LOCUS       RC217979                8268 bp    DNA     circular  SYN 
DEFINITION  ASAP1 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1)
ACCESSION   NM_018482
VERSION     NM_018482.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4425..4516 
                     /label="Myc-DDK"  
     misc_feature    4546..5131 
                     /label="PolyA signal"  
     misc_feature    4603..4622 
                     /label="Restriction Enzyme"  
     misc_feature    5407..6079 
                     /label="ColE1"  
     misc_feature    6509..7303 
                     /label="Kan/Neo"  
     misc_feature    7338..7640 
                     /label="SV40 ori"  
     misc_feature    7726..8203 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4407..4413 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4407 
                     /label="ASAP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGATCTTCA GCCTCCAGGC TCTCCAGTTT TTCGTCGAGA GATTCACTAT 
     1081 GGAATCGGAT GCCGGACCAG ATCTCTGTCT CGGAGTTCAT CGCCGAGACC ACCGAGGACT 
     1141 ACAACTCGCC CACCACGTCC AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA 
     1201 CGCTGCTGGA GGAGGCTCTA GACCAAGATA GAACAGCCCT TCAGAAAGTG AAGAAGTCTG 
     1261 TAAAAGCAAT ATATAATTCT GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG 
     1321 TTCTTGATAA GTTTGGGAGT AATTTTTTAA GTCGAGACAA CCCCGACCTT GGCACCGCGT 
     1381 TTGTCAAGTT TTCTACTCTT ACAAAGGAAC TGTCCACACT GCTGAAAAAT CTGCTCCAGG 
     1441 GTTTGAGCCA CAATGTGATC TTCACCTTGG ATTCTTTGTT AAAAGGAGAC CTAAAGGGAG 
     1501 TCAAAGGAGA TCTCAAGAAG CCATTTGACA AAGCCTGGAA AGATTATGAG ACAAAGTTTA 
     1561 CAAAAATTGA GAAAGAGAAA AGAGAGCACG CAAAACAACA TGGGATGATC CGCACAGAGA 
     1621 TAACAGGAGC TGAGATTGCG GAAGAAATGG AGAAGGAAAG GCGCCTCTTT CAGCTCCAAA 
     1681 TGTGTGAATA TCTCATTAAA GTTAATGAAA TCAAGACCAA AAAGGGTGTG GATCTGCTGC 
     1741 AGAATCTTAT AAAGTATTAC CATGCACAGT GCAATTTCTT TCAAGATGGC TTGAAAACAG 
     1801 CTGATAAGTT GAAACAGTAC ATTGAAAAAC TGGCTGCTGA TTTATATAAT ATAAAACAGA 
     1861 CCCAGGATGA AGAAAAGAAA CAGCTAACTG CACTCCGAGA CTTAATAAAA TCCTCTCTTC 
     1921 AACTGGATCA GAAAGAAGAT TCTCAGAGCC GGCAAGGAGG ATACAGCATG CATCAGCTCC 
     1981 AGGGCAATAA GGAATATGGC AGTGAAAAGA AGGGGTACCT GCTAAAGAAA AGTGACGGGA 
     2041 TCCGGAAAGT ATGGCAGAGG AGGAAGTGTT CAGTCAAGAA TGGGATTCTG ACCATCTCAC 
     2101 ATGCCACATC TAACAGGCAA CCAGCCAAGT TGAACCTTCT CACCTGCCAA GTAAAACCTA 
     2161 ATGCCGAAGA CAAAAAATCT TTTGACCTGA TATCACATAA TAGAACATAT CACTTTCAGG 
     2221 CAGAAGATGA GCAGGATTAT GTAGCATGGA TATCAGTATT GACAAATAGC AAAGAAGAGG 
     2281 CCCTAACCAT GGCCTTCCGT GGAGAGCAGA GTGCGGGAGA GAACAGCCTG GAAGACCTGA 
     2341 CAAAAGCCAT TATTGAGGAT GTCCAGCGGC TCCCAGGGAA TGACATTTGC TGCGATTGTG 
     2401 GCTCATCAGA ACCCACCTGG CTTTCAACCA ACTTGGGTAT TTTGACCTGT ATAGAATGTT 
     2461 CTGGCATCCA TAGGGAAATG GGGGTTCATA TTTCTCGCAT TCAGTCTTTG GAACTAGACA 
     2521 AATTAGGAAC TTCTGAACTC TTGCTGGCCA AGAATGTAGG AAACAATAGT TTTAATGATA 
     2581 TTATGGAAGC AAATTTACCC AGCCCCTCAC CAAAACCCAC CCCTTCAAGT GATATGACTG 
     2641 TACGAAAAGA ATATATCACT GCAAAGTATG TAGATCATAG GTTTTCAAGG AAGACCTGTT 
     2701 CAACTTCATC AGCTAAACTA AATGAATTGC TTGAGGCCAT CAAATCCAGG GATTTACTTG 
     2761 CACTAATTCA AGTCTATGCA GAAGGGGTAG AGCTAATGGA ACCACTGCTG GAACCTGGGC 
     2821 AGGAGCTTGG GGAGACAGCC CTTCACCTTG CCGTCCGAAC TGCAGATCAG ACATCTCTCC 
     2881 ATTTGGTTGA CTTCCTTGTA CAAAACTGTG GGAACCTGGA TAAGCAGACG GCCCTGGGAA 
     2941 ACACAGTTCT ACACTACTGT AGTATGTACA GTAAACCTGA GTGTTTGAAG CTTTTGCTCA 
     3001 GGAGCAAGCC CACTGTGGAT ATAGTTAACC AGGCTGGAGA AACTGCCCTA GACATAGCAA 
     3061 AGAGACTAAA AGCTACCCAG TGTGAAGATC TGCTTTCCCA GGCTAAATCT GGAAAGTTCA 
     3121 ATCCACACGT CCACGTAGAA TATGAGTGGA ATCTTCGACA GGAGGAGATA GATGAGAGCG 
     3181 ATGATGATCT GGATGACAAA CCAAGCCCTA TCAAGAAAGA GCGCTCACCC AGACCTCAGA 
     3241 GCTTCTGCCA CTCCTCCAGC ATCTCCCCCC AGGACAAGCT GGCACTGCCA GGATTCAGCA 
     3301 CTCCAAGGGA CAAACAGCGG CTCTCCTATG GAGCCTTCAC CAACCAGATC TTCGTTTCCA 
     3361 CAAGCACAGA CTCGCCCACA TCACCAACCA CGGAGGCTCC CCCTCTGCCT CCTAGGAACG 
     3421 CCGGGAAAGG TCCAACTGGC CCACCTTCAA CACTCCCTCT AAGCACCCAG ACCTCTAGTG 
     3481 GCAGCTCCAC CCTATCCAAG AAGAGGCCTC CTCCCCCACC ACCCGGACAC AAGAGAACCC 
     3541 TATCCGACCC TCCCAGCCCA CTACCTCATG GGCCCCCAAA CAAAGGCGCA GTTCCTTGGG 
     3601 GTAACGATGG GGGTCCATCC TCTTCAAGTA AGACTACAAA CAAGTTTGAG GGACTATCCC 
     3661 AGCAGTCGAG CACCAGTTCT GCAAAGACTG CCCTTGGCCC AAGAGTTCTT CCTAAACTAC 
     3721 CTCAGAAAGT GGCACTAAGG AAAACAGATC ATCTCTCCCT AGACAAAGCC ACCATCCCGC 
     3781 CCGAAATCTT TCAGAAATCA TCACAGTTGG CAGAGTTGCC ACAAAAGCCA CCACCTGGAG 
     3841 ACCTGCCCCC AAAGCCCACA GAACTGGCCC CCAAGCCCCA AATTGGAGAT TTGCCGCCTA 
     3901 AGCCAGGAGA ACTGCCCCCC AAACCACAGC TGGGGGACCT GCCACCCAAA CCCCAACTCT 
     3961 CAGACTTACC TCCCAAACCA CAGATGAAGG ACCTGCCCCC CAAACCACAG CTGGGAGACC 
     4021 TGCTAGCAAA ATCCCAGACT GGAGATGTCT CACCCAAGGC TCAGCAACCC TCTGAGGTCA 
     4081 CACTGAAGTC ACACCCATTG GATCTATCCC CAAATGTGCA GTCCAGAGAC GCCATCCAAA 
     4141 AGCAAGCATC TGAAGACTCC AACGACCTCA CGCCTACTCT GCCAGAGACG CCCGTACCAC 
     4201 TGCCCAGAAA AATCAATACG GGGAAAAATA AAGTGAGGCG AGTGAAGACC ATTTATGACT 
     4261 GCCAGGCAGA CAACGATGAC GAGCTCACAT TCATCGAGGG AGAAGTGATT ATCGTCACAG 
     4321 GGGAAGAGGA CCAGGAGTGG TGGATTGGCC ACATCGAAGG ACAGCCTGAA AGGAAGGGGG 
     4381 TCTTTCCAGT GTCCTTTGTT CATATCCTGT CTGACACGCG TACGCGGCCG CTCGAGCAGA 
     4441 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4501 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4561 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4621 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4681 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4741 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4801 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4861 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4921 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4981 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5041 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5101 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5161 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5221 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5281 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5341 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5401 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5461 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5521 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5581 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5641 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5701 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5761 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5821 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5881 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5941 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6001 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6061 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6121 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6181 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6241 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6301 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6361 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6421 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6481 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6541 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6601 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6661 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6721 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6781 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6841 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6901 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6961 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7021 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7081 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7141 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7201 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7261 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7321 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7381 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7441 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7501 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7561 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7621 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7681 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7741 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7801 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7861 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7921 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7981 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8041 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8101 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8161 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8221 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//