LOCUS       RC217720                8703 bp    DNA     circular  SYN 
DEFINITION  MYBPC3 (Myc-DDK-tagged)-Human myosin binding protein C, cardiac (MYBPC3)
ACCESSION   NM_000256
VERSION     NM_000256.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4860..4951 
                     /label="Myc-DDK"  
     misc_feature    4981..5566 
                     /label="PolyA signal"  
     misc_feature    5038..5057 
                     /label="Restriction Enzyme"  
     misc_feature    5842..6514 
                     /label="ColE1"  
     misc_feature    6944..7738 
                     /label="Kan/Neo"  
     misc_feature    7773..8075 
                     /label="SV40 ori"  
     misc_feature    8161..8638 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4842..4848 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4842 
                     /label="MYBPC3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGAGCCG GGGAAGAAGC CAGTCTCAGC CTTTAGCAAG AAGCCACGGT 
     1081 CAGTGGAAGT GGCCGCAGGC AGCCCTGCCG TGTTCGAGGC CGAGACAGAG CGGGCAGGAG 
     1141 TGAAGGTGCG CTGGCAGCGC GGAGGCAGTG ACATCAGCGC CAGCAACAAG TACGGCCTGG 
     1201 CCACAGAGGG CACACGGCAT ACGCTGACAG TGCGGGAAGT GGGCCCTGCC GACCAGGGAT 
     1261 CTTACGCAGT CATTGCTGGC TCCTCCAAGG TCAAGTTCGA CCTCAAGGTC ATAGAGGCAG 
     1321 AGAAGGCAGA GCCCATGCTG GCCCCTGCCC CTGCCCCTGC TGAGGCCACT GGAGCCCCTG 
     1381 GAGAAGCCCC GGCCCCAGCC GCTGAGCTGG GAGAAAGTGC CCCAAGTCCC AAAGGGTCAA 
     1441 GCTCAGCAGC TCTCAATGGT CCTACCCCTG GAGCCCCCGA TGACCCCATT GGCCTCTTCG 
     1501 TGATGCGGCC ACAGGATGGC GAGGTGACCG TGGGTGGCAG CATCACCTTC TCAGCCCGCG 
     1561 TGGCCGGCGC CAGCCTCCTG AAGCCGCCTG TGGTCAAGTG GTTCAAGGGC AAATGGGTGG 
     1621 ACCTGAGCAG CAAGGTGGGC CAGCACCTGC AGCTGCACGA CAGCTACGAC CGCGCCAGCA 
     1681 AGGTCTATCT GTTCGAGCTG CACATCACCG ATGCCCAGCC TGCCTTCACT GGCAGCTACC 
     1741 GCTGTGAGGT GTCCACCAAG GACAAATTTG AATGCTCCAA CTTCAATCTC ACTGTCCACG 
     1801 AGGCCATGGG CACCGGAGAC CTGGACCTCC TATCAGCCTT CCGCCGCACG AGCCTGGCTG 
     1861 GAGGTGGTCG GCGGATCAGT GATAGCCATG AGGACACTGG GATTCTGGAC TTCAGCTCAC 
     1921 TGCTGAAAAA GAGAGACAGT TTCCGGACCC CGAGGGACTC GAAGCTGGAG GCACCAGCAG 
     1981 AGGAGGACGT GTGGGAGATC CTACGGCAGG CACCCCCATC TGAGTACGAG CGCATCGCCT 
     2041 TCCAGTACGG CGTCACTGAC CTGCGCGGCA TGCTAAAGAG GCTCAAGGGC ATGAGGCGCG 
     2101 ATGAGAAGAA GAGCACAGCC TTTCAGAAGA AGCTGGAGCC GGCCTACCAG GTGAGCAAAG 
     2161 GCCACAAGAT CCGGCTGACC GTGGAACTGG CTGACCATGA CGCTGAGGTC AAATGGCTCA 
     2221 AGAATGGCCA GGAGATCCAG ATGAGCGGCA GCAAGTACAT CTTTGAGTCC ATCGGTGCCA 
     2281 AGCGTACCCT GACCATCAGC CAGTGCTCAT TGGCGGACGA CGCAGCCTAC CAGTGCGTGG 
     2341 TGGGTGGCGA GAAGTGTAGC ACGGAGCTCT TTGTGAAAGA GCCCCCTGTG CTCATCACGC 
     2401 GCCCCTTGGA GGACCAGCTG GTGATGGTGG GGCAGCGGGT GGAGTTTGAG TGTGAAGTAT 
     2461 CGGAGGAGGG GGCGCAAGTC AAATGGCTGA AGGACGGGGT GGAGCTGACC CGGGAGGAGA 
     2521 CCTTCAAATA CCGGTTCAAG AAGGACGGGC AGAGACACCA CCTGATCATC AACGAGGCCA 
     2581 TGCTGGAGGA CGCGGGGCAC TATGCACTGT GCACTAGCGG GGGCCAGGCG CTGCGTGAGC 
     2641 TCATTGTGCA GGAAAAGAAG CTGGAGGTGT ACCAGAGCAT CGCAGACCTG ATGGTGGGCG 
     2701 CAAAGGACCA GGCGGTGTTC AAATGTGAGG TCTCAGATGA GAATGTTCGG GGTGTGTGGC 
     2761 TGAAGAATGG GAAGGAGCTG GTGCCCGACA GCCGCATAAA GGTGTCCCAC ATCGGGCGGG 
     2821 TCCACAAACT GACCATTGAC GACGTCACAC CTGCCGACGA GGCTGACTAC AGCTTTGTGC 
     2881 CCGAGGGCTT CGCCTGCAAC CTGTCAGCCA AGCTCCACTT CATGGAGGTC AAGATTGACT 
     2941 TCGTACCCAG GCAGGAACCT CCCAAGATCC ACCTGGACTG CCCAGGCCGC ATACCAGACA 
     3001 CCATTGTGGT TGTAGCTGGA AATAAGCTAC GTCTGGACGT CCCTATCTCT GGGGACCCTG 
     3061 CTCCCACTGT GATCTGGCAG AAGGCTATCA CGCAGGGGAA TAAGGCCCCA GCCAGGCCAG 
     3121 CCCCAGATGC CCCAGAGGAC ACAGGTGACA GCGATGAGTG GGTGTTTGAC AAGAAGCTGC 
     3181 TGTGTGAGAC CGAGGGCCGG GTCCGCGTGG AGACCACCAA GGACCGCAGC ATCTTCACGG 
     3241 TCGAGGGGGC AGAGAAGGAA GATGAGGGCG TCTACACGGT CACAGTGAAG AACCCTGTGG 
     3301 GCGAGGACCA GGTCAACCTC ACAGTCAAGG TCATCGACGT GCCAGACGCA CCTGCGGCCC 
     3361 CCAAGATCAG CAACGTGGGA GAGGACTCCT GCACAGTACA GTGGGAGCCG CCTGCCTACG 
     3421 ATGGCGGGCA GCCCATCCTG GGCTACATCC TGGAGCGCAA GAAGAAGAAG AGCTACCGGT 
     3481 GGATGCAGCT GAACTTCGAC CTGATTCAGG AGCTGAGTCA TGAAGCGCGG CGCATGATCG 
     3541 AGGGCGTGGT GTACGAGATG CGCGTCTACG CGGTCAACGC CATCGGCATG TCCAGGCCCA 
     3601 GCCCTGCCTC CCAGCCCTTC ATGCCTATCG GTCCCCCCAG CGAACCCACC CACCTGGCAG 
     3661 TAGAGGACGT CTCTGACACC ACGGTCTCCC TCAAGTGGCG GCCCCCAGAG CGCGTGGGAG 
     3721 CAGGAGGCCT GGATGGCTAC AGCGTGGAGT ACTGCCCAGA GGGCTGCTCA GAGTGGGTGG 
     3781 CTGCCCTGCA GGGGCTGACA GAGCACACAT CGATACTGGT GAAGGACCTG CCCACGGGGG 
     3841 CCCGGCTGCT TTTCCGAGTG CGGGCACACA ATATGGCAGG GCCTGGAGCC CCTGTTACCA 
     3901 CCACGGAGCC GGTGACAGTG CAGGAGATCC TGCAACGGCC ACGGCTTCAG CTGCCCAGGC 
     3961 ACCTGCGCCA GACCATTCAG AAGAAGGTCG GGGAGCCTGT GAACCTTCTC ATCCCTTTCC 
     4021 AGGGCAAGCC CCGGCCTCAG GTGACCTGGA CCAAAGAGGG GCAGCCCCTG GCAGGCGAGG 
     4081 AGGTGAGCAT CCGCAACAGC CCCACAGACA CCATCCTGTT CATCCGGGCC GCTCGCCGCG 
     4141 TGCATTCAGG CACTTACCAG GTGACGGTGC GCATTGAGAA CATGGAGGAC AAGGCCACGC 
     4201 TGGTGCTGCA GGTTGTTGAC AAGCCAAGTC CTCCCCAGGA TCTCCGGGTG ACTGACGCCT 
     4261 GGGGTCTTAA TGTGGCTCTG GAGTGGAAGC CACCCCAGGA TGTCGGCAAC ACGGAACTCT 
     4321 GGGGGTACAC AGTGCAGAAA GCCGACAAGA AGACCATGGA GTGGTTCACC GTCTTGGAGC 
     4381 ATTACCGCCG CACCCACTGC GTGGTGCCAG AGCTCATCAT TGGCAATGGC TACTACTTCC 
     4441 GCGTCTTCAG CCAGAATATG GTTGGCTTTA GTGACAGAGC GGCCACCACC AAGGAGCCCG 
     4501 TCTTTATCCC CAGACCAGGC ATCACCTATG AGCCACCCAA CTATAAGGCC CTGGACTTCT 
     4561 CCGAGGCCCC AAGCTTCACC CAGCCCCTGG TGAACCGCTC GGTCATCGCG GGCTACACTG 
     4621 CTATGCTCTG CTGTGCTGTC CGGGGTAGCC CCAAGCCCAA GATTTCCTGG TTCAAGAATG 
     4681 GCCTGGACCT GGGAGAAGAC GCCCGCTTCC GCATGTTCAG CAAGCAGGGA GTGTTGACTC 
     4741 TGGAGATTAG AAAGCCCTGC CCCTTTGACG GGGGCATCTA TGTCTGCAGG GCCACCAACT 
     4801 TACAGGGCGA GGCACGGTGT GAGTGCCGCC TGGAGGTGCG AGTGCCTCAG ACGCGTACGC 
     4861 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4921 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4981 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5041 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5101 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5161 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5221 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5281 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5341 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5401 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5461 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5521 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5581 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5641 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5701 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5761 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5821 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5881 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5941 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6001 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6061 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6121 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6181 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6241 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6301 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6361 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6421 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6481 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6541 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6601 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6661 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6721 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6781 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6841 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6901 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6961 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7021 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7081 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7141 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7201 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7261 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7321 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7381 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7441 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7501 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7561 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7621 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7681 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7741 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7801 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7861 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7921 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7981 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8041 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8101 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8161 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8221 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8281 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8341 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8401 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8461 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8521 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8581 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8641 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8701 TTT
//