LOCUS       RC217035                7650 bp    DNA     circular  SYN 
DEFINITION  NRP1 (Myc-DDK-tagged)-Human neuropilin 1 (NRP1), transcript variant 1
ACCESSION   NM_003873
VERSION     NM_003873.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3807..3898 
                     /label="Myc-DDK"  
     misc_feature    3928..4513 
                     /label="PolyA signal"  
     misc_feature    3985..4004 
                     /label="Restriction Enzyme"  
     misc_feature    4789..5461 
                     /label="ColE1"  
     misc_feature    5891..6685 
                     /label="Kan/Neo"  
     misc_feature    6720..7022 
                     /label="SV40 ori"  
     misc_feature    7108..7585 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3789..3795 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3789 
                     /label="NRP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGGGGG CTGCCGCTCC TCTGCGCCGT GCTCGCCCTC GTCCTCGCCC 
     1081 CGGCCGGCGC TTTTCGCAAC GATAAATGTG GCGATACTAT AAAAATTGAA AGCCCCGGGT 
     1141 ACCTTACATC TCCTGGTTAT CCTCATTCTT ATCACCCAAG TGAAAAATGC GAATGGCTGA 
     1201 TTCAGGCTCC GGACCCATAC CAGAGAATTA TGATCAACTT CAACCCTCAC TTCGATTTGG 
     1261 AGGACAGAGA CTGCAAGTAT GACTACGTGG AAGTCTTCGA TGGAGAAAAT GAAAATGGAC 
     1321 ATTTTAGGGG AAAGTTCTGT GGAAAGATAG CCCCTCCTCC TGTTGTGTCT TCAGGGCCAT 
     1381 TTCTTTTTAT CAAATTTGTC TCTGACTACG AAACACATGG TGCAGGATTT TCCATACGTT 
     1441 ATGAACTTTT CAAGAGAGGT CCTGAATGTT CCCAGAACTA CACAACACCT AGTGGAGTGA 
     1501 TAAAGTCCCC CGGATTCCCT GAAAAATATC CCAACAGCCT TGAATGCACT TATATTGTCT 
     1561 TTGCGCCAAA GATGTCAGAG ATTATCCTGG AATTTGAAAG CTTTGACCTG GAGCCTGACT 
     1621 CAAATCCTCC AGGGGGGATG TTCTGTCGCT ACGACCGGCT AGAAATCTGG GATGGATTCC 
     1681 CTGATGTTGG CCCTCACATT GGGCGTTACT GTGGACAGAA AACACCAGGT CGAATCCGAT 
     1741 CCTCATCGGG CATTCTCTCC ATGGTTTTTT ACACCGACAG CGCGATAGCA AAAGAAGGTT 
     1801 TCTCAGCAAA CTACAGTGTC TTGCAGAGCA GTGTCTCAGA AGATTTCAAA TGTATGGAAG 
     1861 CTCTGGGCAT GGAATCAGGA GAAATTCATT CTGACCAGAT CACAGCTTCT TCCCAGTATA 
     1921 GCACCAACTG GTCTGCAGAG CGCTCCCGCC TGAACTACCC TGAGAATGGG TGGACTCCCG 
     1981 GAGAGGATTC CTACCGAGAG TGGATACAGG TAGACTTGGG CCTTCTGCGC TTTGTCACGG 
     2041 CTGTCGGGAC ACAGGGCGCC ATTTCAAAAG AAACCAAGAA GAAATATTAT GTCAAGACTT 
     2101 ACAAGATCGA CGTTAGCTCC AACGGGGAAG ACTGGATCAC CATAAAAGAA GGAAACAAAC 
     2161 CTGTTCTCTT TCAGGGAAAC ACCAACCCTA CAGATGTTGT GGTTGCAGTA TTCCCCAAAC 
     2221 CACTGATAAC TCGATTTGTC CGAATCAAGC CTGCAACTTG GGAAACTGGC ATATCTATGA 
     2281 GATTTGAAGT ATATGGTTGC AAGATAACAG ATTATCCTTG CTCTGGAATG TTGGGTATGG 
     2341 TGTCTGGACT TATTTCTGAC TCCCAGATCA CATCATCCAA CCAAGGGGAC AGAAACTGGA 
     2401 TGCCTGAAAA CATCCGCCTG GTAACCAGTC GCTCTGGCTG GGCACTTCCA CCCGCACCTC 
     2461 ATTCCTACAT CAATGAGTGG CTCCAAATAG ACCTGGGGGA GGAGAAGATC GTGAGGGGCA 
     2521 TCATCATTCA GGGTGGGAAG CACCGAGAGA ACAAGGTGTT CATGAGGAAG TTCAAGATCG 
     2581 GGTACAGCAA CAACGGCTCG GACTGGAAGA TGATCATGGA TGACAGCAAA CGCAAGGCGA 
     2641 AGTCTTTTGA GGGCAACAAC AACTATGATA CACCTGAGCT GCGGACTTTT CCAGCTCTCT 
     2701 CCACGCGATT CATCAGGATC TACCCCGAGA GAGCCACTCA TGGCGGACTG GGGCTCAGAA 
     2761 TGGAGCTGCT GGGCTGTGAA GTGGAAGCCC CTACAGCTGG ACCGACCACT CCCAACGGGA 
     2821 ACTTGGTGGA TGAATGTGAT GACGACCAGG CCAACTGCCA CAGTGGAACA GGTGATGACT 
     2881 TCCAGCTCAC AGGTGGCACC ACTGTGCTGG CCACAGAAAA GCCCACGGTC ATAGACAGCA 
     2941 CCATACAATC AGAGTTTCCA ACATATGGTT TTAACTGTGA ATTTGGCTGG GGCTCTCACA 
     3001 AGACCTTCTG CCACTGGGAA CATGACAATC ACGTGCAGCT CAAGTGGAGT GTGTTGACCA 
     3061 GCAAGACGGG ACCCATTCAG GATCACACAG GAGATGGCAA CTTCATCTAT TCCCAAGCTG 
     3121 ACGAAAATCA GAAGGGCAAA GTGGCTCGCC TGGTGAGCCC TGTGGTTTAT TCCCAGAACT 
     3181 CTGCCCACTG CATGACCTTC TGGTATCACA TGTCTGGGTC CCACGTCGGC ACACTCAGGG 
     3241 TCAAACTGCG CTACCAGAAG CCAGAGGAGT ACGATCAGCT GGTCTGGATG GCCATTGGAC 
     3301 ACCAAGGTGA CCACTGGAAG GAAGGGCGTG TCTTGCTCCA CAAGTCTCTG AAACTTTATC 
     3361 AGGTGATTTT CGAGGGCGAA ATCGGAAAAG GAAACCTTGG TGGGATTGCT GTGGATGACA 
     3421 TTAGTATTAA TAACCACATT TCACAAGAAG ATTGTGCAAA ACCAGCAGAC CTGGATAAAA 
     3481 AGAACCCAGA AATTAAAATT GATGAAACAG GGAGCACGCC AGGATACGAA GGTGAAGGAG 
     3541 AAGGTGACAA GAACATCTCC AGGAAGCCAG GCAATGTGTT GAAGACCTTA GACCCCATCC 
     3601 TCATCACCAT CATAGCCATG AGCGCCCTGG GGGTCCTCCT GGGGGCTGTC TGTGGGGTCG 
     3661 TGCTGTACTG TGCCTGTTGG CATAATGGGA TGTCAGAAAG AAACTTGTCT GCCCTGGAGA 
     3721 ACTATAACTT TGAACTTGTG GATGGTGTGA AGTTGAAAAA AGACAAACTG AATACACAGA 
     3781 GTACTTATTC GGAGGCAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3841 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3901 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3961 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4021 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4081 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4141 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4201 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4261 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4321 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4381 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4441 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4501 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4561 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4621 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4681 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4741 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4801 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4861 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4921 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4981 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5041 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5101 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5161 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5221 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5281 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5341 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5401 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5461 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5521 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5581 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5641 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5701 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5761 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5821 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5881 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5941 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6001 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6061 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6121 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6181 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6241 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6301 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6361 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6421 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6481 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6541 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6601 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6661 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6721 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6781 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6841 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6901 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6961 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7021 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7081 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7141 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7201 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7261 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7321 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7381 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7441 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7501 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7561 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7621 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//