LOCUS       RC217567                6738 bp    DNA     circular  SYN 
DEFINITION  KLC4 (Myc-DDK-tagged)-Human kinesin light chain 4 (KLC4), transcript variant 2
ACCESSION   NM_201522
VERSION     NM_201522.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2895..2986 
                     /label="Myc-DDK"  
     misc_feature    3016..3601 
                     /label="PolyA signal"  
     misc_feature    3073..3092 
                     /label="Restriction Enzyme"  
     misc_feature    3877..4549 
                     /label="ColE1"  
     misc_feature    4979..5773 
                     /label="Kan/Neo"  
     misc_feature    5808..6110 
                     /label="SV40 ori"  
     misc_feature    6196..6673 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2877..2883 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2877 
                     /label="KLC4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGGCCTG GTGTTGGGGC AGCGGGATGA GCCTGCAGGC CACCGGCTCA 
     1081 GCCAAGAGGA GATCCTGGGG AGCACACGGC TGGTCAGCCA AGGGCTAGAG GCCCTACGCA 
     1141 GTGAACACCA GGCCGTGCTG CAAAGCCTGT CCCAGACCAT TGAGTGTCTG CAGCAGGGAG 
     1201 GCCATGAGGA AGGGCTGGTG CATGAGAAGG CCCGGCAGCT TCGCCGTTCT ATGGAAAACA 
     1261 TTGAGCTCGG GCTGAGTGAG GCCCAGGTGA TGCTGGCTCT AGCCAGCCAC CTGAGCACAG 
     1321 TGGAGTCGGA GAAACAGAAG CTGCGGGCTC AGGTGCGGCG GCTATGCCAG GAGAACCAGT 
     1381 GGCTGCGGGA TGAGCTGGCT GGCACCCAGC AGCGGCTACA GCGCAGTGAA CAGGCTGTGG 
     1441 CTCAGCTGGA GGAGGAAAAG AAGCACCTGG AGTTCCTGGG GCAGCTGCGG CAGTATGATG 
     1501 AGGATGGACA TACCTCGGAG GAGAAAGAAG GCGATGCCAC CAAGGATTCC CTGGATGACC 
     1561 TCTTTCCTAA TGAGGAGGAA GAGGACCCCA GCAATGGCTT GTCCCGTGGT CAAGGTGCTA 
     1621 CAGCAGCTCA GCAGGGTGGA TATGAGATCC CAGCAAGGTT GCGGACGTTG CACAACCTGG 
     1681 TGATCCAGTA CGCAGCCCAA GGTCGCTATG AGGTGGCCGT GCCACTCTGT AAGCAGGCAC 
     1741 TAGAGGACCT GGAGCGCACA TCAGGCCGTG GCCACCCTGA TGTCGCCACC ATGCTCAACA 
     1801 TCCTTGCTTT GGTGTATCGT GACCAGAATA AGTATAAGGA AGCTGCCCAC CTGCTGAATG 
     1861 ATGCCCTTAG CATCCGGGAG AGCACCTTGG GACCTGACCA TCCTGCTGTG GCTGCCACAC 
     1921 TCAACAATTT GGCTGTGCTC TATGGCAAAA GGGGCAAGTA CAAGGAGGCA GAGCCTCTGT 
     1981 GCCAGCGGGC ACTGGAGATT CGAGAAAAGG TCCTGGGCAC GAATCATCCA GATGTGGCAA 
     2041 AACAGCTGAA CAACCTGGCC CTCTTGTGCC AAAACCAGGG CAAGTATGAG GCCGTGGAAC 
     2101 GCTACTACCA GCGAGCACTG GCCATCTACG AGGGGCAGCT GGGGCCGGAC AACCCTAATG 
     2161 TAGCCCGGAC CAAGAACAAC CTGGCTTCCT GTTACCTGAA ACAGGGCAAA TATGCTGAGG 
     2221 CTGAGACACT ATACAAAGAG ATCCTGACCC GTGCCCATGT ACAGGAGTTT GGGTCTGTGG 
     2281 ATGATGACCA CAAGCCCATC TGGATGCATG CAGAGGAGCG GGAGGAAATG AGCAAAAGCC 
     2341 GGCACCATGA GGGTGGGACA CCCTATGCTG AGTATGGAGG CTGGTACAAG GCCTGCAAAG 
     2401 TGAGCAGCCC CACAGTGAAC ACTACTCTGA GAAACCTGGG AGCTCTGTAT AGGCGCCAGG 
     2461 GAAAGCTGGA GGCTGCTGAG ACCCTGGAGG AATGTGCCCT GCGGTCCCGG AGACAGGGCA 
     2521 CTGACCCTAT CAGCCAGACG AAGGTGGCAG AGCTGCTTGG GGAGAGTGAT GGTAGAAGGA 
     2581 CCTCCCAGGA GGGCCCTGGA GACAGTGTGA AATTCGAGGG AGGTGAAGAT GCTTCTGTGG 
     2641 CTGTGGAGTG GTCCGGGGAT GGCAGTGGGA CCCTGCAGAG GAGTGGCTCT CTTGGCAAGA 
     2701 TCCGGGATGT GCTCCGCAGA AGCAGTGAAC TCTTGGTGAG GAAGCTCCAG GGGACTGAGC 
     2761 CTCGGCCCTC CAGCAGCAAC ATGAAGCGAG CAGCCTCCTT GAACTATCTG AACCAACCTA 
     2821 GTGCAGCACC CCTCCAGGTC TCCCGGGGCC TCAGTGCCAG CACCATGGAC CTCTCTTCAA 
     2881 GCAGCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     2941 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3001 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3061 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3121 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3181 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3241 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3301 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3361 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3421 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3481 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3541 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     3601 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     3661 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     3721 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     3781 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     3841 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     3901 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     3961 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4021 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4081 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4141 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4201 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4261 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4321 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4381 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4441 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4501 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4561 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     4621 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     4681 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     4741 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     4801 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     4861 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     4921 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     4981 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5041 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5101 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5161 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5221 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5281 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5341 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5401 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5461 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5521 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     5581 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     5641 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     5701 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     5761 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     5821 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     5881 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     5941 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6001 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6061 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6121 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6181 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6241 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6301 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6361 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6421 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6481 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6541 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     6601 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     6661 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     6721 AAATTTAACG CGAATTTT
//