LOCUS       RC217265                8418 bp    DNA     circular  SYN 
DEFINITION  ITGAE (Myc-DDK-tagged)-Human integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1, alpha polypeptide) (ITGAE)
ACCESSION   NM_002208
VERSION     NM_002208.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4575..4666 
                     /label="Myc-DDK"  
     misc_feature    4696..5281 
                     /label="PolyA signal"  
     misc_feature    4753..4772 
                     /label="Restriction Enzyme"  
     misc_feature    5557..6229 
                     /label="ColE1"  
     misc_feature    6659..7453 
                     /label="Kan/Neo"  
     misc_feature    7488..7790 
                     /label="SV40 ori"  
     misc_feature    7876..8353 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4557..4563 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4557 
                     /label="ITGAE"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCTCTTC CACACTCTGC TCTGCATAGC CAGCCTGGCC CTGCTGGCCG 
     1081 CTTTCAATGT GGATGTGGCC CGGCCCTGGC TCACGCCCAA GGGAGGTGCC CCTTTCGTGC 
     1141 TCAGCTCCCT TCTGCACCAA GACCCCAGCA CCAACCAGAC CTGGCTCCTG GTCACCAGCC 
     1201 CCAGAACCAA GAGGACACCA GGGCCCCTCC ATCGATGTTC CCTTGTCCAG GATGAAATCC 
     1261 TTTGCCATCC TGTAGAGCAT GTCCCCATCC CCAAGGGGAG GCACCGGGGA GTGACCGTTG 
     1321 TCCGGAGCCA CCACGGTGTT TTGATATGCA TTCAAGTGCT GGTCCGGCGG CCTCACAGCC 
     1381 TCAGCTCAGA ACTCACAGGC ACCTGTAGCC TCCTGGGCCC TGACCTCCGT CCCCAGGCTC 
     1441 AGGCCAACTT CTTCGACCTT GAAAATCTCC TGGATCCAGA TGCACGTGTG GACACTGGAG 
     1501 ACTGCTACAG CAACAAAGAA GGCGGTGGAG AAGACGATGT GAACACAGCC AGGCAGCGCC 
     1561 GGGCTCTGGA GAAGGAGGAG GAGGAAGACA AGGAGGAGGA GGAAGACGAG GAGGAGGAGG 
     1621 AAGCTGGCAC CGAGATTGCC ATCATCCTGG ATGGCTCAGG AAGCATTGAT CCCCCAGACT 
     1681 TTCAGAGAGC CAAAGACTTC ATCTCCAACA TGATGAGGAA CTTCTATGAA AAGTGTTTTG 
     1741 AGTGCAACTT TGCCTTGGTG CAGTATGGAG GAGTGATCCA GACTGAGTTT GACCTTCGGG 
     1801 ACAGCCAGGA TGTGATGGCC TCCCTCGCCA GAGTCCAGAA CATCACTCAA GTGGGGAGTG 
     1861 TCACCAAGAC TGCCTCAGCC ATGCAACACG TCTTAGACAG CATCTTCACC TCAAGCCACG 
     1921 GCTCCAGGAG AAAGGCATCC AAGGTCATGG TGGTGCTCAC CGATGGTGGC ATATTCGAGG 
     1981 ACCCCCTCAA CCTTACGACA GTCATCAACT CCCCCAAAAT GCAGGGTGTT GAGCGCTTTG 
     2041 CCATTGGGGT GGGAGAAGAA TTTAAGAGTG CTAGGACTGC GAGGGAACTG AACCTGATCG 
     2101 CCTCAGACCC GGATGAGACC CATGCTTTCA AGGTGACCAA CTACATGGCG CTGGATGGGC 
     2161 TGCTGAGCAA ACTGCGGTAC AACATCATCA GCATGGAAGG CACGGTTGGA GACGCCCTTC 
     2221 ACTACCAGCT GGCACAGATT GGCTTCAGTG CTCAGATCCT GGATGAGCGG CAGGTGCTGC 
     2281 TCGGCGCCGT CGGGGCCTTT GACTGGTCCG GAGGGGCGTT GCTCTACGAC ACACGCAGCC 
     2341 GCCGGGGCCG CTTCCTGAAC CAGACAGCGG CGGCGGCGGC AGACGCGGAG GCTGCGCAGT 
     2401 ACAGCTACCT GGGTTACGCT GTGGCCGTGC TGCACAAGAC CTGCAGCCTC TCCTACGTCG 
     2461 CGGGGGCTCC ACAGTACAAA CATCATGGGG CCGTGTTTGA GCTCCAGAAG GAGGGCAGAG 
     2521 AGGCCAGCTT CCTGCCAGTG CTGGAGGGAG AGCAGATGGG GTCCTATTTT GGCTCTGAGC 
     2581 TGTGCCCTGT GGACATTGAC ATGGATGGAA GCACGGACTT CTTGCTGGTG GCTGCTCCAT 
     2641 TTTACCACGT TCATGGAGAA GAAGGCAGAG TCTACGTGTA CCGTCTCAGC GAGCAGGATG 
     2701 GTTCTTTCTC CTTGGCACGC ATACTGAGTG GGCACCCCGG GTTCACCAAT GCCCGCTTTG 
     2761 GCTTTGCCAT GGCGGCTATG GGGGATCTCA GTCAGGATAA GCTCACAGAT GTGGCCATCG 
     2821 GGGCCCCCCT GGAAGGTTTT GGGGCAGATG ATGGTGCCAG CTTCGGCAGT GTGTATATCT 
     2881 ACAATGGACA CTGGGACGGC CTCTCCGCCA GCCCCTCGCA GCGGATCAGA GCCTCCACGG 
     2941 TGGCCCCAGG ACTCCAGTAC TTCGGCATGT CCATGGCTGG TGGCTTTGAT ATTAGTGGCG 
     3001 ACGGCCTTGC CGACATCACC GTGGGCACTC TGGGCCAGGC GGTTGTGTTC CGCTCCCGGC 
     3061 CTGTGGTTCG CCTGAAGGTC TCCATGGCCT TCACCCCCAG CGCACTGCCC ATCGGCTTCA 
     3121 ACGGCGTCGT GAATGTCCGT TTATGTTTTG AAATCAGCTC TGTAACCACA GCCTCTGAGT 
     3181 CAGGCCTCCG TGAGGCACTT CTCAACTTCA CGCTGGATGT GGATGTGGGG AAGCAGAGGA 
     3241 GACGGCTGCA GTGTTCAGAC GTAAGAAGCT GTCTGGGCTG CCTGAGGGAG TGGAGCAGCG 
     3301 GATCCCAGCT TTGTGAGGAC CTCCTGCTCA TGCCCACAGA GGGAGAGCTC TGTGAGGAGG 
     3361 ACTGCTTCTC CAATGCCAGT GTCAAAGTCA GCTACCAGCT CCAGACCCCT GAGGGACAGA 
     3421 CGGACCATCC CCAGCCCATC CTGGACCGCT ACACTGAGCC CTTTGCCATC TTCCAGCTGC 
     3481 CCTATGAGAA GGCCTGCAAG AATAAGCTGT TTTGTGTCGC AGAATTACAG TTGGCCACCA 
     3541 CCGTCTCTCA GCAGGAGTTG GTGGTGGGTC TCACAAAGGA GCTGACCCTG AACATTAACC 
     3601 TAACTAACTC CGGGGAAGAT TCCTACATGA CAAGCATGGC CTTGAATTAC CCCAGAAACC 
     3661 TGCAGTTGAA GAGGATGCAA AAGCCTCCCT CTCCAAACAT TCAGTGTGAT GACCCTCAGC 
     3721 CGGTTGCTTC TGTCCTGATC ATGAACTGCA GGATTGGTCA CCCCGTCCTC AAGAGGTCAT 
     3781 CTGCTCATGT TTCAGTCGTT TGGCAGCTAG AGGAGAATGC CTTTCCAAAC AGGACAGCAG 
     3841 ACATCACTGT GACTGTCACC AATTCCAATG AAAGACGGTC TTTGGCCAAC GAGACCCACA 
     3901 CCCTTCAATT CAGGCATGGC TTCGTTGCAG TTCTGTCCAA ACCATCCATA ATGTACGTGA 
     3961 ACACAGGCCA GGGGCTTTCT CACCACAAAG AATTCCTCTT CCATGTACAT GGGGAGAACC 
     4021 TCTTTGGAGC AGAATACCAG TTGCAAATTT GCGTCCCAAC CAAATTACGA GGTCTCCAGG 
     4081 TTGTAGCAGT GAAGAAGCTG ACGAGGACTC AGGCCTCCAC GGTGTGCACC TGGAGTCAGG 
     4141 AGCGCGCTTG TGCGTACAGT TCGGTTCAGC ATGTGGAAGA ATGGCATTCA GTGAGCTGTG 
     4201 TCATCGCTTC AGATAAAGAA AATGTCACCG TGGCTGCAGA GATCTCCTGG GATCACTCTG 
     4261 AGGAGTTACT AAAAGATGTA ACTGAACTGC AGATCCTTGG TGAAATATCT TTCAACAAAT 
     4321 CTCTATATGA GGGACTGAAT GCAGAGAACC ACAGAACTAA GATCACTGTC GTCTTCCTGA 
     4381 AAGATGAGAA GTACCATTCT TTGCCTATCA TCATTAAAGG CAGCGTTGGT GGACTTCTGG 
     4441 TGTTGATCGT GATTCTGGTC ATCCTGTTCA AGTGTGGCTT TTTTAAAAGA AAATATCAAC 
     4501 AACTGAACTT GGAGAGCATC AGGAAGGCCC AGCTGAAATC AGAGAATCTG CTCGAAGAAG 
     4561 AGAATACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4621 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4681 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4741 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4801 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4861 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4921 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4981 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5041 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5101 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5161 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5221 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5281 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5341 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5401 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5461 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5521 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5581 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5641 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5701 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5761 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5821 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5881 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5941 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6001 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6061 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6121 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6181 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6241 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6301 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6361 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6421 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6481 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6541 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6601 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6661 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6721 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6781 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6841 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6901 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6961 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7021 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7081 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7141 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7201 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7261 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7321 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7381 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7441 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7501 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7561 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7621 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7681 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7741 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7801 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7861 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7921 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7981 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8041 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8101 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8161 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8221 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8281 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8341 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8401 AAATTTAACG CGAATTTT
//