LOCUS       RC217053                8523 bp    DNA     circular  SYN 
DEFINITION  DGKD (Myc-DDK-tagged)-Human diacylglycerol kinase, delta 130kDa (DGKD), transcript variant 2
ACCESSION   NM_152879
VERSION     NM_152879.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4680..4771 
                     /label="Myc-DDK"  
     misc_feature    4801..5386 
                     /label="PolyA signal"  
     misc_feature    4858..4877 
                     /label="Restriction Enzyme"  
     misc_feature    5662..6334 
                     /label="ColE1"  
     misc_feature    6764..7558 
                     /label="Kan/Neo"  
     misc_feature    7593..7895 
                     /label="SV40 ori"  
     misc_feature    7981..8458 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4662..4668 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4662 
                     /label="DGKD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGCG GCGGGCGCCC CTCCGCCGGG TCCCCCGCAA CCGCCTCCGC 
     1081 CGCCGCCGCC CGAGGAGTCG TCCGACAGCG AGCCCGAGGC GGAGCCCGGC TCCCCACAGA 
     1141 AGCTCATCCG CAAGGTGTCC ACGTCGGGTC AGATCCGACA GAAGACCATC ATCAAAGAGG 
     1201 GGATGCTGAC CAAACAGAAC AATTCATTCC AGCGATCAAA AAGGAGATAC TTTAAGCTTC 
     1261 GAGGGCGAAC GCTTTACTAT GCCAAAACGG CAAAGTCAAT CATATTTGAT GAGGTGGATC 
     1321 TGACAGATGC CAGCGTAGCT GAATCCAGTA CCAAAAACGT CAACAACAGT TTTACGGTCA 
     1381 TAACTCCATG CAGGAAGCTC ATCTTGTGTG CTGATAACAG AAAAGAAATG GAAGATTGGA 
     1441 TTGCAGCATT AAAGACTGTG CAGAACAGGG AGCACTTTGA GCCCACCCAG TACAGCATGG 
     1501 ACCACTTCTC AGGGATGCAC AATTGGTACG CCTGTTCCCA CGCGAGGCCG ACCTACTGCA 
     1561 ATGTGTGCCG TGAGGCTCTG TCTGGGGTCA CGTCGCACGG GCTGTCCTGC GAGGTGTGCA 
     1621 AATTTAAGGC CCACAAGCGC TGTGCTGTGC GTGCAACCAA TAACTGCAAG TGGACCACAC 
     1681 TGGCCTCGAT CGGGAAGGAC ATCATTGAAG ATGCAGATGG GATTGCAATG CCCCACCAGT 
     1741 GGTTGGAAGG AAACCTACCT GTGAGCGCCA AGTGCACTGT GTGCGACAAG ACCTGTGGCA 
     1801 GTGTGCTGCG CCTGCAGGAC TGGCGCTGCC TCTGGTGCAA GGCCATGGTT CACACATCGT 
     1861 GTAAAGAATC CTTGCTGACC AAGTGCCCAC TTGGCCTGTG CAAAGTGTCA GTCATCCCAC 
     1921 CCACGGCTCT CAACAGCATC GACTCCGATG GGTTCTGGAA GGCCAGCTGT CCTCCTTCTT 
     1981 GCACAAGCCC ACTGTTGGTC TTCGTCAATT CAAAAAGTGG GGACAACCAG GGTGTGAAGT 
     2041 TCCTCAGAAG ATTCAAACAG CTACTAAACC CCGCCCAGGT CTTCGACCTC ATGAACGGAG 
     2101 GCCCACACCT CGGCTTACGG TTATTCCAGA AGTTTGACAC ATTCCGGATT CTGGTTTGTG 
     2161 GCGGGGATGG AAGTGTTGGC TGGGTCCTCT CCGAAATCGA CAGCCTCAAC CTTCATAAAC 
     2221 AGTGTCAGCT GGGAGTGCTG CCGCTCGGCA CAGGGAACGA CTTGGCCCGA GTACTGGGCT 
     2281 GGGGCTCAGC CTGCGATGAC GACACCCAGC TCCCCCAGAT CTTGGAGAAG TTGGAGAGAG 
     2341 CCAGCACCAA GATGCTGGAC AGGTGGAGCG TCATGGCATA CGAGGCCAAG CTCCCCCGGC 
     2401 AGGCCTCCTC CTCTACCGTC ACCGAAGACT TCAGCGAGGA TTCCGAGGTA CAGCAGATTC 
     2461 TCTTCTATGA AGACTCGGTT GCAGCCCACC TTTCTAAAAT CCTCACCTCG GACCAGCACT 
     2521 CGGTGGTCAT CTCCTCGGCC AAAGTGCTCT GTGAGACGGT GAAGGACTTC GTGGCACGGG 
     2581 TGGGGAAGGC CTATGAGAAG ACGACCGAGA GCTCGGAGGA GTCAGAGGTC ATGGCCAAGA 
     2641 AGTGCTCTGT CCTGAAAGAG AAGCTGGATT CCCTTCTCAA GACCTTGGAC GATGAGTCCC 
     2701 AGGCCTCGTC CTCTCTGCCC AACCCGCCCC CCACCATTGC CGAGGAGGCT GAAGATGGAG 
     2761 ATGGGTCGGG CAGCATCTGC GGTTCCACCG GAGACCGCTT GGTGGCATCA GCTTGCCCGG 
     2821 CCCGGCCGCA GATATTCCGG CCTCGAGAAC AGCTCATGCT GAGAGCCAAC AGCCTGAAGA 
     2881 AAGCAATTCG TCAGATCATA GAACACACAG AAAAAGCTGT CGATGAGCAG AATGCCCAGA 
     2941 CCCAGGAGCA GGAGGGCTTC GTCCTGGGCC TCTCTGAGTC AGAGGAGAAG ATGGACCACA 
     3001 GAGTGTGCCC ACCACTGTCC CACAGCGAGA GCTTCGGGGT CCCCAAGGGG AGGAGCCAGC 
     3061 GCAAAGTGTC GAAATCTCCG TGTGAAAAGC TGATCAGCAA AGGGAGTCTG TCCCTAGGCA 
     3121 GTTCTGCTTC CCTTCCGCCC CAGCCGGGAA GCCGGGACGG CCTGCCTGCG CTCAACACCA 
     3181 AGATCCTGTA CCCAAATGTC CGGGCTGGAA TGTCTGGTTC CTTACCCGGT GGCTCAGTCA 
     3241 TCAGTCGCCT GTTAATTAAT GCTGATCCCT TCAACTCTGA ACCAGAAACC CTAGAGTATT 
     3301 ACACGGAGAA ATGTGTCATG AACAACTATT TTGGCATTGG CCTGGATGCG AAGATATCCC 
     3361 TGGACTTTAA CAACAAGCGC GATGAGCACC CAGAGAAGTG CAGGAGCCGA ACCAAGAACA 
     3421 TGATGTGGTA TGGAGTTCTT GGAACCAAAG AGTTGCTGCA CAGAACCTAC AAGAACCTGG 
     3481 AGCAAAAGGT CTTGCTGGAG TGTGACGGGC GACCCATCCC ACTCCCCAGT CTTCAGGGAA 
     3541 TTGCTGTCCT TAACATTCCC AGCTATGCCG GAGGAACCAA CTTCTGGGGG GGTACCAAGG 
     3601 AAGATGATAC TTTCGCAGCT CCATCATTCG ATGACAAGAT TCTGGAGGTG GTCGCCGTGT 
     3661 TCGGCAGCAT GCAGATGGCC GTCTCTCGAG TCATCAGGCT ACAGCATCAT CGGATCGCCC 
     3721 AGTGTCGCAC GGTGAAGATC TCCATCCTTG GGGATGAGGG CGTGCCTGTG CAGGTGGACG 
     3781 GAGAGGCCTG GGTCCAGCCG CCAGGGTACA TTCGGATTGT CCACAAGAAC CGGGCACAGA 
     3841 CACTGACCAG AGACAGGGCA TTTGAGAGCA CCCTGAAGTC CTGGGAAGAC AAGCAGAAGT 
     3901 GCGAGCTGCC CCGCCCTCCA TCCTGTTCCC TGCACCCGGA GATGCTGTCC GAGGAGGAGG 
     3961 CCACCCAGAT GGACCAGTTT GGGCAGGCAG CAGGGGTCCT CATTCACAGT ATCCGAGAAA 
     4021 TAGCTCAGTC TCACCGGGAC ATGGAGCAGG AACTGGCCCA CGCCGTCAAT GCCAGCTCCA 
     4081 AGTCCATGGA CCGTGTGTAT GGCAAGCCCA GAACCACAGA GGGGCTCAAC TGCAGCTTCG 
     4141 TCCTGGAAAT GGTGAATAAC TTCAGAGCTC TGCGCAGTGA GACGGAGCTG CTGCTGTCTG 
     4201 GGAAGATGGC CCTGCAGCTG GATCCGCCTC AGAAGGAGCA GCTGGGGAGT GCTCTTGCCG 
     4261 AGATGGACCG ACAGCTCAGG AGGCTGGCAG ACACCCCGTG GCTCTGCCAG TCCGCAGAGC 
     4321 CCGGCGACGA AGAGAGTGTG ATGCTGGATC TTGCCAAGCG CAGTCGCAGT GGTAAATTCC 
     4381 GCCTCGTGAC CAAGTTTAAA AAGGAGAAAA ACAACAAGAA CAAAGAAGCT CACAGTAGCC 
     4441 TGGGAGCCCC GGTTCACCTC TGGGGGACAG AGGAGGTTGC TGCCTGGCTG GAGCACCTCA 
     4501 GTCTCTGTGA GTATAAGGAC ATCTTCACAC GGCACGACAT CCGGGGCTCT GAGCTCCTGC 
     4561 ACCTGGAGCG GAGGGACCTC AAGGACCTGG GCGTGACCAA GGTGGGCCAC ATGAAGAGGA 
     4621 TCCTGTGTGG CATCAAGGAG CTGAGCCGCA GCGCCCCCGC CGTCGAGGCC ACGCGTACGC 
     4681 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4741 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4801 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4861 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4921 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4981 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5041 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5101 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5161 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5221 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5281 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5341 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5401 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5461 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5521 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5581 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5641 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5701 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5761 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5821 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5881 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5941 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6001 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6061 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6121 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6181 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6241 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6301 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6361 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6421 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6481 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6541 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6601 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6661 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6721 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6781 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6841 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6901 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6961 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7021 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7081 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7141 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7201 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7261 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7321 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7381 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7441 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7501 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7561 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7621 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7681 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7741 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7861 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7921 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7981 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8041 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8101 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8161 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8221 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8281 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8341 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8401 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8461 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8521 TTT
//