LOCUS       RC217684L1                9276 bp    DNA     circular  SYN 
DEFINITION  PTK2B (Myc-DDK-tagged)-Human PTK2B protein tyrosine kinase 2 beta (PTK2B), transcript variant 4
ACCESSION   NM_173175
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7567..7747 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8475..9134 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7906..8474 
                     /label="ColE1"  
     misc_feature    6442..6462 
                     /label="LR50 primer"  
     misc_feature    6239..6310 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6451..7042 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6221..6227 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6221 
                     /label="PTK2B"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTCTGGGGTG TCCGAGCCCC TGAGTCGAGT 
     3361 AAAGTTGGGC ACGTTACGCC GGCCTGAAGG CCCTGCAGAG CCCATGGTGG TGGTACCAGT 
     3421 AGATGTGGAA AAGGAGGACG TGCGTATCCT CAAGGTCTGC TTCTATAGCA ACAGCTTCAA 
     3481 TCCTGGGAAA AACTTCAAAC TGGTCAAATG CACTGTCCAG ACGGAGATCC GGGAGATCAT 
     3541 CACCTCCATC CTGCTGAGCG GGCGGATCGG GCCCAACATC CGGTTGGCTG AGTGCTATGG 
     3601 GCTGAGGCTG AAGCACATGA AGTCCGATGA GATCCACTGG CTGCACCCAC AGATGACGGT 
     3661 GGGTGAGGTG CAGGACAAGT ATGAGTGTCT GCACGTGGAA GCCGAGTGGA GGTATGACCT 
     3721 TCAAATCCGC TACTTGCCAG AAGACTTCAT GGAGAGCCTG AAGGAGGACA GGACCACGCT 
     3781 GCTCTATTTT TACCAACAGC TCCGGAACGA CTACATGCAG CGCTACGCCA GCAAGGTCAG 
     3841 CGAGGGCATG GCCCTGCAGC TGGGCTGCCT GGAGCTCAGG CGGTTCTTCA AGGATATGCC 
     3901 CCACAATGCA CTTGACAAGA AGTCCAACTT CGAGCTCCTA GAAAAGGAAG TGGGGCTGGA 
     3961 CTTGTTTTTC CCAAAGCAGA TGCAGGAGAA CTTAAAGCCC AAACAGTTCC GGAAGATGAT 
     4021 CCAGCAGACC TTCCAGCAGT ACGCCTCGCT CAGGGAGGAG GAGTGCGTCA TGAAGTTCTT 
     4081 CAACACTCTC GCCGGCTTCG CCAACATCGA CCAGGAGACC TACCGCTGTG AACTCATTCA 
     4141 AGGATGGAAC ATTACTGTGG ACCTGGTCAT TGGCCCTAAA GGGATCCGCC AGCTGACTAG 
     4201 TCAGGACGCA AAGCCCACCT GCCTGGCCGA GTTCAAGCAG ATCAGGTCCA TCAGGTGCCT 
     4261 CCCGCTGGAG GAGGGCCAGG CAGTACTTCA GCTGGGCATT GAAGGTGCCC CCCAGGCCTT 
     4321 GTCCATCAAA ACCTCATCCC TAGCAGAGGC TGAGAACATG GCTGACCTCA TAGACGGCTA 
     4381 CTGCCGGCTG CAGGGTGAGC ACCAAGGCTC TCTCATCATC CATCCTAGGA AAGATGGTGA 
     4441 GAAGCGGAAC AGCCTGCCCC AGATCCCCAT GCTAAACCTG GAGGCCCGGC GGTCCCACCT 
     4501 CTCAGAGAGC TGCAGCATAG AGTCAGACAT CTACGCAGAG ATTCCCGACG AAACCCTGCG 
     4561 AAGGCCCGGA GGTCCACAGT ATGGCATTGC CCGTGAAGAT GTGGTCCTGA ATCGTATTCT 
     4621 TGGGGAAGGC TTTTTTGGGG AGGTCTATGA AGGTGTCTAC ACAAATCACA AAGGGGAGAA 
     4681 AATCAATGTA GCTGTCAAGA CCTGCAAGAA AGACTGCACT CTGGACAACA AGGAGAAGTT 
     4741 CATGAGCGAG GCAGTGATCA TGAAGAACCT CGACCACCCG CACATCGTGA AGCTGATCGG 
     4801 CATCATTGAA GAGGAGCCCA CCTGGATCAT CATGGAATTG TATCCCTATG GGGAGCTGGG 
     4861 CCACTACCTG GAGCGGAACA AGAACTCCCT GAAGGTGCTC ACCCTCGTGC TGTACTCACT 
     4921 GCAGATATGC AAAGCCATGG CCTACCTGGA GAGCATCAAC TGCGTGCACA GGGACATTGC 
     4981 TGTCCGGAAC ATCCTGGTGG CCTCCCCTGA GTGTGTGAAG CTGGGGGACT TTGGTCTTTC 
     5041 CCGGTACATT GAGGACGAGG ACTATTACAA AGCCTCTGTG ACTCGTCTCC CCATCAAATG 
     5101 GATGTCCCCA GAGTCCATTA ACTTCCGACG CTTCACGACA GCCAGTGACG TCTGGATGTT 
     5161 CGCCGTGTGC ATGTGGGAGA TCCTGAGCTT TGGGAAGCAG CCCTTCTTCT GGCTGGAGAA 
     5221 CAAGGATGTC ATCGGGGTGC TGGAGAAAGG AGACCGGCTG CCCAAGCCTG ATCTCTGTCC 
     5281 ACCGGTCCTT TATACCCTCA TGACCCGCTG CTGGGACTAC GACCCCAGTG ACCGGCCCCG 
     5341 CTTCACCGAG CTGGTGTGCA GCCTCAGTGA CGTTTATCAG ATGGAGAAGG ACATTGCCAT 
     5401 GGAGCAAGAG AGGAATGCTC GCTACCGAAC CCCCAAAATC TTGGAGCCCA CAGCCTTCCA 
     5461 GGAACCCCCA CCCAAGCCCA GCCGACCTAA GTACAGACCC CCTCCGCAAA CCAACCTCCT 
     5521 GGCTCCAAAG CTGCAGTTCC AGGAGGAGGA CTTCATCCAA CCCAGCAGCC GAGAAGAGGC 
     5581 CCAGCAGCTG TGGGAGGCTG AAAAGGTCAA AATGCGGCAA ATCCTGGACA AACAGCAGAA 
     5641 GCAGATGGTG GAGGACTACC AGTGGCTCAG GCAGGAGGAG AAGTCCCTGG ACCCCATGGT 
     5701 TTATATGAAT GATAAGTCCC CATTGACGCC AGAGAAGGAG GTCGGCTACC TGGAGTTCAC 
     5761 AGGGCCCCCA CAGAAGCCCC CGAGGCTGGG CGCACAGTCC ATCCAGCCCA CAGCTAACCT 
     5821 GGACCGGACT GATGACCTGG TGTACCTCAA TGTCATGGAG CTGGTGTGGG CCGTGCTGGA 
     5881 GCTCAAGAAT GAGCTCTGTC AGCTGCCCCC CGAGGGCTAC GTGGTGGTGG TGAAGAATGT 
     5941 GGGGCTGACC CTGCGGAAGC TCATCGGGAG CGTGGATGAT CTCCTGCCTT CCTTGCCGTC 
     6001 ATCTTCACGG ACAGAGATCG AGGGCACCCA GAAACTGCTC AACAAAGACC TGGCAGAGCT 
     6061 CATCAACAAG ATGCGGCTGG CACAGCAGAA CGCCGTGACC TCCCTAAGTG AGGAGTGCAA 
     6121 GAGGCAGATG CTGACGGCTT CACACACCCT GGCTGTGGAC GCCAAGAACC TGCTCGACGC 
     6181 TGTGGACCAG GCCAAGGTTC TGGCCAATCT GGCCCACCCA CCTGCAGAGA CGCGTACGCG 
     6241 GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA 
     6301 CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CTGTACAAGT AGGATTCGTC 
     6361 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATACATGT TTAAGGGTTC 
     6421 CGGTTCCACT AGGTACAATT CGATATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA 
     6481 ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC 
     6541 GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC 
     6601 TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT 
     6661 GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC 
     6721 TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC 
     6781 GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG 
     6841 GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT 
     6901 CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC 
     6961 CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT 
     7021 CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC GACCTCGATC GAGACCTAGA 
     7081 AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT 
     7141 AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA CCTCAGGTAC CTTTAAGACC 
     7201 AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA 
     7261 AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA 
     7321 AGGCTACTTC CCTGATTGGC AGAACTACAC ACCAGGGCCA GGGATCAGAT ATCCACTGAC 
     7381 CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCAAGAG AAGGTAGAAG AAGCCAATGA 
     7441 AGGAGAGAAC ACCCGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG 
     7501 AGAAGTATTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACATGG CCCGAGAGCT 
     7561 GCATCCGGAC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG 
     7621 CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT 
     7681 GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT 
     7741 GTGGAAAATC TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     7801 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     7861 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     7921 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     7981 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     8041 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     8101 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     8161 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     8221 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     8281 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     8341 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     8401 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     8461 ACGTTAAGGG ATTTTGGTCA TGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT 
     8521 AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC 
     8581 GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG 
     8641 GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA 
     8701 TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA 
     8761 CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT 
     8821 TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA 
     8881 ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAACTC CGGATGAGCA 
     8941 TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT 
     9001 ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA 
     9061 ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA 
     9121 TATCCAGTGA TTTTTTTCTC CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     9181 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     9241 GTCCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
//