LOCUS       RC217532                7233 bp    DNA     circular  SYN 
DEFINITION  SP4 (Myc-DDK-tagged)-Human Sp4 transcription factor (SP4)
ACCESSION   NM_003112
VERSION     NM_003112.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3390..3481 
                     /label="Myc-DDK"  
     misc_feature    3511..4096 
                     /label="PolyA signal"  
     misc_feature    3568..3587 
                     /label="Restriction Enzyme"  
     misc_feature    4372..5044 
                     /label="ColE1"  
     misc_feature    5474..6268 
                     /label="Kan/Neo"  
     misc_feature    6303..6605 
                     /label="SV40 ori"  
     misc_feature    6691..7168 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3372..3378 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3372 
                     /label="SP4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGATCAG AAGAAGGAGG AGGAGGAGGA GGCGGCAGCG GCAGCGGCGA 
     1081 TGGCTACAGA AGGAGGGAAA ACCTCTGAGC CAGAGAATAA CAATAAAAAA CCCAAAACCT 
     1141 CAGGCTCCCA GGACTCTCAG CCCTCTCCTC TGGCTTTACT GGCAGCTACT TGCAGCAAAA 
     1201 TAGGGACTCC TGGTGAAAAT CAAGCAACTG GACAACAACA AATTATTATA GATCCAAGTC 
     1261 AAGGATTGGT GCAACTTCAA AATCAACCAC AACAGCTAGA ACTGGTAACA ACGCAACTTG 
     1321 CTGGAAACGC TTGGCAACTT GTTGCCTCCA CTCCTCCTGC TTCAAAAGAG AATAACGTTT 
     1381 CTCAACCAGC CTCTAGTTCG TCTAGTTCTT CCAGCAGTAA TAACGGGAGT GCATCTCCTA 
     1441 CAAAAACTAA ATCAGGTAAT TCTTCCACCC CTGGTCAATT TCAAGTCATA CAAGTACAAA 
     1501 ATCCAAGTGG TAGTGTACAG TACCAAGTAA TTCCACAACT TCAGACAGTG GAAGGTCAAC 
     1561 AAATTCAAAT CAATCCAACT AGTAGTTCAT CTCTACAGGA TTTGCAGGGT CAAATTCAGC 
     1621 TCATTTCTGC AGGTAATAAT CAAGCTATAC TCACAGCTGC TAACAGGACA GCTTCTGGGA 
     1681 ATATTCTTGC TCAAAACCTG GCAAATCAGA CAGTTCCGGT CCAAATTAGA CCTGGTGTTT 
     1741 CAATACCACT GCAGTTACAG ACTCTTCCTG GTACTCAGGC TCAAGTTGTA ACAACCCTAC 
     1801 CAATTAACAT TGGAGGAGTG ACTCTAGCTT TGCCAGTGAT AAACAACGTG GCTGCCGGAG 
     1861 GAGGGACTGG GCAGGTTGGC CAGCCTGCTG CTACTGCTGA TAGTGGGACT TCCAATGGGA 
     1921 ATCAATTAGT TTCCACACCC ACCAACACCA CTACTTCTGC CAGTACTATG CCAGAATCTC 
     1981 CCTCCTCCTC CACTACCTGC ACAACCACTG CTTCAACGTC TTTGACAAGC AGTGACACAT 
     2041 TAGTGAGCTC AGCAGATACT GGCCAGTATG CAAGCACATC AGCCAGTAGT TCTGAACGCA 
     2101 CCATTGAAGA ATCTCAAACA CCTGCTGCTA CTGAGTCTGA AGCCCAGAGC TCCAGTCAGC 
     2161 TTCAGCCTAA TGGAATGCAG AATGCACAGG ATCAATCAAA TTCTCTTCAG CAGGTGCAAA 
     2221 TTGTAGGCCA ACCTATCTTA CAGCAGATCC AGATCCAACA GCCTCAGCAA CAGATCATTC 
     2281 AGGCTATTCC ACCACAGTCG TTTCAACTCC AGTCAGGGCA GACGATTCAG ACCATCCAGC 
     2341 AGCAGCCTTT ACAGAATGTT CAACTTCAAG CAGTAAATCC GACTCAGGTG CTTATCAGGG 
     2401 CTCCAACTTT AACACCTTCA GGGCAAATCA GTTGGCAAAC TGTACAGGTT CAGAATATTC 
     2461 AGAGTCTTTC AAATTTGCAA GTTCAGAATG CTGGGTTATC CCAACAATTA ACCATCACCC 
     2521 CAGTGTCTTC AAGTGGTGGC ACAACTCTTG CTCAGATTGC TCCTGTGGCT GTTGCTGGTG 
     2581 CCCCAATAAC TTTGAATACT GCCCAGCTTG CATCAGTGCC TAACCTTCAG ACAGTGAGCG 
     2641 TTGCCAACCT GGGTGCTGCA GGTGTTCAAG TGCAGGGAGT TCCCGTTACA ATCACTAGTG 
     2701 TTGCAGGTCA GCAGCAAGGA CAAGATGGAG TAAAAGTCCA GCAAGCTACT ATAGCTCCTG 
     2761 TAACTGTAGC AGTTGGAGGA ATTGCTAATG CCACGATAGG TGCTGTTAGT CCTGACCAAC 
     2821 TCACACAAGT GCATTTGCAG CAAGGCCAGC AGACTTCTGA TCAAGAGGTA CAACCTGGCA 
     2881 AGAGGCTTCG AAGAGTTGCC TGTTCCTGTC CTAATTGTAG GGAAGGAGAA GGAAGAGGCA 
     2941 GTAATGAACC AGGAAAAAAG AAGCAGCATA TCTGTCATAT TGAAGGATGT GGTAAAGTTT 
     3001 ATGGCAAAAC ATCTCATTTA CGAGCACATC TTCGCTGGCA TACTGGAGAA AGACCTTTTA 
     3061 TATGCAACTG GATGTTTTGT GGCAAAAGAT TCACACGGAG TGATGAGCTC CAGAGACATA 
     3121 GAAGAACCCA TACAGGTGAA AAGAGATTTG AATGCCCGGA ATGTTCTAAA AGGTTTATGC 
     3181 GGAGTGATCA TCTCTCCAAA CATGTCAAAA CGCACCAGAA TAAAAAAGGT GGTGGGACAG 
     3241 CTCTTGCCAT TGTTACCTCG GGAGAACTGG ACTCATCTGT TACAGAGGTG CTTGGCTCCC 
     3301 CAAGAATTGT CACAGTTGCA GCCATTTCTC AAGATTCGAA TCCAGCAACT CCCAATGTTT 
     3361 CAACCAACAT GGAAGAATTC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3421 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3481 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3541 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3601 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3661 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3721 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3781 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3841 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3901 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3961 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4021 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4081 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4141 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4201 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4261 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4321 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4381 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4441 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4501 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4561 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4621 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4681 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4741 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4801 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4861 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4921 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4981 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5041 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5101 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5161 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5221 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5281 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5341 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5401 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5461 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5521 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5581 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5641 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5701 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5761 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5821 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5881 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5941 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6001 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6061 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6121 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6181 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6241 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6301 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6361 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6421 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6481 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6541 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6601 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6661 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6721 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6781 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6841 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6901 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6961 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7021 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7081 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7141 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7201 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//