LOCUS       RC217030                8805 bp    DNA     circular  SYN 
DEFINITION  EVC2 (Myc-DDK-tagged)-Human Ellis van Creveld syndrome 2 (EVC2), transcript variant 1
ACCESSION   NM_147127
VERSION     NM_147127.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4962..5053 
                     /label="Myc-DDK"  
     misc_feature    5083..5668 
                     /label="PolyA signal"  
     misc_feature    5140..5159 
                     /label="Restriction Enzyme"  
     misc_feature    5944..6616 
                     /label="ColE1"  
     misc_feature    7046..7840 
                     /label="Kan/Neo"  
     misc_feature    7875..8177 
                     /label="SV40 ori"  
     misc_feature    8263..8740 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4944..4950 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4944 
                     /label="EVC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCCCTCG GGCTCCCGGG GGCGCCCCAC GTGGGTGCTG GCCGGGGGTC 
     1081 TCCTGGCAGT GGCCCTGGCG CTGGGGGGCC GAGGCTGTCT CGGCGCCAGC TCACGTCCCC 
     1141 GCTGGCGCCC CCTCGGCGCG CAGCCACCCC GGGATCCCCA GGTGGCTCCT AGGTCTGGGC 
     1201 CCGGCCTGAG GATCCCTCCG GGGCGGAGCG GGGCGGGGCC CGAGAGCAGC ACGCAGGACT 
     1261 TGCCCTGTAT GATTTGGCCC AAAGTGGAAT GCTGTCACTT TAAGACTGCA GTGGAAGCAC 
     1321 CACTTGGAAT GAAATTGGAC AAGAAAATGG AAGTCTTCAT CCCACTCTCA ACTTCTGCAG 
     1381 CCTCTAGTGG GCCATGGGCT CATTCCTTAT TTGCTTTTAT ACCCTCCTGG CCTAAGAAGA 
     1441 ACTTATTTAA AAGAGAGTCT CCTATAACAC ACCGCCTGTA TGGGGACATT TCAAGAGAAG 
     1501 TTCAAGGGAC TTCTGAAAAT GGAGTAATTT TTCAGAAATG TGCACTGGTG TCTGGGTCGA 
     1561 GTGAAGCACA GACAGCCCGC ATATGGCTGC TTGTTAACAA CACCAAGACA ACCTCGTCAG 
     1621 CCAACCTCTC GGAGCTGCTC TTGCTGGACA GCATTGCTGG TCTCACCATT TGGGACTCTG 
     1681 TGGGAAACAG GACCTCGGAA GGATTCCAGG CTTTTAGCAA GAAGTTTCTG CAAGTGGGAG 
     1741 ATGCCTTTGC TGTCAGCTAC GCAGCCACGC TCCAGGCTGG AGACCTCGGG AACGGGGAGA 
     1801 GCCTCAAGCT TCCTGCCCAA CTCACCTTTC AGAGCTCGTC ACGGAACAGA ACACAGCTGA 
     1861 AAGTGCTTTT TTCCATAACA GCAGAAGAAA ACGTAACGGT TCTGCCGCAC CACGGCCTCC 
     1921 ACGCAGCAGG GTTCTTCATT GCCTTCCTCC TCTCCCTTGT GCTGACCTGG GCTGCCCTCT 
     1981 TCCTCATGGT TCGCTATCAG TGTCTGAAGG GAAACATGCT CACCAGACAT CGGGTTTGGC 
     2041 AGTATGAGAG CAAGCTGGAA CCCTTGCCGT TCACCTCAGC TGATGGCGTG AATGAGGACC 
     2101 TTTCCCTTAA CGACCAAATG ATAGACATTC TGTCTTCCGA GGACCCTGGG AGCATGCTTC 
     2161 AAGCCTTAGA AGAGTTGGAG ATTGCAACCC TGAATCGGGC AGATGCAGAT CTGGAGGCTT 
     2221 GTCGAACACA AATCAGCAAG GATATCATTG CCCTTCTGCT GAAAAATCTC ACCAGCAGTG 
     2281 GCCACCTCTC ACCCCAAGTA GAGAGAAAAA TGAGTGCTGT TTTCAAAAAG CAGTTTCTAT 
     2341 TGCTGGAAAA TGAAATACAA GAGGAGTACG ATCGGAAGAT GGTGGCATTG ACAGCTGAAT 
     2401 GTGACCTGGA AACAAGAAAG AAGATGGAAA ACCAGTACCA GAGAGAGATG ATGGCAATGG 
     2461 AGGAAGCAGA AGAGTTGCTG AAACGTGCTG GTGAGAGGTC TGCTGTAGAG TGCAGCAACC 
     2521 TTCTGCGGAC CCTCCATGGC CTGGAACAGG AGCACTTGAG GAAGTCTCTC GCTTTGCAAC 
     2581 AAGAAGAAGA CTTTGCCAAA GCTCACAGAC AGCTGGCTGT TTTCCAGAGA AATGAACTGC 
     2641 ACAGTATCTT TTTTACCCAG ATAAAAAGTG CTATTTTCAA AGGGGAATTG AAACCAGAGG 
     2701 CAGCTAAAAT GCTGCTGCAA AATTATTCTA AAATACAGGA GAATGTAGAA GAGTTAATGG 
     2761 ACTTTTTCCA GGCTAGTAAG AGGTATCATC TAAGTAAAAG ATTTGGCCAC AGGGAATATC 
     2821 TGGTCCAGAA CCTCCAGTCA TCAGAGACCC GTGTGCAGGG CCTTCTGAGC ACCGCTGCAG 
     2881 CCCAGCTGAC TCACCTCATT CAGAAGCACG AGAGAGCAGG GTACCTGGAT GAAGACCAAA 
     2941 TGGAAATGCT ATTGGAGCGG GCTCAGACAG AAGTCTTTTC AATCAAGCAG AAGTTGGACA 
     3001 ATGACTTAAA GCAGGAAAAG AAAAAGCTCC ACCAAAAATT AATAACTAAG AGAAGACGAG 
     3061 AGTTGCTACA AAAGCACAGG GAGCAGCGTA GGGAGCAGGC GTCCGTCGGC GAGGCCTTCC 
     3121 GAACGGTTGA GGATGCCGGC CAGTACCTGC ACCAGAAGAG GAGCCTGATG GAGGAGCACG 
     3181 GTGCCACCCT GGAGGAGCTG CAGGAGCGTC TGGACCAGGC CGCCCTGGAC GATCTCAGGA 
     3241 CCCTGACCCT TTCGCTGTTT GAAAAGGCCA CCGACGAGCT GCGGCGCCTG CAGAACTCAG 
     3301 CCATGACCCA GGAGCTGCTC AAGCGTGGGG TGCCCTGGCT CTTCCTGCAG CAGATCCTGG 
     3361 AGGAGCACGG CAAGGAGATG GCTGCACGGG CCGAGCAGCT GGAGGGGGAG GAGAGGGACA 
     3421 GGGACCAGGA GGGTGTCCAG AGCGTGAGGC AGAGACTGAA GGATGACGCT CCTGAGGCCG 
     3481 TGACAGAGGA GCAGGCAGAG CTGCGACGCT GGGAGCACCT GATCTTCATG AAGCTCTGCT 
     3541 CCTCAGTCTT CTCCCTGTCT GAAGAGGAGC TGCTCAGGAT GAGGCAGGAG GTCCATGGCT 
     3601 GCTTTGCTCA GATGGACAGG AGCTTGGCCC TCCCCAAGAT CCGGGCCCGA GTTCTGCTGC 
     3661 AGCAATTTCA GACTGCGTGG CGAGAAGCAG AGTTCGTGAA GCTGGACCAG GCCGTGGCTG 
     3721 CCCCTGAGCT GCAGCAACAG TCCAAGGTGA GAAAGTCACG GTCCAAGAGT AAAAGCAAGG 
     3781 GAGAGCTTCT GAAGAAGTGC ATCGAAGACA AAATTCACCT CTGTGAGGAA CAGGCCTCTG 
     3841 AAGACCTGGT GGAAAAGGTT CGAGGTGAAT TGCTGCGGGA GAGAGTGCAG CGGATGGAGG 
     3901 CACAGGAGGG AGGCTTTGCA CAGTCGCTTG TTGCTCTGCA GTTCCAGAAG GCGTCCCGGG 
     3961 TGACCGAGAC TCTGTCGGCC TACACCGCCC TCCTCAGCAT CCAGGACTTG CTCCTGGAAG 
     4021 AGCTGAGTGC ATCTGAGATG CTGACCAAGT CGGCCTGCAC ACAGATCCTG GAGTCGCACA 
     4081 GCCGGGAGCT CCAGGAGTTG GAGAGGAAGC TGGAGGACCA GCTGGTGCAG CAGGAGGCAG 
     4141 CCCAGCAGCA GCAGGCCCTG GCGAGCTGGC AGCAGTGGGT GGCCGATGGG CCCGGGATTC 
     4201 TGAACGAACC TGGGGAGGTG GATTCTGAAA GGCAGGTCTC TACTGTCCTG CACCAAGCCC 
     4261 TGAGCAAGAG CCAGACATTA CTGGAGCAAC ATCAGCAGTG TTTGAGAGAG GAACAACAGA 
     4321 ACAGTGTCGT GCTAGAAGAC TTGTTGGAAA ACATGGAGGC AGACACCTTT GCAACCCTGT 
     4381 GCAGCCAGGA GCTGAGACTG GCATCGTACC TGGCGAGGAT GGCCATGGTG CCCGGGGCCA 
     4441 CGCTTCGCCG GCTCCTGAGT GTGGTACTGC CCACAGCCTC ACAGCCTCAG CTGCTGGCCC 
     4501 TGCTGGATTC GGCCACCGAG AGACATGTGG ACCACGCAGC TGAGAGCGAT GGCGGAGCGG 
     4561 AGCAGGCCGA CGTGGGCAGG CGGAGGAAAC ACCAGAGCTG GTGGCAAGCC TTAGATGGCA 
     4621 AACTGCGAGG AGATCTGATA AGCAGAGGAT TAGAAAAGAT GCTGTGGGCC CGCAAGAGAA 
     4681 AGCAGAGCAT ATTAAAGAAG ACATGTCTCC CTCTCAGAGA GAGGATGATA TTCTCTGGAA 
     4741 AAGGAAGTTG GCCACACCTG TCACTGGAGC CCATTGGCGA ACTGGCCCCT GTACCCATTG 
     4801 TAGGGGCAGA AACCATTGAT CTATTAAACA CAGGAGAGAA GCTCTTTATA TTCAGAAATC 
     4861 CAAAGGAGCC AGAGATCTCA CTGCACGTTC CTCCCAGGAA AAAGAAGAAC TTTTTGAATG 
     4921 CCAAAAAGGC CATGAGGGCC TTGGGCATGG ACACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4981 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5041 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5101 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5161 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5221 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5281 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5341 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5401 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5461 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5521 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5581 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5641 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5701 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5761 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5821 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5881 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5941 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6001 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6061 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6121 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6181 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6241 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6301 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6361 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6421 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6481 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6541 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6601 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6661 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6721 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6781 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6841 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6901 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6961 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7021 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7081 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7141 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7201 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7261 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7321 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7381 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7441 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7501 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7561 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7621 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7681 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7741 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7801 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7861 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7921 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7981 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8041 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8101 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8161 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8221 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8281 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8341 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8401 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8461 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8521 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8581 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8641 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8701 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8761 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//