCC GAGCTCTGAA CTCAATGGGC CAAGATTTAG 
     1441 AGAGGCCTCT AGAGCTCAGA GTCAGGGTTT TGGATATAAA TGACAACCCT CCAGTGTTTT 
     1501 CAATGGCTAC ATTTGCAGGA CAAATAGAAG AAAATTCTAA TGCAAATACA CTGGTGATGA 
     1561 TACTCAATGC TACTGACGCA GATGAACCGA ACAATTTGAA CTCAAAAATA GCCTTCAAGA 
     1621 TTATAAGACA AGAACCTTCA GATTCACCAA TGTTTATTAT CAACAGAAAT ACTGGAGAAA 
     1681 TTCGAACGAT GAATAATTTT CTAGACAGAG AGCAATACGG CCAGTATGCT CTTGCTGTAA 
     1741 GAGGCTCTGA CCGAGATGGT GGGGCAGATG GCATGTCAGC GGAATGTGAG TGCAACATTA 
     1801 AAATCCTCGA TGTCAATGAT AATATCCCTT ACATGGAACA GTCTTCATAT ACCATAGAAA 
     1861 TTCAAGAAAA TACTCTAAAT TCAAATTTGC TCGAGATTAG AGTAATTGAT TTGGATGAAG 
     1921 AGTTCTCAGC TAACTGGATG GCAGTAATTT TCTTTATCTC TGGAAATGAA GGAAATTGGT 
     1981 TTGAGATAGA AATGAATGAA AGAACAAATG TGGGAATTTT AAAGGTTGTT AAGCCCTTAG 
     2041 ATTATGAAGC TATGCAGAGT CTGCAACTCA GTATTGGTGT CAGAAATAAA GCTGAATTTC 
     2101 ATCATTCAAT TATGTCTCAA TATAAACTGA AAGCATCTGC AATTTCTGTG ACTGTGTTAA 
     2161 ATGTAATTGA AGGCCCAGTG TTTCGTCCAG GTTCAAAGAC ATATGTTGTA ACTGGTAATA 
     2221 TGGGATCAAA TGATAAAGTG GGAGACTTTG TAGCTACTGA CCTGGACACA GGTAGACCTT 
     2281 CAACGACTGT TAGGTATGTA ATGGGAAATA ATCCAGCTGA CCTGCTAGCT GTTGATTCAA 
     2341 GAACAGGCAA ACTCACTTTG AAAAATAAAG TTACCAAGGA ACAGTACAAT ATGCTCGGAG 
     2401 GAAAATACCA AGGAACGATT CTCTCTATAG ATGATAATCT TCAAAGAACT TGCACTGGTA 
     2461 CAATTAATAT TAACATTCAA AGTTTTGGTA ATGACGACAG GACTAATACA GAGCCGAACA 
     2521 CTAAAATTAC TACCAATACT GGCAGACAAG AAAGTACTTC TTCCACTAAC TATGATACCA 
     2581 GCACAACTTC TACTGACTCT AGCCAAGTAT ATTCTTCTGA ACCCGGAAAC GGAGCCAAAG 
     2641 ATTTGTTATC AGACAATGTA CATTTTGGTC CTGCTGGCAT TGGACTCCTC ATCATGGGAT 
     2701 TCTTGGTCTT AGGATTGGTC CCATTTTTGA TGATCTGTTG TGATTGTGGA GGTGCTCCTC 
     2761 GTAGTGCAGC TGGCTTTGAG CCTGTTCCCG AATGTTCAGA TGGAGCAATT CATTCATGGG 
     2821 CAGTAGAAGG ACCACAGCCT GAACCCAGGG ATATAACCAC TGTCATACCA CAAATACCAC 
     2881 CTGATAACGC AAATATAATT GAATGCATTG ACAACTCAGG AGTTTATACA AATGAGTATG 
     2941 GTGGCAGAGA AATGCAAGAT CTGGGAGGAG GAGAGAGAAT GACAGGATTT GAACTAACAG 
     3001 AGGGAGTTAA AACTTCAGGA ATGCCTGAGA TATGTCAAGA ATACTCTGGA ACATTAAGAA 
     3061 GAAATTCTAT GAGGGAATGT AGAGAAGGAG GTCTGAATAT GAATTTCATG GAAAGCTACT 
     3121 TCTGTCAGAA AGCATATGCT TACGCAGATG AAGATGAAGG ACGCCCATCT AATGACTGTT 
     3181 TGCTCATATA TGACATCGAA GGTGTAGGTT CCCCTGCTGG CTCTGTGGGT TGTTGTAGCT 
     3241 TCATTGGAGA AGACCTGGAT GACAGCTTCT TGGATACCCT GGGACCTAAA TTTAAGAAGT 
     3301 TGGCAGACAT CAGCCTAGGA AAAGAATCAT ATCCAGACCT TGATCCTTCT TGGCCACCAC 
     3361 AAAGCACTGA ACCAGTTTGC CTTCCTCAGG AAACAGAGCC CGTTGTTAGT GGACACCCAC 
     3421 CAATCTCCCC ACATTTCGGC ACTACCACAG TAATTTCTGA GAGCACCTAT CCCTCGGGAC 
     3481 CTGGTGTACT GCATCCTAAG CCTATTCTCG ATCCTCTGGG CTATGGTAAT GTCACTGTGA 
     3541 CCGAGTCTTA CACCACCTCT GACACTCTGA AGCCCTCTGT GCACGTTCAC GATAACCGAC 
     3601 CAGCATCAAA CGTGGTAGTG ACAGAGAGAG TGGTCGGCCC AATCTCTGGC GCTGATTTGC 
     3661 ATGGAATGTT AGAGATGCCT GACTTGCGAG ATGGGTCGAA TGTTATAGTG ACAGAAAGGG 
     3721 TAATAGCACC AAGCTCTAGT CTACCCACCT CTCTGACTAT CCATCATCCT AGAGAGTCTT 
     3781 CAAATGTGGT AGTGACAGAA AGAGTAATCC AACCAACTTC CGGCATGATA GGTAGTCTGA 
     3841 GTATGCACCC CGAGTTAGCC AATGCCCACA ATGTCATTGT GACAGAGAGG GTTGTTTCTG 
     3901 GTGCTGGCGT AACTGGAATT AGTGGCACCA CTGGGATCAG CGGTGGCATA GGCAGCAGTG 
     3961 GCCTGGTTGG CACCAGCATG GGTGCTGGGA GCGGTGCCCT GAGTGGAGCT GGCATAAGTG 
     4021 GTGGTGGCAT TGGCCTGAGC AGCTTGGGAG GGACAGCCAG CATTGGCCAC ATGAGGAGTT 
     4081 CCTCTGACCA TCACTTTAAC CAAACCATTG GGTCCGCCTC CCCTAGCACA GCTCGAAGTC 
     4141 GAATCACAAA GTATAGTACC GTGCAATATA GCAAGACGCG TACGCGGCCG CTCGAGCAGA 
     4201 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4261 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4321 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4381 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4441 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4501 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4561 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4621 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4681 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4741 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4801 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4861 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4921 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4981 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5041 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5101 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5161 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5221 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5281 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5341 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5401 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5461 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5521 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5581 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5641 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5701 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5761 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5821 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5881 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5941 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6001 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6061 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6121 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6181 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6241 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6301 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6361 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6421 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6481 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6541 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6601 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6661 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6721 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6781 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6841 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6901 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6961 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7021 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7081 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7141 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7201 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7261 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7321 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7381 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7441 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7501 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7561 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7621 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7681 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7741 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7801 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7861 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7921 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7981 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//