LOCUS       RC217522                7017 bp    DNA     circular  SYN 
DEFINITION  TICAM1 (Myc-DDK-tagged)-Human toll-like receptor adaptor molecule 1 (TICAM1)
ACCESSION   NM_182919
VERSION     NM_182919.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3174..3265 
                     /label="Myc-DDK"  
     misc_feature    3295..3880 
                     /label="PolyA signal"  
     misc_feature    3352..3371 
                     /label="Restriction Enzyme"  
     misc_feature    4156..4828 
                     /label="ColE1"  
     misc_feature    5258..6052 
                     /label="Kan/Neo"  
     misc_feature    6087..6389 
                     /label="SV40 ori"  
     misc_feature    6475..6952 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3156..3162 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3156 
                     /label="TICAM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTGCACA GGCCCATCAC TTCCTAGCGC CTTCGACATT CTAGGTGCAG 
     1081 CAGGCCAGGA CAAGCTCTTG TATCTGAAGC ACAAACTGAA GACCCCACGC CCAGGCTGCC 
     1141 AGGGGCAGGA CCTCCTGCAT GCCATGGTTC TCCTGAAGCT GGGCCAGGAA ACTGAGGCCA 
     1201 GGATCTCTCT AGAGGCATTG AAGGCCGATG CGGTGGCCCG GCTGGTGGCC CGCCAGTGGG 
     1261 CTGGCGTGGA CAGCACCGAG GACCCAGAGG AGCCCCCAGA TGTGTCCTGG GCTGTGGCCC 
     1321 GCTTGTACCA CCTGCTGGCT GAGGAGAAGC TGTGCCCCGC CTCGCTGCGG GACGTGGCCT 
     1381 ACCAGGAAGC CGTCCGCACC CTCAGCTCCA GGGACGACCA CCGGCTGGGG GAACTTCAGG 
     1441 ATGAGGCCCG AAACCGGTGT GGGTGGGACA TTGCTGGGGA TCCAGGGAGC ATCCGGACGC 
     1501 TCCAGTCCAA TCTGGGCTGC CTCCCACCAT CCTCGGCTTT GCCCTCTGGG ACCAGGAGCC 
     1561 TCCCACGCCC CATTGACGGT GTTTCGGACT GGAGCCAAGG GTGCTCCCTG CGATCCACTG 
     1621 GCAGCCCTGC CTCCCTGGCC AGCAACTTGG AAATCAGCCA GTCCCCTACC ATGCCCTTCC 
     1681 TCAGCCTGCA CCGCAGCCCA CATGGGCCCA GCAAGCTCTG TGACGACCCC CAGGCCAGCT 
     1741 TGGTGCCCGA GCCTGTCCCC GGTGGCTGCC AGGAGCCTGA GGAGATGAGC TGGCCGCCAT 
     1801 CGGGGGAGAT TGCCAGCCCA CCAGAGCTGC CAAGCAGCCC ACCTCCTGGG CTTCCCGAAG 
     1861 TGGCCCCAGA TGCAACCTCC ACTGGCCTCC CTGATACCCC CGCAGCTCCA GAAACCAGCA 
     1921 CCAACTACCC AGTGGAGTGC ACCGAGGGGT CTGCAGGCCC CCAGTCTCTC CCCTTGCCTA 
     1981 TTCTGGAGCC GGTCAAAAAC CCCTGCTCTG TCAAAGACCA GACGCCACTC CAACTTTCTG 
     2041 TAGAAGATAC CACCTCTCCA AATACCAAGC CGTGCCCACC TACTCCCACC ACCCCAGAAA 
     2101 CATCCCCTCC TCCTCCTCCT CCTCCTCCTT CATCTACTCC TTGTTCAGCT CACCTGACCC 
     2161 CCTCCTCCCT GTTCCCTTCC TCCCTGGAAT CATCATCGGA ACAGAAATTC TATAACTTTG 
     2221 TGATCCTCCA CGCCAGGGCA GACGAACACA TCGCCCTGCG GGTTCGGGAG AAGCTGGAGG 
     2281 CCCTTGGCGT GCCCGACGGG GCCACCTTCT GCGAGGATTT CCAGGTGCCG GGGCGCGGGG 
     2341 AGCTGAGCTG CCTGCAGGAC GCCATAGACC ACTCAGCTTT CATCATCCTA CTTCTCACCT 
     2401 CCAACTTCGA CTGTCGCCTG AGCCTGCACC AGGTGAACCA AGCCATGATG AGCAACCTCA 
     2461 CGCGACAGGG GTCGCCAGAC TGTGTCATCC CCTTCCTGCC CCTGGAGAGC TCCCCGGCCC 
     2521 AGCTCAGCTC CGACACGGCC AGCCTGCTCT CCGGGCTGGT GCGGCTGGAC GAACACTCCC 
     2581 AGATCTTCGC CAGGAAGGTG GCCAACACCT TCAAGCCCCA CAGGCTTCAG GCCCGAAAGG 
     2641 CCATGTGGAG GAAGGAACAG GACACCCGAG CCCTGCGGGA ACAGAGCCAA CACCTGGATG 
     2701 GTGAGCGGAT GCAGGCGGCG GCACTGAACG CAGCCTACTC AGCCTACCTC CAGAGCTACT 
     2761 TGTCCTACCA GGCACAGATG GAGCAGCTCC AGGTGGCTTT TGGGAGCCAC ATGTCATTTG 
     2821 GGACTGGGGC GCCCTATGGG GCTCGAATGC CCTTTGGGGG CCAGGTGCCC CTGGGAGCCC 
     2881 CGCCACCCTT TCCCACTTGG CCGGGGTGCC CGCAGCCGCC ACCCCTGCAC GCATGGCAGG 
     2941 CTGGCACCCC CCCACCGCCC TCCCCACAGC CAGCAGCCTT TCCACAGTCA CTGCCCTTCC 
     3001 CGCAGTCCCC AGCCTTCCCT ACGGCCTCAC CCGCACCCCC TCAGAGCCCA GGGCTGCAAC 
     3061 CCCTCATTAT CCACCACGCA CAGATGGTAC AGCTGGGGCT GAACAACCAC ATGTGGAACC 
     3121 AGAGAGGGTC CCAGGCGCCC GAGGACAAGA CGCAGGAGGC AGAAACGCGT ACGCGGCCGC 
     3181 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3241 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3301 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3361 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3421 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3481 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3541 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3601 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3661 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3721 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3781 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3841 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     3901 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     3961 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4021 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4081 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4141 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4201 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4261 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4321 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4381 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4441 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4501 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4561 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4621 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4681 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4741 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4801 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4861 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     4921 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     4981 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5041 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5101 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5161 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5221 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5281 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5341 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5401 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5461 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5521 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5581 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5641 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5701 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5761 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5821 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     5881 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     5941 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6001 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6061 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6121 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6181 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6241 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6301 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6361 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6421 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6481 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6541 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6601 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6661 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6721 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6781 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6841 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     6901 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     6961 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//