LOCUS       RC218131L3                9476 bp    DNA     circular  SYN 
DEFINITION  SPON1 (Myc-DDK-tagged)-Human spondin 1, extracellular matrix protein (SPON1)
ACCESSION   NM_006108
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7767..7947 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8675..9334 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7995..8614 
                     /label="ColE1"  
     misc_feature    5807..5830 
                     /label="DDK"  
     misc_feature    6642..6662 
                     /label="LR50"  
     misc_feature    5759..5830 
                     /label="MYC"  
     misc_feature    5840..5905 
                     /label="P2A"  
     misc_feature    5906..6505 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6651..7242 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5741..5747 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5741 
                     /label="SPON1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGGCTGTCC CCGGCGCCCC TGAAGCTGAG 
     3361 CCGGACTCCG GCACTGCTGG CCCTGGCGCT GCCCCTGGCC GCGGCGCTGG CCTTCTCCGA 
     3421 CGAGACCCTG GACAAAGTGC CCAAGTCAGA GGGCTACTGC AGCCGTATCC TGCGCGCCCA 
     3481 GGGCACGCGG CGCGAGGGCT ACACCGAGTT CAGCCTCCGC GTGGAGGGCG ACCCCGACTT 
     3541 CTACAAGCCG GGAACCAGCT ACCGCGTAAC ACTTTCAGCT GCTCCTCCCT CCTACTTCAG 
     3601 AGGATTCACA TTAATTGCCC TCAGAGAGAA CAGAGAGGGT GATAAGGAAG AAGACCATGC 
     3661 TGGGACCTTC CAGATCATAG ACGAAGAAGA AACTCAGTTT ATGAGCAATT GCCCTGTTGC 
     3721 AGTCACTGAA AGCACTCCAC GGAGGAGGAC CCGGATCCAG GTGTTTTGGA TAGCACCACC 
     3781 AGCGGGAACA GGCTGCGTGA TTCTGAAGGC CAGCATCGTA CAAAAACGCA TTATTTATTT 
     3841 TCAAGATGAG GGCTCTCTGA CCAAGAAACT TTGTGAACAA GATTCCACAT TTGATGGGGT 
     3901 GACTGACAAA CCCATCTTAG ACTGCTGTGC CTGCGGAACT GCCAAGTACA GACTCACATT 
     3961 TTATGGGAAT TGGTCCGAGA AGACACACCC AAAGGATTAC CCTCGTCGGG CCAACCACTG 
     4021 GTCTGCGATC ATCGGAGGAT CCCACTCCAA GAATTATGTA CTGTGGGAAT ATGGAGGATA 
     4081 TGCCAGCGAA GGCGTCAAAC AAGTTGCAGA ATTGGGCTCA CCCGTGAAAA TGGAGGAAGA 
     4141 AATTCGACAA CAGAGTGATG AGGTCCTCAC CGTCATCAAA GCCAAAGCCC AGTGGCCAGC 
     4201 CTGGCAGCCT CTCAACGTGA GAGCAGCACC TTCAGCTGAA TTTTCCGTGG ACAGAACGCG 
     4261 CCATTTAATG TCCTTCCTGA CCATGATGGG CCCTAGTCCC GACTGGAACG TAGGCTTATC 
     4321 TGCAGAAGAT CTGTGCACCA AGGAATGTGG CTGGGTCCAG AAGGTGGTGC AAGACCTGAT 
     4381 TCCCTGGGAC GCTGGCACCG ACAGCGGGGT GACCTATGAG TCACCCAACA AACCCACCAT 
     4441 TCCCCAGGAG AAAATCCGGC CCCTGACCAG CCTGGACCAT CCTCAGAGTC CTTTCTATGA 
     4501 CCCAGAGGGT GGGTCCATCA CTCAAGTAGC CAGAGTTGTC ATCGAGAGAA TCGCACGGAA 
     4561 GGGTGAACAA TGCAATATTG TACCTGACAA TGTCGATGAT ATTGTAGCTG ACCTGGCTCC 
     4621 AGAAGAGAAA GATGAAGATG ACACCCCTGA AACCTGCATC TACTCCAACT GGTCCCCATG 
     4681 GTCCGCCTGC AGCTCCTCCA CCTGTGACAA AGGCAAGAGG ATGCGACAGC GCATGCTGAA 
     4741 AGCACAGCTG GACCTCAGCG TCCCCTGCCC TGACACCCAG GACTTCCAGC CCTGCATGGG 
     4801 CCCTGGCTGC AGTGACGAAG ACGGCCCCAC CTGCACCATG TCCGAGTGGA TCACCTGGTC 
     4861 GCCCTGCAGC ATCTCCTGCG GCATGGGCAT GAGGTCCCGG GAGAGGTATG TGAAGCAGTT 
     4921 CCCGGAGGAC GGCTCCGTGT GCACGCTGCC CACTGAGGAA ACGGAGAAGT GCACGGTCAA 
     4981 CGAGGAGTGC TCTCCCAGCA GCTGCCTGAT GACCGAGTGG GGCGAGTGGG ACGAGTACAG 
     5041 CGCCACCTGC GGCATGGGCA TGAAGAAGCG GCACCGCATG ATCAAGATGA ACCCCGCAGA 
     5101 TGGCTCCATG TGCAAAGCCG AGACATCACA GGCAGAGAAG CGCATGATGC CAGAGTGCCA 
     5161 CACCATCCCA TGCTTGCTGT CCCCATGGTC CGAGTGGAGT GACTGCAGCG TGACCTGCGG 
     5221 GAAGGGCATG CGAACCCGAC AGCGGATGCT CAAGTCTCTG GCAGAACTTG GAGACTGCAA 
     5281 TGAGGATCTG GAGCAGGTGG AGAAGTGCAT GCTCCCTGAA TGCCCCATTG ACTGTGAGCT 
     5341 CACCGAGTGG TCCCAGTGGT CGGAATGTAA CAAGTCATGT GGGAAAGGCC ACGTGATTCG 
     5401 AACCCGGATG ATCCAAATGG AGCCTCAGTT TGGAGGTGCA CCCTGCCCAG AGACTGTGCA 
     5461 GCGAAAAAAG TGCCGCATCC GAAAATGCCT TCGAAATCCA TCCATCCAAA AGCTACGCTG 
     5521 GAGGGAGGCC CGAGAGAGCC GGCGGAGTGA GCAGCTGAAG GAAGAGTCTG AAGGGGAGCA 
     5581 GTTCCCAGGT TGTAGGATGC GCCCATGGAC GGCCTGGTCA GAATGCACCA AACTGTGCGG 
     5641 AGGTGGAATT CAGGAACGTT ACATGACTGT AAAGAAGAGA TTCAAAAGCT CCCAGTTTAC 
     5701 CAGCTGCAAA GACAAGAAGG AGATCAGAGC ATGCAATGTT CATCCTTGTA CGCGTACGCG 
     5761 GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA 
     5821 CAAGGATGAC GACGATAAGG TTTGGGTAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA 
     5881 GCAGGCTGGA GACGTGGAGG AGAACCCTGG ACCTATGACC GAGTACAAGC CCACGGTGCG 
     5941 CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA 
     6001 CTACCCCGCC ACGCGCCACA CCGTCGATCC GGATCGCCAC ATCGAGCGGG TCACCGAGCT 
     6061 GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA 
     6121 CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC 
     6181 CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT 
     6241 GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG 
     6301 CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA 
     6361 GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC 
     6421 CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG 
     6481 CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGATGTACA CCCAAACGGC CGGCCGCGGT 
     6541 CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG 
     6601 TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT CGATATCAAG CTTATCGATA 
     6661 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 
     6721 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 
     6781 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 
     6841 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 
     6901 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 
     6961 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 
     7021 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG 
     7081 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 
     7141 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 
     7201 GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC 
     7261 GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC 
     7321 AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA 
     7381 CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA 
     7441 AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT 
     7501 CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC AGAACTACAC ACCAGGGCCA 
     7561 GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCAAGAG 
     7621 AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT TACACCCTGT GAGCCTGCAT 
     7681 GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT 
     7741 CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT CTCTCTGGTT AGACCAGATC 
     7801 TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG 
     7861 CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC 
     7921 CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA 
     7981 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT 
     8041 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 
     8101 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 
     8161 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA 
     8221 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 
     8281 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 
     8341 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 
     8401 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA 
     8461 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC 
     8521 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 
     8581 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 
     8641 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGATTACGCC CCGCCCTGCC 
     8701 ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA 
     8761 CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT 
     8821 TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC 
     8881 TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG 
     8941 GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT 
     9001 GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA 
     9061 AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA 
     9121 TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA 
     9181 ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT 
     9241 GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT 
     9301 GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATACTCTTC CTTTTTCAAT 
     9361 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     9421 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
//