LOCUS       RC217395                8319 bp    DNA     circular  SYN 
DEFINITION  DNA2 (Myc-DDK-tagged)-Human DNA replication helicase 2 homolog (yeast) (DNA2)
ACCESSION   NM_001080449
VERSION     NM_001080449.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4476..4567 
                     /label="Myc-DDK"  
     misc_feature    4597..5182 
                     /label="PolyA signal"  
     misc_feature    4654..4673 
                     /label="Restriction Enzyme"  
     misc_feature    5458..6130 
                     /label="ColE1"  
     misc_feature    6560..7354 
                     /label="Kan/Neo"  
     misc_feature    7389..7691 
                     /label="SV40 ori"  
     misc_feature    7777..8254 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4458..4464 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4458 
                     /label="DNA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGACTCCC TGTATTCCCA GTCCTAAGCA AGGGAGCAAA GGCAGGGGAC 
     1081 AGAGCCCTGC TGCTCAGGTG AGGGGCCCCA CGTGGAACGC GCCGGCGCGG GAGGGGCGGC 
     1141 CTGGCGCAGG TCATTTGGGA CATCTGCGGG TTGGCGCATG CGCGCGAGGT GCGCAGGCTC 
     1201 GCGCCTTTTT CCCTTTTCTA AGCTTTCTGT GTTACCCCCG GTTCCGCTGT CTTTTCTGTC 
     1261 TACAGTTTGC GATCCCCGCG TCCAGGATGG AGCAGCTGAA CGAACTGGAG CTGCTGATGG 
     1321 AGAAGAGTTT TTGGGAGGAG GCGGAGCTGC CGGCGGAGCT ATTTCAGAAG AAAGTGGTAG 
     1381 CTTCCTTTCC AAGAACAGTT CTGAGCACAG GAATGGATAA CCGGTACCTG GTGTTGGCAG 
     1441 TCAATACTGT ACAGAACAAA GAGGGAAACT GTGAAAAGCG CCTGGTCATC ACTGCTTCAC 
     1501 AGTCACTAGA AAATAAAGAA CTATGCATCC TTAGGAATGA CTGGTGTTCT GTTCCAGTAG 
     1561 AGCCAGGAGA TATCATTCAT TTGGAGGGAG ACTGCACATC TGACACTTGG ATAATAGATA 
     1621 AAGATTTTGG ATATTTGATT CTGTATCCAG ACATGCTGAT TTCTGGCACC AGCATAGCCA 
     1681 GTAGTATTCG ATGTATGAGA AGAGCTGTCC TGAGTGAAAC TTTTAGGAGC TCTGATCCAG 
     1741 CCACACGCCA AATGCTAATT GGTACGGTTC TCCATGAGGT GTTTCAAAAA GCCATAAATA 
     1801 ATAGCTTTGC CCCAGAAAAG CTACAAGAAC TTGCTTTTCA AACAATTCAA GAAATAAGAC 
     1861 ATTTGAAGGA AATGTACCGC TTAAATCTAA GTCAAGATGA AATAAAACAA GAAGTAGAGG 
     1921 ACTATCTTCC TTCGTTTTGT AAATGGGCAG GAGATTTCAT GCATAAAAAC ACTTCGACTG 
     1981 ACTTCCCTCA GATGCAGCTC TCTCTGCCAA GTGATAATAG TAAGGATAAT TCAACATGTA 
     2041 ACATTGAAGT CGTGAAACCA ATGGATATTG AAGAAAGCAT TTGGTCCCCT AGGTTTGGAT 
     2101 TGAAAGGCAA AATAGATGTT ACAGTTGGTG TGAAAATACA TCGAGGGTAT AAAACAAAAT 
     2161 ACAAGATAAT GCCGCTGGAA CTTAAAACTG GCAAAGAATC AAATTCTATT GAACACCGTA 
     2221 GTCAGGTTGT TCTGTACACT CTACTAAGCC AAGAGAGAAG AGCTGATCCA GAGGCTGGCT 
     2281 TGCTTCTCTA CCTCAAGACT GGTCAGATGT ACCCTGTGCC TGCCAACCAT CTAGATAAAA 
     2341 GAGAATTATT AAAGCTAAGA AACCAGATGG CATTCTCATT GTTTCACCGT ATTAGCAAAT 
     2401 CTGCTACTAG ACAGAAGACA CAGCTTGCTT CTTTGCCACA AATAATTGAG GAAGAGAAAA 
     2461 CTTGTAAATA TTGTTCACAA ATTGGCAATT GTGCTCTTTA TAGCAGAGCA GTTGAACAAC 
     2521 AGATGGATTG TAGTTCAGTC CCAATTGTGA TGCTGCCCAA AATAGAAGAA GAAACCCAGC 
     2581 ATCTGAAGCA AACACACTTA GAATATTTCA GCCTTTGGTG TCTAATGTTA ACCCTGGAGT 
     2641 CACAATCGAA GGATAATAAA AAGAATCACC AAAATATCTG GCTAATGCCT GCTTCGGAAA 
     2701 TGGAGAAGAG TGGCAGTTGC ATTGGAAACC TGATTAGAAT GGAACATGTA AAGATAGTTT 
     2761 GTGATGGGCA ATATTTACAT AATTTCCAAT GTAAACATGG TGCCATACCT GTCACAAATC 
     2821 TAATGGCAGG TGACAGAGTT ATTGTAAGTG GAGAAGAAAG GTCACTGTTT GCTTTGTCTA 
     2881 GAGGATATGT GAAGGAGATT AACATGACAA CAGTAACTTG TTTATTAGAC AGAAACTTGT 
     2941 CGGTCCTTCC AGAATCAACT TTGTTCAGAT TAGACCAAGA AGAAAAAAAT TGTGATATAG 
     3001 ATACCCCATT AGGAAATCTT TCCAAATTGA TGGAAAACAC GTTTGTCAGC AAAAAACTTC 
     3061 GAGATTTAAT TATTGACTTT CGTGAACCTC AGTTTATATC CTACCTTAGT TCTGTTCTTC 
     3121 CACATGATGC AAAGGATACA GTTGCCTGCA TTCTAAAGGG TTTGAATAAG CCTCAGAGGC 
     3181 AAGCGATGAA AAAGGTACTT CTTTCAAAAG ACTACACACT CATCGTGGGT ATGCCTGGGA 
     3241 CAGGAAAAAC AACTACGATA TGTACTCTCG TAAGAATTCT CTACGCCTGT GGTTTTAGCG 
     3301 TTTTGTTGAC CAGCTATACA CACTCTGCTG TTGACAATAT TCTTTTGAAG TTAGCCAAGT 
     3361 TTAAAATAGG ATTTTTGCGT TTGGGTCAGA TTCAGAAGGT TCATCCAGCT ATCCAGCAAT 
     3421 TTACAGAGCA AGAAATTTGC AGATCAAAGT CCATTAAATC CTTAGCTCTT CTAGAAGAAC 
     3481 TCTACAATAG TCAACTTATA GTTGCAACAA CATGTATGGG AATAAACCAT CCAATATTTT 
     3541 CCCGTAAAAT TTTTGATTTT TGTATTGTGG ATGAAGCCTC TCAAATTAGC CAACCAATTT 
     3601 GTCTGGGCCC CCTTTTTTTT TCACGGAGAT TTGTGTTAGT GGGGGACCAT CAGCAGCTTC 
     3661 CTCCCCTGGT GCTAAACCGT GAAGCAAGAG CTCTTGGCAT GAGTGAAAGC TTATTCAAGA 
     3721 GGCTGGAGCA GAATAAGAGT GCTGTTGTAC AGTTAACCGT GCAGTACAGA ATGAACAGTA 
     3781 AAATTATGTC CTTAAGTAAT AAGCTGACCT ATGAGGGCAA GCTGGAGTGT GGATCAGACA 
     3841 AAGTGGCCAA TGCAGTGATA AACCTACGTC ACTTTAAAGA TGTGAAGCTG GAACTGGAAT 
     3901 TTTATGCTGA CTATTCTGAT AATCCTTGGT TGATGGGAGT ATTTGAACCC AACAATCCTG 
     3961 TTTGTTTCCT TAATACAGAC AAGGTTCCAG CGCCAGAACA AGTTGAAAAA GGTGGTGTGA 
     4021 GCAATGTAAC AGAAGCCAAA CTCATAGTTT TCCTAACCTC CATTTTTGTT AAGGCTGGAT 
     4081 GCAGTCCCTC TGATATTGGT ATTATTGCAC CGTACAGGCA GCAATTAAAG ATCATCAATG 
     4141 ATTTATTGGC ACGTTCTATT GGGATGGTCG AAGTTAATAC AGTAGACAAA TACCAAGGAA 
     4201 GGGACAAAAG TATTGTCCTA GTATCTTTTG TTAGAAGTAA TAAGGATGGA ACTGTTGGTG 
     4261 AACTCTTGAA AGATTGGCGA CGTCTTAATG TTGCTATAAC CAGAGCCAAA CATAAACTGA 
     4321 TTCTTCTGGG GTGTGTGCCC TCACTAAATT GCTATCCTCC TTTGGAGAAG CTGCTTAATC 
     4381 ATTTAAACTC AGAAAAATTA ATCATTGATC TTCCATCAAG AGAACATGAA AGTCTTTGCC 
     4441 ACATATTGGG TGACTTTCAA AGAGAAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4501 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4561 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4621 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4681 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4741 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4801 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4861 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4921 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4981 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5041 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5101 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5161 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5221 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5281 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5341 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5401 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5461 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5521 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5581 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5641 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5701 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5761 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5821 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5881 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5941 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6001 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6061 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6121 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6181 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6241 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6301 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6361 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6421 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6481 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6541 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6601 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6661 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6721 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6781 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6841 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6901 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6961 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7021 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7081 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7141 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7201 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7261 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7321 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7381 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7441 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7501 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7561 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7741 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7801 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7861 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7921 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7981 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8041 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8101 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8161 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8221 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8281 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//