LOCUS       RC217384                8511 bp    DNA     circular  SYN 
DEFINITION  EGFR (Myc-DDK-tagged)-Human epidermal growth factor receptor (EGFR), transcript variant 1
ACCESSION   NM_005228
VERSION     NM_005228.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4668..4759 
                     /label="Myc-DDK"  
     misc_feature    4789..5374 
                     /label="PolyA signal"  
     misc_feature    4846..4865 
                     /label="Restriction Enzyme"  
     misc_feature    5650..6322 
                     /label="ColE1"  
     misc_feature    6752..7546 
                     /label="Kan/Neo"  
     misc_feature    7581..7883 
                     /label="SV40 ori"  
     misc_feature    7969..8446 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4650..4656 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4650 
                     /label="EGFR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGACCCTCC GGGACGGCCG GGGCAGCGCT CCTGGCGCTG CTGGCTGCGC 
     1081 TCTGCCCGGC GAGTCGGGCT CTGGAGGAAA AGAAAGTTTG CCAAGGCACG AGTAACAAGC 
     1141 TCACGCAGTT GGGCACTTTT GAAGATCATT TTCTCAGCCT CCAGAGGATG TTCAATAACT 
     1201 GTGAGGTGGT CCTTGGGAAT TTGGAAATTA CCTATGTGCA GAGGAATTAT GATCTTTCCT 
     1261 TCTTAAAGAC CATCCAGGAG GTGGCTGGTT ATGTCCTCAT TGCCCTCAAC ACAGTGGAGC 
     1321 GAATTCCTTT GGAAAACCTG CAGATCATCA GAGGAAATAT GTACTACGAA AATTCCTATG 
     1381 CCTTAGCAGT CTTATCTAAC TATGATGCAA ATAAAACCGG ACTGAAGGAG CTGCCCATGA 
     1441 GAAATTTACA GGAAATCCTG CATGGCGCCG TGCGGTTCAG CAACAACCCT GCCCTGTGCA 
     1501 ACGTGGAGAG CATCCAGTGG CGGGACATAG TCAGCAGTGA CTTTCTCAGC AACATGTCGA 
     1561 TGGACTTCCA GAACCACCTG GGCAGCTGCC AAAAGTGTGA TCCAAGCTGT CCCAATGGGA 
     1621 GCTGCTGGGG TGCAGGAGAG GAGAACTGCC AGAAACTGAC CAAAATCATC TGTGCCCAGC 
     1681 AGTGCTCCGG GCGCTGCCGT GGCAAGTCCC CCAGTGACTG CTGCCACAAC CAGTGTGCTG 
     1741 CAGGCTGCAC AGGCCCCCGG GAGAGCGACT GCCTGGTCTG CCGCAAATTC CGAGACGAAG 
     1801 CCACGTGCAA GGACACCTGC CCCCCACTCA TGCTCTACAA CCCCACCACG TACCAGATGG 
     1861 ATGTGAACCC CGAGGGCAAA TACAGCTTTG GTGCCACCTG CGTGAAGAAG TGTCCCCGTA 
     1921 ATTATGTGGT GACAGATCAC GGCTCGTGCG TCCGAGCCTG TGGGGCCGAC AGCTATGAGA 
     1981 TGGAGGAAGA CGGCGTCCGC AAGTGTAAGA AGTGCGAAGG GCCTTGCCGC AAAGTGTGTA 
     2041 ACGGAATAGG TATTGGTGAA TTTAAAGACT CACTCTCCAT AAATGCTACG AATATTAAAC 
     2101 ACTTCAAAAA CTGCACCTCC ATCAGTGGCG ATCTCCACAT CCTGCCGGTG GCATTTAGGG 
     2161 GTGACTCCTT CACACATACT CCTCCTCTGG ATCCACAGGA ACTGGATATT CTGAAAACCG 
     2221 TAAAGGAAAT CACAGGGTTT TTGCTGATTC AGGCTTGGCC TGAAAACAGG ACGGACCTCC 
     2281 ATGCCTTTGA GAACCTAGAA ATCATACGCG GCAGGACCAA GCAACATGGT CAGTTTTCTC 
     2341 TTGCAGTCGT CAGCCTGAAC ATAACATCCT TGGGATTACG CTCCCTCAAG GAGATAAGTG 
     2401 ATGGAGATGT GATAATTTCA GGAAACAAAA ATTTGTGCTA TGCAAATACA ATAAACTGGA 
     2461 AAAAACTGTT TGGGACCTCC GGTCAGAAAA CCAAAATTAT AAGCAACAGA GGTGAAAACA 
     2521 GCTGCAAGGC CACAGGCCAG GTCTGCCATG CCTTGTGCTC CCCCGAGGGC TGCTGGGGCC 
     2581 CGGAGCCCAG GGACTGCGTC TCTTGCCGGA ATGTCAGCCG AGGCAGGGAA TGCGTGGACA 
     2641 AGTGCAACCT TCTGGAGGGT GAGCCAAGGG AGTTTGTGGA GAACTCTGAG TGCATACAGT 
     2701 GCCACCCAGA GTGCCTGCCT CAGGCCATGA ACATCACCTG CACAGGACGG GGACCAGACA 
     2761 ACTGTATCCA GTGTGCCCAC TACATTGACG GCCCCCACTG CGTCAAGACC TGCCCGGCAG 
     2821 GAGTCATGGG AGAAAACAAC ACCCTGGTCT GGAAGTACGC AGACGCCGGC CATGTGTGCC 
     2881 ACCTGTGCCA TCCAAACTGC ACCTACGGAT GCACTGGGCC AGGTCTTGAA GGCTGTCCAA 
     2941 CGAATGGGCC TAAGATCCCG TCCATCGCCA CTGGGATGGT GGGGGCCCTC CTCTTGCTGC 
     3001 TGGTGGTGGC CCTGGGGATC GGCCTCTTCA TGCGAAGGCG CCACATCGTT CGGAAGCGCA 
     3061 CGCTGCGGAG GCTGCTGCAG GAGAGGGAGC TTGTGGAGCC TCTTACACCC AGTGGAGAAG 
     3121 CTCCCAACCA AGCTCTCTTG AGGATCTTGA AGGAAACTGA ATTCAAAAAG ATCAAAGTGC 
     3181 TGGGCTCCGG TGCGTTCGGC ACGGTGTATA AGGGACTCTG GATCCCAGAA GGTGAGAAAG 
     3241 TTAAAATTCC CGTCGCTATC AAGGAATTAA GAGAAGCAAC ATCTCCGAAA GCCAACAAGG 
     3301 AAATCCTCGA TGAAGCCTAC GTGATGGCCA GCGTGGACAA CCCCCACGTG TGCCGCCTGC 
     3361 TGGGCATCTG CCTCACCTCC ACCGTGCAGC TCATCACGCA GCTCATGCCC TTCGGCTGCC 
     3421 TCCTGGACTA TGTCCGGGAA CACAAAGACA ATATTGGCTC CCAGTACCTG CTCAACTGGT 
     3481 GTGTGCAGAT CGCAAAGGGC ATGAACTACT TGGAGGACCG TCGCTTGGTG CACCGCGACC 
     3541 TGGCAGCCAG GAACGTACTG GTGAAAACAC CGCAGCATGT CAAGATCACA GATTTTGGGC 
     3601 TGGCCAAACT GCTGGGTGCG GAAGAGAAAG AATACCATGC AGAAGGAGGC AAAGTGCCTA 
     3661 TCAAGTGGAT GGCATTGGAA TCAATTTTAC ACAGAATCTA TACCCACCAG AGTGATGTCT 
     3721 GGAGCTACGG GGTGACCGTT TGGGAGTTGA TGACCTTTGG ATCCAAGCCA TATGACGGAA 
     3781 TCCCTGCCAG CGAGATCTCC TCCATCCTGG AGAAAGGAGA ACGCCTCCCT CAGCCACCCA 
     3841 TATGTACCAT CGATGTCTAC ATGATCATGG TCAAGTGCTG GATGATAGAC GCAGATAGTC 
     3901 GCCCAAAGTT CCGTGAGTTG ATCATCGAAT TCTCCAAAAT GGCCCGAGAC CCCCAGCGCT 
     3961 ACCTTGTCAT TCAGGGGGAT GAAAGAATGC ATTTGCCAAG TCCTACAGAC TCCAACTTCT 
     4021 ACCGTGCCCT GATGGATGAA GAAGACATGG ACGACGTGGT GGATGCCGAC GAGTACCTCA 
     4081 TCCCACAGCA GGGCTTCTTC AGCAGCCCCT CCACGTCACG GACTCCCCTC CTGAGCTCTC 
     4141 TGAGTGCAAC CAGCAACAAT TCCACCGTGG CTTGCATTGA TAGAAATGGG CTGCAAAGCT 
     4201 GTCCCATCAA GGAAGACAGC TTCTTGCAGC GATACAGCTC AGACCCCACA GGCGCCTTGA 
     4261 CTGAGGACAG CATAGACGAC ACCTTCCTCC CAGTGCCTGA ATACATAAAC CAGTCCGTTC 
     4321 CCAAAAGGCC CGCTGGCTCT GTGCAGAATC CTGTCTATCA CAATCAGCCT CTGAACCCCG 
     4381 CGCCCAGCAG AGACCCACAC TACCAGGACC CCCACAGCAC TGCAGTGGGC AACCCCGAGT 
     4441 ATCTCAACAC TGTCCAGCCC ACCTGTGTCA ACAGCACATT CGACAGCCCT GCCCACTGGG 
     4501 CCCAGAAAGG CAGCCACCAA ATTAGCCTGG ACAACCCTGA CTACCAGCAG GACTTCTTTC 
     4561 CCAAGGAAGC CAAGCCAAAT GGCATCTTTA AGGGCTCCAC AGCTGAAAAT GCAGAATACC 
     4621 TAAGGGTCGC GCCACAAAGC AGTGAATTTA TTGGAGCAAC GCGTACGCGG CCGCTCGAGC 
     4681 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4741 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4801 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4861 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4921 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4981 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5041 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5101 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5161 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5221 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5281 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5341 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5401 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5461 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5521 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5581 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5641 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5701 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5761 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5821 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5881 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5941 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6001 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6061 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6121 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6181 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6241 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6301 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6361 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6421 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6481 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6541 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6601 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6661 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6721 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6781 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6841 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6901 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6961 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7021 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7081 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7141 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7201 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7261 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7321 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7381 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7441 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7501 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7561 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7621 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7681 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7741 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7861 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7921 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7981 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8041 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8101 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8161 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8221 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8281 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8341 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8401 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8461 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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