LOCUS       RC217078                8073 bp    DNA     circular  SYN 
DEFINITION  LLGL1 (Myc-DDK-tagged)-Human lethal giant larvae homolog 1 (Drosophila) (LLGL1)
ACCESSION   NM_004140
VERSION     NM_004140.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4230..4321 
                     /label="Myc-DDK"  
     misc_feature    4351..4936 
                     /label="PolyA signal"  
     misc_feature    4408..4427 
                     /label="Restriction Enzyme"  
     misc_feature    5212..5884 
                     /label="ColE1"  
     misc_feature    6314..7108 
                     /label="Kan/Neo"  
     misc_feature    7143..7445 
                     /label="SV40 ori"  
     misc_feature    7531..8008 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4212..4218 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4212 
                     /label="LLGL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGAAGTTT CGGTTCCGGC GGCAGGGCGC CGACCCGCAG CGCGAGAAGC 
     1081 TCAAGCAGGA GCTTTTCGCC TTCAACAAGA CTGTGGAGCA TGGCTTCCCC AATCAGCCCA 
     1141 GCGCCCTGGC CTTCGACCCG GAACTTCGCA TCATGGCCAT CGGCACCAGG TCTGGGGCTG 
     1201 TCAAGATCTA TGGTGCACCT GGCGTGGAGT TCACAGGCCT GCACCGGGAT GCAGCCACTG 
     1261 TCACACAGAT GCACTTCTTG ACCGGCCAGG GCCGCCTCCT GTCCCTGCTT GATGACAGCA 
     1321 GTCTGCATCT CTGGGAGATT GTCCACCATA ATGGCTGTGC CCACCTGGAA GAAGCACTCA 
     1381 GTTTCCAGCT GCCCAGCCGG CCCGGCTTTG ATGGTGCCAG TGCTCCGCTC AGCCTTACCC 
     1441 GAGTCACAGT GGTCCTGCTG GTGGCTGCCG GCGACATAGC AGCCCTGGGC ACTGAGGGCA 
     1501 GCAGTGTCTT CTTCCTGGAT GTTACCACCC TGACCCTGCT CGAGGGGCAG ACGCTTGCCC 
     1561 CAGGCGAGGT TCTGCGCAGC GTGCCAGACG ACTACCGCTG TGGGAAGGCA CTGGGCCCCG 
     1621 TGGAGTCACT CCAGGGACAC CTGCGGGACC CCACAAAGAT TCTCATTGGC TACAGCCGGG 
     1681 GCCTGCTGGT CATCTGGAAC CAGGCCTCGC AGTGTGTGGA CCACATCTTC CTGGGGAACC 
     1741 AGCAGCTGGA GAGCCTATGC TGGGGGCGTG ATAGCAGCAC TGTGGTCAGC TCACACAGCG 
     1801 ATGGCAGCTA TGCTGTCTGG TCTGTGGATG CCGGCAGCTT CCCAACGCTG CAGCCCACGG 
     1861 TAGCCACCAC ACCTTACGGC CCCTTTCCCT GCAAGGCCAT TAACAAGATT CTGTGGCGGA 
     1921 ACTGTGAATC TGGGGGCCAC TTTATCATCT TCAGCGGTGG CATGCCCCGT GCCAGCTATG 
     1981 GTGACCGCCA CTGTGTAAGT GTGCTTCGAG CCGAGACATT GGTGACGCTG GACTTCACTT 
     2041 CCCGCATCAT CGACTTCTTC ACAGTGCACA GCACACGGCC CGAGGATGAA TTTGATGACC 
     2101 CCCAGGCCCT GGCTGTGCTG CTGGAAGAGG AGCTGGTGGT GCTGGACCTG CAGACTCCTG 
     2161 GCTGGCCAGC TGTGCCTGCC CCATACCTGG CCCCGCTGCA CTCCTCTGCA ATCACTTGCT 
     2221 CGGCCCACGT GGCCAGTGTC CCCGCCAAGC TGTGGGCCCG CATTGTGAGC GCTGGCGAGC 
     2281 AGCAGAGCCC CCAGCCTGTC TCCAGTGCCT TGAGCTGGCC CATCACTGGG GGCCGAAACC 
     2341 TGGCCCAGGA GCCGTCACAG CGAGGGCTGC TGCTGACGGG CCATGAGGAC GGCACCGTGA 
     2401 GGTTCTGGGA TGCCTCGGGT GTGGCGCTGC GGCCGCTCTA TAAGCTGAGC ACAGCTGGCC 
     2461 TCTTCCAGAC AGACTGTGAG CACGCTGACA GCCTGGCCCA GGCTGCCGAG GACGACTGGC 
     2521 CACCCTTCCG CAAGGTGGGC TGCTTCGATC CCTACAGTGA CGATCCCCGG CTTGGCGTGC 
     2581 AGAAGGTTGC TCTCTGCAAG TATACAGCCC AGATGGTGGT GGCTGGCACT GCAGGCCAGG 
     2641 TGCTGGTACT GGAGCTTAGT GATGTGCCGG TGGAGCAGGC GGTCAGCGTG GCCATCATAG 
     2701 ACCTCCTCCA GGACCGCGAG GGCTTCACAT GGAAGGGCCA CGAGCGGCTG AGCCCACGCA 
     2761 CGGGGCCGCT GCCCTGGCCT GCTGGCTTCC AGCCCCGTGT CCTGGTGCAG TGCCTGCCGC 
     2821 CAGCTGCTGT AACCGCTGTC ACACTCCACA CCGAGTGGAG CCTCGTGGCT TTTGGCACCA 
     2881 GTCATGGCTT TGGCCTCTTC GACTACCAGC GCAAGAGCCC TGTGCTGGCC AGGTGCACTC 
     2941 TTCACCCCAA TGACTCCCTG GCCATGGAGG GTCCGCTCTC CCGGGTGAAG TCTCTCAAGA 
     3001 AGTCACTGCG CCAGTCTTTC CGGCGCATTC GCAAGAGTCG TGTCTCTGGC AAGAAGCGGG 
     3061 CTGCTAATGC CAGCAGCAAG TTGCAGGAAG CCAATGCACA GCTGGCTGAG CAGGCCTGCC 
     3121 CCCACGACGT GGAGATGACG CCCGTGCAGC GCCGCATTGA GCCCCGCTCT GCCGATGACT 
     3181 CCTTGTCGGG TGTCGTGCGT TGCCTATACT TTGCCGACAC ATTCCTTCGA GATGGGGCCC 
     3241 ACCACGGGCC CACCATGTGG GCTGGCACCA ACTCAGGCTC TGTGTTCGCC TATGCACTGG 
     3301 AGGTGCCGGC AGCAGCAGTG GGTGGTGAGA AGCGGCCTGA GCAAGCGGTG GAGGCCGTGC 
     3361 TGGGCAAGGA GGTGCAGCTG ATGCACCGGG CGCCTGTGGT GGCCATTGCC GTGTTGGACG 
     3421 GGCGTGGCCG CCCACTGCCC GAGCCCTACG AGGCCTCACG GGACCTGGCG CAGGCACCTG 
     3481 ACATGCAGGG TGGTCACGCT GTGCTCATCG CATCTGAGGA GCAGTTCAAG GTGTTCACAC 
     3541 TGCCCAAGGT GAGCGCGAAG ACCAAGTTCA AGCTGACGGC CCATGAGGGC TGTCGTGTGC 
     3601 GCAAGGTGGC ACTGGCCACG TTTGCCAGTG TGGCCTGCGA GGACTATGCT GAGACCTGCC 
     3661 TGGCCTGCCT CACCAACCTG GGTGACGTCC ACGTCTTCTC GGTGCCTGGC CTGCGGCCCC 
     3721 AGGTGCACTA TTCCTGCATC CGGAAGGAGG ACATCAGCGG CATCGCTTCG TGCGTCTTTA 
     3781 CGCGCCATGG CCAGGGCTTT TACCTGATAT CCCCATCAGA ATTTGAACGC TTCTCCCTAA 
     3841 GTGCCCGGAA CATCACAGAG CCGCTCTGCT CTCTGGACAT TAACTGGCCC CGCGATGCCA 
     3901 CCCAGGCCAG TTACAGGATC CGAGAGTCAC CCAAGCTGAG CCAGGCTAAC GGGACCCCAA 
     3961 GCATCCTGCT GGCCCCACAG AGCCTTGATG GAAGCCCTGA TCCAGCCCAC AGCATGGGAC 
     4021 CTGACACCCC GGAGCCACCC GAGGCTGCAC TCTCACCCAT GTCCATCGAC TCAGCCACCA 
     4081 GTGCTGACAC CACGCTGGAC ACGACAGGGG ACGTCACAGT GGAAGATGTG AAGGATTTCC 
     4141 TGGGCTCCTC TGAGGAGTCA GAGAAGAACC TGAGGAACCT GGCAGAAGAC GAGGCCCACG 
     4201 CCTGTGCCAT CCTGATCAAA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4261 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4321 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4381 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4441 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4501 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4561 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4621 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4681 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4741 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4801 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4861 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4921 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4981 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5041 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5101 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5161 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5221 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5281 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5341 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5401 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5461 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5521 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5581 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5641 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5701 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5761 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5821 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5881 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5941 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6001 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6061 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6121 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6181 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6241 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6301 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6361 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6421 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6481 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6541 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6601 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6661 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6721 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6781 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6841 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6901 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6961 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7021 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7081 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7141 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7201 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7261 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7321 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7381 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7441 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7501 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7561 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7621 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7681 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7741 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7801 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7861 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7921 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7981 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8041 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//