LOCUS       RC218128                7803 bp    DNA     circular  SYN 
DEFINITION  WDR59 (Myc-DDK-tagged)-Human WD repeat domain 59 (WDR59)
ACCESSION   NM_030581
VERSION     NM_030581.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3960..4051 
                     /label="Myc-DDK"  
     misc_feature    4081..4666 
                     /label="PolyA signal"  
     misc_feature    4138..4157 
                     /label="Restriction Enzyme"  
     misc_feature    4942..5614 
                     /label="ColE1"  
     misc_feature    6044..6838 
                     /label="Kan/Neo"  
     misc_feature    6873..7175 
                     /label="SV40 ori"  
     misc_feature    7261..7738 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3942..3948 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3942 
                     /label="WDR59"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGCGA TGGAGCAGCG AAAACGTGGT TGTAGAGTTC CGTGACTCCC 
     1081 AGGCAACTGC GATGTCTGTG GACTGTCTTG GGCAGCATGC AGTGCTTTCT GGCCGCAGAT 
     1141 TCTTATACAT CGTCAATCTA GATGCCCCTT TCGAAGGTCA CCGAAAGATC TCTCGCCAGA 
     1201 GCAAATGGGA CATTGGAGCT GTGCAGTGGA ATCCTCATGA CAGCTTTGCA CACTATTTTG 
     1261 CGGCTTCGAG TAACCAACGA GTAGACCTTT ACAAGTGGAA AGACGGCAGT GGGGAAGTTG 
     1321 GCACAACCTT ACAAGGCCAC ACTCGTGTCA TCAGCGACTT GGACTGGGCG GTGTTTGAGC 
     1381 CTGACCTCCT GGTTACCAGC TCTGTGGACA CCTACATCTA CATTTGGGAT ATCAAAGACA 
     1441 CAAGGAAACC TACTGTTGCA CTGTCTGCTG TTGCGGGTGC CTCCCAGGTC AAATGGAATA 
     1501 AAAAAAATGC TAACTGCCTT GCCACCAGCC ATGACGGCGA TGTGCGGATA TGGGATAAGA 
     1561 GGAAACCCAG TACAGCAGTG GAATATCTAG CCGCCCACCT CTCCAAAATC CATGGCCTGG 
     1621 ACTGGCACCC AGACAGCGAG CACATTCTTG CTACCTCCAG TCAAGACAAT TCTGTGAAGT 
     1681 TCTGGGATTA CCGCCAGCCT CGGAAATACC TCAATATTCT TCCTTGCCAG GTGCCTGTCT 
     1741 GGAAGGCCAG ATACACACCT TTCAGCAATG GATTGGTGAC TGTGATGGTT CCCCAGCTGC 
     1801 GGAGGGAAAA CAGCCTTCTC CTGTGGAATG TCTTTGACTT GAACACCCCA GTCCACACCT 
     1861 TCGTGGGGCA TGATGATGTG GTCCTGGAGT TCCAGTGGAG GAAGCAGAAG GAAGGGTCCA 
     1921 AGGACTATCA ACTGGTGACG TGGTCCCGGG ATCAGACCTT GAGAATGTGG CGGGTGGATT 
     1981 CCCAGATGCA GAGGCTTTGT GCAAATGACA TATTAGATGG TGTTGATGAG TTCATTGAGA 
     2041 GTATTTCCCT TCTGCCGGAA CCTGAGAAGA CCCTGCACAC TGAAGATACA GATCACCAGC 
     2101 ACACTGCAAG CCATGGGGAG GAAGAAGCCC TAAAAGAAGA TCCCCCTAGA AATCTCCTGG 
     2161 AAGAGAGGAA ATCAGATCAA CTGGGGCTGC CTCAGACCTT GCAGCAGGAA TTCTCCCTGA 
     2221 TCAATGTGCA AATCCGGAAT GTCAATGTGG AGATGGATGC GGCAGACAGG AGCTGCACAG 
     2281 TGTCTGTGCA CTGCAGCAAC CATCGTGTCA AGATGCTGGT GAAGTTCCCT GCACAGTACC 
     2341 CAAACAACGC CGCCCCTTCC TTCCAGTTTA TTAACCCCAC AACCATCACA TCCACCATGA 
     2401 AAGCTAAGCT GCTGAAGATC CTGAAGGACA CAGCCCTGCA GAAAGTGAAG CGTGGCCAGA 
     2461 GCTGCCTGGA GCCCTGCCTG CGCCAGCTCG TCTCCTGCCT TGAGTCCTTT GTGAACCAGG 
     2521 AAGACAGCGC TTCCAGCAAC CCGTTTGCAC TCCCCAACTC TGTCACTCCC CCCTTACCGA 
     2581 CGTTTGCGCG GGTGACCACG GCTTACGGGT CGTACCAGGA CGCCAACATT CCCTTTCCTA 
     2641 GGACTTCTGG GGCCAGGTTC TGCGGAGCAG GTTACCTGGT ATATTTCACA AGGCCCATGA 
     2701 CAATGCATCG GGCGGTGTCT CCCACAGAGC CTACTCCGAG ATCTCTCTCA GCCTTGTCTG 
     2761 CTTATCACAC TGGCTTGATC GCGCCCATGA AGATCCGCAC AGAGGCCCCT GGGAACCTTC 
     2821 GTTTATACAG TGGGAGCCCC ACTCGCAGCG AGAAAGAGCA GGTCTCCATC AGCTCCTTCT 
     2881 ACTACAAGGA GCGGAAATCA AGACGATGGA AAAGTAAGCG TGAGGGATCA GACTCTGGCA 
     2941 ATCGACAGAT CAAGGCTGCT GGGAAAGTCA TCATCCAGGA TATTGCTTGC CTCCTGCCTG 
     3001 TTCACAAATC GCTGGGAGAG CTGTACATAT TGAATGTGAA TGATATTCAG GAAACATGTC 
     3061 AGAAGAATGC CGCCTCTGCC TTGCTCGTTG GAAGAAAGGA TCTTGTCCAG GTTTGGTCGC 
     3121 TGGCTACGGT AGCTACAGAT CTTTGCCTTG GTCCGAAATC TGACCCAGAT TTGGAAACAC 
     3181 CCTGGGCTCG ACATCCATTT GGGCGGCAGC TGCTGGAGTC CCTGTTGGCT CACTATTGCC 
     3241 GGCTCCGGGA TGTTCAGACA CTGGCGATGC TCTGTAGCGT GTTTGAAGCC CAGTCTCGGC 
     3301 CTCAGGGGCT ACCAAACCCC TTTGGGCCTT TTCCTAACCG TTCTTCTAAT CTTGTGGTGT 
     3361 CCCATAGTCG ATATCCTAGC TTTACCTCTT CTGGTTCCTG CTCCAGTATG TCAGACCCAG 
     3421 GGCTCAACAC TGGCGGCTGG AACATAGCGG GAAGAGAGGC AGAGCACTTG TCCTCCCCTT 
     3481 GGGGAGAATC CTCACCAGAA GAGCTCCGCT TTGGGAGTCT GACCTACAGT GATCCCCGTG 
     3541 AGCGAGAACG CGACCAGCAT GATAAAAATA AAAGGCTCCT GGACCCCGCC AATACCCAGC 
     3601 AATTTGATGA CTTTAAGAAA TGCTATGGGG AAATCCTCTA CCGTTGGGGT CTGAGAGAGA 
     3661 AGCGAGCTGA AGTGTTGAAG TTTGTCTCCT GTCCTCCTGA CCCTCACAAA GGGATCGAGT 
     3721 TCGGCGTGTA CTGCAGCCAC TGCCGGAGTG AGGTCCGTGG CACGCAGTGT GCCATCTGCA 
     3781 AAGGCTTCAC GTTCCAGTGT GCCATCTGTC ACGTGGCTGT GCGGGGATCG TCCAATTTCT 
     3841 GCCTGACCTG TGGGCACGGT GGCCACACCA GCCACATGAT GGAGTGGTTT CGGACCCAGG 
     3901 AGGTGTGTCC CACCGGGTGT GGGTGCCACT GCCTGCTTGA AAGCACTTTC ACGCGTACGC 
     3961 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4021 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4081 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4141 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4201 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4261 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4321 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4381 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4441 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4501 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4561 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4621 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4681 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4741 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4801 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4861 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4921 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4981 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5041 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5101 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5161 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5221 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5281 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5341 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5401 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5461 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5521 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5581 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5641 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5701 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5761 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5821 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5881 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5941 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6001 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6061 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6121 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6181 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6241 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6301 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6361 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6421 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6481 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6541 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6601 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6661 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6721 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6781 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6841 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6901 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6961 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7021 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7081 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7141 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7201 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7261 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7321 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7381 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7441 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7501 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7561 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7621 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7681 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7741 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7801 TTT
//