LOCUS       RC217052                8292 bp    DNA     circular  SYN 
DEFINITION  CACNA2D4 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, alpha 2/delta subunit 4 (CACNA2D4)
ACCESSION   NM_172364
VERSION     NM_172364.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4449..4540 
                     /label="Myc-DDK"  
     misc_feature    4570..5155 
                     /label="PolyA signal"  
     misc_feature    4627..4646 
                     /label="Restriction Enzyme"  
     misc_feature    5431..6103 
                     /label="ColE1"  
     misc_feature    6533..7327 
                     /label="Kan/Neo"  
     misc_feature    7362..7664 
                     /label="SV40 ori"  
     misc_feature    7750..8227 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4431..4437 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4431 
                     /label="CACNA2D4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCTGTGGC TGCTCTGCCC TCCTTCCCCT CCCCAACCCC AGGCCCACCA 
     1081 TGCCTGCAAC TCCCAACTTC CTCGCAAACC CCAGCTCCAG CAGCCGCTGG ATTCCCCTCC 
     1141 AGCCAATGCC CGTGGCCTGG GCCTTTGTGC AGAAGACCTC GGCCCTCCTG TGGCTGCTGC 
     1201 TTCTAGGCAC CTCCCTGTCC CCTGCGTGGG GACAGGCCAA GATTCCTCTG GAAACAGTGA 
     1261 AGCTATGGGC TGACACCTTC GGCGGGGACC TGTATAACAC TGTGACCAAA TACTCAGGCT 
     1321 CTCTCTTGCT GCAGAAGAAG TACAAGGATG TGGAGTCCAG TCTGAAGATC GAGGAGGTGG 
     1381 ATGGCTTGGA GCTGGTGAGG AAGTTCTCAG AGGACATGGA GAACATGCTG CGGAGGAAAG 
     1441 TCGAGGCGGT CCAGAATCTG GTGGAAGCTG CCGAGGAGGC CGACCTGAAC CACGAATTCA 
     1501 ATGAATCCCT GGTGTTCGAC TATTACAACT CGGTCCTGAT CAACGAGAGG GACGAGAAGG 
     1561 GCAACTTCGT GGAGCTGGGC GCCGAGTTCC TCCTGGAGTC CAATGCTCAC TTCAGCAACC 
     1621 TGCCGGTGAA CACCTCCATC AGCAGCGTGC AGCTGCCCAC CAACGTGTAC AACAAAGACC 
     1681 CAGATATTTT AAATGGAGTC TACATGTCTG AAGCCTTGAA TGCTGTCTTC GTGGAGAACT 
     1741 TCCAGAGAGA CCCAACGTTG ACCTGGCAAT ATTTTGGCAG TGCAACTGGA TTCTTCAGGA 
     1801 TCTATCCAGG TATAAAATGG ACACCTGATG AGAATGGAGT CATTACTTTT GACTGCCGAA 
     1861 ACCGCGGCTG GTACATTCAA GCTGCTACTT CTCCCAAGGA CATAGTGATT TTGGTGGACG 
     1921 TGAGCGGCAG TATGAAGGGG CTGAGGATGA CTATTGCCAA GCACACCATC ACCACCATCT 
     1981 TGGACACCCT GGGGGAGAAT GACTTCGTTA ATATCATAGC GTACAATGAC TACGTCCATT 
     2041 ACATCGAGCC TTGTTTTAAA GGGATCCTCG TCCAGGCGGA CCGAGACAAT CGAGAGCATT 
     2101 TCAAACTGCT GGTGGAGGAG TTGATGGTCA AAGGTGTGGG GGTCGTGGAC CAAGCCCTGA 
     2161 GAGAAGCCTT CCAGATCCTG AAGCAGTTCC AAGAGGCCAA GCAAGGAAGC CTCTGCAACC 
     2221 AGGCCATCAT GCTCATCAGC GACGGCGCCG TGGAGGACTA CGAGCCGGTG TTTGAGAAGT 
     2281 ATAACTGGCC AGACTGTAAG GTCCGAGTTT TCACTTACCT CATTGGGAGA GAAGTGTCTT 
     2341 TTGCTGACCG CATGAAGTGG ATTGCATGCA ACAACAAAGG CTACTACACG CAGATCTCAA 
     2401 CGCTGGCGGA CACCCAGGAG AACGTGATGG AATACCTGCA CGTGCTCAGC CGCCCCATGG 
     2461 TCATCAACCA CGACCACGAC ATCATCTGGA CAGAGGCCTA CATGGACAGC AAGCTCCTCA 
     2521 GCTCGCAGGC TCAGAGCCTG ACACTGCTCA CCACTGTGGC CATGCCAGTC TTCAGCAAGA 
     2581 AGAACGAAAC GCGATCCCAT GGCATTCTCC TGGGTGTGGT GGGCTCAGAT GTGGCCCTGA 
     2641 GAGAGCTGAT GAAGCTGGCG CCCCGGTACA AGCTTGGAGT GCACGGATAC GCCTTTCTGA 
     2701 ACACCAACAA TGGCTACATC CTCTCCCATC CCGACCTCCG GCCCCTGTAC AGAGAGGGGA 
     2761 AGAAACTAAA ACCCAAACCT AACTACAACA GTGTGGATCT CTCCGAAGTG GAGTGGGAAG 
     2821 ACCAGGCTGA ATCTCTGAGA ACAGCCATGA TCAATAGGGA AACAGGTACT CTCTCGATGG 
     2881 ATGTGAAGGT TCCGATGGAT AAAGGGAAGC GAGTTCTTTT CCTGACCAAT GACTACTTCT 
     2941 TCACGGACAT CAGCGACACC CCTTTCAGTT TGGGGGTGGT GCTGTCCCGG GGCCACGGAG 
     3001 AATACATCCT TCTGGGGAAC ACGTCTGTGG AAGAAGGCCT GCATGACTTG CTTCACCCAG 
     3061 ACCTGGCCCT GGCCGGTGAC TGGATCTACT GCATCACAGA TATTGACCCA GACCACCGGA 
     3121 AGCTCAGCCA GCTAGAGGCC ATGATCCGCT TCCTCACCAG GAAGGACCCA GACCTGGAGT 
     3181 GTGACGAGGA GCTGGTCCGG GAGGTGCTGT TTGACGCGGT GGTGACAGCC CCCATGGAAG 
     3241 CCTACTGGAC AGCGCTGGCC CTCAACATGT CCGAGGAGTC TGAACACGTG GTGGACATGG 
     3301 CCTTCCTGGG CACCCGGGCT GGCCTCCTGA GAAGCAGCTT GTTCGTGGGC TCCGAGAAGG 
     3361 TCTCCGACAG GAAGTTCCTG ACACCTGAGG ACGAGGCCAG CGTGTTCACC CTGGACCGCT 
     3421 TCCCGCTGTG GTACCGCCAG GCCTCAGAGC ATCCTGCTGG CAGCTTCGTC TTCAACCTCC 
     3481 GCTGGGCAGA AGGACCAGAA AGTGCGGGTG AACCCATGGT GGTGACGGCA AGCACAGCTG 
     3541 TGGCGGTGAC CGTGGACAAG AGGACAGCCA TTGCTGCAGC CGCGGGCGTC CAAATGAAGC 
     3601 TGGAATTCCT CCAGCGCAAA TTCTGGGCGG CAACGCGGCA GTGCAGCACT GTGGATGGGC 
     3661 CGTGCACACA GAGCTGCGAG GACAGTGATC TGGACTGCTT CGTCATCGAC AACAACGGGT 
     3721 TCATTCTGAT CTCCAAGAGG TCCCGAGAGA CGGGAAGATT TCTGGGGGAG GTGGATGGTG 
     3781 CTGTCCTGAC CCAGCTGCTC AGCATGGGGG TGTTCAGCCA AGTGACTATG TATGACTATC 
     3841 AGGCCATGTG CAAACCCTCG AGTCACCACC ACAGTGCAGC CCAGCCCCTG GTCAGCCCAA 
     3901 TTTCTGCCTT CTTGACGGCG ACCAGGTGGC TGCTGCAGGA GCTGGTGCTG TTCCTGCTGG 
     3961 AGTGGAGTGT CTGGGGCTCC TGGTACGACA GAGGGGCCGA GGCCAAAAGT GTCTTCCATC 
     4021 ACTCCCACAA ACACAAGAAG CAGGACCCGC TGCAGCCCTG CGACACGGAG TACCCCGTGT 
     4081 TCGTGTACCA GCCGGCCATC CGGGAGGCCA ACGGGATCGT GGAGTGCGGG CCCTGCCAGA 
     4141 AGGTATTTGT GGTGCAGCAG ATTCCCAACA GTAACCTCCT CCTCCTGGTG ACAGACCCCA 
     4201 CCTGTGACTG CAGCATCTTC CCACCAGTGC TGCAGGAGGC GACAGAAGTC AAATATAATG 
     4261 CCTCTGTCAA ATGTGACCGG ATGCGCTCCC AGAAGCTCCG CCGGCGACCA GACTCCTGCC 
     4321 ACGCCTTCCA TCCAGAGGAG AATGCCCAGG ACTGCGGCGG CGCCTCGGAC ACCTCAGCCT 
     4381 CGCCGCCCCT ACTCCTGCTG CCTGTGTGTG CCTGGGGGCT ACTGCCCCAA CTCCTGCGGA 
     4441 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4501 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4561 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4621 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4681 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4741 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4801 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4861 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4921 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4981 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5041 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5101 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5161 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5221 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5281 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5341 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5401 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5461 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5521 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5581 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5641 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5701 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5761 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5821 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5881 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5941 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6001 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6061 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6121 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6181 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6241 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6301 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6361 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6421 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6481 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6541 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6601 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6661 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6721 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6781 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6841 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6901 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6961 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7021 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7081 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7141 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7201 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7261 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7321 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7381 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7441 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7501 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7561 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7741 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7801 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7861 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7921 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7981 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8041 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8101 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8161 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8221 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8281 AACGCGAATT TT
//