LOCUS       RC217795                8895 bp    DNA     circular  SYN 
DEFINITION  PFAS (Myc-DDK-tagged)-Human phosphoribosylformylglycinamidine synthase (PFAS)
ACCESSION   NM_012393
VERSION     NM_012393.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5052..5143 
                     /label="Myc-DDK"  
     misc_feature    5173..5758 
                     /label="PolyA signal"  
     misc_feature    5230..5249 
                     /label="Restriction Enzyme"  
     misc_feature    6034..6706 
                     /label="ColE1"  
     misc_feature    7136..7930 
                     /label="Kan/Neo"  
     misc_feature    7965..8267 
                     /label="SV40 ori"  
     misc_feature    8353..8830 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5034..5040 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5034 
                     /label="PFAS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCCAGTC CTTCACTTCT ATGTTCGTCC CTCTGGCCAT GAGGGGGCAG 
     1081 CCCCTGGACA CACTCGGAGG AAACTGCAAG GGAAACTGCC AGAGCTGCAG GGCGTCGAGA 
     1141 CTGAACTGTG CTACAACGTG AACTGGACAG CTGAGGCCCT CCCCAGTGCT GAGGAGACAA 
     1201 AGAAGCTGAT GTGGCTGTTT GGTTGCCCCT TACTGCTGGA TGATGTTGCT CGGGAGTCCT 
     1261 GGCTCCTTCC TGGCTCCAAT GACCTGCTGC TGGAGGTCGG GCCCAGGCTG AACTTCTCCA 
     1321 CCCCAACATC CACCAACATC GTGTCAGTGT GCCGCGCCAC TGGGCTGGGG CCTGTGGATC 
     1381 GTGTGGAGAC CACCCGGCGC TACCGGCTCT CGTTTGCCCA CCCCCCGTCA GCTGAGGTGG 
     1441 AAGCCATTGC TCTGGCTACC CTGCACGACC GGATGACAGA GCAGCACTTC CCCCATCCCA 
     1501 TCCAGAGTTT CTCCCCTGAG AGCATGCCGG AACCCCTCAA TGGCCCTATC AATATACTGG 
     1561 GTGAGGGCCG GCTTGCGCTG GAGAAGGCCA ACCAGGAGCT TGGTCTGGCT TTAGACTCTT 
     1621 GGGACCTAGA CTTCTACACC AAGCGCTTCC AGGAGCTACA GCGGAACCCG AGCACTGTGG 
     1681 AGGCCTTTGA CTTGGCGCAG TCCAATAGCG AGCACAGCCG ACACTGGTTC TTCAAGGGCC 
     1741 AGCTCCACGT GGATGGGCAG AAGCTGGTGC ACTCACTGTT TGAGTCCATC ATGAGCACCC 
     1801 AGGAATCCTC GAACCCCAAC AACGTCCTCA AATTCTGTGA TAACAGCAGT GCAATCCAGG 
     1861 GAAAGGAAGT CCGATTCCTA CGGCCTGAGG ACCCCACACG GCCAAGCCGC TTCCAGCAAC 
     1921 AGCAAGGGCT GAGACATGTT GTCTTCACAG CAGAGACTCA CAACTTTCCC ACAGGAGTAT 
     1981 GCCCCTTTAG TGGTGCAACC ACTGGCACAG GGGGCCGGAT TCGAGATGTC CAGTGCACAG 
     2041 GCCGCGGGGC CCACGTGGTG GCTGGCACTG CCGGCTATTG CTTTGGAAAT CTGCATATTC 
     2101 CAGGTTACAA TCTGCCCTGG GAGGATCCAA GCTTCCAGTA TCCTGGGAAT TTTGCCCGGC 
     2161 CCCTGGAGGT TGCCATTGAA GCCAGTAATG GAGCTTCTGA CTATGGCAAC AAGTTTGGGG 
     2221 AACCAGTGCT GGCTGGCTTC GCCCGCTCCT TGGGCCTCCA GCTCCCAGAC GGCCAGCGGC 
     2281 GTGAGTGGAT CAAGCCCATC ATGTTTAGTG GGGGCATTGG GTCCATGGAA GCTGACCACA 
     2341 TAAGCAAGGA GGCCCCAGAG CCAGGCATGG AAGTTGTAAA GGTTGGAGGT CCCGTCTACA 
     2401 GGATTGGAGT TGGAGGTGGA GCTGCTTCAT CTGTGCAGGT GCAGGGAGAT AACACCAGTG 
     2461 ACCTGGACTT TGGGGCTGTG CAGCGAGGAG ACCCGGAGAT GGAACAGAAG ATGAACCGTG 
     2521 TGATCAGGGC TTGTGTGGAG GCCCCCAAGG GAAACCCCAT CTGCAGCCTT CATGATCAGG 
     2581 GCGCTGGTGG CAATGGCAAT GTCCTAAAAG AGCTGAGTGA CCCAGCTGGA GCCATCATTT 
     2641 ACACCAGCCG CTTCCAGCTT GGGGACCCAA CCCTGAATGC CCTGGAAATC TGGGGGGCTG 
     2701 AGTACCAGGA ATCAAATGCT CTTCTGCTGA GGTCCCCCAA CCGGGACTTC CTGACTCATG 
     2761 TCAGTGCCCG TGAACGTTGC CCGGCTTGCT TCGTGGGCAC CATCACTGGA GACCGGAGAA 
     2821 TAGTGCTGGT GGACGATCGG GAGTGTCCTG TCAGAAGAAA TGGCCAGGGG GATGCCCCCC 
     2881 CGACACCCCT GCCAACCCCT GTGGACCTGG AGCTCGAATG GGTGCTGGGC AAGATGCCTC 
     2941 GGAAGGAGTT CTTCCTGCAG AGGAAGCCCC CCATGCTGCA GCCTCTGGCC TTGCCCCCAG 
     3001 GGCTGAGCGT GCACCAGGCT CTGGAGAGGG TTCTGAGGCT GCCCGCCGTG GCCAGCAAGC 
     3061 GCTACCTCAC CAATAAGGTG GACCGCTCTG TGGGAGGCCT GGTGGCCCAG CAGCAGTGCG 
     3121 TGGGGCCCCT GCAAACTCCT CTGGCAGATG TAGCGGTTGT GGCACTGAGC CATGAGGAGC 
     3181 TCATAGGGGC TGCCACAGCC TTGGGAGAAC AGCCAGTCAA GAGCCTGCTG GACCCAAAAG 
     3241 TCGCCGCCCG GCTGGCCGTG GCCGAAGCCC TCACCAACCT GGTGTTTGCT CTGGTCACTG 
     3301 ACCTCCGGGA TGTGAAGTGT AGCGGGAACT GGATGTGGGC AGCCAAGCTC CCAGGGGAGG 
     3361 GCGCAGCTTT GGCGGATGCC TGTGAGGCTA TGGTGGCAGT GATGGCAGCC CTGGGTGTGG 
     3421 CAGTGGATGG TGGCAAGGAC TCCCTCAGCA TGGCTGCTCG GGTTGGCACT GAGACCGTGC 
     3481 GGGCTCCTGG GTCACTGGTC ATCTCAGCCT ATGCCGTCTG CCCAGACATC ACAGCCACTG 
     3541 TGACCCCAGA CCTCAAGCAT CCTGAAGGGA GAGGCCATCT GCTCTATGTG GCTCTGAGCC 
     3601 CTGGGCAGCA CCGGCTCGGG GGCACAGCTC TGGCCCAGTG CTTCTCCCAG CTTGGGGAAC 
     3661 ACCCTCCAGA CCTGGACCTT CCTGAGAACT TGGTGCGGGC CTTCAGCATC ACTCAGGGGC 
     3721 TGCTGAAAGA CCGCCTCCTC TGCTCAGGCC ACGATGTCAG TGACGGAGGC CTCGTCACAT 
     3781 GCCTGCTGGA GATGGCCTTT GCTGGAAATT GCGGGCTACA GGTGGATGTG CCTGTCCCCA 
     3841 GGGTTGATGT CCTGTCTGTG CTGTTCGCTG AGGAGCCAGG CCTCGTGCTG GAGGTGCAGG 
     3901 AGCCAGACCT GGCCCAGGTG CTGAAGCGTT ACCGGGATGC TGGCCTCCAT TGCCTGGAGC 
     3961 TGGGCCACAC AGGCGAGGCC GGGCCCCACG CCATGGTCCG GGTGTCAGTG AACGGGGCTG 
     4021 TGGTTCTGGA GGAGCCTGTT GGGGAGCTGC GAGCCCTCTG GGAGGAGACG AGTTTCCAGC 
     4081 TGGACCGGCT ACAGGCAGAG CCTCGCTGTG TGGCAGAGGA GGAACGGGGC CTGAGGGAGC 
     4141 GGATGGGGCC CAGCTATTGC CTGCCCCCCA CCTTTCCCAA AGCCTCCGTG CCCCGTGAGC 
     4201 CTGGTGGTCC CAGCCCCCGA GTCGCCATCT TGCGAGAGGA GGGCAGTAAT GGAGACCGGG 
     4261 AGATGGCCGA TGCCTTCCAC TTAGCTGGGT TTGAGGTATG GGACGTGACC ATGCAGGACC 
     4321 TCTGCTCTGG GGCAATTGGG CTGGACACTT TCCGTGGCGT GGCCTTCGTG GGCGGCTTCA 
     4381 GCTATGCAGA TGTCCTGGGC TCTGCCAAAG GGTGGGCAGC TGCTGTGACC TTTCATCCCA 
     4441 GGGCTGGGGC TGAGCTGAGG CGCTTCCGGA AGCGGCCAGA CACCTTCAGC CTGGGCGTGT 
     4501 GTAATGGCTG TCAACTGCTG GCTCTGCTCG GCTGGGTGGG AGGCGACCCC AATGAGGATG 
     4561 CTGCAGAGAT GGGCCCTGAC TCCCAGCCAG CCCGGCCAGG CCTTCTGCTA CGCCACAACC 
     4621 TGTCTGGGCG CTACGAGTCT CGCTGGGCCA GCGTGCGTGT GGGGCCTGGG CCAGCCCTGA 
     4681 TGCTGCGAGG GATGGAGGGC GCCGTGCTGC CCGTGTGGAG TGCGCACGGG GAAGGTTACG 
     4741 TAGCATTTTC TTCTCCGGAA CTCCAAGCTC AGATTGAGGC CAGGGGCTTG GCTCCACTGC 
     4801 ACTGGGCTGA TGATGACGGG AACCCCACAG AGCAGTACCC TCTGAATCCC AATGGGTCCC 
     4861 CAGGGGGCGT GGCTGGCATC TGCTCCTGTG ATGGCCGCCA CCTGGCTGTC ATGCCTCACC 
     4921 CTGAGCGGGC CGTTAGGCCT TGGCAGTGGG CATGGCGACC CCCTCCATTT GATACTCTGA 
     4981 CCACCTCCCC CTGGCTCCAG CTCTTTATCA ATGCCCGAAA CTGGACCCTG GAAGGGAGCT 
     5041 GCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5101 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5161 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5221 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5281 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5341 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5401 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5461 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5521 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5581 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5641 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5701 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5761 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5821 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5881 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5941 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6001 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6061 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6121 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6181 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6241 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6301 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6361 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6421 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6481 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6541 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6601 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6661 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6721 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6781 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6841 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6901 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6961 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7021 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7081 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7141 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7201 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7261 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7321 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7381 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7441 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7501 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7561 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7621 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7681 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7741 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7801 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7861 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7921 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7981 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8041 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8101 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8161 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8221 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8281 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8341 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8401 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8461 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8521 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8581 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8641 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8701 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8761 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8821 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8881 TTTAACGCGA ATTTT
//