LOCUS       RC217463                8814 bp    DNA     circular  SYN 
DEFINITION  ZNF521 (Myc-DDK-tagged)-Human zinc finger protein 521 (ZNF521)
ACCESSION   NM_015461
VERSION     NM_015461.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4971..5062 
                     /label="Myc-DDK"  
     misc_feature    5092..5677 
                     /label="PolyA signal"  
     misc_feature    5149..5168 
                     /label="Restriction Enzyme"  
     misc_feature    5953..6625 
                     /label="ColE1"  
     misc_feature    7055..7849 
                     /label="Kan/Neo"  
     misc_feature    7884..8186 
                     /label="SV40 ori"  
     misc_feature    8272..8749 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4953..4959 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4953 
                     /label="ZNF521"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCGCCGC AAGCAAGCGA AACCGAGATC CCTCAAAGAC CCCAACTGTA 
     1081 AACTTGAAGA CAAGACTGAA GATGGAGAGG CACTAGATTG TAAGAAGAGG CCGGAAGACG 
     1141 GGGAGGAGTT GGAAGACGAA GCTGTGCACA GCTGTGACAG CTGCCTCCAG GTGTTTGAAT 
     1201 CGCTGAGCGA TATCACAGAA CACAAGATTA ATCAATGTCA ACTGACAGAT GGAGTGGATG 
     1261 TTGAAGATGA TCCGACTTGC TCTTGGCCAG CTTCCTCACC TTCTAGCAAG GATCAGACTT 
     1321 CCCCTAGCCA TGGAGAAGGT TGCGATTTTG GAGAGGAAGA AGGTGGCCCT GGGCTTCCAT 
     1381 ACCCGTGTCA ATTCTGTGAC AAGTCGTTTA GCCGCCTCAG CTACCTAAAG CACCATGAGC 
     1441 AGAGTCACAG TGACAAACTG CCTTTCAAAT GCACCTACTG CAGTAGGCTG TTCAAACACA 
     1501 AGCGCAGCCG AGATCGCCAC ATAAAACTCC ACACCGGGGA CAAGAAGTAC CACTGCAGTG 
     1561 AATGTGATGC TGCGTTTTCC AGAAGTGATC ACTTGAAGAT CCACTTAAAG ACTCACACGT 
     1621 CCAACAAGCC ATATAAATGT GCCATTTGTC GCCGTGGGTT TCTGTCCTCT AGTTCCTTAC 
     1681 ACGGACACAT GCAGGTTCAT GAGAGGAACA AGGACGGCTC TCAGTCCGGT TCCAGGATGG 
     1741 AGGACTGGAA GATGAAGGAC ACTCAGAAGT GCAGTCAGTG TGAGGAAGGC TTTGACTTCC 
     1801 CGGAAGACCT CCAAAAACAC ATTGCAGAGT GCCACCCCGA ATGCTCCCCA AATGAGGACC 
     1861 GAGCGGCCCT CCAGTGTGTC TACTGCCACG AGCTCTTCGT AGAGGAGACC TCCCTCATGA 
     1921 ACCACATGGA GCAGGTGCAT AGCGGGGAGA AGAAGAACTC ATGCAGCATT TGTTCTGAGA 
     1981 GTTTCCACAC AGTTGAGGAA CTGTACAGCC ACATGGACAG TCACCAGCAA CCGGAGTCAT 
     2041 GCAATCACAG CAACAGCCCT TCCCTGGTCA CGGTGGGCTA TACCTCCGTG TCCAGTACGA 
     2101 CTCCAGATTC CAACCTCTCA GTGGACAGCT CAACCATGGT GGAAGCTGCC CCGCCAATCC 
     2161 CAAAGAGTCG AGGGAGGAAG AGGGCCGCTC AACAAACCCC TGACATGACT GGTCCCTCGA 
     2221 GTAAACAAGC AAAAGTTACC TACAGCTGTA TTTACTGCAA CAAACAATTA TTTTCAAGTC 
     2281 TTGCAGTTCT GCAGATTCAC CTGAAAACTA TGCACTTAGA TAAGCCAGAA CAGGCCCATA 
     2341 TTTGTCAGTA TTGCTTGGAG GTCCTGCCCT CACTCTATAA CCTAAATGAA CATCTTAAGC 
     2401 AAGTGCATGA AGCTCAGGAC CCAGGTCTGA TTGTTTCTGC CATGCCTGCC ATTGTCTACC 
     2461 AGTGTAACTT CTGTTCCGAA GTTGTCAACG ACCTCAACAC TCTTCAGGAA CACATCCGAT 
     2521 GTTCTCATGG ATTTGCAAAC CCTGCAGCTA AAGATAGTAA TGCATTCTTT TGTCCCCATT 
     2581 GCTATATGGG GTTTCTCACT GACTCTTCCC TGGAAGAGCA TATTAGACAG GTTCATTGTG 
     2641 ACCTCAGTGG CTCCCGATTT GGGTCTCCAG TGCTTGGGAC TCCCAAAGAA CCAGTAGTAG 
     2701 AAGTCTATTC TTGTTCCTAT TGTACAAATT CGCCAATATT CAACAGCGTT CTTAAACTGA 
     2761 ACAAGCATAT CAAAGAGAAT CATAAAAACA TTCCCTTGGC CCTGAATTAT ATCCACAATG 
     2821 GGAAGAAATC CAGGGCCTTA AGCCCCCTAT CTCCTGTGGC CATAGAGCAG ACATCTCTTA 
     2881 AGATGATGCA GGCAGTAGGA GGTGCACCTG CACGTCCCAC TGGAGAATAT ATCTGTAATC 
     2941 AATGTGGTGC TAAGTACACA TCCCTAGACA GCTTTCAGAC TCACCTAAAA ACTCATCTCG 
     3001 ACACTGTGCT TCCAAAATTG ACCTGTCCTC AGTGCAACAA GGAATTCCCC AACCAAGAAT 
     3061 CCTTGCTGAA GCATGTTACC ATTCACTTTA TGATCACTTC AACGTATTAC ATCTGTGAGA 
     3121 GTTGTGACAA GCAATTCACA TCAGTGGATG ACCTTCAGAA ACACCTGCTG GACATGCACA 
     3181 CCTTTGTCTT CTTTCGCTGC ACCCTCTGCC AGGAAGTTTT TGACTCAAAA GTCTCCATTC 
     3241 AGCTCCACTT GGCTGTGAAG CACAGTAACG AAAAGAAAGT CTATAGGTGC ACATCTTGCA 
     3301 ACTGGGACTT CCGCAACGAA ACTGACTTGC AGCTCCATGT GAAACACAAC CACCTGGAAA 
     3361 ACCAAGGGAA AGTGCATAAG TGCATTTTCT GCGGTGAGTC CTTTGGCACC GAGGTGGAGC 
     3421 TGCAATGCCA CATCACCACT CACAGTAAGA AGTACAACTG CAAGTTCTGT AGCAAAGCCT 
     3481 TCCATGCGAT CATTTTGTTA GAAAAACACT TGCGAGAAAA ACACTGTGTA TTCGAAACCA 
     3541 AGACACCCAA CTGTGGAACA AATGGAGCTT CCGAGCAAGT GCAGAAAGAG GAAGTGGAGC 
     3601 TGCAGACTTT GCTGACCAAC AGCCAGGAGT CCCACAACAG TCACGATGGG AGCGAAGAAG 
     3661 ACGTTGACAC CTCTGAGCCT ATGTACGGCT GCGACATTTG TGGGGCAGCC TACACTATGG 
     3721 AAACTTTGCT GCAGAATCAC CAGCTCCGAG ACCACAACAT CAGACCTGGA GAAAGTGCCA 
     3781 TCGTGAAAAA GAAAGCTGAG CTCATTAAAG GGAATTACAA GTGCAACGTG TGCTCTCGAA 
     3841 CCTTCTTCTC CGAAAATGGC CTCCGGGAAC ATATGCAGAC CCACCTAGGC CCTGTCAAAC 
     3901 ACTACATGTG CCCTATTTGC GGAGAGCGGT TTCCCTCCCT TTTAACTCTT ACTGAACACA 
     3961 AAGTCACGCA TAGTAAGAGT CTTGATACTG GAAACTGCCG GATTTGCAAG ATGCCTCTCC 
     4021 AGAGTGAAGA GGAGTTTTTA GAGCATTGCC AAATGCACCC TGACTTGAGG AATTCCCTGA 
     4081 CAGGCTTTCG CTGCGTGGTG TGCATGCAGA CAGTGACCTC CACCTTGGAA CTCAAAATCC 
     4141 ATGGGACGTT CCACATGCAA AAGACAGGGA ATGGGTCTGC AGTTCAGACC ACAGGGCGGG 
     4201 GCCAGCACGT CCAAAAACTG TATAAGTGCG CATCTTGCCT CAAAGAATTC CGTTCCAAGC 
     4261 AAGATCTGGT GAAACTTGAT ATCAATGGCC TGCCATATGG TCTGTGTGCC GGCTGCGTGA 
     4321 ATCTCAGTAA GAGCGCCAGC CCAGGCATTA ACGTCCCTCC CGGCACGAAT AGACCAGGCT 
     4381 TGGGCCAGAA TGAGAATCTG AGTGCCATTG AGGGGAAAGG CAAGGTGGGG GGACTGAAGA 
     4441 CACGCTGCTC TAGCTGCAAC GTTAAGTTTG AGTCTGAAAG TGAACTCCAG AACCACATCC 
     4501 AAACCATCCA CCGAGAGCTC GTGCCAGACA GCAACAGCAC ACAGTTGAAA ACGCCCCAAG 
     4561 TATCACCAAT GCCCAGAATC AGTCCCTCCC AGTCGGATGA GAAGAAGACC TATCAATGCA 
     4621 TCAAGTGTCA GATGGTTTTC TACAATGAAT GGGATATTCA GGTTCATGTT GCAAATCACA 
     4681 TGATTGATGA AGGACTGAAC CATGAATGCA AACTCTGCAG CCAGACCTTT GACTCTCCTG 
     4741 CCAAACTCCA GTGCCACCTG ATAGAGCACA GCTTCGAAGG GATGGGAGGC ACCTTCAAGT 
     4801 GTCCAGTCTG CTTTACAGTA TTTGTTCAAG CAAACAAGTT GCAGCAGCAT ATTTTCTCTG 
     4861 CCCATGGACA AGAAGACAAG ATCTATGACT GTACACAATG TCCACAGAAG TTTTTCTTCC 
     4921 AAACAGAGCT GCAGAATCAT ACAATGACCC AACACAGCAG TACGCGTACG CGGCCGCTCG 
     4981 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5041 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5101 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5161 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5221 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5281 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5341 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5401 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5461 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5521 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5581 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5641 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5701 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5761 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5821 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5881 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5941 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6001 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6061 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6121 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6181 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6241 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6301 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6361 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6421 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6481 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6541 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6601 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6661 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6721 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6781 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6841 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6901 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6961 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7021 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7081 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7141 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7201 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7261 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7321 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7381 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7441 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7501 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7561 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7621 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7681 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7741 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7801 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7861 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7921 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7981 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8041 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8101 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8161 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8221 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8281 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8341 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8401 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8461 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8521 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8581 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8641 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8701 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8761 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//