LOCUS       RC218041                9342 bp    DNA     circular  SYN 
DEFINITION  COL2A1 (Myc-DDK-tagged)-Human collagen, type II, alpha 1 (COL2A1), transcript variant 1
ACCESSION   NM_001844
VERSION     NM_001844.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5499..5590 
                     /label="Myc-DDK"  
     misc_feature    5620..6205 
                     /label="PolyA signal"  
     misc_feature    5677..5696 
                     /label="Restriction Enzyme"  
     misc_feature    6481..7153 
                     /label="ColE1"  
     misc_feature    7583..8377 
                     /label="Kan/Neo"  
     misc_feature    8412..8714 
                     /label="SV40 ori"  
     misc_feature    8800..9277 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5481..5487 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5481 
                     /label="COL2A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTCGCCTC GGGGCTCCCC AGTCGCTGGT GCTGCTGACG CTGCTCGTCG 
     1081 CCGCTGTCCT TCGGTGTCAG GGCCAGGATG TCCAGGAGGC TGGCAGCTGT GTGCAGGATG 
     1141 GGCAGAGGTA TAATGATAAG GATGTGTGGA AGCCGGAGCC CTGCCGGATC TGTGTCTGTG 
     1201 ACACTGGGAC TGTCCTCTGC GACGACATAA TCTGTGAAGA CGTGAAAGAC TGCCTCAGCC 
     1261 CTGAGATCCC CTTCGGAGAG TGCTGCCCCA TCTGCCCAAC TGACCTCGCC ACTGCCAGTG 
     1321 GGCAACCAGG ACCAAAGGGA CAGAAAGGAG AACCTGGAGA CATCAAGGAT ATTGTAGGAC 
     1381 CCAAAGGACC TCCTGGGCCT CAGGGACCTG CAGGGGAACA AGGACCCAGA GGGGATCGTG 
     1441 GTGACAAAGG TGAAAAAGGT GCCCCTGGAC CTCGTGGCAG AGATGGAGAA CCTGGGACCC 
     1501 CTGGAAATCC TGGCCCCCCT GGTCCTCCCG GCCCCCCTGG TCCCCCTGGT CTTGGTGGAA 
     1561 ACTTTGCTGC CCAGATGGCT GGAGGATTTG ATGAAAAGGC TGGTGGCGCC CAGTTGGGAG 
     1621 TAATGCAAGG ACCAATGGGC CCCATGGGAC CTCGAGGACC TCCAGGCCCT GCAGGTGCTC 
     1681 CTGGGCCTCA AGGATTTCAA GGCAATCCTG GTGAACCTGG TGAACCTGGT GTCTCTGGTC 
     1741 CCATGGGTCC CCGTGGTCCT CCTGGTCCCC CTGGAAAGCC TGGTGATGAT GGTGAAGCTG 
     1801 GAAAACCTGG AAAAGCTGGT GAAAGGGGTC CGCCTGGTCC TCAGGGTGCT CGTGGTTTCC 
     1861 CAGGAACCCC AGGCCTTCCT GGTGTCAAAG GTCACAGAGG TTATCCAGGC CTGGACGGTG 
     1921 CTAAGGGAGA GGCGGGTGCT CCTGGTGTGA AGGGTGAGAG TGGTTCCCCG GGTGAGAACG 
     1981 GATCTCCGGG CCCAATGGGT CCTCGTGGCC TGCCTGGTGA AAGAGGACGG ACTGGCCCTG 
     2041 CTGGCGCTGC GGGTGCCCGA GGCAACGATG GTCAGCCAGG CCCCGCAGGT CCTCCGGGTC 
     2101 CTGTCGGTCC TGCTGGTGGT CCTGGCTTCC CTGGTGCTCC TGGAGCCAAG GGTGAAGCCG 
     2161 GCCCCACTGG TGCCCGTGGT CCTGAAGGTG CTCAAGGTCC TCGCGGTGAA CCTGGTACTC 
     2221 CTGGGTCCCC TGGGCCTGCT GGTGCCTCCG GTAACCCTGG AACAGATGGA ATTCCTGGAG 
     2281 CCAAAGGATC TGCTGGTGCT CCTGGCATTG CTGGTGCTCC TGGCTTCCCT GGGCCACGGG 
     2341 GTCCTCCTGG CCCTCAAGGT GCAACTGGTC CTCTGGGCCC GAAAGGTCAG ACGGGTGAAC 
     2401 CTGGTATTGC TGGCTTCAAA GGTGAACAAG GCCCCAAGGG AGAACCTGGC CCTGCTGGCC 
     2461 CCCAGGGAGC CCCTGGACCC GCTGGTGAAG AAGGCAAGAG AGGTGCCCGT GGAGAGCCTG 
     2521 GTGGCGTTGG GCCCATCGGT CCCCCTGGAG AAAGAGGTGC TCCCGGAAAC CGCGGTTTCC 
     2581 CAGGTCAAGA TGGTCTGGCA GGTCCCAAGG GAGCCCCTGG AGAGCGAGGG CCCAGTGGTC 
     2641 TTGCTGGCCC CAAGGGAGCC AACGGTGACC CTGGCCGTCC TGGAGAACCT GGCCTTCCTG 
     2701 GAGCCCGGGG TCTCACTGGC CGCCCTGGTG ATGCTGGTCC TCAAGGCAAA GTTGGCCCTT 
     2761 CTGGAGCCCC TGGTGAAGAT GGTCGTCCTG GACCTCCAGG TCCTCAGGGG GCTCGTGGGC 
     2821 AGCCTGGTGT CATGGGTTTC CCTGGCCCCA AAGGTGCCAA CGGTGAGCCT GGCAAAGCTG 
     2881 GTGAGAAGGG ACTGCCTGGT GCTCCTGGTC TGAGGGGTCT TCCTGGCAAA GATGGTGAGA 
     2941 CAGGTGCTGC AGGACCCCCT GGCCCTGCTG GACCTGCTGG TGAACGAGGC GAGCAGGGTG 
     3001 CTCCTGGGCC ATCTGGGTTC CAGGGACTTC CTGGCCCTCC TGGTCCCCCA GGTGAAGGTG 
     3061 GAAAACCAGG TGACCAGGGT GTTCCCGGTG AAGCTGGAGC CCCTGGCCTC GTGGGTCCCA 
     3121 GGGGTGAACG AGGTTTCCCA GGTGAACGTG GCTCTCCCGG TGCCCAGGGC CTCCAGGGTC 
     3181 CCCGTGGCCT CCCCGGCACT CCTGGCACTG ATGGTCCCAA AGGTGCATCT GGCCCAGCAG 
     3241 GCCCCCCTGG CGCACAGGGC CCTCCAGGTC TTCAGGGAAT GCCTGGCGAG AGGGGAGCAG 
     3301 CTGGTATCGC TGGGCCCAAA GGCGACAGGG GTGACGTTGG TGAGAAAGGC CCTGAGGGAG 
     3361 CCCCTGGAAA GGATGGTGGA CGAGGCCTGA CAGGTCCCAT TGGCCCCCCT GGCCCAGCTG 
     3421 GTGCTAACGG CGAGAAGGGA GAAGTTGGAC CTCCTGGTCC TGCAGGAAGT GCTGGTGCTC 
     3481 GTGGCGCTCC GGGTGAACGT GGAGAGACTG GCCCCCCCGG ACCAGCGGGA TTTGCTGGGC 
     3541 CTCCTGGTGC TGATGGCCAG CCTGGGGCCA AGGGTGAGCA AGGAGAGGCC GGCCAGAAAG 
     3601 GCGATGCTGG TGCCCCTGGT CCTCAGGGCC CCTCTGGAGC ACCTGGGCCT CAGGGTCCTA 
     3661 CTGGAGTGAC TGGTCCTAAA GGAGCCCGAG GTGCCCAAGG CCCCCCGGGA GCCACTGGAT 
     3721 TCCCTGGAGC TGCTGGCCGC GTTGGACCCC CAGGCTCCAA TGGCAACCCT GGACCCCCTG 
     3781 GTCCCCCTGG TCCTTCTGGA AAAGATGGTC CCAAAGGTGC TCGAGGAGAC AGCGGCCCCC 
     3841 CTGGCCGAGC TGGTGAACCC GGCCTCCAAG GTCCTGCTGG ACCCCCTGGC GAGAAGGGAG 
     3901 AGCCTGGAGA TGACGGTCCC TCTGGTGCCG AAGGTCCACC AGGTCCCCAG GGTCTGGCTG 
     3961 GTCAGAGAGG CATCGTCGGT CTGCCTGGGC AACGTGGTGA GAGAGGATTC CCTGGCTTGC 
     4021 CTGGCCCATC GGGTGAGCCC GGCAAGCAGG GTGCTCCTGG AGCATCTGGA GACAGAGGTC 
     4081 CTCCTGGCCC CGTGGGTCCT CCTGGCCTGA CGGGTCCTGC AGGTGAACCC GGACGAGAGG 
     4141 GAAGCCCCGG TGCTGATGGC CCCCCTGGCA GAGATGGCGC TGCTGGAGTC AAGGGTGATC 
     4201 GTGGTGAGAC TGGTGCTGTG GGAGCTCCTG GAGCCCCTGG GCCCCCTGGC TCCCCTGGCC 
     4261 CCGCTGGTCC AACTGGCAAG CAAGGAGACA GAGGAGAAGC TGGTGCACAA GGCCCCATGG 
     4321 GACCCTCAGG ACCAGCTGGA GCCCGGGGAA TCCAGGGTCC TCAAGGCCCC AGAGGTGACA 
     4381 AAGGAGAGGC TGGAGAGCCT GGCGAGAGAG GCCTGAAGGG ACACCGTGGC TTCACTGGTC 
     4441 TGCAGGGTCT GCCCGGCCCT CCTGGTCCTT CTGGAGACCA AGGTGCTTCT GGTCCTGCTG 
     4501 GTCCTTCTGG CCCTAGAGGT CCTCCTGGCC CCGTCGGTCC CTCTGGCAAA GATGGTGCTA 
     4561 ATGGAATCCC TGGCCCCATT GGGCCTCCTG GTCCCCGTGG ACGATCAGGC GAAACCGGTC 
     4621 CTGCTGGTCC TCCTGGAAAT CCTGGGCCCC CTGGTCCTCC AGGTCCCCCT GGCCCTGGCA 
     4681 TCGACATGTC CGCCTTTGCT GGCTTAGGCC CGAGAGAGAA GGGCCCCGAC CCCCTGCAGT 
     4741 ACATGCGGGC CGACCAGGCA GCCGGTGGCC TGAGACAGCA TGACGCCGAG GTGGATGCCA 
     4801 CACTCAAGTC CCTCAACAAC CAGATTGAGA GCATCCGCAG CCCCGAGGGC TCCCGCAAGA 
     4861 ACCCTGCTCG CACCTGCAGA GACCTGAAAC TCTGCCACCC TGAGTGGAAG AGTGGAGACT 
     4921 ACTGGATTGA CCCCAACCAA GGCTGCACCT TGGACGCCAT GAAGGTTTTC TGCAACATGG 
     4981 AGACTGGCGA GACTTGCGTC TACCCCAATC CAGCAAACGT TCCCAAGAAG AACTGGTGGA 
     5041 GCAGCAAGAG CAAGGAGAAG AAACACATCT GGTTTGGAGA AACCATCAAT GGTGGCTTCC 
     5101 ATTTCAGCTA TGGAGATGAC AATCTGGCTC CCAACACTGC CAACGTCCAG ATGACCTTCC 
     5161 TACGCCTGCT GTCCACGGAA GGCTCCCAGA ACATCACCTA CCACTGCAAG AACAGCATTG 
     5221 CCTATCTGGA CGAAGCAGCT GGCAACCTCA AGAAGGCCCT GCTCATCCAG GGCTCCAATG 
     5281 ACGTGGAGAT CCGGGCAGAG GGCAATAGCA GGTTCACGTA CACTGCCCTG AAGGATGGCT 
     5341 GCACGAAACA TACCGGTAAG TGGGGCAAGA CTGTTATCGA GTACCGGTCA CAGAAGACCT 
     5401 CACGCCTCCC CATCATTGAC ATTGCACCCA TGGACATAGG AGGGCCCGAG CAGGAATTCG 
     5461 GTGTGGACAT AGGGCCGGTC TGCTTCTTGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5521 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5581 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5641 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5701 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5761 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5821 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5881 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5941 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     6001 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     6061 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     6121 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     6181 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6241 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6301 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6361 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6421 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6481 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6541 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6601 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6661 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6721 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6781 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6841 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6901 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6961 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     7021 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     7081 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     7141 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     7201 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7261 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7321 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7381 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7441 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7501 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7561 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7621 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7681 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7741 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7801 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7861 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7921 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7981 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     8041 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     8101 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     8161 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     8221 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8281 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8341 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8401 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8461 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8521 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8581 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8641 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8701 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8761 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8821 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8881 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8941 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     9001 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     9061 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     9121 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     9181 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9241 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9301 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//