LOCUS RC218131 7302 bp DNA circular SYN DEFINITION SPON1 (Myc-DDK-tagged)-Human spondin 1, extracellular matrix protein (SPON1) ACCESSION NM_006108 VERSION NM_006108.1 SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3459..3550 /label="Myc-DDK" misc_feature 3580..4165 /label="PolyA signal" misc_feature 3637..3656 /label="Restriction Enzyme" misc_feature 4441..5113 /label="ColE1" misc_feature 5543..6337 /label="Kan/Neo" misc_feature 6372..6674 /label="SV40 ori" misc_feature 6760..7237 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3441..3447 /label="Restriction Enzyme" misc_feature 1020..3441 /label="SPON1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GAGGCTGTCC CCGGCGCCCC TGAAGCTGAG CCGGACTCCG GCACTGCTGG 1081 CCCTGGCGCT GCCCCTGGCC GCGGCGCTGG CCTTCTCCGA CGAGACCCTG GACAAAGTGC 1141 CCAAGTCAGA GGGCTACTGC AGCCGTATCC TGCGCGCCCA GGGCACGCGG CGCGAGGGCT 1201 ACACCGAGTT CAGCCTCCGC GTGGAGGGCG ACCCCGACTT CTACAAGCCG GGAACCAGCT 1261 ACCGCGTAAC ACTTTCAGCT GCTCCTCCCT CCTACTTCAG AGGATTCACA TTAATTGCCC 1321 TCAGAGAGAA CAGAGAGGGT GATAAGGAAG AAGACCATGC TGGGACCTTC CAGATCATAG 1381 ACGAAGAAGA AACTCAGTTT ATGAGCAATT GCCCTGTTGC AGTCACTGAA AGCACTCCAC 1441 GGAGGAGGAC CCGGATCCAG GTGTTTTGGA TAGCACCACC AGCGGGAACA GGCTGCGTGA 1501 TTCTGAAGGC CAGCATCGTA CAAAAACGCA TTATTTATTT TCAAGATGAG GGCTCTCTGA 1561 CCAAGAAACT TTGTGAACAA GATTCCACAT TTGATGGGGT GACTGACAAA CCCATCTTAG 1621 ACTGCTGTGC CTGCGGAACT GCCAAGTACA GACTCACATT TTATGGGAAT TGGTCCGAGA 1681 AGACACACCC AAAGGATTAC CCTCGTCGGG CCAACCACTG GTCTGCGATC ATCGGAGGAT 1741 CCCACTCCAA GAATTATGTA CTGTGGGAAT ATGGAGGATA TGCCAGCGAA GGCGTCAAAC 1801 AAGTTGCAGA ATTGGGCTCA CCCGTGAAAA TGGAGGAAGA AATTCGACAA CAGAGTGATG 1861 AGGTCCTCAC CGTCATCAAA GCCAAAGCCC AGTGGCCAGC CTGGCAGCCT CTCAACGTGA 1921 GAGCAGCACC TTCAGCTGAA TTTTCCGTGG ACAGAACGCG CCATTTAATG TCCTTCCTGA 1981 CCATGATGGG CCCTAGTCCC GACTGGAACG TAGGCTTATC TGCAGAAGAT CTGTGCACCA 2041 AGGAATGTGG CTGGGTCCAG AAGGTGGTGC AAGACCTGAT TCCCTGGGAC GCTGGCACCG 2101 ACAGCGGGGT GACCTATGAG TCACCCAACA AACCCACCAT TCCCCAGGAG AAAATCCGGC 2161 CCCTGACCAG CCTGGACCAT CCTCAGAGTC CTTTCTATGA CCCAGAGGGT GGGTCCATCA 2221 CTCAAGTAGC CAGAGTTGTC ATCGAGAGAA TCGCACGGAA GGGTGAACAA TGCAATATTG 2281 TACCTGACAA TGTCGATGAT ATTGTAGCTG ACCTGGCTCC AGAAGAGAAA GATGAAGATG 2341 ACACCCCTGA AACCTGCATC TACTCCAACT GGTCCCCATG GTCCGCCTGC AGCTCCTCCA 2401 CCTGTGACAA AGGCAAGAGG ATGCGACAGC GCATGCTGAA AGCACAGCTG GACCTCAGCG 2461 TCCCCTGCCC TGACACCCAG GACTTCCAGC CCTGCATGGG CCCTGGCTGC AGTGACGAAG 2521 ACGGCTCCAC CTGCACCATG TCCGAGTGGA TCACCTGGTC GCCCTGCAGC ATCTCCTGCG 2581 GCATGGGCAT GAGGTCCCGG GAGAGGTATG TGAAGCAGTT CCCGGAGGAC GGCTCCGTGT 2641 GCACGCTGCC CACTGAGGAA ACGGAGAAGT GCACGGTCAA CGAGGAGTGC TCTCCCAGCA 2701 GCTGCCTGAT GACCGAGTGG GGCGAGTGGG ACGAGTACAG CGCCACCTGC GGCATGGGCA 2761 TGAAGAAGCG GCACCGCATG ATCAAGATGA ACCCCGCAGA TGGCTCCATG TGCAAAGCCG 2821 AGACATCACA GGCAGAGAAG TGCATGATGC CAGAGTGCCA CACCATCCCA TGCTTGCTGT 2881 CCCCATGGTC CGAGTGGAGT GACTGCAGCG TGACCTGCGG GAAGGGCATG CGAACCCGAC 2941 AGCGGATGCT CAAGTCTCTG GCAGAACTTG GAGACTGCAA TGAGGATCTG GAGCAGGTGG 3001 AGAAGTGCAT GCTCCCTGAA TGCCCCATTG ACTGTGAGCT CACCGAGTGG TCCCAGTGGT 3061 CGGAATGTAA CAAGTCATGT GGGAAAGGCC ACGTGATTCG AACCCGGATG ATCCAAATGG 3121 AGCCTCAGTT TGGAGGTGCA CCCTGCCCAG AGACTGTGCA GCGAAAAAAG TGCCGCATCC 3181 GAAAATGCCT TCGAAATCCA TCCATCCAAA AGCTACGCTG GAGGGAGGCC CGAGAGAGCC 3241 GGCGGAGTGA GCAGCTGAAG GAAGAGTCTG AAGGGGAGCA GTTCCCAGGT TGTAGGATGC 3301 GCCCATGGAC GGCCTGGTCA GAATGCACCA AACTGTGCGG AGGTGGAATT CAGGAACGTT 3361 ACATGACTGT AAAGAAGAGA TTCAAAAGCT CCCAGTTTAC CAGCTGCAAA GACAAGAAGG 3421 AGATCAGAGC ATGCAATGTT CATCCTTGTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 3481 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 3541 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 3601 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 3661 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 3721 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 3781 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 3841 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 3901 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 3961 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 4021 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 4081 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 4141 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 4201 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 4261 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 4321 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 4381 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 4441 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 4501 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 4561 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 4621 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 4681 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 4741 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 4801 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 4861 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 4921 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 4981 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 5041 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 5101 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 5161 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 5221 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 5281 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 5341 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 5401 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 5461 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 5521 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 5581 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 5641 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 5701 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 5761 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 5821 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 5881 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 5941 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 6001 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 6061 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 6121 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 6181 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 6241 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 6301 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 6361 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 6421 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 6481 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 6541 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 6601 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 6661 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 6721 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 6781 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 6841 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 6901 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 6961 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 7021 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 7081 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 7141 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 7201 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 7261 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT //