LOCUS       RC217598                9267 bp    DNA     circular  SYN 
DEFINITION  NKTR (Myc-DDK-tagged)-Human natural killer-tumor recognition sequence (NKTR)
ACCESSION   NM_005385
VERSION     NM_005385.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5424..5515 
                     /label="Myc-DDK"  
     misc_feature    5545..6130 
                     /label="PolyA signal"  
     misc_feature    5602..5621 
                     /label="Restriction Enzyme"  
     misc_feature    6406..7078 
                     /label="ColE1"  
     misc_feature    7508..8302 
                     /label="Kan/Neo"  
     misc_feature    8337..8639 
                     /label="SV40 ori"  
     misc_feature    8725..9202 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5406..5412 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5406 
                     /label="NKTR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGCGCAG GACCGGCCGC AGTGCCACTT CGACATCGAG ATCAACCGGG 
     1081 AGCCGGTTGG TCGCATTATG TTTCAGCTCT TCTCAGACAT ATGTCCAAAA ACATGCAAAA 
     1141 ACTTCCTTTG CTTGTGCTCA GGAGAGAAAG GCCTTGGGAA AACAACTGGG AAGAAGTTAT 
     1201 GTTATAAAGG TTCTACGTTC CATCGTGTGG TTAAAAACTT TATGATTCAG GGTGGGGACT 
     1261 TCAGTGAAGG TAATGGAAAA GGTGGAGAAT CAATTTATGG TGGATATTTT AAAGATGAAA 
     1321 ACTTTATTCT CAAACATGAC AGAGCGTTCC TTTTATCAAT GGCAAATCGA GGGAAACATA 
     1381 CCAATGGTTC CCAGTTTTTC ATTACCACAA AGCCTGCTCC ACACCTGGAT GGGGTGCATG 
     1441 TAGTCTTTGG ACTGGTTATT TCTGGTTTTG AAGTAATCGA ACAAATTGAA AATCTGAAGA 
     1501 CCGATGCTGC AAGCAGACCA TATGCAGATG TGCGAGTTAT TGACTGTGGA GTACTTGCCA 
     1561 CAAAATCAAT AAAAGATGTT TTTGAGAAAA AAAGGAAGAA ACCAACTCAT TCAGAAGGCT 
     1621 CGGATTCCTC TTCCAATTCC TCCTCTTCTT CAGAATCATC TTCAGAAAGT GAACTTGAAC 
     1681 ATGAGAGAAG CAGAAGGAGG AAACATAAGA GGAGGCCAAA AGTTAAACGT TCTAAAAAGA 
     1741 GGCGAAAGGA AGCAAGCAGT TCAGAAGAGC CAAGGAATAA ACATGCAATG AACCCAAAAG 
     1801 GTCACTCTGA GAGGAGTGAT ACCAATGAAA AAAGGTCAGT TGATTCCAGT GCTAAAAGGG 
     1861 AAAAACCTGT GGTCCGCCCA GAAGAGATTC CTCCAGTGCC TGAGAACCGA TTTTTACTGA 
     1921 GAAGAGATAT GCCTGTTGTT ACTGCAGAAC CTGAACCGAA GATTCCTGAT GTTGCACCCA 
     1981 TTGTAAGTGA TCAGAAACCA TCTGTATCAA AGTCTGGACG GAAGATTAAA GGAAGGGGCA 
     2041 CAATTCGCTA TCACACACCT CCAAGATCAA GATCCTGTTC TGAGTCAGAT GATGATGACA 
     2101 GCAGTGAAAC TCCTCCTCAC TGGAAAGAGG AAATGCAGAG ATTAAGAGCA TATAGACCAC 
     2161 CTAGTGGAGA AAAATGGAGT AAAGGAGATA AGTTAAGTGA CCCCTGTTCA AGCCGATGGG 
     2221 ATGAAAGAAG CTTGTCTCAG AGATCCAGAT CATGGTCCTA TAATGGATAT TATTCAGACC 
     2281 TTAGTACAGC AAGACACTCT GGCCACCATA AAAAACGCAG AAAAGAAAAA AAGGTTAAGC 
     2341 ATAAAAAGAA AGGGAAAAAG CAGAAACACT GCAGAAGACA CAAACAAACA AAGAAGAGAA 
     2401 GGATTCTTAT ACCGTCTGAC ATAGAATCCT CAAAATCTTC CACTCGAAGA ATGAAATCCT 
     2461 CTTGTGATAG AGAAAGGAGT TCTCGTTCTT CCTCATTGTC ATCTCATCAC TCATCAAAGA 
     2521 GAGACTGGTC TAAATCTGAT AAGGATGTCC AGAGCTCTTT AACCCATTCC AGCAGAGACT 
     2581 CATACAGATC AAAATCTCAC TCACAGTCTT ATTCTAGAGG AAGCTCAAGA TCAAGGACTG 
     2641 CGTCAAAGTC CTCATCACAT TCTCGAAGTA GATCAAAGTC CAGATCTAGT TCCAAGTCTG 
     2701 GGCACCGAAA GAGAGCATCA AAATCACCAA GAAAAACAGC TTCTCAGTTA AGTGAAAATA 
     2761 AACCAGTTAA AACAGAACCT TTAAGAGCAA CCATGGCACA AAATGAAAAT GTAGTAGTAC 
     2821 AACCAGTTGT AGCAGAAAAT ATTCCTGTAA TACCACTGAG TGACAGTCCC CCCCCTTCAA 
     2881 GATGGAAGCC TGGACAGAAA CCTTGGAAGC CCTCTTATGA GCGAATTCAG GAAATGAAAG 
     2941 CTAAAACAAC CCATTTGCTA CCCATCCAAA GCACTTACAG TTTAGCAAAT ATTAAAGAGA 
     3001 CTGGTAGCTC ATCATCCTAC CATAAAAGAG AAAAAAATTC GGAAAGTGAT CAGAGCACTT 
     3061 ATTCAAAATA CAGTGATAGA AGTTCAGAAA GCTCACCAAG GTCAAGGAGC AGATCTTCTA 
     3121 GGAGTAGATC TTATTCCAGA TCATATACAA GATCACGTAG TCTAGCTAGT TCACATTCAA 
     3181 GGTCTAGGTC TCCATCATCT AGATCTCATT CACGAAATAA ATACAGTGAT CATTCACAGT 
     3241 GTAGTAGATC ATCTTCATAT ACTTCTATTA GCAGTGATGA TGGAAGGCGA GCTAAGAGGA 
     3301 GACTTAGATC CAGTGGGAAA AAAAATAGCG TTTCACATAA AAAGCATAGC AGCAGCTCTG 
     3361 AAAAGACACT TCACAGTAAA TATGTCAAAG GTAGAGACAG GTCTTCATGT GTGAGAAAGT 
     3421 ATAGCGAGAG CAGATCATCT TTAGATTATT CTTCAGACAG TGAGCAGTCA AGTGTTCAGG 
     3481 CCACACAGTC AGCCCAGGAA AAAGAGAAGC AGGGCCAAAT GGAAAGAACA CATAATAAAC 
     3541 AAGAAAAAAA CAGAGGTGAA GAAAAATCCA AGTCTGAACG GGAATGCCCT CATTCAAAAA 
     3601 AAAGAACTTT GAAAGAGAAT CTTTCTGATC ACCTTAGAAA TGGCAGTAAG CCCAAAAGGA 
     3661 AGAATTATGC TGGTAGTAAA TGGGACTCTG AGTCAAATTC AGAACGAGAT GTCACTAAAA 
     3721 ACAGTAAAAA TGACTCCCAT CCATCCTCTG ACAAGGAAGA AGGTGAGGCC ACATCCGATT 
     3781 CTGAATCAGA GGTTAGTGAA ATTCACATCA AAGTCAAACC CACAACCAAG TCGTCCACAA 
     3841 ATACTTCACT GCCTGATGAT AATGGTGCTT GGAAATCAAG CAAACAGCGC ACATCAACTT 
     3901 CTGACTCTGA GGGGTCCTGT TCCAATTCGG AAAACAATAG GGGAAAGCCA CAAAAGCACA 
     3961 AACATGGGTC AAAGGAAAAT CTTAAAAGAG AACACACCAA AAAAGTGAAA GAGAAATTGA 
     4021 AAGGGAAAAA AGACAAAAAG CATAAGGCTC CAAAACGAAA GCAAGCATTT CACTGGCAGC 
     4081 CTCCACTAGA ATTTGGTGAA GAGGAGGAGG AGGAGATTGA TGACAAGCAA GTTACTCAGG 
     4141 AATCAAAAGA GAAAAAAGTT TCTGAAAACA ATGAAACCAT AAAAGATAAT ATTCTAAAAA 
     4201 CTGAGAAATC CAGTGAAGAG GACCTTTCAG GTAAACATGA TACAGTGACT GTTTCATCAG 
     4261 ATCTTGATCA GTTTACTAAA GATGATAGTA AACTCAGTAT TTCTCCCACA GCTTTAAATA 
     4321 CTGAGGAAAA TGTGGCCTGT TTACAAAACA TTCAGCACGT TGAAGAAAGT GTTCCCAATG 
     4381 GAGTGGAAGA TGTGCTTCAA ACAGATGACA ACATGGAGAT CTGCACTCCT GATAGGAGTT 
     4441 CCCCAGCAAA AGTAGAGGAG ACTTCCCCTC TAGGAAATGC ACGGCTTGAT ACCCCAGATA 
     4501 TAAACATTGT TTTGAAGCAG GATATGGCAA CGGAACATCC TCAAGCAGAG GTAGTAAAAC 
     4561 AGGAAAGCAG CATGTCCGAA AGTAAAGTGT TGGGTGAAGT GGGGAAACAG GACAGCAGCT 
     4621 CTGCTAGCTT GGCTAGTGCT GGAGAAAGTA CCGGGAAGAA GGAGGTGGCT GAGAAGAGCC 
     4681 AGATCAACCT CATTGATAAG AAATGGAAGC CCCTGCAAGG TGTGGGGAAC CTGGCAGCAC 
     4741 CTAATGCTGC CACATCCAGT GCTGTGGAAG TTAAGGTGTT GACCACTGTG CCTGAAATGA 
     4801 AACCACAAGG CTTGAGAATA GAAATTAAAA GCAAAAATAA AGTTCGGCCT GGGTCTCTCT 
     4861 TTGATGAAGT AAGAAAGACA GCACGCTTAA ACCGTAGACC AAGAAATCAG GAGAGTTCAA 
     4921 GTGATGAGCA GACGCCTAGT CGGGATGATG ATAGCCAGTC CAGGAGTCCA AGTAGATCTC 
     4981 GAAGTAAATC TGAAACCAAA TCAAGACACA GAACAAGGTC TGTCTCCTAT AGTCACTCAA 
     5041 GAAGTCGATC GAGAAGTTCC ACATCATCTT ATCGATCAAG AAGCTACTCT AGAAGTCGGA 
     5101 GCAGAGGATG GTACAGCAGA GGCCGAACCA GAAGCCGGAG CAGTTCCTAC CGGAGTTACA 
     5161 AAAGTCACAG GACGTCCAGC AGGAGCAGAT CCAGGAGCAG CTCATATGAT CCCCACAGTC 
     5221 GATCCAGGTC CTACACCTAC GATAGCTACT ATAGCAGGAG TCGGAGTCGA AGTAGAAGCC 
     5281 AGAGAAGTGA CAGTTACCAC CGAGGCAGAA GTTATAATCG GCGGTCCAGG AGTTGTAGAT 
     5341 CTTATGGCTC TGACAGTGAA AGTGACCGAA GTTACTCTCA TCACCGGAGC CCCAGTGAGA 
     5401 GCAGCAGATA CAGTACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5461 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5521 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5581 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5641 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5701 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5761 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5821 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5881 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5941 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6001 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6061 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6121 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6181 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6241 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6301 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6361 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6421 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6481 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6541 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6601 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6661 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6721 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6781 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6841 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6901 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6961 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7021 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7081 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7141 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7201 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7261 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7321 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7381 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7441 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7501 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7561 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7621 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7681 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7741 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7801 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7861 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7921 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7981 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8041 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8101 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8161 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8221 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8281 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8341 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8401 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8461 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8521 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8581 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8641 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8701 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8761 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8821 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8881 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8941 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9001 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9061 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9121 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9181 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9241 ATTTAACAAA AATTTAACGC GAATTTT
//