LOCUS       RC218059                7530 bp    DNA     circular  SYN 
DEFINITION  LRRC66 (Myc-DDK-tagged)-Human leucine rich repeat containing 66 (LRRC66)
ACCESSION   NM_001024611
VERSION     NM_001024611.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3685..3776 
                     /label="Myc-DDK"  
     misc_feature    3806..4391 
                     /label="PolyA signal"  
     misc_feature    3863..3882 
                     /label="Restriction Enzyme"  
     misc_feature    4667..5339 
                     /label="ColE1"  
     misc_feature    5769..6563 
                     /label="Kan/Neo"  
     misc_feature    6598..6900 
                     /label="SV40 ori"  
     misc_feature    6986..7463 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3660..3667 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3660 
                     /label="LRRC66"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAAACCTC TATTTCAGAG TCATTACCAT AGTTATAGGT CTTTATTTTA 
     1081 CTGGAATAAT GACAAATGCA TCAAGAAAAA GCAATATTTT ATTCAATTCT GAATGCCAAT 
     1141 GGAATGAATA TATTCTGACA AATTGTTCTT TTACCGGAAA GTGTGATATA CCTGTGGACA 
     1201 TATCACAGAC AGCAGCCACT GTGGATGTAA GTTTCAATTT CTTTAGAGTT CTCTTACAGT 
     1261 CTCACACGAA AAAAGAAGAG TGGAAAATAA AACATCTGGA CCTCAGTAAC AATCTCATAT 
     1321 CAAAAATAAC CTTAAGCCCT TTTGCATATT TACATGCTTT GGAAGTGTTA AACCTCAGCA 
     1381 ACAATGCCAT CCACTCCCTC TCATTGGATC TACTCAGTCC TAAGTCCTCA TGGGTGAAAC 
     1441 GCCACAGAAG CAGCTTCAGA AACAGGTTTC CATTGCTGAA GGTGCTCATT CTTCAAAGAA 
     1501 ATAAACTCAG TGACACTCCC AAGGGACTGT GGAAACTGAA GTCATTGCAG AGTTTGGATC 
     1561 TGTCATTCAA TGGGATATTG CAAATAGGGT GGTCTGATTT TCACAACTGC CTGCAACTGG 
     1621 AGAATCTCTG TTTAAAGAGC AACAAGATAT TCAAAATTCC CCCACAAGCC TTCAAGGACC 
     1681 TCAAAAAATT ACAGGTCATA GACCTTAGCA ACAATGCTCT GATTACCATC CTACCAATGA 
     1741 TGATCATAGC TCTAGAATTT CCCCATCTAG TGGTTGACTT GGCTGATAAT AACTGGCAGT 
     1801 GTGATGATAG TGTGGCAGTC TTTCAAAATT TTATTTCTGA ATCCTGGAGG AAAAAGTGGA 
     1861 ATGTCATTTG CAACAGGTCT ATAGGGAGTG AGGAGGCCAA CGGGGGCACT CCCCAGAGCA 
     1921 GGATTTCCAG GGAAACCCGC CTTCCTCCCA TTCATCTGCA TCGCATGAAA AGCCTCATAA 
     1981 GGAGCAAAGC AGAGAGGCCC CAGGGAGGAA GGCACACGGG CATTTCTACT CTGGGGAAGA 
     2041 AGGCAAAGGC CGGCTCTGGT CTCAGGAAGA AGCAGAGACG GCTGCCAAGG AGTGTTAGAA 
     2101 GCACCCGCGA TGTGCAGGCT GCCGGCAAAA AAGAGGACGC TCCCCAGGAC CTGGCTCTGG 
     2161 CGGTGTGCCT GTCAGTGTTC ATCACATTCC TTGTCGCCTT CAGCCTGGGG GCTTTCACAA 
     2221 GGCCTTATGT TGACAGACTG TGGCAAAAAA AGTGCCAGAG CAAAAGCCCT GGCCTGGACA 
     2281 ACGCGTATTC AAACGAGGGC TTCTACGATG ACATGGAAGC TGCGGGGCAC ACACCACACC 
     2341 CAGAGACCCA TCTGCGCCAA GTATTTCCTC ATCTAAGCCT CTACGAGAAC CAGACCCCTT 
     2401 TCTGGGTGAC ACAGCCACAC CCACACGCCA CCGTAATTCC TGATAGAACT CTGGGAAGGA 
     2461 GCAGAAAGGA TCCTGGCAGT TCGCAGAGCC CAGGACAGTG CGGGGACAAC ACCGGGGCAG 
     2521 GAAGTGGAAA TGATGGTGCA GTCTATTCCA TTCTCCAGAG ACATCCACAT GCCGGTAACC 
     2581 GTGAACTAAT GTCAGCAGCG CAGGACCACA TCCATAGGAA TGATATTCTC GGAGAATGGA 
     2641 CTTATGAAAC TGTGGCCCAG GAAGAGCCTC TCAGTGCACA TTCAGTGGGC GTCTCTTCTG 
     2701 TAGCTGGCAC GTCTCACGCT GTCTCTGGCT CAAGCCGTTA TGATTCCAAT GAATTAGACC 
     2761 CTTCCCTCTC CGGAGAAATA ACAGCTTCCC TCTGTAAAAT GCTAACACAT GCAGAAGCAC 
     2821 AGAGGACTGG AGATAGTAAG GAAAGAGGGG GCACTGAACA GTCACTTTGG GACTCGCAGA 
     2881 TGGAATTTTC TAAGGAAAGG CAAGTGAGTT CATCCATTGA TTTGCTGAGC ATACAGCAGC 
     2941 CAAGGCTGTC CGGGGCAAGG GCTGAGGAAG CGCTTTCAGC CCACTACAGC GAGGTTCCAT 
     3001 ACGGTGACCC AAGAGACACA GGCCCATCAG TCTTTCCTCC AAGATGGGAC AGTGGCCTGG 
     3061 ATGTCACTCC TGCTAACAAG GAACCAGTGC AGAAATCCAC TCCTTCTGAC ACTTGCTGTG 
     3121 AGTTGGAGAG TGACTGTGAC TCTGATGAGG GGTCTCTGTT CACTCTGAGC TCCATAAGTT 
     3181 CAGAGAGTGC AAGGAGCAAG ACTGAAGAGG CAGTGCCTGA TGAGGAGTCC CTGCAGGACG 
     3241 AGAGCTCAGG GGCAAGCAAG GACAATGTGA CGGCTGTAGA CAGTCTTGAG GAAAATGTTA 
     3301 CCTTCCAAAC AATTCCAGGG AAATGCAAGA ATCAAGAAGA TCCCTTTGAA AAACCTCTCA 
     3361 TTTCTGCTCC AGACTCTGGC ATGTACAAGA CTCATCTGGA AAATGCCTCT GACACTGATA 
     3421 GATCTGAGGG CCTGTCACCC TGGCCCAGGT CACCAGGGAA TAGTCCCTTA GGGGATGAGT 
     3481 TTCCGGGCAT GTTCACTTAT GATTATGACA CAGCTCTTCA ATCCAAGGCA GCAGAATGGC 
     3541 ATTGCTCACT TAGAGACTTA GAATTTTCAA ATGTGGACGT TTTACAGCAA ACACCACCAT 
     3601 GTTCTGCTGA AGTTCCCTCA GATCCTGATA AGGCTGCCTT CCATGAAAGA GACTCAGACA 
     3661 TTTTAAAAAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3721 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3781 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3841 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3901 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3961 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4021 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4081 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4141 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4201 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4261 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4321 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4381 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4441 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4501 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4561 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4621 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4681 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4741 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4801 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4861 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4921 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4981 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5041 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5101 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5161 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5221 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5281 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5341 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5401 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5461 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5521 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5581 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5641 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5701 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5761 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5821 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5881 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5941 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6001 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6061 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6121 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6181 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6241 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6301 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6361 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6421 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6481 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6541 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6601 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6661 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6721 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6781 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6841 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6901 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6961 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7021 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7081 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7141 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7201 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7261 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7321 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7381 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7441 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7501 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//