LOCUS       RC217677                8604 bp    DNA     circular  SYN 
DEFINITION  NPHS1 (Myc-DDK-tagged)-Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1)
ACCESSION   NM_004646
VERSION     NM_004646.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4761..4852 
                     /label="Myc-DDK"  
     misc_feature    4882..5467 
                     /label="PolyA signal"  
     misc_feature    4939..4958 
                     /label="Restriction Enzyme"  
     misc_feature    5743..6415 
                     /label="ColE1"  
     misc_feature    6845..7639 
                     /label="Kan/Neo"  
     misc_feature    7674..7976 
                     /label="SV40 ori"  
     misc_feature    8062..8539 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4743..4749 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4743 
                     /label="NPHS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTGGGG ACGACGCTCA GGGCTTCTCT CCTGCTCCTG GGGCTGCTGA 
     1081 CTGAAGGCCT GGCGCAGTTG GCGATTCCTG CCTCCGTTCC CCGGGGCTTC TGGGCCCTGC 
     1141 CTGAAAACCT GACGGTGGTG GAGGGGGCCT CAGTGGAGCT GCGTTGTGGG GTCAGCACCC 
     1201 CTGGCAGTGC GGTGCAATGG GCCAAAGATG GGCTGCTCCT GGGCCCCGAC CCCAGGATCC 
     1261 CAGGCTTCCC GAGGTACCGC CTGGAAGGGG ACCCTGCTAG AGGTGAATTC CACCTGCACA 
     1321 TCGAGGCCTG TGACCTCAGC GATGACGCGG AGTATGAGTG CCAGGTCGGC CGCTCTGAGA 
     1381 TGGGGCCCGA GCTCGTGTCT CCCAGAGTGA TCCTCTCCAT CCTGGTTCCT CCCAAGCTGC 
     1441 TCCTGCTGAC CCCAGAGGCA GGCACCATGG TCACCTGGGT AGCTGGGCAG GAGTACGTGG 
     1501 TCAACTGTGT GTCTGGGGAC GCGAAGCCAG CACCTGACAT CACCATTCTC CTGAGTGGAC 
     1561 AGACAATATC TGACATCTCT GCAAACGTGA ACGAGGGCTC CCAGCAGAAA CTCTTCACTG 
     1621 TGGAGGCCAC AGCCAGGGTG ACACCCCGGA GCTCAGATAA TAGGCAGTTG CTGGTCTGTG 
     1681 AGGCGTCTAG CCCAGCACTG GAGGCCCCCA TCAAGGCCTC ATTCACCGTG AATGTTCTGT 
     1741 TCCCTCCAGG ACCCCCTGTC ATCGAGTGGC CAGGCCTGGA TGAGGGGCAC GTGCGGGCAG 
     1801 GACAGAGCTT GGAGCTGCCG TGCGTGGCCC GAGGGGGTAA TCCCTTAGCC ACACTGCAGT 
     1861 GGCTGAAGAA TGGCCAGCCG GTGTCCACAG CGTGGGGCAC AGAGCACACC CAGGCGGTGG 
     1921 CCCGCAGTGT GCTGGTGATG ACCGTGAGGC CAGAAGACCA TGGAGCGCAG CTCAGCTGCG 
     1981 AGGCCCACAA CAGCGTGTCT GCAGGGACCC AGGAGCACGG CATCACACTG CAGGTCACCT 
     2041 TTCCCCCTAG TGCCATTATT ATCTTGGGAT CTGCATCCCA GACTGAGAAC AAGAACGTGA 
     2101 CACTCTCCTG TGTCAGCAAG TCCAGTCGCC CGCGGGTTCT GCTACGATGG TGGCTGGGCT 
     2161 GGCGGCAGCT GCTGCCCATG GAGGAGACAG TCATGGATGG ACTGCATGGC GGTCACATCT 
     2221 CCATGTCCAA CCTGACATTC CTGGCGCGGC GGGAGGACAA CGGTCTGACC CTCACATGTG 
     2281 AGGCCTTCAG TGAAGCCTTC ACCAAGGAGA CCTTCAAGAA GTCGCTCATC CTGAACGTAA 
     2341 AATATCCCGC CCAGAAACTG TGGATTGAGG GTCCCCCAGA GGGCCAGAAG CTCCGGGCTG 
     2401 GGACCCGGGT GAGGCTGGTG TGTTTGGCTA TCGGGGGCAA CCCAGAGCCC TCCCTCATGT 
     2461 GGTACAAGGA CTCGCGCACC GTGACCGAGT CGCGGCTGCC GCAGGAGTCG CGGCGCGTGC 
     2521 ATCTCGGCAG CGTGGAGAAA TCTGGGAGCA CCTTCTCCCG AGAGCTGGTG CTGGTCACAG 
     2581 GGCCGTCGGA CAACCAGGCC AAGTTCACGT GCAAGGCTGG ACAGCTCAGC GCGTCCACGC 
     2641 AGCTGGCGGT GCAGTTTCCC CCAACTAACG TGACGATCCT GGCCAACGCA TCCGCACTGC 
     2701 GCCCGGGAGA CGCCTTAAAC TTGACATGCG TCAGCGTCAG CAGCAATCCG CCGGTCAACT 
     2761 TGTCCTGGGA CAAGGAAGGG GAGAGGCTGG AGGGCGTGGC CGCCCCACCC CGGAGAGCCC 
     2821 CATTCAAAGG CTCCGCCGCC GCCAGGAGCG TCCTTCTGCA AGTGTCATCC CGCGATCATG 
     2881 GCCAGCGCGT GACCTGCCGC GCCCACAGCG CCGAGCTCCG CGAAACCGTG AGCTCCTTCT 
     2941 ATCGCCTCAA CGTACTGTAC CGTCCAGAGT TCCTGGGGGA GCAGGTGCTG GTGGTGACCG 
     3001 CGGTGGAGCA GGGCGAGGCG TTGCTGCCCG TGTCCGTGTC CGCTAACCCC GCCCCCGAGG 
     3061 CCTTCAACTG GACCTTCCGC GGCTATCGCC TCAGTCCAGC GGGCGGCCCC CGGCATCGCA 
     3121 TCCTGTCCAG CGGGGCTCTG CATCTGTGGA ATGTGACCCG CGCGGACGAC GGCCTCTATC 
     3181 AGCTGCACTG CCAGAACTCT GAGGGCACCG CGGAAGCGCG GCTGCGGCTG GACGTGCACT 
     3241 ATGCTCCCAC CATCCGTGCC CTCCAGGACC CCACTGAGGT GAACGTCGGG GGTTCTGTGG 
     3301 ACATAGTCTG CACTGTCGAT GCCAATCCCA TCCTCCCGGG CATGTTCAAC TGGGAGAGAC 
     3361 TGGGAGAAGA TGAGGAGGAC CAGAGCCTGG ATGACATGGA GAAGATATCC AGGGGACCAA 
     3421 CGGGGCGCCT GCGGATTCAC CATGCCAAAC TGGCCCAGGC TGGCGCTTAC CAGTGCATTG 
     3481 TGGACAATGG GGTGGCGCCT CCAGCACGAC GGCTGCTCCG TCTTGTTGTC AGATTTGCCC 
     3541 CCCAGGTGGA GCACCCCACT CCCCTAACTA AGGTGGCTGC AGCTGGAGAC AGCACCAGTT 
     3601 CTGCCACCCT CCACTGCCGT GCCCGAGGTG TCCCCAACAT CGTTTTCACT TGGACAAAAA 
     3661 ACGGGGTCCC TCTGGATCTC CAAGATCCCA GGTACACGGA GCACACATAC CACCAGGGTG 
     3721 GTGTCCACAG CAGCCTCCTG ACCATTGCCA ACGTGTCTGC CGCCCAGGAT TACGCCCTCT 
     3781 TCACATGTAC AGCCACCAAC GCCCTTGGCT CGGACCAAAC CAACATTCAA CTTGTCAGCA 
     3841 TCAGCCGCCC TGACCCTCCA TCAGGATTAA AGGTTGTGAG TCTGACCCCA CACTCCGTGG 
     3901 GGCTGGAGTG GAAGCCTGGC TTTGATGGGG GCCTGCCACA GAGGTTCTGC ATCAGGTATG 
     3961 AGGCCCTGGG GACTCCAGGG TTCCACTATG TGGATGTCGT ACCACCCCAG GCCACCACCT 
     4021 TCACGCTGAC TGGTCTACAG CCTTCTACAA GATACAGGGT CTGGCTGCTG GCCAGTAATG 
     4081 CCTTGGGGGA CAGTGGACTG GCTGACAAAG GGACCCAGCT TCCCATCACT ACCCCAGGTC 
     4141 TCCACCAGCC TTCTGGAGAA CCTGAAGACC AGCTGCCCAC AGAGCCACCT TCAGGACCCT 
     4201 CGGGGCTGCC CCTGCTGCCT GTGCTGTTCG CTCTTGGGGG GCTTCTGCTC CTCTCCAATG 
     4261 CCTCCTGTGT CGGGGGGGTC CTCTGGCAGC GGAGACTCAG GCGTCTTGCT GAGGGCATCT 
     4321 CAGAGAAGAC AGAGGCAGGG TCGGAAGAGG ACCGAGTCAG GAACGAATAT GAGGAGAGCC 
     4381 AGTGGACAGG AGAGCGGGAC ACTCAGAGCT CCACGGTCAG CACAACAGAG GCAGAGCCGT 
     4441 ATTACCGCTC CCTGAGGGAC TTCAGCCCCC AGCTGCCCCC GACGCAGGAG GAGGTGTCTT 
     4501 ATTCCCGAGG TTTCACAGGT GAAGATGAGG ATATGGCCTT CCCTGGGCAC TTGTATGATG 
     4561 AGGTAGAAAG AACGTACCCC CCGTCTGGAG CCTGGGGACC CCTCTACGAT GAAGTGCAGA 
     4621 TGGGACCCTG GGACCTCCAC TGGCCTGAAG ACACATATCA GGATCCAAGA GGAATCTATG 
     4681 ACCAGGTGGC CGGAGACTTG GACACTCTGG AACCCGATTC TCTGCCCTTC GAGCTGAGGG 
     4741 GACATCTGGT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4801 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4861 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4921 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4981 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5041 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5101 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5161 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5221 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5281 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5341 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5401 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5461 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5521 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5581 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5641 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5701 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5761 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5821 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5881 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5941 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6001 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6061 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6121 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6181 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6241 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6301 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6361 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6421 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6481 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6541 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6601 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6661 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6721 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6781 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6841 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6901 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6961 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7021 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7081 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7141 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7201 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7261 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7321 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7381 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7441 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7501 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7561 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7621 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7681 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7741 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7801 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7861 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7921 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7981 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8041 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8101 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8161 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8221 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8281 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8341 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8401 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8461 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8521 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8581 TAACAAAAAT TTAACGCGAA TTTT
//