LOCUS       RC217019                9495 bp    DNA     circular  SYN 
DEFINITION  CLASP1 (Myc-DDK-tagged)-Human cytoplasmic linker associated protein 1 (CLASP1), transcript variant 1
ACCESSION   NM_015282
VERSION     NM_015282.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5652..5743 
                     /label="Myc-DDK"  
     misc_feature    5773..6358 
                     /label="PolyA signal"  
     misc_feature    5830..5849 
                     /label="Restriction Enzyme"  
     misc_feature    6634..7306 
                     /label="ColE1"  
     misc_feature    7736..8530 
                     /label="Kan/Neo"  
     misc_feature    8565..8867 
                     /label="SV40 ori"  
     misc_feature    8953..9430 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5634..5640 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5634 
                     /label="CLASP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCTCGC ATGGAGTCCT GCCTGGCGCA GGTGTTGCAG AAGGATGTGG 
     1081 GGAAACGATT GCAGGTTGGC CAAGAACTGA TAGACTATTT CTCAGACAAA CAGAAGTCTG 
     1141 CTGACCTTGA GCATGACCAG ACCATGTTAG ATAAACTTGT GGATGGACTT GCTACCTCTT 
     1201 GGGTGAACTC TAGCAATTAC AAGGTGGTTC TGCTGGGCAT GGACATCCTG TCCGCCCTGG 
     1261 TGACCCGGCT GCAGGATCGG TTCAAGGCGC AGATCGGCAC AGTGCTGCCA AGTCTAATAG 
     1321 ACAGACTAGG AGATGCTAAA GACTCTGTGA GGGAGCAGGA CCAAACTCTG CTGCTAAAGA 
     1381 TCATGGATCA AGCTGCTAAT CCCCAGTACG TATGGGACAG AATGCTTGGA GGCTTCAAAC 
     1441 ACAAGAATTT CCGTACTCGA GAAGGCATCT GTCTCTGCCT TATAGCAACA CTCAATGCCT 
     1501 CTGGAGCACA GACTTTAACA CTAAGCAAGA TTGTGCCACA TATATGCAAC TTACTTGGAG 
     1561 ATCCAAACAG CCAGGTTCGA GATGCAGCAA TAAACAGCTT AGTGGAAATT TACAGACATG 
     1621 TAGGAGAACG TGTGAGGGCA GATCTCAGTA AAAAAGGATT GCCACAGTCC CGGTTGAATG 
     1681 TAATTTTTAC AAAATTTGAT GAAGTCCAGA AATCTGGAAA CATGATACAA TCTGCAAATG 
     1741 ATAAAAATTT CGACGATGAA GATTCTGTGG ATGGTAACAG ACCTTCCTCT GCTAGTTCTA 
     1801 CATCATCCAA GGCTCCACCA AGTTCTCGGA GAAACGTTGG AATGGGAACC ACCCGCCGGC 
     1861 TTGGTTCATC CACCCTTGGA TCCAAGTCTT CAGCTGCAAA AGAAGGAGCT GGTGCTGTTG 
     1921 ATGAAGAGGA TTTTATTAAA GCATTTGATG ATGTACCTGT AGTACAGATT TATTCCAGCC 
     1981 GAGACCTTGA GGAATCCATA AACAAAATTA GGGAAATATT ATCTGATGAC AAGCATGATT 
     2041 GGGAGCAGAG AGTAAATGCT CTAAAAAAGA TTAGATCTTT ACTTTTGGCT GGTGCTGCTG 
     2101 AGTATGATAA CTTCTTTCAA CATTTGCGTC TTTTGGATGG AGCCTTTAAA CTCTCTGCTA 
     2161 AGGACCTGCG GTCTCAGGTA GTGCGGGAGG CTTGTATCAC GTTGGGGCAT CTGTCATCAG 
     2221 TTCTGGGGAA TAAGTTTGAC CATGGAGCTG AAGCCATTAT GCCAACTATC TTTAATTTAA 
     2281 TTCCAAACAG TGCCAAAATT ATGGCCACAT CTGGTGTTGT AGCTGTTAGG TTAATTATTC 
     2341 GGCACACACA CATCCCTAGG TTAATACCTG TCATAACAAG CAACTGTACC TCTAAGTCTG 
     2401 TCGCAGTTAG AAGGCGCTGT TTTGAATTTT TAGATTTGCT TTTACAAGAA TGGCAGACAC 
     2461 ATTCACTAGA ACGACACATA TCAGTATTAG CTGAAACAAT AAAGAAGGGA ATACATGATG 
     2521 CTGATTCCGA AGCAAGAATA GAAGCCAGAA AATGTTACTG GGGTTTCCAC AGTCACTTCA 
     2581 GCAGAGAAGC AGAGCACTTG TACCACACCT TGGAGTCCTC CTACCAGAAA GCCCTGCAGT 
     2641 CCCACCTGAA GAACTCAGAC AGCATAGTGT CTCTGCCTCA GTCAGACCGC TCATCTTCCA 
     2701 GCTCTCAAGA GAGTCTAAAT CGTCCGCTGT CTGCCAAAAG AAGTCCTACT GGAAGTACCA 
     2761 CATCTAGAGC TTCTACAGTT AGTACCAAAT CTGTGTCAAC GACTGGGTCC CTCCAGCGAT 
     2821 CTCGAAGTGA TATTGATGTG AACGCAGCAG CCAGTGCCAA ATCCAAAGTC TCCTCATCTT 
     2881 CGGGCACGAC GCCTTTCAGC TCTGCAGCAG CTTTGCCTCC AGGGTCATAC GCATCCTTAG 
     2941 GTCGGATCCG CACAAGACGG CAAAGCTCTG GGAGTGCCAC CAACGTCGCC TCTACACCTG 
     3001 ATAACCGGGG CCGCAGTCGC GCTAAAGTGG TTTCACAGTC CCAGCGATCC AGATCTGCTA 
     3061 ATCCTGCTGG TGCTGGCAGC CGGTCAAGTT CCCCAGGAAA ATTGTTGGGA AGTGGTTATG 
     3121 GTGGACTTAC TGGGGGCTCC TCACGAGGCC CACCTGTGAC ACCGTCTTCA GAAAAGCGAA 
     3181 GCAAGATTCC CAGGAGCCAG GGATGTAGCC GGGAAACAAG TCCAAACCGA ATAGGATTAG 
     3241 CACGGAGCAG CCGTATCCCT CGACCCAGCA TGAGTCAGGG GTGCAGCCGC GATACCAGCC 
     3301 GTGAGAGCAG CCGAGATACA AGCCCTGCTC GGGGCTTTCC TCCACTTGAT CGGTTTGGGC 
     3361 TTGGCCAGCC AGGAAGAATA CCTGGTTCTG TGAATGCCAT GAGAGTTCTG AGCACAAGTA 
     3421 CAGATCTTGA AGCTGCTGTT GCTGATGCTT TGAAGAAGCC TGTGAGGAGG AGATATGAGC 
     3481 CGTATGGGAT GTATTCTGAC GATGATGCCA ACAGTGATGC CTCAAGTGTT TGCTCTGAGC 
     3541 GCTCATATGG CTCCAGGAAT GGTGGCATTC CCCATTATCT GCGGCAGACT GAGGATGTAG 
     3601 CAGAAGTTCT CAACCACTGT GCTAGTTCAA ACTGGTCAGA AAGGAAAGAA GGGCTTCTGG 
     3661 GCCTGCAGAA CTTACTGAAG AGCCAAAGAA CACTGAGTCG AGTTGAACTG AAAAGGTTGT 
     3721 GTGAGATCTT CACTCGGATG TTTGCTGACC CTCATAGCAA GAGAGTTTTC AGTATGTTTT 
     3781 TGGAGACTCT TGTGGATTTT ATAATAATTC ATAAGGATGA TTTACAAGAC TGGCTTTTTG 
     3841 TTCTTCTCAC ACAATTACTT AAGAAAATGG GAGCAGATTT ACTTGGATCT GTGCAAGCAA 
     3901 AAGTTCAAAA GGCTCTAGAT GTCACAAGGG ACTCCTTTCC ATTTGATCAA CAATTTAACA 
     3961 TTTTGATGAG ATTTATTGTG GATCAAACTC AAACTCCAAA CCTCAAGGTC AAAGTTGCAA 
     4021 TCCTGAAATA CATTGAGTCT CTGGCCAGAC AGATGGATCC AACAGATTTT GTAAACTCTA 
     4081 GTGAGACAAG GCTTGCTGTT TCTAGAATCA TAACCTGGAC AACAGAACCA AAGAGTTCAG 
     4141 ACGTGAGAAA GGCAGCACAG ATTGTGCTAA TCTCTCTGTT TGAATTGAAT ACTCCTGAAT 
     4201 TTACCATGTT ACTTGGTGCC TTGCCAAAAA CATTCCAGGA TGGTGCCACC AAACTCCTGC 
     4261 ACAACCACCT CAAGAATTCC AGTAACACCA GTGTGGGCTC TCCAAGCAAT ACGATTGGCC 
     4321 GGACGCCCTC CCGACACACC AGCAGCAGGA CCAGCCCCCT GACCTCACCC ACCAACTGTT 
     4381 CCCATGGGGG TCTGTCTCCA AGTCGGTTAT GGGGTTGGAG TGCCGACGGG TTAGCGAAGC 
     4441 ACCCACCTCC CTTTTCTCAG CCTAACTCCA TCCCCACCGC TCCCTCCCAC AAGGCTCTCA 
     4501 GGCGCTCTTA CTCTCCCAGC ATGCTGGACT ATGATACAGA GAACCTGAAC TCTGAAGAAA 
     4561 TCTATAGTTC TCTACGTGGA GTTACAGAAG CCATTGAAAA GTTTAGTTTT CGAAGCCAAG 
     4621 AAGATCTGAA TGAGCCAATT AAACGAGATG GCAAAAAGGA GTGTGATATT GTGTCCCGCG 
     4681 ATGGGGGCGC TGCCTCCCCT GCCACTGAGG GCCGGGGGGG TAGTGAAGTA GAAGGAGGCC 
     4741 GGACAGCTCT GGATAACAAG ACCTCACTAC TCAACACCCA GCCTCCGCGC GCCTTCCCGG 
     4801 GGCCGCGGGC GCGAGACTAC AACCCGTACC CCTACTCAGA TGCCATCAAC ACCTACGACA 
     4861 AGACCGCCCT GAAAGAGGCT GTGTTCGATG ACGACATGGA GCAGCTTCGA GACGTGCCCA 
     4921 TCGACCATTC TGACCTGGTG GCTGACCTTC TGAAAGAGCT GTCCAACCAC AATGAGCGAG 
     4981 TGGAGGAACG GAAGGGAGCC CTGCTGGAGC TGCTCAAGAT CACGCGGGAA GACAGCCTTG 
     5041 GTGTCTGGGA GGAGCACTTC AAGACCATTC TGCTCCTGCT GCTGGAGACC CTTGGAGACA 
     5101 AAGACCATTC AATTCGAGCA CTGGCGTTAA GAGTTTTGAG GGAAATTCTG AGAAATCAAC 
     5161 CAGCAAGATT TAAAAACTAC GCCGAGCTGA CGATTATGAA GACTCTGGAA GCCCACAAAG 
     5221 ACTCCCATAA GGAGGTGGTG AGAGCGGCTG AGGAGGCTGC GTCCACACTG GCCAGTTCCA 
     5281 TCCACCCGGA GCAGTGCATC AAGGTGCTCT GCCCCATCAT CCAGACGGCC GACTACCCCA 
     5341 TCAACCTTGC TGCCATCAAG ATGCAGACCA AAGTCGTCGA GAGGATCGCA AAGGAGTCAT 
     5401 TGCTGCAGCT CCTTGTCGAC ATCATCCCAG GCTTGCTGCA GGGTTATGAC AACACCGAAA 
     5461 GTAGTGTGCG TAAGGCCAGC GTGTTTTGCT TAGTGGCAAT TTATTCCGTA ATCGGAGAAG 
     5521 ACCTGAAACC TCACCTTGCA CAGCTCACAG GGAGCAAGAT GAAGCTACTA AACTTATACA 
     5581 TAAAGAGGGC CCAGACCACC AACAGCAACA GCAGCTCCTC CTCCGATGTC TCCACGCACA 
     5641 GCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5701 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5761 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5821 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5881 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5941 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     6001 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     6061 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     6121 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     6181 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     6241 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     6301 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     6361 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     6421 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6481 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6541 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6601 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6661 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6721 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6781 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6841 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6901 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6961 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     7021 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     7081 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     7141 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     7201 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     7261 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     7321 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     7381 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     7441 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7501 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7561 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7621 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7681 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7741 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7801 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7861 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7921 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7981 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     8041 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     8101 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     8161 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     8221 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     8281 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     8341 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     8401 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     8461 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8521 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8581 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8641 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8701 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8761 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8821 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8881 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8941 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     9001 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     9061 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     9121 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     9181 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     9241 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     9301 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     9361 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     9421 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9481 TTTAACGCGA ATTTT
//