LOCUS       RC217675                8115 bp    DNA     circular  SYN 
DEFINITION  DAAM1 (Myc-DDK-tagged)-Human dishevelled associated activator of morphogenesis 1 (DAAM1)
ACCESSION   NM_014992
VERSION     NM_014992.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4272..4363 
                     /label="Myc-DDK"  
     misc_feature    4393..4978 
                     /label="PolyA signal"  
     misc_feature    4450..4469 
                     /label="Restriction Enzyme"  
     misc_feature    5254..5926 
                     /label="ColE1"  
     misc_feature    6356..7150 
                     /label="Kan/Neo"  
     misc_feature    7185..7487 
                     /label="SV40 ori"  
     misc_feature    7573..8050 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4254..4260 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4254 
                     /label="DAAM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCAAGA AAGAGAGGTG GACGAGGTAT TTCATTCATC TTTTGCTGTT 
     1081 TCCGAAATAA TGATCACCCA GAAATCACGT ATCGGCTGCG AAATGATAGC AACTTTGCGC 
     1141 TTCAGACCAT GGAACCAGCA TTGCCCATGC CCCCTGTGGA GGAGCTGGAT GTCATGTTCA 
     1201 GTGAACTGGT GGATGAACTG GACCTCACAG ACAAACACAG AGAAGCCATG TTTGCACTTC 
     1261 CAGCTGAGAA AAAATGGCAA ATATACTGTA GCAAGAAAAA GGACCAGGAA GAAAACAAGG 
     1321 GAGCTACAAG TTGGCCTGAA TTCTACATTG ATCAGCTCAA TTCCATGGCT GCTAGAAAAT 
     1381 CTCTGCTGGC TTTAGAGAAG GAAGAAGAAG AAGAAAGAAG TAAAACTATA GAGAGTTTAA 
     1441 AGACAGCACT GAGGACAAAA CCAATGAGGT TTGTAACCAG ATTCATCGAC TTGGATGGCC 
     1501 TATCATGTAT CCTCAACTTT CTAAAGACCA TGGACTACGA GACCTCAGAG TCTCGAATAC 
     1561 ATACTTCTCT CATTGGCTGT ATAAAGGCGT TAATGAACAA CTCTCAAGGC CGGGCTCACG 
     1621 TCCTGGCTCA TTCTGAGAGT ATTAATGTAA TTGCTCAGAG TCTGAGCACA GAGAACATTA 
     1681 AAACGAAGGT GGCCGTGCTG GAAATCTTGG GCGCCGTGTG CCTGGTTCCC GGGGGCCACA 
     1741 AGAAGGTTCT GCAGGCCATG CTGCACTACC AGAAGTATGC CAGCGAAAGG ACCCGCTTTC 
     1801 AGACATTAAT TAACGACTTG GATAAAAGCA CTGGGCGGTA TCGAGATGAA GTGAGTCTCA 
     1861 AGACTGCCAT CATGTCCTTC ATTAATGCAG TGCTCAGCCA AGGTGCAGGA GTGGAGAGTT 
     1921 TGGACTTTAG ACTTCATCTT CGCTATGAAT TTCTGATGTT AGGAATTCAA CCTGTAATAG 
     1981 ATAAATTAAG GGAACACGAA AATTCAACAT TAGATAGGCA TTTAGACTTT TTTGAAATGC 
     2041 TCCGAAATGA AGATGAACTA GAATTTGCCA AAAGATTTGA ACTGGTTCAC ATAGACACAA 
     2101 AAAGTGCAAC TCAGATGTTT GAGCTGACCA GGAAGAGGCT GACACATAGT GAAGCTTACC 
     2161 CGCATTTCAT GTCCATCCTG CACCACTGCC TCCAAATGCC TTACAAGAGG AGTGGCAACA 
     2221 CTGTTCAGTA CTGGCTACTA CTAGATAGAA TTATACAGCA GATAGTTATC CAGAATGACA 
     2281 AAGGACAGGA CCCTGACTCC ACACCTTTGG AAAACTTTAA TATTAAGAAT GTCGTACGAA 
     2341 TGTTGGTTAA TGAAAATGAA GTTAAGCAGT GGAAAGAACA AGCGGAAAAA ATGAGAAAAG 
     2401 AGCACAATGA GCTACAGCAG AAACTGGAAA AGAAAGAACG AGAATGTGAT GCTAAGACTC 
     2461 AAGAGAAGGA AGAGATGATG CAGACCTTAA ATAAAATGAA AGAGAAACTT GAAAAGGAGA 
     2521 CTACTGAGCA TAAGCAAGTC AAGCAGCAGG TGGCGGACCT CACAGCACAG CTCCATGAGC 
     2581 TCAGCAGGAG GGCCGTCTGT GCTTCAATCC CAGGTGGACC CTCGCCTGGA GCACCAGGAG 
     2641 GGCCCTTTCC TTCCTCTGTG CCTGGATCTC TCCTTCCTCC CCCACCACCC CCACCTCTAC 
     2701 CAGGTGGGAT GCTTCCCCCT CCACCGCCTC CCCTCCCTCC AGGTGGCCCT CCTCCTCCCC 
     2761 CAGGGCCTCC TCCCTTAGGG GCAATCATGC CACCTCCTGG TGCTCCAATG GGCCTAGCAC 
     2821 TGAAGAAGAA AAGCATTCCT CAGCCCACAA ATGCCCTGAA ATCCTTCAAC TGGTCTAAAC 
     2881 TGCCCGAGAA CAAACTGGAA GGAACAGTAT GGACCGAAAT TGATGATACA AAAGTCTTCA 
     2941 AAATTCTAGA TCTTGAAGAC CTGGAAAGAA CCTTCTCTGC CTATCAAAGA CAGCAGGATT 
     3001 TCTTTGTGAA CAGTAACTCC AAGCAGAAAG AAGCAGATGC CATTGATGAC ACTCTGAGTT 
     3061 CCAAACTTAA AGTTAAAGAG CTTTCGGTGA TTGATGGTCG GAGAGCTCAG AATTGCAACA 
     3121 TCCTTCTATC GAGGTTGAAA TTATCCAATG ACGAAATCAA ACGGGCAATT CTAACAATGG 
     3181 ACGAACAGGA AGATCTGCCC AAGGACATGT TGGAACAGCT CTTGAAATTT GTTCCTGAAA 
     3241 AAAGTGACAT TGACCTATTG GAGGAACATA AACACGAACT GGATCGGATG GCCAAGGCTG 
     3301 ATAGGTTCCT TTTTGAGATG AGCCGAATTA ATCACTATCA GCAAAGGTTG CAATCGCTGT 
     3361 ACTTCAAAAA GAAGTTTGCA GAGCGTGTGG CAGAAGTGAA ACCTAAAGTG GAAGCAATTC 
     3421 GTTCTGGCTC AGAAGAGGTG TTTAGGAGTG GTGCCCTCAA GCAGTTGCTG GAGGTGGTTT 
     3481 TGGCATTTGG AAATTATATG AATAAAGGTC AAAGAGGGAA TGCATATGGA TTCAAGATAT 
     3541 CTAGCCTAAA CAAAATTGCT GACACAAAAT CCAGCATCGA CAAAAACATT ACCCTTTTGC 
     3601 ACTATCTCAT CACTATTGTG GAAAATAAGT ACCCCAGTGT TCTCAATCTA AATGAAGAAT 
     3661 TGCGAGATAT TCCTCAAGCT GCGAAAGTAA ACATGACTGA GCTGGACAAA GAAATAAGTA 
     3721 CCTTGAGAAG TGGCTTGAAA GCAGTAGAGA CAGAGCTGGA ATATCAGAAG TCTCAGCCCC 
     3781 CACAGCCCGG AGATAAGTTT GTGTCTGTTG TCAGCCAGTT CATCACAGTA GCCAGCTTCA 
     3841 GCTTCTCTGA TGTTGAAGAC CTTCTAGCAG AAGCTAAAGA CCTGTTTACT AAAGCAGTGA 
     3901 AGCACTTTGG GGAAGAGGCT GGCAAAATAC AACCAGATGA GTTCTTTGGC ATTTTTGATC 
     3961 AATTTCTTCA AGCTGTGTCA GAAGCCAAAC AAGAAAACGA AAATATGAGA AAGAAAAAGG 
     4021 AGGAAGAAGA ACGTCGAGCT CGCATGGAAG CTCAGCTCAA AGAACAACGT GAAAGGGAAC 
     4081 GTAAAATGAG AAAAGCTAAA GAGAATAGTG AAGAAAGCGG AGAGTTTGAT GACCTTGTTT 
     4141 CAGCTTTACG CTCAGGAGAA GTGTTTGACA AAGACCTTTC TAAATTGAAA CGGAATCGCA 
     4201 AACGTATTAC CAACCAGATG ACTGACAGCA GCAGAGAGAG ACCAATCACA AAACTTAATT 
     4261 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4321 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4381 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4441 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4501 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4561 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4621 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4681 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4741 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4801 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4861 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4921 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4981 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5041 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5101 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5161 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5221 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5281 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5341 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5401 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5461 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5521 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5581 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5641 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5701 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5761 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5821 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5881 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5941 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6001 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6061 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6121 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6181 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6241 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6301 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6361 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6421 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6481 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6541 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6601 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6661 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6721 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6781 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6841 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6901 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6961 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7021 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7081 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7141 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7201 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7261 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7321 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7381 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7441 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7501 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7561 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7621 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7681 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7741 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7801 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7861 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7921 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7981 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8041 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8101 TTTAACGCGA ATTTT
//