LOCUS       RC217100                8292 bp    DNA     circular  SYN 
DEFINITION  AP4E1 (Myc-DDK-tagged)-Human adaptor-related protein complex 4, epsilon 1 subunit (AP4E1)
ACCESSION   NM_007347
VERSION     NM_007347.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4449..4540 
                     /label="Myc-DDK"  
     misc_feature    4570..5155 
                     /label="PolyA signal"  
     misc_feature    4627..4646 
                     /label="Restriction Enzyme"  
     misc_feature    5431..6103 
                     /label="ColE1"  
     misc_feature    6533..7327 
                     /label="Kan/Neo"  
     misc_feature    7362..7664 
                     /label="SV40 ori"  
     misc_feature    7750..8227 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4431..4437 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4431 
                     /label="AP4E1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGACATA GTGGAGAAGA CGCTGACGGC GCTGCCGGGA CTCTTTCTGC 
     1081 AGAACCAGCC CGGTGGTGGG CCCGCGGCCG CCAAGGCGTC CTTCTCCTCG AGGCTGGGCA 
     1141 GCCTTGTCCG CGGCATCACA GCCCTCACCT CCAAGCACGA AGAAGAAAAA TTAATCCAGC 
     1201 AGGAACTGAG TAGTCTGAAA GCGACTGTTT CTGCTCCTAC TACAACACTG AAAATGATGA 
     1261 AGGAATGTAT GGTGAGACTT ATATATTGTG AAATGCTTGG ATATGATGCT TCCTTTGGCT 
     1321 ATATTCATGC AATCAAGTTA GCCCAACAAG GAAACCTCTT AGAAAAAAGA GTAGGTTATT 
     1381 TGGCTGTTTC CTTATTTCTA CATGAAAGTC ATGAATTATT GCTTCTCCTT GTGAATACAG 
     1441 TTGTAAAGGA TCTGCAGAGC ACTAACCTAG TAGAAGTGTG TATGGCACTG ACTGTTGTTA 
     1501 GCCAGATTTT CCCCTGCGAA ATGATTCCAG CTGTTCTTCC ATTAATAGAA GATAAACTTC 
     1561 AACATTCTAA GGAGATTGTA CGAAGAAAAG CTGTTCTGGC ATTATACAAA TTCCATCTCA 
     1621 TTGCTCCTAA TCAAGTACAA CATATTCATA TTAAGTTTCG GAAAGCACTT TGTGACAGAG 
     1681 ATGTTGGGGT CATGGCTGCC TCCTTGCATA TATATCTTAG AATGATTAAG GAGAATTCAT 
     1741 CTGGATATAA AGACTTGACT GGGAGTTTTG TAACCATTTT GAAGCAAGTA GTTGGAGGAA 
     1801 AGCTCCCAGT AGAATTCAAT TACCACAGTG TGCCAGCACC ATGGTTACAA ATTCAGCTCT 
     1861 TGAGAATACT GGGACTTCTA GGAAAAGATG ATCAAAGGAC AAGTGAATTA ATGTATGATG 
     1921 TTCTTGATGA ATCCTTACGA AGAGCTGAGT TAAATCACAA TGTCACATAT GCTATTTTGT 
     1981 TTGAATGTGT GCATACAGTC TATTCTATTT ATCCTAAATC GGAATTACTT GAGAAGGCTG 
     2041 CCAAGTGCAT TGGAAAATTT GTTCTGTCAC CTAAAATAAA TCTAAAATAT TTAGGACTGA 
     2101 AGGCTCTTAC CTATGTTATC CAACAGGATC CTACTCTGGC TCTTCAACAC CAGATGACAA 
     2161 TAATTGAATG TTTAGATCAT CCTGATCCCA TTATTAAAAG AGAGACTCTG GAACTTCTTT 
     2221 ACAGAATTAC TAATGCACAG AATATAACAG TTATTGTCCA GAAAATGCTT GAATATTTAC 
     2281 ATCAGAGCAA AGAAGAGTAT GTCATCGTCA ATTTGGTCGG CAAAATAGCA GAGCTGGCTG 
     2341 AGAAATATGC TCCTGATAAT GCATGGTTTA TTCAGACAAT GAATGCTGTG TTTTCAGTAG 
     2401 GAGGAGATGT AATGCATCCT GATATTCCCA ATAACTTTCT GAGACTACTA GCGGAAGGTT 
     2461 TTGATGATGA AACAGAAGAT CAGCAATTAA GACTCTATGC AGTTCAGTCT TATCTCACTT 
     2521 TACTGGATAT GGAAAATGTG TTCTATCCAC AGAGATTTCT TCAAGTTATG AGTTGGGTAT 
     2581 TAGGGGAATA TTCCTACCTC TTAGATAAGG AAACGCCAGA GGAAGTTATA GCTAAGCTCT 
     2641 ACAAGTTACT TATGAATGAC TCTGTGTCTT CAGAAACAAA AGCCTGGTTA ATTGCTGCTG 
     2701 TGACCAAATT GACATCTCAG GCGCACTCTT CTAATACAGT TGAGAGATTA ATCCATGAAT 
     2761 TTACCATATC TTTGGATACT TGTATGAGAC AACATGCATT TGAATTAAAA CATTTGCATG 
     2821 AGAATGTGGA ACTTATGAAG AGCTTGCTTC CAGTTGACAG GAGTTGTGAA GACTTGGTGG 
     2881 TAGATGCTTC TTTATCTTTT CTGGATGGTT TTGTGGCTGA AGGACTCAGT CAGGGTGCAG 
     2941 CGCCTTACAA ACCTCCCCAT CAACGCCAGG AGGAAAAGCT TTCTCAGGAA AAAGTTCTCA 
     3001 ATTTTGAACC ATATGGACTC TCCTTTTCTT CATCTGGCTT CACTGGACGA CAGTCTCCTG 
     3061 CTGGCATTTC TCTTGGTTCA GATGTATCTG GGAATAGTGC TGAGACAGGA CTGAAAGAGA 
     3121 CAAATAGCTT GAAGCTGGAA GGTATAAAGA AATTGTGGGG GAAAGAAGGC TATCTTCCCA 
     3181 AGAAGGAAAG CAAAACTGGT GATGAAAGTG GAGCTCTGCC TGTTCCTCAA GAGAGTATAA 
     3241 TGGAGAATGT AGATCAAGCT ATAACTAAAA AGGATCAATC TCAAGTTCTT ACCCAATCTA 
     3301 AAGAGGAGAA AGAAAAGCAG CTGCTGGCAT CATCATTATT TGTTGGTCTA GGATCAGAAA 
     3361 GTACAATCAA CCTGCTGGGA AAAGCAGATA CTGTCTCTCA CAAGTTCAGA AGGAAATCAA 
     3421 AAGTCAAAGA AGCTAAAAGT GGCGAAACAA CCAGTACTCA TAATATGACC TGTTCTTCCT 
     3481 TTAGTTCTTT GTCAAATGTG GCATATGAAG ATGATTATTA TTCGAATACT TTGCACGATA 
     3541 CAGGAGACAA GGAATTAAAG AAATTTTCTC TCACTTCAGA ACTTTTGGAT TCTGAGTCAC 
     3601 TCACAGAACT GCCCTTGGTT GAGAAATTCT CATATTGTAG TCTGTCTACA CCTTCATTGT 
     3661 TTGCTAATAA CAACATGGAA ATTTTTCACC CTCCTCAATC TACTGCAGCC TCAGTTGCCA 
     3721 AGGAAAGCTC TTTAGCTTCA TCTTTTTTGG AAGAAACTAC TGAATACATA CACTCAAATG 
     3781 CTATGGAAGT CTGTAATAAT GAAACTATAT CAGTGTCTTC TTATAAAATT TGGAAAGATG 
     3841 ATTGTTTATT GATGGTCTGG TCAGTCACTA ATAAGAGTGG TTTGGAATTG AAAAGTGCTG 
     3901 ACTTAGAAAT TTTTCCTGCA GAAAATTTCA AGGTGACTGA GCAACCTGGA TGCTGTTTGC 
     3961 CTGTAATGGA AGCAGAAAGC ACCAAAAGCT TTCAATATAG TGTGCAGATA GAAAAACCTT 
     4021 TTACAGAAGG AAATCTTACT GGTTTTATTA GTTATCATAT GATGGATACT CATTCTGCTC 
     4081 AGCTGGAATT TTCTGTAAAC TTATCACTAT TAGATTTCAT TAGACCATTA AAAATCTCAA 
     4141 GTGACGACTT TGGGAAACTC TGGTTATCCT TCGCAAATGA TGTGAAACAA AATGTAAAAA 
     4201 TGTCAGAATC TCAAGCTGCA CTTCCTTCTG CACTAAAGAC TCTGCAACAG AAACTAAGAC 
     4261 TCCATATTAT TGAGATTATA GGCAATGAAG GGCTATTGGC CTGTCAGCTG CTCCCATCCA 
     4321 TCCCCTGCTT ACTGCATTGC CGAGTTCATG CAGATGTATT AGCCCTGTGG TTCAGATCCT 
     4381 CCTGTTCTAC TCTTCCTGAC TATTTACTGT ATCAGTGTCA AAAGGTGATG GAGGGATCCA 
     4441 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4501 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4561 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4621 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4681 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4741 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4801 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4861 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4921 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4981 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5041 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5101 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5161 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5221 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5281 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5341 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5401 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5461 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5521 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5581 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5641 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5701 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5761 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5821 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5881 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5941 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6001 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6061 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6121 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6181 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6241 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6301 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6361 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6421 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6481 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6541 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6601 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6661 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6721 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6781 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6841 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6901 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6961 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7021 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7081 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7141 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7201 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7261 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7321 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7381 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7441 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7501 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7561 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7741 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7801 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7861 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7921 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7981 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8041 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8101 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8161 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8221 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8281 AACGCGAATT TT
//