LOCUS       RC217054                8511 bp    DNA     circular  SYN 
DEFINITION  AFF1 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2
ACCESSION   NM_005935
VERSION     NM_005935.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4668..4759 
                     /label="Myc-DDK"  
     misc_feature    4789..5374 
                     /label="PolyA signal"  
     misc_feature    4846..4865 
                     /label="Restriction Enzyme"  
     misc_feature    5650..6322 
                     /label="ColE1"  
     misc_feature    6752..7546 
                     /label="Kan/Neo"  
     misc_feature    7581..7883 
                     /label="SV40 ori"  
     misc_feature    7969..8446 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4650..4656 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4650 
                     /label="AFF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCCCAG TCAAGTTTGT ACAATGACGA CAGAAACCTG CTTCGAATTA 
     1081 GAGAGAAGGA AAGACGCAAC CAGGAAGCCC ACCAAGAGAA AGAGGCATTT CCTGAAAAGA 
     1141 TTCCCCTTTT TGGAGAGCCC TACAAGACAG CAAAAGGTGA TGAGCTGTCT AGTCGAATAC 
     1201 AGAACATGTT GGGAAACTAC GAAGAAGTGA AGGAGTTCCT TAGTACTAAG TCTCACACTC 
     1261 ATCGCCTGGA TGCTTCTGAA AATAGGTTGG GAAAGCCGAA ATATCCTTTA ATTCCTGACA 
     1321 AAGGGAGCAG CATTCCATCC AGCTCCTTCC ACACTAGTGT CCACCACCAG TCCATTCACA 
     1381 CTCCTGCGTC TGGACCACTT TCTGTTGGCA ACATTAGCCA CAATCCAAAG ATGGCGCAGC 
     1441 CAAGAACTGA ACCAATGCCA AGTCTCCATG CCAAAAGCTG CGGCCCACCG GACAGCCAGC 
     1501 ACCTGACCCA GGATCGCCTT GGTCAGGAGG GGTTCGGCTC TAGTCATCAC AAGAAAGGTG 
     1561 ACCGAAGAGC TGACGGAGAC CACTGTGCTT CGGTGACAGA TTCGGCTCCA GAGAGGGAGC 
     1621 TTTCTCCCTT AATCTCTTTG CCTTCCCCAG TTCCCCCTTT GTCACCTATA CATTCCAACC 
     1681 AGCAAACTCT TCCCCGGACG CAAGGAAGCA GCAAGGTTCA TGGCAGCAGC AATAACAGTA 
     1741 AAGGCTATTG CCCAGCCAAA TCTCCCAAGG ACCTAGCAGT GAAAGTCCAT GATAAAGAGA 
     1801 CCCCTCAAGA CAGTTTGGTG GCCCCTGCCC AGCCGCCTTC TCAGACATTT CCACCTCCCT 
     1861 CCCTCCCCTC AAAAAGTGTT GCAATGCAGC AGAAGCCCAC GGCTTATGTC CGGCCCATGG 
     1921 ATGGTCAAGA TCAGGCCCCT AGTGAATCCC CTGAACTGAA ACCACTGCCG GAGGACTATC 
     1981 GACAGCAGAC CTTTGAAAAA ACAGACTTGA AAGTGCCTGC CAAAGCCAAG CTCACCAAAC 
     2041 TGAAGATGCC TTCTCAGTCA GTTGAGCAGA CCTACTCCAA TGAAGTCCAT TGTGTTGAAG 
     2101 AGATTCTGAA GGAAATGACC CATTCATGGC CGCCTCCTTT GACAGCAATA CATACGCCTA 
     2161 GTACAGCTGA GCCATCCAAG TTTCCTTTCC CTACAAAGGA CTCTCAGCAT GTCAGTTCTG 
     2221 TAACCCAAAA CCAAAAACAA TATGATACAT CTTCAAAAAC TCACTCAAAT TCTCAGCAAG 
     2281 GAACGTCATC CATGCTCGAA GACGACCTTC AGCTCAGTGA CAGTGAGGAC AGTGACAGTG 
     2341 AACAAACCCC AGAGAAGCCT CCCTCCTCAT CTGCACCTCC AAGTGCTCCA CAGTCCCTTC 
     2401 CAGAACCAGT GGCATCAGCA CATTCCAGCA GTGCAGAGTC AGAAAGCACC AGTGACTCAG 
     2461 ACAGTTCCTC AGACTCAGAG AGCGAGAGCA GTTCAAGTGA CAGCGAAGAA AATGAGCCCC 
     2521 TAGAAACCCC AGCTCCGGAG CCTGAGCCTC CAACAACAAA CAAATGGCAG CTGGACAACT 
     2581 GGCTGACCAA AGTCAGCCAG CCAGCTGCGC CACCAGAGGG CCCCAGGAGC ACAGAGCCCC 
     2641 CACGGCGGCA CCCAGAGAGT AAGGGCAGCA GCGACAGTGC CACGAGTCAG GAGCATTCTG 
     2701 AATCCAAAGA TCCTCCCCCT AAAAGCTCCA GCAAAGCCCC CCGGGCCCCA CCCGAAGCCC 
     2761 CCCACCCCGG AAAGAGGAGC TGTCAGAAGT CTCCGGCACA GCAGGAGCCC CCACAAAGGC 
     2821 AAACCGTTGG AACCAAACAA CCCAAAAAAC CTGTCAAGGC CTCTGCCCGG GCAGGTTCAC 
     2881 GGACCAGCCT GCAGGGGGAA AGGGAGCCAG GGCTTCTTCC CTATGGCTCC CGAGACCAGA 
     2941 CTTCCAAAGA CAAGCCCAAG GTGAAGACGA AAGGACGGCC CCGGGCCGCA GCAAGCAACG 
     3001 AACCCAAGCC AGCAGTGCCC CCCTCCAGTG AGAAGAAGAA GCACAAGAGC TCCCTCCCTG 
     3061 CCCCCTCTAA GGCTCTCTCA GGCCCAGAAC CCGCGAAGGA CAATGTGGAG GACAGGACCC 
     3121 CTGAGCACTT TGCTCTTGTT CCCCTGACTG AGAGCCAGGG CCCACCCCAC AGTGGCAGCG 
     3181 GCAGCAGGAC TAGTGGCTGC CGCCAAGCCG TGGTGGTCCA GGAGGACAGC CGCAAAGACA 
     3241 GACTCCCATT GCCTTTGAGA GACACCAAGC TGCTCTCACC GCTCAGGGAC ACTCCTCCCC 
     3301 CACAAAGCTT GATGGTGAAG ATCACCCTAG ACCTGCTCTC TCGGATACCC CAGCCTCCCG 
     3361 GGAAGGGGAG CCGCCAGAGG AAAGCAGAAG ATAAACAGCC GCCCGCAGGG AAGAAGCACA 
     3421 GCTCTGAGAA GAGGAGCTCA GACAGCTCAA GCAAGTTGGC CAAAAAGAGA AAGGGTGAAG 
     3481 CAGAAAGAGA CTGTGATAAC AAGAAAATCA GACTGGAGAA GGAAATCAAA TCACAGTCAT 
     3541 CTTCATCTTC ATCCTCCCAC AAAGAATCTT CTAAAACAAA GCCCTCCAGG CCCTCCTCAC 
     3601 AGTCCTCAAA GAAGGAAATG CTCCCCCCGC CACCCGTGTC CTCGTCCTCC CAGAAGCCAG 
     3661 CCAAGCCTGC ACTTAAGAGG TCAAGGCGGG AAGCAGACAC CTGTGGCCAG GACCCTCCCA 
     3721 AAAGTGCCAG CAGTACCAAG AGCAACCACA AAGACTCTTC CATTCCCAAG CAGAGAAGAG 
     3781 TAGAGGGGAA GGGCTCCAGA AGCTCCTCGG AGCACAAGGG TTCTTCCGGA GATACTGCAA 
     3841 ATCCTTTTCC AGTGCCTTCT TTGCCAAATG GTAACTCTAA ACCAGGGAAG CCTCAAGTGA 
     3901 AGTTTGACAA ACAACAAGCA GACCTTCACA TGAGGGAGGC AAAAAAGATG AAGCAGAAAG 
     3961 CAGAGTTAAT GACGGACAGG GTTGGAAAGG CTTTTAAGTA CCTGGAAGCC GTCTTGTCCT 
     4021 TCATTGAGTG CGGAATTGCC ACAGAGTCTG AAAGCCAGTC ATCCAAGTCA GCTTACTCTG 
     4081 TCTACTCAGA AACTGTAGAT CTCATTAAAT TCATAATGTC ATTAAAATCC TTCTCAGATG 
     4141 CCACAGCGCC AACACAAGAG AAAATATTTG CTGTTTTATG CATGCGTTGC CAGTCCATTT 
     4201 TGAACATGGC GATGTTTCGT TGTAAAAAAG ACATAGCAAT AAAGTATTCT CGTACTCTTA 
     4261 ATAAACACTT CGAGAGTTCT TCCAAAGTCG CCCAGGCACC TTCTCCATGC ATTGCAAGCA 
     4321 CAGGCACACC ATCCCCTCTT TCCCCAATGC CTTCTCCTGC CAGCTCCGTA GGGTCCCAGT 
     4381 CAAGTGCTGG CAGTGTGGGG AGCAGTGGGG TGGCTGCCAC TATCAGCACC CCAGTCACCA 
     4441 TCCAGAATAT GACATCTTCC TATGTCACCA TCACATCCCA TGTTCTTACC GCCTTTGACC 
     4501 TTTGGGAACA GGCCGAGGCC CTCACGAGGA AGAATAAAGA ATTCTTTGCT CGGCTCAGCA 
     4561 CAAATGTGTG CACCTTGGCC CTCAACAGCA GTTTGGTGGA CCTGGTGCAC TATACACGAC 
     4621 AGGGTTTTCA GCAGCTACAA GAATTAACCA AAACACCTAC GCGTACGCGG CCGCTCGAGC 
     4681 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4741 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4801 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4861 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4921 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4981 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5041 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5101 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5161 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5221 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5281 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5341 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5401 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5461 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5521 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5581 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5641 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5701 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5761 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5821 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5881 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5941 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6001 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6061 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6121 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6181 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6241 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6301 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6361 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6421 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6481 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6541 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6601 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6661 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6721 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6781 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6841 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6901 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6961 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7021 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7081 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7141 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7201 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7261 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7321 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7381 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7441 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7501 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7561 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7621 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7681 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7741 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7861 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7921 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7981 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8041 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8101 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8161 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8221 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8281 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8341 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8401 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8461 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//